Comparison of cushioned centrifugation and SpermFilter filtration on longevity and morphology of cooled-stored equine semen.
Abstract: This study compares two methods for seminal plasma removal by evaluating sperm recovery rates, and motility and morphology of cooled-stored semen. Ejaculates were divided into three groups: control, filtration and cushioned centrifugation. Semen was extended to 25 million sperm/ml using a skim-milk-based extender and stored at 5°C for all groups. Sperm motility (total motility (%TM) and progressive motility (%PM)) was determined at 0, 24, 48 and 72 hours by a computer-assisted sperm analyser. Sperm morphology was assessed using differential interference microscopy. Overall, %TM of the centrifugation group was significantly higher than the filter group, but not significantly different than the control. No significant difference in %TM or %PM was detected for the control group and filter. Cushioned centrifugation was a superior method to obtain progressively motile sperm compared with control (P=0.03) and filter groups (P<0.001). No significant difference was found for the per cent of normal sperm cells and detached heads between the groups. This study demonstrated that cushioned centrifugation was a superior method to remove seminal plasma while preserving %TM and enhancing %PM for stallions under cooled storage over three days. However, as the differences appear to be negligible, the SpermFilter may represent an alternative for farms lacking a centrifuge.
British Veterinary Association.
Publication Date: 2016-02-23 PubMed ID: 26908160DOI: 10.1136/vr.103607Google Scholar: Lookup
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- Comparative Study
- Journal Article
Summary
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The study focuses on comparing the efficiency of two methods, cushioned centrifugation and SpermFilter filtration, used in the removal of seminal plasma, considering the effects these methods have on the life-span, and metamorphosis of cooled-preserved equine sperm.
Research Context
- The study stems from the importance of removing seminal plasma to maintain the longevity and quality of sperm during storage and transport in reproductive management.
- Two techniques, cushioned centrifugation and SpermFilter filtration, are compared in terms of their efficiency in seminal plasma removal and their impact on the morphology and longevity of cooled-stored equine semen.
Approach of the Research
- The ejaculates used in the study were separated into three groups: a control group without any treatment, a filtration group employing the SpermFilter filtration technique, and a cushioned centrifugation group.
- Using a skim-milk-based extender, the semen was diluted to a concentration of 25 million sperm per milliliter, and then stored at 5°C across all three groups.
Analysis Methods
- Researchers used computer-assisted sperm analysis to calculate sperm motility, including total and progressive motility, at 4 different intervals: 0, 24, 48, and 72 hours.
- With the help of differential interference microscopy, sperm morphology (structure) was examined and assessed.
Key Findings
- The results suggested that the centrifugation group displayed a significantly higher total motility percentage compared to the filter group, and there was no significant disparity compared to the control group.
- No significant alterations were detected in the total or progressive motility percentages between the filter and control groups.
- The cushioned centrifugation method was outperforming both the control group and filter groups when considering the acquisition of progressively motile sperm.
- In terms of normal sperm cells and number of detached heads per group, no meaningful difference was detected.
Implications of the Study
- Overall, the results indicate that cushioned centrifugation emerges as a superior method for seminal plasma removal while preserving total motility and enhancing progressive motility of stallion semen in cool storage conditions over a span of three days.
- On the other hand, considering the minor differences in the two techniques, SpermFilter filtration could be a practical alternative for facilities that do not have access to a centrifuge.
Cite This Article
APA
Roach J, Schnobrich M, Ellerbrock R, Feijo L, Bradecamp E, Alvarenga MA, Kline K, Canisso I.
(2016).
Comparison of cushioned centrifugation and SpermFilter filtration on longevity and morphology of cooled-stored equine semen.
Vet Rec, 178(10), 241.
https://doi.org/10.1136/vr.103607 Publication
Researcher Affiliations
- Rood and Riddle Equine Hospital, 2150 Georgetown Road, Lexington, KY 40511, USA.
- Rood and Riddle Equine Hospital, 2150 Georgetown Road, Lexington, KY 40511, USA.
- Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana-Champaign, 1008 West Hazelwood Drive, Urbana, IL 61802, USA.
- Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana-Champaign, 1008 West Hazelwood Drive, Urbana, IL 61802, USA.
- Rood and Riddle Equine Hospital, 2150 Georgetown Road, Lexington, KY 40511, USA.
- Departmento de Reprodução Animal e Radiologia, Faculdade de Medicina Veterinaria e Zootecnia, Universidade Estadual Paulista, Rubião Junior, Botucatu, São Paulo, Brazil.
- Department of Animal Sciences, University of Illinois Urbana-Champaign, 1207 W Gregory Drive, Urbana, IL 61801, USA.
- Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana-Champaign, 1008 West Hazelwood Drive, Urbana, IL 61802, USA.
MeSH Terms
- Animals
- Centrifugation / methods
- Centrifugation / veterinary
- Cryopreservation / veterinary
- Filtration / methods
- Filtration / veterinary
- Horses
- Longevity
- Male
- Semen Preservation / methods
- Semen Preservation / veterinary
- Sperm Motility / physiology
- Spermatozoa / cytology
- Spermatozoa / physiology
Citations
This article has been cited 3 times.- Podico G, Spencer KM, Magalhaes HB, Canisso IF. Semen Quality of the First and Second Ejaculates Collected from Breeding Inactive Stallions after Cooling and Freezing. Vet Sci 2023 Feb 21;10(3).
- Falchi L, Khalil WA, Hassan M, Marei WFA. Perspectives of nanotechnology in male fertility and sperm function. Int J Vet Sci Med 2018 Dec;6(2):265-269.
- Zabala SM, Serres C, Montero N, Crespo F, Lorenzo PL, Pérez-Aguilera V, Galán C, Domínguez-Gimbernat M, Oliet A, Moreno S, González-Zorn B, Gutiérrez-Cepeda L. Strategies to Reduce the Use of Antibiotics in Fresh and Chilled Equine Semen. Animals (Basel) 2024 Jan 5;14(2).
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