Comparison of Tenocyte Populations from the Core and Periphery of Equine Tendons.
Abstract: Tendon is a highly organized, dense connective tissue that has been demonstrated to have very little turnover. In spite of the low turnover, tendon can grow in response to loading, which may take place primarily at the periphery. Tendon injuries and recurrence of injuries are common in both humans and animals in sports. It is unclear why some areas of the tendon are more susceptible to such injuries and whether this is due to intrinsic regional differences in extracellular matrix (ECM) production or tissue turnover. This study aimed to compare populations of tenocytes derived from the tendon core and periphery. Tenocytes were isolated from equine superficial digital flexor tendons (SDFTs), and the proliferation capacity was determined. ECM production was characterized by immuno- and histological staining and by liquid chromatography-mass spectrometry-based proteomics. Core and periphery SDFT cultures exhibited comparable proliferation rates and had very similar proteome profiles, but showed biological variation in collagen type I deposition. In conclusion, the intrinsic properties of tenocytes from different regions of the tendon are very similar, and other factors in the tissue may contribute to how specific areas respond to loading or injury.
Publication Date: 2020-09-02 PubMed ID: 32822197DOI: 10.1021/acs.jproteome.0c00591Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article discusses a study comparing the tenocyte populations in both core and periphery of equine tendons. The study aims to understand why certain areas of the tendon are more prone to injuries by investigating whether there are inherent differences in each region’s extracellular matrix production or tissue renewal rate.
Research Objective
- The aim of this research is to investigate and contrast the populations of tenocytes (tendon cells) in both core and periphery regions of equine tendons. The objective is to understand the possible susceptibility of these areas to injuries, which is quite prevalent in sports activities related to both humans and animals.
Methodology
- Tenocytes were isolated from equine superficial digital flexor tendons (SDFTs).
- The replication capacity of the tenocytes was then determined.
- Extracellular Matrix (ECM) production was characterized using immunological and histological staining in addition to proteomics analysis based on liquid chromatography-mass spectrometry.
Results
- The study found that tenocytes cultures from both core and periphery SDFT showed similar proliferation rates and comparable proteome profiles.
- However, it was observed that there were biological differences in the deposition of collagen type I across the different cultures.
Conclusion
- The study concludes that the intrinsic properties and characteristics of tenocytes from different regions of the tendon are very alike.
- It suggests that other factors present in the tissue could be contributing to how specific areas respond to loading or get inflicted by injury.
Cite This Article
APA
Zhang C, Svensson RB, Montagna C, Carstensen H, Buhl R, Schoof EM, Kjaer M, Magnusson SP, Yeung CC.
(2020).
Comparison of Tenocyte Populations from the Core and Periphery of Equine Tendons.
J Proteome Res, 19(10), 4137-4144.
https://doi.org/10.1021/acs.jproteome.0c00591 Publication
Researcher Affiliations
- Institute of Sports Medicine Copenhagen, Bispebjerg Hospital and Center for Healthy Aging, University of Copenhagen, 2400 Copenhagen, Denmark.
- Institute of Sports Medicine Copenhagen, Bispebjerg Hospital and Center for Healthy Aging, University of Copenhagen, 2400 Copenhagen, Denmark.
- Institute of Sports Medicine Copenhagen, Bispebjerg Hospital and Center for Healthy Aging, University of Copenhagen, 2400 Copenhagen, Denmark.
- Department of Veterinary Clinical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 2630 Taastrup, Denmark.
- Department of Veterinary Clinical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 2630 Taastrup, Denmark.
- Proteomics Core, Technical University of Denmark, 2800 Kongens Lyngby, Denmark.
- Institute of Sports Medicine Copenhagen, Bispebjerg Hospital and Center for Healthy Aging, University of Copenhagen, 2400 Copenhagen, Denmark.
- Institute of Sports Medicine Copenhagen, Bispebjerg Hospital and Center for Healthy Aging, University of Copenhagen, 2400 Copenhagen, Denmark.
- Department of Physical and Occupational Therapy, Bispebjerg Hospital, 2400 Copenhagen, Denmark.
- Institute of Sports Medicine Copenhagen, Bispebjerg Hospital and Center for Healthy Aging, University of Copenhagen, 2400 Copenhagen, Denmark.
MeSH Terms
- Animals
- Extracellular Matrix
- Horses
- Humans
- Proteomics
- Tendon Injuries
- Tendons
- Tenocytes
Citations
This article has been cited 4 times.- Sarmiento P, Little D. Tendon and multiomics: advantages, advances, and opportunities. NPJ Regen Med 2021 Oct 1;6(1):61.
- Alkhadidi F, AlSharif H, AlQthami A, Alkhaldi SH, Alsuwat SA, Abosabie SA, Abosabie SA, Kamal NM. Novel homozygous C3orf67 gene variant associated with primary ciliary dyskinesia in a Saudi pediatric patient: A case report. World J Exp Med 2025 Dec 20;15(4):108404.
- Djalali-Cuevas A, Rettel M, Stein F, Savitski M, Kearns S, Kelly J, Biggs M, Skoufos I, Tzora A, Prassinos N, Diakakis N, Zeugolis DI. Macromolecular crowding in human tenocyte and skin fibroblast cultures: A comparative analysis. Mater Today Bio 2024 Apr;25:100977.
- Data K, Kulus M, Ziemak H, Chwarzyński M, Piotrowska-Kempisty H, Bukowska D, Antosik P, Mozdziak P, Kempisty B. Decellularization of Dense Regular Connective Tissue-Cellular and Molecular Modification with Applications in Regenerative Medicine. Cells 2023 Sep 16;12(18).
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