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Home / Equine Research Bank / Front Vet Sci / Comparison of the Effects of Interleukin-1 on Equine Articul...
Frontiers in veterinary science2017; 4; 152; doi: 10.3389/fvets.2017.00152

Comparison of the Effects of Interleukin-1 on Equine Articular Cartilage Explants and Cocultures of Osteochondral and Synovial Explants.

Abstract: Osteoarthritis (OA) is a ubiquitous disease affecting many horses. The disease causes chronic pain and decreased performance for patients and great cost to owners for diagnosis and treatment. The most common treatments include systemic non-steroidal anti-inflammatory drugs and intra-articular injection of corticosteroids. There is excellent support for the palliative pain relief these treatments provide; however, they do not arrest progression and may in some instances hasten advancement of disease. Orthobiologic treatments have been investigated as potential OA treatments that may not only ameliorate pain but also prevent or reverse pathologic articular tissue changes. Clinical protocols for intra-articular use of such treatments have not been optimized; the high cost of in vivo research and concerns over humane use of research animals may be preventing discovery. The objective of this study was to evaluate a novel in vitro articular coculture system for future use in OA treatment research. Concentrations and fold increases in various markers of inflammation (prostaglandin E2 and tumor necrosis factor-alpha), degradative enzyme activity [matrix metalloproteinase-13 (MMP-13)], cartilage and bone metabolism (bone alkaline phosphatase and dimethyl-methylene blue), and cell death (lactate dehydrogenase) were compared between IL-1-stimulated equine articular cartilage explant cultures and cocultures comprised of osteochondral and synovial explants (OCS). Results suggested that there are differences in responses of culture systems to inflammatory stimulation. In particular, the IL-1-induced fold changes in MMP-13 concentration were significantly different between OCS and cartilage explant culture systems after 96 h. These differences may be relevant to responses of joints to inflammation in vivo and could be important to the biological relevance of in vitro research findings.
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Publication Date: 2017-09-20 PubMed ID: 28979900PubMed Central: PMC5611359DOI: 10.3389/fvets.2017.00152Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article presents a study comparing the effects of Interleukin-1 on equine articular cartilage and a coculture system comprised of osteochondral and synovial explants. The objective was to evaluate a new method for osteoarthritis treatment research.

Introduction and Background

  • Osteoarthritis (OA) is a common disease in horses, causing chronic pain, decrease in performance, and high costs associated with diagnosis and treatment.
  • Conventional treatments like non-steroidal anti-inflammatory drugs and corticosteroids injections provide temporary relief but do not halt the progression of the disease, and sometimes may even accelerate it.
  • The study therefore investigates orthobiologic treatments that can potentially not only relieve pain but also reverse or prevent pathologic tissue changes.
  • Clinical protocols for such treatments are yet to be optimized due to the high cost of research and concerns over the humane use of research animals.

Methodology

  • The research introduces a novel articular coculture system as a potential tool for OA treatment research.
  • The system was tested using inflamation markers (prostaglandin E, tumor necrosis factor-alpha), a degradative enzyme (matrix metalloproteinase-13 or MMP-13), indicators of cartilage and bone metabolism (bone alkaline phosphatase and dimethyl-methylene blue), and a marker of cell death (lactate dehydrogenase) across two different culture systems.
  • The two systems compared were the IL-1-stimulated equine articular cartilage explant cultures and cocultures comprised of osteochondral and synovial explants.

Findings

  • The outcome suggested differences in the way the two culture systems responded to inflammatory stimulation.
  • Specifically, the changes in MMP-13 concentration, which was induced by IL-1, were considerably different between the two systems after 96 hours.
  • The distinction in these responses may have implications for the way joints respond to inflammation, making these findings potentially vital to the relevance of research in this area.

Cite This Article

APA
Byron CR, Trahan RA. (2017). Comparison of the Effects of Interleukin-1 on Equine Articular Cartilage Explants and Cocultures of Osteochondral and Synovial Explants. Front Vet Sci, 4, 152. https://doi.org/10.3389/fvets.2017.00152

Publication

Frontiers in veterinary science
ISSN: 2297-1769
NlmUniqueID: 101666658
Country: Switzerland
Language: English
Volume: 4
Pages: 152

Researcher Affiliations

Byron, Christopher R
  • Department of Large Animal Clinical Sciences, Virginia-Maryland College of Veterinary Medicine, Virginia Tech, Blacksburg, VA, Unites States.
Trahan, Richard A
  • Department of Large Animal Clinical Sciences, Virginia-Maryland College of Veterinary Medicine, Virginia Tech, Blacksburg, VA, Unites States.

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Citations

This article has been cited 7 times.
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