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Home / Equine Research Bank / Vet Microbiol / Comparing the genetic diversity of ORF30 of Australian isola...
Veterinary microbiology2013; 169(1-2); 50-57; doi: 10.1016/j.vetmic.2013.12.007

Comparing the genetic diversity of ORF30 of Australian isolates of 3 equid alphaherpesviruses.

Abstract: A single nucleotide polymorphism (SNP) has been previously associated with EHV-1 neurological disease in several countries around the world. This disease is very uncommon in Australia and little information is available about the presence of this SNP in Australian EHV-1 isolates. The ORF30 sequence of 66 Australian EHV-1 isolates was determined and the genotype was compared to the disease manifestation of the case from which the virus was isolated. Of the 66 isolates, 61 were from cases of abortion and 5 were cases associated with equine herpesvirus myeloencephalopathy (EHM). There was no association between pathotype and genotype in these isolates. In total, 64 of the 66 isolates encoded N752, including 4 isolates from EHM cases. The ORF30 sequence was also determined for 14 EHV-4 isolates, including 2 isolates from confirmed EHV-4 abortion cases. All 14 EHV-4 isolates had aspartic acid at the position equivalent to EHV-1 AA752. Aspartic acid was also confirmed in this position for the single isolate of AHV-3 sequenced in this study. The nucleotide sequence of ORF68 was also determined and showed considerable genetic heterogeneity in the EHV-1 isolates, however, this ORF was highly conserved among the 14 EHV-4 isolates sequenced, with only one SNP identified among 7 isolates. These results confirm that the EHV1 ORF30 N752 is unique and that the D752 sequence is most likely to be the true parent strain of this virus. We suggest that the abortigenic form of EHV-1 should be considered to be the more recently emerged mutant.
Copyright © 2014 Elsevier B.V. All rights reserved.
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Publication Date: 2013-12-25 PubMed ID: 24418044DOI: 10.1016/j.vetmic.2013.12.007Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

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This study investigated the genetic diversity of ORF30 in Australian isolates of three different equid alphaherpesviruses and found no correlation between a certain single nucleotide polymorphism (SNP), previously connected with neurological disease, and pathogenicity in these isolates.

Overview of the Study

  • The study analyzed the ORF30 sequence in 66 Australian equid alphaherpesvirus 1 (EHV-1) isolates to determine the presence of a specific single nucleotide polymorphism (SNP), previously associated with neurological disease. This SNP, however, is rarely seen in Australia, hence the need for further investigation.
  • Of these 66 isolates, 61 came from abortion cases and 5 were associated with equine herpesvirus myeloencephalopathy (EHM), a disorder causing inflammation of the brain and spinal cord in horses. The researchers examined if there was a link between the genotype (the SNP) and the phenotype (EHM or abortion).

Research Findings

  • No correlation was found between the disease manifestation (phenotype) and the presence of the SNP (genotype). This reveals that the SNP does not necessarily indicate the type of disease.
  • However, it was discovered that 64 of the 66 analyzed isolates of herpesvirus encoded N752 in the ORF30 region, including four isolates from horses with EHM.
  • In addition to EHV-1, the researchers also analyzed the ORF30 sequence in 14 isolates of EHV-4, 2 of which caused abortion. It was found that all of these isolates had aspartic acid at the equivalent position associated with the SNP in EHV-1.
  • The researchers also investigated the single isolate of the AHV-3 virus sequenced and found the same aspartic acid residue.

Implications of the Findings

  • The researchers concluded that the presence of N752 in the ORF30 region might be a unique identifier of EHV-1, suggesting that the D752 sequence might indicate the original strain of this virus before mutation.
  • They also suspected that the strain of EHV-1 causing abortion could be a more recent mutation of the virus. However, more research is needed in this area to confirm these hypotheses.

Cite This Article

APA
Cuxson JL, Hartley CA, Ficorilli NP, Symes SJ, Devlin JM, Gilkerson JR. (2013). Comparing the genetic diversity of ORF30 of Australian isolates of 3 equid alphaherpesviruses. Vet Microbiol, 169(1-2), 50-57. https://doi.org/10.1016/j.vetmic.2013.12.007

Publication

Veterinary microbiology
ISSN: 1873-2542
NlmUniqueID: 7705469
Country: Netherlands
Language: English
Volume: 169
Issue: 1-2
Pages: 50-57
PII: S0378-1135(13)00585-3

Researcher Affiliations

Cuxson, Jennifer L
  • Centre for Equine Infectious Disease, Faculty of Veterinary Science, The University of Melbourne, Victoria 3010, Australia.
Hartley, Carol A
  • Centre for Equine Infectious Disease, Faculty of Veterinary Science, The University of Melbourne, Victoria 3010, Australia.
Ficorilli, Nino P
  • Centre for Equine Infectious Disease, Faculty of Veterinary Science, The University of Melbourne, Victoria 3010, Australia.
Symes, Sally J
  • Centre for Equine Infectious Disease, Faculty of Veterinary Science, The University of Melbourne, Victoria 3010, Australia.
Devlin, Joanne M
  • Centre for Equine Infectious Disease, Faculty of Veterinary Science, The University of Melbourne, Victoria 3010, Australia.
Gilkerson, James R
  • Centre for Equine Infectious Disease, Faculty of Veterinary Science, The University of Melbourne, Victoria 3010, Australia. Electronic address: jrgilk@unimelb.edu.au.

MeSH Terms

  • Abortion, Veterinary / virology
  • Amino Acid Sequence
  • Animals
  • Australia
  • Base Sequence
  • Female
  • Genes, Viral
  • Genetic Variation
  • Herpesviridae Infections / veterinary
  • Herpesviridae Infections / virology
  • Herpesvirus 1, Equid / genetics
  • Herpesvirus 1, Equid / isolation & purification
  • Herpesvirus 4, Equid / genetics
  • Herpesvirus 4, Equid / isolation & purification
  • Horse Diseases / virology
  • Horses
  • Molecular Sequence Data
  • Open Reading Frames
  • Polymorphism, Single Nucleotide
  • Pregnancy
  • Sequence Analysis, DNA
  • Varicellovirus / genetics
  • Varicellovirus / isolation & purification
  • Viral Proteins / chemistry
  • Viral Proteins / genetics

Citations

This article has been cited 9 times.
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  8. Ryt-Hansen P, Johansen VK, Cuicani MM, Larsen LE, Hansen S. Outbreak of equine herpesvirus 4 (EHV-4) in Denmark: tracing patient zero and viral characterization. BMC Vet Res 2024 Jul 3;20(1):287.
    doi: 10.1186/s12917-024-04149-xpubmed: 38961400google scholar: lookup
  9. Öhrmalm J, Cholleti H, Theelke AK, Berg M, Gröndahl G. Divergent strains of EHV-1 in Swedish outbreaks during 2012 to 2021. BMC Vet Res 2024 Jun 22;20(1):270.
    doi: 10.1186/s12917-024-04096-7pubmed: 38909196google scholar: lookup
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