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Animals : an open access journal from MDPI2024; 14(15); 2237; doi: 10.3390/ani14152237

Comparative Study on the Sperm Proteomes of Horses and Donkeys.

Abstract: The reproductive performance of horse sperm and donkey sperm has been reported to differ. Sperm proteins play a crucial role in sperm viability and fertility. Although differences between species are known, no prior study has investigated disparities in the sperm proteome between horses and donkeys. Therefore, this study characterized and compared the sperm proteomes of horses and donkeys using 4D-DIA mass spectrometry technology. We identified 3436 proteins in horse sperm and 3404 proteins in donkey sperm. Of these, 3363 proteins were expressed in both horse and donkey sperm, with 73 proteins being specifically expressed in horse sperm, and 41 in donkey sperm. According to data analysis, donkeys exhibited a greater percentage of motility and progressive movement in straight-line sperm than horses, as well as lower percentages of static and slow sperm than horses. Joint analysis of the results from the horse and donkey sperm proteomes and their CEROS II-read parameters demonstrated a possible association between sperm proteins and their sperm viability patterns. These findings suggest that there are discrepancies in the expression levels and protein compositions of horse and donkey sperm and that certain specific proteins may be responsible for the differences in performance between these two species.
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Publication Date: 2024-07-31 PubMed ID: 39123763PubMed Central: PMC11311092DOI: 10.3390/ani14152237Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study compared the protein profiles, or proteomes, of horse and donkey sperm to understand the differences in their reproductive performance. They found specific proteins that are only present in each species, which may contribute to differing sperm viability patterns and mobility.

Objective and Methodology of the Study

  • The study aimed to examine the differences in the sperm proteomes, the entire set of proteins expressed by an organism, of horses and donkeys. This was instigated by an understanding that the reproductive performance differs between the two species.
  • Using 4D-DIA mass spectrometry technology, an advanced technique to identify and quantify proteins, the sperm proteomes of horses and donkeys were characterized and compared.

Findings from the Comparative Analysis

  • The study identified a total of 3436 proteins in horse sperm and 3404 proteins in donkey sperm.
  • Out of these, 3363 proteins were found to be common in both horse and donkey sperm. Additionally, 73 proteins were exclusively expressed in horse sperm, and 41 in donkey sperm.
  • According to their analysis, donkeys showed a higher percentage of mobile and progressively moving sperm compared to horses. On the other hand, horses had a higher percentage of non-moving and slow-moving sperm.

Interpretation and Implications of the Findings

  • Data from the horse and donkey sperm proteomes, along with parameters from CEROS II-read, a computer-assisted sperm analysis system, suggest a potential relationship between specific sperm proteins and patterns in sperm viability.
  • The differences in protein expression levels and compositions between horse and donkey sperm may be responsible for the varying reproductive performance between the two species.
  • The findings also hint towards the role of certain specific proteins as key drivers for the observed discrepancies, though further research is needed to confirm this assumption.

This important study paves the way for a more comprehensive understanding of reproductive performance across different species, which could eventually aid in the improvement of breeding strategies in the future.

Cite This Article

APA
Ren H, Wen X, He Q, Yi M, Dugarjaviin M, Bou G. (2024). Comparative Study on the Sperm Proteomes of Horses and Donkeys. Animals (Basel), 14(15), 2237. https://doi.org/10.3390/ani14152237

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 14
Issue: 15
PII: 2237

Researcher Affiliations

Ren, Hong
  • Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
Wen, Xin
  • Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
He, Qianqian
  • Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
Yi, Minna
  • Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
Dugarjaviin, Manglai
  • Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.
Bou, Gerelchimeg
  • Inner Mongolia Agricultural University, Hohhot 010018, China.
  • Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot 010018, China.

Grant Funding

  • BR220402 / the Basic scientific research operating expenses project of universities directly under the Inner Mongolia Autonomous Region
  • NJZY23117 / the science and technology research projects of universities In inner Mongolia Autonomous Region
  • DC2300001263 / the 2022 annual high-level talent research support fund project for local public institutions in Inner Mongolia Autonomous Region
  • RK2300003392 / the research initiation grant program at Inner Mongolia Agricultural University

Conflict of Interest Statement

The authors declare no conflicts of interest.

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Citations

This article has been cited 4 times.
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