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Stem cells and development2018; 27(21); 1518-1525; doi: 10.1089/scd.2017.0241

Comparison of Antibacterial and Immunological Properties of Mesenchymal Stem/Stromal Cells from Equine Bone Marrow, Endometrium, and Adipose Tissue.

Abstract: Equine mesenchymal stem/stromal cells (MSCs) are multipotent cells that are widely used for treatment of musculoskeletal injuries, and there is significant interest in expanding their application to nonorthopedic conditions. MSCs possess antibacterial and immunomodulatory properties that may be relevant for combating infection; however, comparative studies using MSCs from different origins have not been carried out in the horse, and this was the focus of this study. Our results showed that MSC-conditioned media attenuated the growth of Escherichia coli, and that this effect was, on average, more pronounced for endometrium (EM)-derived and adipose tissue (AT)-derived MSCs than for bone marrow (BM)-derived MSCs. In addition, the antimicrobial lipocalin-2 was expressed at mean higher levels in EM-MSCs than in AT-MSCs and BM-MSCs, and the bacterial component lipopolysaccharide (LPS) stimulated its production by all three MSC types. We also showed that MSCs express interleukin-6 (IL-6), IL-8, monocyte chemoattractant protein-1, chemokine ligand-5, and Toll-like receptor 4, and that, in general, these cytokines were induced in all cell types by LPS. Low expression levels of the macrophage marker colony-stimulating factor 1 receptor were detected in BM-MSCs and EM-MSCs but not in AT-MSCs. Altogether, these findings suggest that equine MSCs from EM, AT, and BM have both direct and indirect antimicrobial properties that may vary between MSCs from different origins and could be exploited toward improvement of regenerative therapies for horses.
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Publication Date: 2018-09-06 PubMed ID: 30044182PubMed Central: PMC6209426DOI: 10.1089/scd.2017.0241Google Scholar: Lookup
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article focuses on the comparison of antibacterial and immunomodulatory properties of mesenchymal stem/stromal cells (MSCs) procured from different sources in horses. The paper discusses how these cells, sourced from endometrium (EM), bone marrow (BM), and adipose tissue (AT), can limit bacterial growth and influence responses of the immune system, thereby suggesting their potential usage in innovative therapies for horses.

Objective of the Research

  • The primary aim of the study was to compare and analyze the antibacterial and immunomodulatory features of MSCs derived from three diverse sources – equine bone marrow, endometrium, and adipose tissue.
  • The researchers strived to identify the source location that provides the most effective therapeutic properties for combating infections and tailoring improved regenerative therapies for horses.

Key Findings

  • The investigation found that conditioned media derived from the examined MSCs could curb the growth of Escherichia coli (E. coli). This inhibitory effect was observed more prominently with MSCs drawn from EM and AT, compared to BM.
  • The research identified higher levels of the antimicrobial lipocalin-2 protein in MSCs from EM compared to AT and BM. All three types of MSCs showed increased lipocalin-2 production when exposed to the bacterial component lipopolysaccharide (LPS).
  • The study revealed the presence of several cytokines in the MSCs, including IL-6, IL-8, monocyte chemoattractant protein-1, chemokine ligand-5, and Toll-like receptor 4. These cytokines were induced in all cell types by exposure to LPS.
  • Interestingly, the researchers discovered low expression levels of the macrophage marker, colony-stimulating factor 1 receptor, in MSCs obtained from BM and EM, but not in AT-sourced MSCs.

Conclusions

  • The study concludes by affirming the presence of direct and indirect antimicrobial properties in MSCs procured from EM, AT, and BM.
  • These properties varied depending on the source of MSCs, demonstrating the potential for tailored therapies. This information could prove essential for the development of innovative regenerative therapies for equine health.

Cite This Article

APA
Cortés-Araya Y, Amilon K, Rink BE, Black G, Lisowski Z, Donadeu FX, Esteves CL. (2018). Comparison of Antibacterial and Immunological Properties of Mesenchymal Stem/Stromal Cells from Equine Bone Marrow, Endometrium, and Adipose Tissue. Stem Cells Dev, 27(21), 1518-1525. https://doi.org/10.1089/scd.2017.0241

Publication

Stem cells and development
ISSN: 1557-8534
NlmUniqueID: 101197107
Country: United States
Language: English
Volume: 27
Issue: 21
Pages: 1518-1525

Researcher Affiliations

Cortés-Araya, Yennifer
  • 1 The Roslin Institute and R(D)SVS, University of Edinburgh , Edinburgh, United Kingdom .
Amilon, Karin
  • 1 The Roslin Institute and R(D)SVS, University of Edinburgh , Edinburgh, United Kingdom .
Rink, Burgunde Elisabeth
  • 1 The Roslin Institute and R(D)SVS, University of Edinburgh , Edinburgh, United Kingdom .
Black, Georgina
  • 1 The Roslin Institute and R(D)SVS, University of Edinburgh , Edinburgh, United Kingdom .
Lisowski, Zofia
  • 1 The Roslin Institute and R(D)SVS, University of Edinburgh , Edinburgh, United Kingdom .
Donadeu, Francesc Xavier
  • 1 The Roslin Institute and R(D)SVS, University of Edinburgh , Edinburgh, United Kingdom .
  • 2 The Euan Macdonald Centre for Motor Neurone Disease Research, University of Edinburgh , Edinburgh, United Kingdom .
Esteves, Cristina L
  • 1 The Roslin Institute and R(D)SVS, University of Edinburgh , Edinburgh, United Kingdom .

MeSH Terms

  • Adipose Tissue / cytology
  • Adipose Tissue / metabolism
  • Adipose Tissue / microbiology
  • Animals
  • Bone Marrow Cells / metabolism
  • Bone Marrow Cells / microbiology
  • Cell Differentiation / genetics
  • Cell Proliferation / genetics
  • Endometrium / growth & development
  • Endometrium / metabolism
  • Endometrium / microbiology
  • Escherichia coli / genetics
  • Escherichia coli / growth & development
  • Female
  • Gene Expression Regulation, Developmental
  • Horses / immunology
  • Horses / microbiology
  • Interleukin-6 / genetics
  • Interleukin-8 / genetics
  • Lipocalin-2 / genetics
  • Lipopolysaccharides
  • Macrophages / cytology
  • Macrophages / metabolism
  • Mesenchymal Stem Cells / immunology
  • Mesenchymal Stem Cells / metabolism
  • Mesenchymal Stem Cells / microbiology
  • Multipotent Stem Cells / cytology
  • Multipotent Stem Cells / metabolism
  • Multipotent Stem Cells / microbiology
  • Receptor, Macrophage Colony-Stimulating Factor / genetics
  • Toll-Like Receptor 4 / genetics

Grant Funding

  • Biotechnology and Biological Sciences Research Council

Conflict of Interest Statement

The authors declare no competing interests.

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