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Equine veterinary journal. Supplement1998; (25); 47-50; doi: 10.1111/j.2042-3306.1997.tb05099.x

Comparison of different methods for the recovery of horse oocytes.

Abstract: The object of this study was to compare 4 different methods of oocyte recovery from mares; 1) transvaginal follicle aspiration in vivo; 2) follicle aspiration in vitro; 3) oocyte recovery by isolation of follicles in vitro and 4) follicle scraping in vitro. Oocyte recovery was highest after follicle scraping (71.1%) and follicle isolation and rupture (61.3%). Follicle aspiration in vitro and in vivo yielded oocytes on 31.2% and 19.3% of occasions, respectively. The output of different types of cumulus-oocyte-complexes was different among the methods; the portion of compact cumulus-oocyte-complexes was significantly higher with follicle scraping (50.7%) and follicle isolation (44.5%) than with aspiration in vivo (31.9%) and in vitro (23.7%). The recovery rate of oocytes from small follicles (<15 mm) was significantly higher than from larger follicles (P<0.05) using transvaginal follicle aspiration. The proportion of oocytes that were degenerate (exhibited shrunken, dense or visibly damaged ooplasm) ranged from 1.2% after follicle scraping, to 17.2% after aspiration in vivo. These results indicate that, for the recovery of horse oocytes in vitro, follicle scraping and follicle isolation give the highest recoveries of cumulus-intact oocytes.
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Publication Date: 1998-05-21 PubMed ID: 9593527DOI: 10.1111/j.2042-3306.1997.tb05099.xGoogle Scholar: Lookup
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  • Comparative Study
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article investigates the efficacy of four different methods for recovering horse oocytes, with follicle scraping and follicle isolation producing the highest recovery rates.

Methodology and Study Groups

  • The researchers compared four distinct techniques for recovering horse oocytes (mature egg cells) from mares.
  • These methods included: transvaginal follicle aspiration in vivo (within the living horse), follicle aspiration in vitro (outside the living horse), oocyte recovery via isolation of follicles in vitro, and follicle scraping in vitro.

Results and Insights

  • The study found that follicle scraping yielded the highest recovery rate of oocytes at 71.1%. The recovery by isolation of follicles resulted in a slightly lower rate of 61.3%.
  • In contrast, the more traditional methods of follicle aspiration in vivo and in vitro only resulted in successful oocyte recoveries 19.3% and 31.2% of the time respectively.
  • Notably, the type of cumulus-oocyte-complexes (COCs) – the structure comprised of a germ cell surrounded by granulosa cells – differed among the various methods. Methods yielding a compact COC were higher with follicle scraping (50.7%) and follicle isolation (44.5%) in comparison to aspiration in vivo (31.9%) and in vitro (23.7%).

Additional Findings

  • Another important observation noted was that the recovery rate of oocytes from small follicles (less than 15mm) was significantly higher when using transvaginal follicle aspiration.
  • The researchers also noted the proportion of oocytes that were degenerate after the process – these had a shrunken, dense or visibly damaged ooplasm (the cytoplasm of a mature egg). This ranged from as low as 1.2% after follicle scraping to as high as 17.2% after aspiration in vivo.

Conclusions

  • In summary, the research suggests that follicle scraping and isolation are the most effective techniques for the recovery of horse oocytes.
  • It not only showed higher recovery rates but also exhibited higher incidences of intact COCs and lower percentage of degenerate oocytes.
  • These findings are important for techniques such as in vitro fertilization where the successful recovery of healthy oocytes is crucial.

Cite This Article

APA
Alm H, Torner H, Kanitz W, Becker F, Hinrichs K. (1998). Comparison of different methods for the recovery of horse oocytes. Equine Vet J Suppl(25), 47-50. https://doi.org/10.1111/j.2042-3306.1997.tb05099.x

Publication

Equine veterinary journal. Supplement
NlmUniqueID: 9614088
Country: United States
Language: English
Issue: 25
Pages: 47-50

Researcher Affiliations

Alm, H
  • Research Institute for the Biology of Farm Animals, Dummerstorf, Germany.
Torner, H
    Kanitz, W
      Becker, F
        Hinrichs, K

          MeSH Terms

          • Animals
          • Female
          • Fertilization in Vitro / methods
          • Fertilization in Vitro / veterinary
          • Horses / physiology
          • Oocytes / cytology
          • Ovarian Follicle / cytology
          • Suction / veterinary

          Citations

          This article has been cited 6 times.
          1. Márquez-Moya A, Carreras-Vico N, Sala-Ayala L, Martínez-Boví R, Cuervo-Arango J. Comparative Evaluation of Follicular Flushing Frequency and Scraping Time During Ovum Pick-Up in Mares: Effects on Oocyte Recovery Rate and Technical Considerations. Reprod Domest Anim 2026 Feb;61(2):e70183.
            doi: 10.1111/rda.70183pubmed: 41674388google scholar: lookup
          2. Huijsmans TERG, Hassan HA, Smits K, Van Soom A. Postmortem Collection of Gametes for the Conservation of Endangered Mammals: A Review of the Current State-of-the-Art. Animals (Basel) 2023 Apr 15;13(8).
            doi: 10.3390/ani13081360pubmed: 37106923google scholar: lookup
          3. Kaps M, Okada CTC, Gautier CM, Aurich J, Aurich C. Deslorelin Slow-Release Implants Delay Ovulation and Increase Plasma AMH Concentration and Small Antral Follicles in Haflinger Mares. Animals (Basel) 2021 May 28;11(6).
            doi: 10.3390/ani11061600pubmed: 34071625google scholar: lookup
          4. Abdoon AS, Abdel-Rahman HA, Shawki SM, Kandil OM, Fathalla SI. Influence of follicle size, methods of retrieval on oocytes yield and morphology in Egyptian Jennies ovaries with special reference to maturation rate in vitro. Vet Res Commun 2014 Dec;38(4):287-95.
            doi: 10.1007/s11259-014-9617-ypubmed: 25224754google scholar: lookup
          5. Abdel-Khalek EA, El-Harairy MA, Shamiah ShM, Khalil WA. Effect of ovary preservation period on recovery rate and categories of dromedary camel oocytes. Saudi J Biol Sci 2010 Jul;17(3):231-5.
            doi: 10.1016/j.sjbs.2010.04.007pubmed: 23961083google scholar: lookup
          6. Cremonesi F, Anderson K, Lange-Consiglio A. Efficacy of tuohy needle in oocytes collection from excised mare ovaries. Vet Med Int 2010 Aug 5;2010.
            doi: 10.4061/2010/102591pubmed: 20814438google scholar: lookup
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