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Home/Equine Research Bank/Animals (Basel)/ProAKAP4 Concentration Is Related to Sperm Motility and Moti...
Animals : an open access journal from MDPI2022; 12(23); 3417; doi: 10.3390/ani12233417

ProAKAP4 Concentration Is Related to Sperm Motility and Motile Sperm Subpopulations in Frozen-Thawed Horse Semen.

Abstract: ProAKAP4 is the precursor of AKAP4 (A-kinase Anchor protein 4), the main structural protein of the fibrous sheath of sperm. The amount of proAKAP4 reflects the ability of spermatozoa to maintain the flagellum activity and functionality up to the site of fertilization and is positively correlated with progressive motility in several mammalian species. The aim of this study was to investigate the relationship between proAKAP4 concentration with horse sperm motility descriptors and spermatic motile subpopulations. For this purpose, a total of 48 ejaculates from 13 different stallions were analyzed. Spermatic motility descriptors were obtained by the CASA system, and four motile subpopulations (SP) with specific motility patterns were statistically identified. ProAKAP4 concentrations were evaluated by ELISA. The relationship between motility descriptors of sperm subpopulations and proAKAP4 concentrations was evaluated. Following a hierarchical cluster statistical analysis, ejaculates were divided into two groups according to their proAKAP4 concentrations, either having low proAKAP4 concentrations (5.06−35.61 ng/10M spz; n = 23) or high (39.92−82.23 ng/10M spz; n = 25) proAKAP4 concentrations (p < 0.001). ProAKAP4 concentrations were positively correlated (p < 0.05) with total and progressive motility, as well as with parameters of velocity. ProAKAP4 amount also showed a negative correlation (p < 0.05) with sperm motile subpopulation number 3, which was the subpopulation with the lowest velocity parameters. In conclusion, proAKAP4 concentration in stallion semen positively reflects sperm progressive motility with the functional velocity kinematic descriptors. Concentrations of proAKAP4 higher than 37.77 ng/10M spz were correlated with a very good quality frozen/thawed stallion semen.
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Publication Date: 2022-12-04 PubMed ID: 36496938PubMed Central: PMC9738597DOI: 10.3390/ani12233417Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study looked into how the levels of the precursor protein proAKAP4 in horse sperm are linked with their mobility, including for different subpopulations of moving sperm. The research found that higher levels of this protein are positively associated with better sperm movement and speed, suggesting it could be an indicator of good quality sperm.

Overview of Research Methodology

  • The researchers’ main objective was to explore the relationship between the concentration of proAKAP4 and measures of sperm motility, or movement, in horses. They also wanted to see how proAKAP4 levels related to specific subgroups of actively moving sperm.
  • A total of 48 sperm samples were examined for the study. These samples were collected from 13 different stallions.
  • The sample’s sperm motility details were gathered using a CASA system, a type of technology used for advanced sperm analysis.
  • Four distinct motile subpopulations of sperm, each with unique movement patterns, were identified for the investigation.
  • To measure the concentration of proAKAP4 in the samples, the team used a method called ELISA, a common test in biomedical research to detect and measure proteins.

Findings of the Study

  • The sperm samples were categorized into two groups using a statistical method known as hierarchical cluster analysis. This division was based on their proAKAP4 concentrations, with one group having low proAKAP4 concentrations (5.06−35.61 ng/10M spz; n = 23), and the other having high proAKAP4 concentrations (39.92−82.23 ng/10M spz; n = 25).
  • The study found that higher levels of proAKAP4 were positively correlated with both total and progressive sperm motility. This means that a higher concentration of proAKAP4 was linked with better overall and forward movement of the sperm.
  • proAKAP4 concentrations were also positively associated with the speed parameters of the sperm.
  • On the flip side, a negative relationship was observed between proAKAP4 levels and the third motile sperm subpopulation, which was identified as having the slowest movement parameters.

Conclusion of the Study

  • Based on these findings, the study concluded that in horse semen, a higher concentration of proAKAP4 reflects better progressive movement of sperm and their functional speed kinematic descriptors – essentially, the different measures of how they move.
  • ProAKAP4 concentrations higher than 37.77 ng/10M spz were found to be correlated with very good quality frozen/thawed stallion semen. This suggests that the proAKAP4 level could potentially be used as a marker for the quality of stallion sperm, particularly for samples that are frozen and then thawed out for use in breeding.

Cite This Article

APA
Dordas-Perpinyu00e0 M, Yanez-Ortiz I, Sergeant N, Mevel V, Bruyas JF, Catalu00e1n J, Delehedde M, Briand-Amirat L, Miru00f3 J. (2022). ProAKAP4 Concentration Is Related to Sperm Motility and Motile Sperm Subpopulations in Frozen-Thawed Horse Semen. Animals (Basel), 12(23), 3417. https://doi.org/10.3390/ani12233417

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 12
Issue: 23
PII: 3417

Researcher Affiliations

Dordas-Perpinyu00e0, Marta
  • Oniris, Nantes-Atlantic College of Veterinary Medicine, 44300 Nantes, France.
  • Equine Reproduction Service, Department of Animal Medicine and Surgery, Faculty of Veterinary Sciences, Autonomous University of Barcelona, 08193 Cerdanyola del Vallu00e8s, Spain.
Yanez-Ortiz, Ivan
  • Equine Reproduction Service, Department of Animal Medicine and Surgery, Faculty of Veterinary Sciences, Autonomous University of Barcelona, 08193 Cerdanyola del Vallu00e8s, Spain.
Sergeant, Nicolas
  • CHRU of Lille, University of Lille, INSERM, UMRS-1172, 59045 Lille, France.
  • SPQI S.A.S, 59000 Lille, France.
Mevel, Vincent
  • Oniris, Nantes-Atlantic College of Veterinary Medicine, 44300 Nantes, France.
Bruyas, Jean-Franu00e7ois
  • Oniris, Nantes-Atlantic College of Veterinary Medicine, 44300 Nantes, France.
Catalu00e1n, Jaime
  • Equine Reproduction Service, Department of Animal Medicine and Surgery, Faculty of Veterinary Sciences, Autonomous University of Barcelona, 08193 Cerdanyola del Vallu00e8s, Spain.
Delehedde, Maryse
  • SPQI S.A.S, 59000 Lille, France.
Briand-Amirat, Lamia
  • Oniris, Nantes-Atlantic College of Veterinary Medicine, 44300 Nantes, France.
Miru00f3, Jordi
  • Equine Reproduction Service, Department of Animal Medicine and Surgery, Faculty of Veterinary Sciences, Autonomous University of Barcelona, 08193 Cerdanyola del Vallu00e8s, Spain.

Conflict of Interest Statement

Maryse Delehedde and Nicolas Sergeant are cofounders of SPQI company.

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Citations

This article has been cited 1 times.
  1. Ribas-Maynou J, Barranco I, Salas-Huetos A. Sperm Quality and Fertility of Livestock Animals.. Animals (Basel) 2023 Feb 9;13(4).
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