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Home / Equine Research Bank / J Vet Med / Comparison of the Effect of Heterologous and Homologous Semi...
Journal of veterinary medicine2014; 2014; 325451; doi: 10.1155/2014/325451

Comparison of the Effect of Heterologous and Homologous Seminal Plasma on Motility and Chromatin Integrity of Stallion Spermatozoa Selected by Single Layer Centrifugation.

Abstract: The effect on sperm motility and chromatin integrity of adding homologous or heterologous equine seminal plasma (SP) to fresh stallion spermatozoa selected by single layer centrifugation (SLC) was studied. No statistical difference in mean progressive motility was seen after adding SP at time 0 h, although there were differences for individual stallions. The proportion of spermatozoa with high velocity was increased compared to untreated SLC-selected spermatozoa (P < 0.05), with significant differences between individuals (P < 0.01). When the SLC samples were stored for 24 h before adding SP, a significant increase in mean progressive motility was seen for SLC + homologous SP (P < 0.01) and for SLC + heterologous SP (P < 0.056). Whether homologous SP or heterologous SP had a greater effect on progressive motility depended on the individual. Adding either type of SP caused a significant increase in chromatin damage compared to SLC after storage for 24 h (homologous SP, P < 0.05; heterologous SP, P < 0.01). These preliminary data showed that storage of SLC-spermatozoa mixed with SP should be avoided because of the risk of increased chromatin damage. If SP is to be added to take advantage of a transient increase in progressive motility for a particular individual stallion, different combinations of SP and spermatozoa should be tested first to optimize the effect.
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Publication Date: 2014-08-20 PubMed ID: 26464926PubMed Central: PMC4590871DOI: 10.1155/2014/325451Google Scholar: Lookup
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Summary

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The study analyzes the influence of adding either homologous or heterologous seminal plasma to stallion sperm following selection by single layer centrifugation (SLC) on the motility and chromatin integrity of the sperm. The research suggests that although there perceived to be a temporary improvement in sperm motility, the addition of seminal plasma significantly increases chromatin damage, thereby posing a risk for sperm storage.

Background of the Study

  • The research is based on the exploration of the effects of seminal plasma (SP), a substance that contains sperm cells released during ejaculation, when added to stallion spermatozoa that’s been selected via a method called single layer centrifugation (SLC).
  • The seminal plasma types studied are homologous (from the same species) and heterologous (from a different species).
  • Particularly, the study focuses on the impact of these two types of SP on the motility (the ability to move spontaneously) and chromatin integrity (the damage to the sperm DNA) of stallion sperm.

Major Findings

  • Initial addition of SP to the stallion sperm didn’t show notable difference in mean progressive motility (a parameter for sperm health) across the board, though some individual variation was observed.
  • Interestingly, the proportion of spermatozoa with high velocity experienced an increase compared to those that were untreated.
  • When stored for 24 hours before adding SP, a noticeable increase in mean progressive motility was observed for both SLC-treated sperm mixed with homologous SP and heterologous SP.
  • Nevertheless, the effect of each type of SP on progressive motility seemed to differ depending on the individual stallion.
  • Significantly, addition of either type of SP resulted in a considerable rise in chromatin damage after storage for 24 hours.

Implications of the Study

  • The preliminary data from this study suggests caution when storing SLC-selected sperm mixed with SP due to the potential for increased chromatin damage, which can affect the quality and viability of the sperm.
  • If SP is to be added to improve motility temporarily for a specific individual stallion, it’s recommended to test different combinations of SP and spermatozoa first to optimize the results.

Overall, this study throws light on the intricate balance between sperm health and the application of seminal plasma in sperm’s storage and motility.

Cite This Article

APA
Morrell JM, Johannisson A. (2014). Comparison of the Effect of Heterologous and Homologous Seminal Plasma on Motility and Chromatin Integrity of Stallion Spermatozoa Selected by Single Layer Centrifugation. J Vet Med, 2014, 325451. https://doi.org/10.1155/2014/325451

Publication

Journal of veterinary medicine
ISSN: 2356-7708
NlmUniqueID: 101627989
Country: United States
Language: English
Volume: 2014
Pages: 325451
PII: 325451

Researcher Affiliations

Morrell, J M
  • Clinical Sciences, Swedish University of Agricultural Sciences (SLU), P.O. Box 7054, 75007 Uppsala, Sweden.
Johannisson, A
  • Department of Anatomy, Physiology and Biochemistry, SLU, 75007 Uppsala, Sweden.

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