Comparison of platelet counting technologies in equine platelet concentrates.
Abstract: (1) To compare the performance of 4 platelet counting technologies in equine platelet concentrates and (2) to evaluate the ability of the Magellan platelet rich plasma (PRP) system to concentrate equine platelets. Methods: Experimental study to assess method agreement. Methods: Adult mixed breed horses (n = 32). Methods: Acid citrate dextrose-A anti-coagulated whole blood was collected and PRP produced using the Magellan system according to the manufacturer's instructions. Platelets were quantified using 4 counting methods: optical scatter (Advia 2120), impedance (CellDyn 3700), hand counting, and fluorescent antibody flow cytometry. Platelet concentrations were compared using Passing and Bablok regression analyses and mixed model ANOVA. Significance was set at P < .05. Results: Platelet concentrations measured in identical PRP samples were consistently higher for the Advia 2120 than the CellDyn 3700. Systematic and proportional biases were observed between these 2 automated methods when analyzed by regression analysis of the larger sample size. No bias (systematic or proportional) was observed among any of the other counting methods. Despite the bias detected between the 2 automated systems, there were no significant differences on average among the 4 counting methods evaluated, based on the ANOVA. The Magellan system consistently generated high platelet concentrations as well as higher than expected WBC concentrations. Conclusions: The Magellan system delivered desirably high platelet concentrations; however, WBC concentrations may be unacceptably high for some orthopedic applications. All 4 platelet counting methods tested were equivalent on average and therefore suitable for quantifying platelets in equine PRP used for clinical applications.
© Copyright 2014 by The American College of Veterinary Surgeons.
Publication Date: 2014-10-13 PubMed ID: 25307726DOI: 10.1111/j.1532-950X.2014.12290.xGoogle Scholar: Lookup
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- Comparative Study
- Evaluation Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research focused on comparing four platelet counting technologies when dealing with horse platelet concentrates, and also assessed the efficacy of the Magellan platelet rich plasma (PRP) system at concentrating horse platelets. Results indicated the Magellan system was generally successful, though it had higher than anticipated white blood cell concentrations.
Research Methods
- Blood was collected from adult mixed breed horses (32 in total) to create the platelet-rich plasma. Acid citrate dextrose-A was used as an anti-coagulant and the Magellan system created the PRP according to the manufacturer’s specified procedures.
- Four counting methods were used to quantify the platelets present in the PRP: optical scatter (Advia 2120), impedance (CellDyn 3700), hand counting, and fluorescent antibody flow cytometry.
- Platelet concentrations determined via these methods were then compared using Passing and Bablok regression analyses as well as mixed model ANOVA, with significance being set at P < .05.
Findings
- The Advia 2120 consistently registered higher platelet counts as compared to CellDyn 3700 when analysing identical PRP samples.
- A systematic and proportional bias was noted between these two automated methods when regression analysis was carried out on a larger sample size.
- Neither systematic nor proportional bias was detected among any of the other platelet counting methods.
- Even with the bias found between the two automated systems, there were no significant differences on average among the four counting methods evaluated, as deduced by the ANOVA.
- The Magellan system regularly yielded high platelet concentrations but also exhibited higher than expected white blood cell concentrations.
Conclusion
- The research concluded that the Magellan system was effective in generating desirable concentrations of platelets, but cautioned that the white blood cell concentrations might be excessively high for some orthopaic applications.
- All four platelet counting methods were declared equivalent on average and hence suitable for quantifying platelets in equine PRP for clinical applications.
Cite This Article
APA
O'Shea CM, Werre SR, Dahlgren LA.
(2014).
Comparison of platelet counting technologies in equine platelet concentrates.
Vet Surg, 44(3), 304-313.
https://doi.org/10.1111/j.1532-950X.2014.12290.x Publication
Researcher Affiliations
- Department of Large Animal Clinical Sciences, Virginia-Maryland College of Veterinary Medicine, Virginia Tech, Blacksburg, Virginia.
MeSH Terms
- Animals
- Blood Platelets / physiology
- Citric Acid
- Glucose / analogs & derivatives
- Horses / blood
- Horses / physiology
- Platelet Count / instrumentation
- Platelet Count / veterinary
- Platelet-Rich Plasma / cytology
- Predictive Value of Tests
- Regression Analysis
Citations
This article has been cited 7 times.- Carmona JU, López C, Argüelles D. Addressing Heterogeneity in Equine PRP Therapies: A Scoping Review of Methods, Evidence, and Commercial Validation. Animals (Basel) 2025 Dec 13;15(24).
- Seidel SRT, de Souza AF, Fülber J, Bogossian PM, Rodrigues NNP, Baccarin RYA. Evaluation of platelet-rich plasma applied in the coronary band of healthy equine hooves. Can Vet J 2021 Jul;62(7):729-735.
- Segabinazzi LGTM, Podico G, Rosser MF, Nanjappa SG, Alvarenga MA, Canisso IF. Three Manual Noncommercial Methods to Prepare Equine Platelet-Rich Plasma. Animals (Basel) 2021 May 21;11(6).
- Segabinazzi LGTM, Canisso IF, Podico G, Cunha LL, Novello G, Rosser MF, Loux SC, Lima FS, Alvarenga MA. Intrauterine Blood Plasma Platelet-Therapy Mitigates Persistent Breeding-Induced Endometritis, Reduces Uterine Infections, and Improves Embryo Recovery in Mares. Antibiotics (Basel) 2021 Apr 23;10(5).
- McClain AK, McCarrel TM. The effect of four different freezing conditions and time in frozen storage on the concentration of commonly measured growth factors and enzymes in equine platelet-rich plasma over six months. BMC Vet Res 2019 Aug 14;15(1):292.
- Sun Y, Hu Z, Huang Z, Chen H, Qin S, Jianing Z, Chen S, Qin X, Ye Y, Wang C. Compare the accuracy and precision of Coulter LH780, Mindray BC-6000 Plus, and Sysmex XN-9000 with the international reference flow cytometric method in platelet counting. PLoS One 2019;14(5):e0217298.
- Bogers SH. Cell-Based Therapies for Joint Disease in Veterinary Medicine: What We Have Learned and What We Need to Know. Front Vet Sci 2018;5:70.
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