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Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society2005; 13(5); 520-529; doi: 10.1111/j.1067-1927.2005.00073.x

Comparative study on microvascular occlusion and apoptosis in body and limb wounds in the horse.

Abstract: Wound repair in horse limbs is often complicated by exuberant granulation tissue, a condition characterized by excessive fibroplasia and scarring and that resembles hypertrophic scars and keloids in man. The aim of this study was to compare microvascular occlusion and apoptosis in wounds of the limb with those of the body, which heal normally. Five 6.25 cm(2) wounds were created on both forelimbs and on the body of six horses. One limb was bandaged to stimulate excessive fibroplasia. Weekly biopsies were evaluated histologically and immunohistochemically for mutant p53 protein by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling to localize and quantify apoptosis, and by electron microscopy to measure microvessel luminal diameters. Histologic examination revealed protracted inflammation as well as slowed epithelialization and deficient fibroblast orientation in limb wounds, particularly those with excessive fibroplasia. Microvessels were occluded significantly more often in limb wounds, and the balance of apoptotic signals was altered against apoptosis in the former, although this could not be confirmed quantitatively. Data suggest that microvascular occlusion and a dysregulated apoptotic process may be involved in the excessive accumulation of extracellular matrix within limb wounds. This might provide a basis for the development of targeted therapies to prevent and treat excessive fibroplasia and extensive scarring in horses.
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Publication Date: 2005-09-24 PubMed ID: 16176461DOI: 10.1111/j.1067-1927.2005.00073.xGoogle Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research focuses on understanding why horses often experience complications in limb wound healing processes due to overactive scar tissue formation. The study compares the role of microvascular blockage and cell death regulation in wounds on the limbs and bodies of horses, to possibly inform the development of specialized treatments for preventing and managing excessive scar tissue formation.

Objective and Methodology

  • The aim of the study was to compare microvascular occlusion (blockage of tiny blood vessels) and apoptosis (programmed cell death) in horse wounds on the limb (which often heal with extensive scarring) and those of the body (which heal as expected).
  • Researchers created 6.25 cm(2) wounds on the body and both forelimbs of six horses. One forelimb on each horse was bandaged to stimulate an overproduction of scarring tissue known as fibroplasia.
  • Biopsies from these wounds were taken weekly and examined using histology (study of microscopic structures in tissues), immunohistochemistry (study of antigen in tissues), and electron microscopy (a high-resolution microscope).
  • The examination involved localizing and quantifying apoptosis, measuring microvessel diameters, and checking for the presence of the mutant p53 protein – an important protein in preventing cancer.

Findings

  • Inflammation lasted longer, and both wound closure by the epithelium and the alignment of fibroblasts (the cells that produce collagen and other fibres) were slowed in the limb wounds, especially those with excessive fibroplasia.
  • Microvessels were more frequently blocked in limb wounds than in body wounds.
  • There was also an imbalance in apoptotic signals, leaning against apoptosis in limb wounds, though this couldn’t be quantitatively confirmed.

Implications

  • The findings suggest a potential involvement of microvascular occlusion and a disrupted apoptotic process in the excessive accumulation of the extracellular matrix within limb wounds. This overaccumulation can lead to extensive scarring and a delay in healing.
  • This study could pave the way for the development of targeted therapies to prevent and treat excessive fibroplasia and extensive scarring in horses.

Cite This Article

APA
Lepault E, Céleste C, Doré M, Martineau D, Theoret CL. (2005). Comparative study on microvascular occlusion and apoptosis in body and limb wounds in the horse. Wound Repair Regen, 13(5), 520-529. https://doi.org/10.1111/j.1067-1927.2005.00073.x

Publication

Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society
ISSN: 1067-1927
NlmUniqueID: 9310939
Country: United States
Language: English
Volume: 13
Issue: 5
Pages: 520-529

Researcher Affiliations

Lepault, Elodie
  • Department of Veterinary Biomedicine, Faculty of Veterinary Medicine, University of Montreal, Saint-Hyacinthe, Q, Canada.
Céleste, Christophe
    Doré, Monique
      Martineau, Daniel
        Theoret, Christine L

          MeSH Terms

          • Animals
          • Apoptosis / physiology
          • Cicatrix / physiopathology
          • Extremities
          • Female
          • Granulation Tissue / blood supply
          • Granulation Tissue / physiopathology
          • Horses
          • Microcirculation / physiopathology
          • Skin / blood supply
          • Skin / injuries
          • Skin / physiopathology
          • Thoracic Wall
          • Wound Healing / physiology

          Citations

          This article has been cited 17 times.
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            pubmed: 16850943
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