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Home / Equine Research Bank / Reprod Biol Endocrinol / Comparison of the biopsy and cytobrush techniques for diagno...
Reproductive biology and endocrinology : RB&E2014; 12; 27; doi: 10.1186/1477-7827-12-27

Comparison of the biopsy and cytobrush techniques for diagnosis of subclinical endometritis in mares.

Abstract: Endometritis is a major cause of infertility in the mare. Therefore, the diagnosis of this disease is very important in veterinary practice. The objective of this study was to compare bacteriological and cytological results obtained from the mare uterus using biopsy (EB) and cytobrush (CB) techniques and relating these findings to the presence of polymorphonuclear cells (PMNs) in endometrial tissue as the gold standard for detection of endometritis. In particular, we tested the hypothesis that endometrial cytology and microbiology data obtained from material collected using the EB and CB techniques are similar, so that the CB technique could preferentially be used to detect subclinical endometritis in clinical practice. Methods: A total of 69 mares suspected of subclinical endometritis because of previous reproductive history and 15 maiden mares were enrolled in this study. Material collected from both EB and CB was smeared on sterile glass slides for cytological examinations and on culture media for microbiological examinations. Bacteriological cultures and cytological samples were classified as negative (no growth or mixed cultures of more than three microorganisms; <2% PMNs) or positive (pure growth of microorganisms; >2% PMNs) for endometritis. Results: Positive growth was observed in 43% of CB samples and in 54% of EB samples (difference not significant). The growth of β-hemolytic streptococci was always connected with positive cytology. This relationship was not observed for growth of E. coli or for non-pathogenic flora. The sensitivity of bacterial growth and cytology from EB was 0.63 and 0.73 respectively. The sensitivities of bacterial growth and cytology from CB were 0.50 and 0.71 respectively. Conclusions: Microbiological and cytological results obtained from CB are similar to those obtained from EB and based on these findings the CB technique may be recommended for collection of materials from the mare's uterus in clinical practice.
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Publication Date: 2014-04-04 PubMed ID: 24708825PubMed Central: PMC3986439DOI: 10.1186/1477-7827-12-27Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

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This research article investigates the effectiveness of two methods, endometrial biopsy (EB) and cytobrush (CB), in diagnosing subclinical endometritis in mares. It concludes that the CB technique is as effective as the EB method and could be used as a primary detection tool for subclinical endometritis in clinical practice.

Research Aims and Objectives

  • The study aims to compare the bacteriological and cytological results obtained from the uterus of mares using biopsy (EB) and cytobrush (CB) techniques.
  • The research specifically tests the hypothesis that data derived from materials collected through the EB and CB techniques are similar and thus, in clinical practice, the CB technique could be preferable to detect subclinical endometritis.

Methodology

  • The researchers enrolled 69 mares suspected of having subclinical endometritis, owing to their reproductive history, and 15 maiden mares for this study.
  • Material was gathered from both EB and CB methods and smeared on sterile glass slides for cytological examinations and on culture media for microbiological examinations.
  • Bacteriological cultures and cytological samples were classified as either negative or positive for endometritis.

Results

  • The study observed a positive growth in 43% of CB samples and in 54% of EB samples (a non-significant difference).
  • The presence of β-hemolytic streptococci was always associated with positive cytology. This association was not noted for growth of E. coli or for non-pathogenic flora.
  • The sensitivity of bacterial growth and cytology from EB was 0.63 and 0.73 respectively.
  • The sensitivity of bacterial growth and cytology from CB were 0.50 and 0.71 respectively.

Conclusions

  • The analysis shows similar microbiological and cytological results from CB and EB techniques.
  • Based on these findings, the study concludes that the CB technique can be utilized in clinical practice for sample collection from the mare’s uterus, as it provides results comparable to the EB technique.

Cite This Article

APA
Buczkowska J, Kozdrowski R, Nowak M, Raś A, Staroniewicz Z, Siemieniuch MJ. (2014). Comparison of the biopsy and cytobrush techniques for diagnosis of subclinical endometritis in mares. Reprod Biol Endocrinol, 12, 27. https://doi.org/10.1186/1477-7827-12-27

Publication

Reproductive biology and endocrinology : RB&E
ISSN: 1477-7827
NlmUniqueID: 101153627
Country: England
Language: English
Volume: 12
Pages: 27

Researcher Affiliations

Buczkowska, Justyna
    Kozdrowski, Roland
    • Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Wrocław, Poland. roland.kozdrowski@up.wroc.pl.
    Nowak, Marcin
      Raś, Andrzej
        Staroniewicz, Zdzisław
          Siemieniuch, Marta J

            MeSH Terms

            • Animals
            • Biopsy / methods
            • Biopsy / standards
            • Cytological Techniques / methods
            • Cytological Techniques / standards
            • Endometritis / pathology
            • Female
            • Horses
            • Uterus / pathology

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