Comparison between six parasitological methods for diagnosis of Trypanosoma evansi in the subtropical area of Argentina.
Abstract: In a total of 165 blood samples from horses in the Province of Formosa (Argentina), the diagnosis for equine trypanosomiasis (T. evansi) was made using Giemsa-stained smears (GSS), wet blood films (WBF), Strout's concentration method (SCM), haematocrit centrifuge technique (HCT), buffy coat method (BCM) and mouse inoculation of blood (MBI). Trypanosoma evansi was demonstrated in 52 samples. Mouse inoculation gave a sensitivity of 88.2%; HCT 71.1%; BCM 63.4%; WBF 53.8%; SCM 46.1% and GSS 45.6%. No single method alone was totally effective. The haematocrit centrifuge technique, mouse inoculation of blood and Giemsa-stained smears were proposed as the most effective diagnostic combination.
Publication Date: 1990-05-01 PubMed ID: 2382382DOI: 10.1016/0304-4017(90)90102-hGoogle Scholar: Lookup
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- Comparative Study
- Journal Article
Summary
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This research article provides a comparison of six different parasitic diagnostic methods for Trypanosoma evansi, a disease found in horses, tested on 165 blood samples from Argentinean horses. The aim was to find the most effective techniques among the tested ones – Giemsa-stained smears (GSS), wet blood films (WBF), Strout’s concentration method (SCM), haematocrit centrifuge technique (HCT), buffy coat method (BCM), and mouse inoculation of blood (MBI).
Objective and Methodology
- The main objective of this study was to evaluate different parasitological diagnostic techniques, comparing their effectiveness in detecting equine trypanosomiasis caused by Trypanosoma evansi, a parasite infecting horses, especially in subtropical areas of Argentina.
- The six methods tested were Giemsa-stained smears (GSS), wet blood films (WBF), Strout’s concentration method (SCM), haematocrit centrifuge technique (HCT), buffy coat method (BCM), and mouse inoculation of blood (MBI).
- A total of 165 horse blood samples were collected from Formosa Province in Argentina and tested for the presence of Trypanosoma evansi using the six diagnostic methods.
Findings
- In the 165 tested blood samples, the parasite was detected in 52 samples (approximately 31.5%).
- Each method showed differing levels of sensitivity: mouse inoculation had the highest sensitivity at 88.2%, followed by the haematocrit centrifuge technique (HCT) at 71.1%, buffy coat method (BCM) at 63.4%, wet blood films (WBF) at 53.8%, Strout’s concentration method (SCM) at 46.1%, and finally Giemsa-stained Smears (GSS) at 45.6%.
Conclusion
- None of the single methods tested was found to be completely effective in diagnosing Trypanosoma evansi, meaning no individual technique could detect the parasite 100% of the time.
- Considering their respective sensitivity rates and overall effectiveness, the haematocrit centrifuge technique, mouse inoculation of blood, and Giemsa-stained smears are suggested to be the most effective diagnostic combination for Trypanosoma evansi.
- Using a combination of these methods may therefore provide more accurate and reliable results when diagnosing Trypanosoma evansi in horses.
Cite This Article
APA
Monzón CM, Mancebo OA, Roux JP.
(1990).
Comparison between six parasitological methods for diagnosis of Trypanosoma evansi in the subtropical area of Argentina.
Vet Parasitol, 36(1-2), 141-146.
https://doi.org/10.1016/0304-4017(90)90102-h Publication
Researcher Affiliations
- National Council for Scientific and Technical Research (CONICET), Argentina CEDIVEF, Formosa.
MeSH Terms
- Animals
- Argentina
- Biological Assay
- Hematocrit / veterinary
- Horse Diseases / blood
- Horse Diseases / diagnosis
- Horses
- Mice
- Predictive Value of Tests
- Rats
- Trypanosoma / isolation & purification
- Trypanosomiasis / blood
- Trypanosomiasis / diagnosis
- Trypanosomiasis / veterinary
Citations
This article has been cited 3 times.- Díaz AG, Ragone PG, Rusman F, Floridia-Yapur N, Barquez RM, Díaz MM, Tomasini N, Diosque P. A Novel Genotype and First Record of Trypanosoma lainsoni in Argentina.. Pathogens 2020 Sep 4;9(9).
- Li Z, Pinto Torres JE, Goossens J, Stijlemans B, Sterckx YG, Magez S. Development of a recombinase polymerase amplification lateral flow assay for the detection of active Trypanosoma evansi infections.. PLoS Negl Trop Dis 2020 Feb;14(2):e0008044.
- Birhanu H, Fikru R, Said M, Kidane W, Gebrehiwot T, Hagos A, Alemu T, Dawit T, Berkvens D, Goddeeris BM, Büscher P. Epidemiology of Trypanosoma evansi and Trypanosoma vivax in domestic animals from selected districts of Tigray and Afar regions, Northern Ethiopia.. Parasit Vectors 2015 Apr 9;8:212.
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