Comparison of the antigenic relationship between Japanese encephalitis virus genotypes 1 and 3.
Abstract: The Japanese encephalitis virus (JEV) genotype circulating in Korea has changed from G3 to G1. Therefore, the purpose of this study was to compare the antigenic relationship between the two genotypes by using antibody tests. Methods: Blood samples from 42 sows and 216 horses were collected, and their seroprevalence was monitored using the hemagglutination inhibition and virus neutralization tests. Antisera against JEV G1 and G3 were isolated and prepared from guinea pigs. The cross-reactivity of these two viruses was then compared using the neutralizing antibody test. Results: We found that there was a difference in the seropositive ratios of JEV G1 and G3. However, the difference was dependent on the antibody test used. There was also an observed difference in the antigenicity between the two genotypes, as ascertained using the neutralizing antibody test. Conclusions: There is an evident difference in JEV antigenicity between the genotypes G1 and G3. Therefore, we propose monitoring of the seroprevalence of JEV, and reevaluating the antigenicity of the current vaccine by using the relevant tests.
Publication Date: 2016-01-27 PubMed ID: 26866021PubMed Central: PMC4742595DOI: 10.7774/cevr.2016.5.1.26Google Scholar: Lookup
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- Journal Article
Summary
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This research focuses on the antigenic relationship between two genotypes (G1 and G3) of the Japanese encephalitis virus (JEV) prevalent in Korea, in efforts to improve vaccine efficacy due to changes in circulating genotypes.
Research Methodology
- Researchers collected blood samples from 42 sows and 216 horses for this study.
- Two antibody tests, the hemagglutination inhibition and virus neutralization tests, were used to monitor the prevalence of the virus (seroprevalence) in the samples.
- The team prepared antisera against both genotypes, G1 and G3, of the Japanese encephalitis virus using guinea pigs. The antisera are blood serums that contain antibodies against these specific genotypes.
- Finally, they evaluated the cross-reactivity of the two viruses by comparing how each antivirus reacted to both genotypes.
Key Findings
- The research revealed that individual seropositive ratios, a measure of the prevalence of the virus, varied between JEV G1 and G3. This dependency was also reliant on the type of antibody test used.
- The neutralizing antibody test highlighted a difference in the antigenicity (ability to provoke an immune response) between the two genotypes. This difference implies that the viruses have varied capacities to stimulate an immune response.
Conclusions and Recommendations
- The researchers concluded that there’s a distinguishable difference in how the two JEV genotypes, G1 and G3, stimulate an immune response.
- They proposed further consistent monitoring of the prevalence of different JEV genotypes.
- Additionally, reevaluation of the current vaccine’s effectiveness regarding these antigenic differences is advised to optimize its protective efficacy against newer circulating genotypes.
Cite This Article
APA
Kang BK, Hwang JM, Moon H, Han SY, Kim JM, Yang DK, Park BK, Song D.
(2016).
Comparison of the antigenic relationship between Japanese encephalitis virus genotypes 1 and 3.
Clin Exp Vaccine Res, 5(1), 26-30.
https://doi.org/10.7774/cevr.2016.5.1.26 Publication
Researcher Affiliations
- Research Unit, Green Cross Veterinary Products, Yongin, Korea.
- Research Unit, Green Cross Veterinary Products, Yongin, Korea.
- Research Unit, Green Cross Veterinary Products, Yongin, Korea.
- Research Unit, Green Cross Veterinary Products, Yongin, Korea.
- Research Unit, Green Cross Veterinary Products, Yongin, Korea.
- Animal and Plant Quarantine Agency, Anyang, Korea.
- Department of Veterinary Medicine Virology Laboratory, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Korea.
- College of Pharmacy, Korea University, Sejong, Korea.
Conflict of Interest Statement
No potential conflict of interest relevant to this article was reported.
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Citations
This article has been cited 6 times.- Mackenzie JS, Williams DT, van den Hurk AF, Smith DW, Currie BJ. Japanese Encephalitis Virus: The Emergence of Genotype IV in Australia and Its Potential Endemicity. Viruses 2022 Nov 9;14(11).
- Mulvey P, Duong V, Boyer S, Burgess G, Williams DT, Dussart P, Horwood PF. The Ecology and Evolution of Japanese Encephalitis Virus. Pathogens 2021 Nov 24;10(12).
- Kim HH, Yang DK, Ji M, Lee SK, Hyun BH. Genotype-specific neutralizing antibody titers against Japanese encephalitis virus genotypes 1 and 3 in horses immunized with a genotype 3 vaccine. Clin Exp Vaccine Res 2020 Jul;9(2):102-107.
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