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Andrologia2020; 52(4); e13545; doi: 10.1111/and.13545

Comparison of different mathematical models to assess seasonal variations in the longevity of DNA integrity of cooled-stored stallion sperm.

Abstract: Dynamic assessment of sperm DNA fragmentation (SDF) has shown to give fuller understanding of stallion semen quality; however, there have been limited attempts to use this parameter to investigate seasonal changes in productive functions. The aims of this study were to: (a) establish a reliable mathematical model to describe the longevity of cooled-stored sperm DNA integrity; (b) to examine the effect of seasonal variations on SDF. Ejaculates were cooled to 5°C, and SDF was analysed after 0, 6 and 24 hr of storage. The coefficient of determination (R ) was calculated after fine-tuning linear (LIN), exponential (EXP) and second order polynomial (POL) models. R was significantly higher (p < .001) for POL than for LIN and EXP. The rate of DNA degradation was calculated using the slopes of POL equations. After assessing the rate of change of the POL functions, significant differences between the acceleration of DNA fragmentation were found (p < .01) among seasons, being higher for winter and summer than spring and autumn. In conclusion, DNA analysis of stallion sperm fits better to a second order polynomial mathematical model, being spring the best season to collect and process cooled stallion semen in order to maintain the DNA integrity of the stallion sperm.
© 2020 Blackwell Verlag GmbH.
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Publication Date: 2020-02-28 PubMed ID: 32109320DOI: 10.1111/and.13545Google Scholar: Lookup
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  • Comparative Study
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research uses math models to consider how the DNA longevity of cooled-stored stallion sperm changes across the seasons, finding that spring is the best season for maintaining DNA integrity of the stallion sperm.

Objective of the Study

  • Primarily, this study aims to establish a dependable mathematical model that can accurately illustrate the longevity of DNA integrity in cooled stallion sperm.
  • Secondly, the study seeks to investigate the impact of seasonal variations on sperm DNA fragmentation (SDF), a crucial parameter linked to semen quality.

Methods and Models Used

  • The authors used ejaculates that were cooled to 5°C and analyzed the SDF after 0, 6, and 24 hours of storage.
  • Three mathematical models were utilized and fine-tuned to achieve this: linear (LIN), exponential (EXP), and second order polynomial (POL).
  • The effectiveness of these models was measured using the coefficient of determination (R), whose value reveals how closely the data follows the model’s line of best fit.

Findings of the Study

  • The researchers discovered that the POL model had a significantly higher R-value, suggesting that it more accurately represents the data on stallion sperm DNA integrity over time compared to the LIN and EXP models.
  • Using the slope of the POL equations, they calculated the rate of DNA degradation for the sperm samples.
  • Significant differences in the acceleration of DNA fragmentation were found among the seasons. DNA fragmentation was higher in winter and summer compared to spring and autumn.

Conclusion

  • Based on the study findings, the second order polynomial mathematical model was determined to be the most suitable for illustrating DNA longevity in cooled-stored stallion sperm.
  • Spring emerged as the best season for collecting and processing cooled stallion semen to keep the DNA integrity of the sperm intact.

Cite This Article

APA
Ortiz I, Quiñones-Pérez C, Hidalgo M, Consuegra C, Diaz-Jimenez M, Dorado J, Vega-Pla JL, Crespo F. (2020). Comparison of different mathematical models to assess seasonal variations in the longevity of DNA integrity of cooled-stored stallion sperm. Andrologia, 52(4), e13545. https://doi.org/10.1111/and.13545

Publication

Andrologia
ISSN: 1439-0272
NlmUniqueID: 0423506
Country: Germany
Language: English
Volume: 52
Issue: 4
Pages: e13545

Researcher Affiliations

Ortiz, Isabel
  • College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX, USA.
Quiñones-Pérez, Carlota
  • Centro Militar de Cría Caballar de Ávila, Cría Caballar de las Fuerzas Armadas, Ávila, Spain.
  • Laboratorio de Investigación Aplicada, Cría Caballar de las Fuerzas Armadas, Córdoba, Spain.
Hidalgo, Manuel
  • Veterinary Reproduction Group, Department of Animal Medicine and Surgery, University of Cordoba, Cordoba, Spain.
Consuegra, Cesar
  • Veterinary Reproduction Group, Department of Animal Medicine and Surgery, University of Cordoba, Cordoba, Spain.
Diaz-Jimenez, Maria
  • Veterinary Reproduction Group, Department of Animal Medicine and Surgery, University of Cordoba, Cordoba, Spain.
Dorado, Jesus
  • Veterinary Reproduction Group, Department of Animal Medicine and Surgery, University of Cordoba, Cordoba, Spain.
Vega-Pla, Jose Luis
  • Laboratorio de Investigación Aplicada, Cría Caballar de las Fuerzas Armadas, Córdoba, Spain.
Crespo, Francisco
  • Centro Militar de Cría Caballar de Ávila, Cría Caballar de las Fuerzas Armadas, Ávila, Spain.

MeSH Terms

  • Animals
  • DNA Fragmentation
  • Horses
  • Male
  • Models, Statistical
  • Spermatozoa
  • Tissue Preservation

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Citations

This article has been cited 1 times.
  1. Dziekońska A, Szczepańska A, Wysokińska A. Effect of Season on the Characteristics of Warmblood Stallion Spermatozoa Stored in a Liquid State at 5 °C. Animals (Basel) 2025 Apr 3;15(7).
    doi: 10.3390/ani15071035pubmed: 40218428google scholar: lookup
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