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Home / Equine Research Bank / Vet Sci / Comparison of the Fecal Microbiota of Horses with Intestinal...
Veterinary sciences2021; 8(6); doi: 10.3390/vetsci8060113

Comparison of the Fecal Microbiota of Horses with Intestinal Disease and Their Healthy Counterparts.

Abstract: (1) Background: The intestinal microbiota plays an essential role in maintaining the host's health. Dysbiosis of the equine hindgut microbiota can alter the fermentation patterns and cause metabolic disorders. (2) Methods: This study compared the fecal microbiota composition of horses with intestinal disease and their healthy counterparts living in Korea using 16S rRNA sequencing from fecal samples. A total of 52 fecal samples were collected and divided into three groups: horses with large intestinal disease (n = 20), horses with small intestinal disease (n = 8), and healthy horses (n = 24). (3) Results: Horses with intestinal diseases had fewer species and a less diverse bacterial population than healthy horses. Lactic acid bacteria, Lachnospiraceae, and Lactobacillaceae were overgrown in horses with large intestinal colic. The Firmicutes to Bacteroidetes ratio (F/B), which is a relevant marker of gut dysbiosis, was 1.94, 2.37, and 1.74 for horses with large intestinal colic, small intestinal colic, and healthy horses, respectively. (4) Conclusions: The overgrowth of two lactic acid bacteria families, Lachnospiraceae and Lactobacillaceae, led to a decrease in hindgut pH that interfered with normal fermentation, which might cause large intestinal colic. The overgrowth of Streptococcus also led to a decrease in pH in the hindgut, which suppressed the proliferation of the methanogen and reduced methanogenesis in horses with small intestinal colic.
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Publication Date: 2021-06-17 PubMed ID: 34204317PubMed Central: PMC8234941DOI: 10.3390/vetsci8060113Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article explains a study that compared the intestinal bacteria of healthy horses and those with intestinal diseases in Korea. The results showed different patterns of bacteria between healthy and sick horses, suggesting a link between certain bacteria and intestinal diseases.

Background of the Study

  • The researchers opened by discussing the crucial role that intestinal bacteria (known as the microbiota) play in maintaining an organism’s health.
  • They noted that imbalances in the equine (horse) gut microbiota, called dysbiosis, could change fermentation patterns and lead to metabolic disorders.

Methods Implemented

  • The study involved comparing the composition of fecal bacteria in horses with intestinal diseases and healthy horses in Korea.
  • This comparison was done through sequencing of a part of the bacterial RNA (16S rRNA) from the horses’ fecal samples.
  • Fifty-two samples were collected in total, with 20 from horses with large intestinal diseases, 8 from horses with small intestinal diseases, and 24 from healthy horses.

Findings of the Study

  • The researchers found that horses with intestinal diseases had more species of bacteria and lower diversity in their bacterial population than healthy horses.
  • High populations of lactic acid bacteria, Lachnospiraceae, and Lactobacillaceae were found in horses suffering from large intestinal colic (a type of abdominal pain).
  • A ratio known as the Firmicutes to Bacteroidetes ratio (F/B), which is a marker of gut imbalance, was found to be highest in horses with small intestinal diseases.

Conclusions from the Study

  • The researchers concluded that an overpopulation of two types of lactic acid bacteria – Lachnospiraceae and Lactobacillaceae – caused a decrease in the pH levels in a horse’s hindgut (the latter part of the intestine).
  • This decreased pH interfered with the normal fermentation process potentially leading to large intestinal colic.
  • An overpopulation of the same bacteria in the hindgut also caused a pH decrease that inhibited the growth of a type of microorganism called methanogens, resulting in less methanogenesis (production of methane), potentially leading to small intestinal colic.

Cite This Article

APA
Park T, Cheong H, Yoon J, Kim A, Yun Y, Unno T. (2021). Comparison of the Fecal Microbiota of Horses with Intestinal Disease and Their Healthy Counterparts. Vet Sci, 8(6). https://doi.org/10.3390/vetsci8060113

Publication

Veterinary sciences
ISSN: 2306-7381
NlmUniqueID: 101680127
Country: Switzerland
Language: English
Volume: 8
Issue: 6

Researcher Affiliations

Park, Taemook
  • Equine Clinic, Jeju Stud Farm, Korea Racing Authority, Jeju 63346, Korea.
  • College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University, Jeju 63243, Korea.
Cheong, Heetae
  • College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 24341, Korea.
Yoon, Jungho
  • Equine Clinic, Jeju Stud Farm, Korea Racing Authority, Jeju 63346, Korea.
Kim, Ahram
  • Equine Clinic, Jeju Stud Farm, Korea Racing Authority, Jeju 63346, Korea.
Yun, Youngmin
  • College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University, Jeju 63243, Korea.
Unno, Tatsuya
  • Faculty of Biotechnology, School of Life Sciences, SARI, Jeju 63243, Korea.
  • Subtropical/Tropical Organism Gene Bank, Jeju National University, Jeju 63243, Korea.

Grant Funding

  • 2016R1A6A1A03012862 / National Research Foundation of Korea

Conflict of Interest Statement

The authors declare no conflict of interest.

References

This article includes 52 references
  1. Costa M.C., Weese J.S.. The equine intestinal microbiome. Anim. Health Res. Rev. 2012;13:121–128.
    doi: 10.1017/S1466252312000035pubmed: 22626511google scholar: lookup
  2. Julliand V., Grimm P.. Horse Species Symposium: The microbiome of the horse hindgut: History and current knowledge. J. Anim. Sci. 2016;94:2262–2274.
    doi: 10.2527/jas.2015-0198pubmed: 27285903google scholar: lookup
  3. Julliand V., De Fombelle A., Drogoul C., Jacotot E.. Feeding and microbial disorders in horses: Part 3—Effects of three hay: Grain ratios on microbial profile and activities. J. Equine Vet. Sci. 2001;21:543–546.
    doi: 10.1016/S0737-0806(01)70159-1google scholar: lookup
  4. Coverdale J.. Horse Species Symposium: Can the microbiome of the horse be altered to improve digestion?. J. Anim. Sci. 2016;94:2275–2281.
    doi: 10.2527/jas.2015-0056pubmed: 27285904google scholar: lookup
  5. Jensen R., Austbø D., Blache D., Knudsen K.B., Tauson A.-H.. The effect of feeding barley or hay alone or in combination with molassed sugar beet pulp on the metabolic responses in plasma and caecum of horses. Anim. Feed Sci. Technol. 2016;214:53–65.
    doi: 10.1016/j.anifeedsci.2016.02.003google scholar: lookup
  6. Chapman A.M.. Acute diarrhea in hospitalized horses. Vet. Clin. N. Am. Equine Pract. 2009;25:363–380.
    doi: 10.1016/j.cveq.2009.05.001pubmed: 19580946google scholar: lookup
  7. Vieira S.M., Hiltensperger M., Kumar V., Zegarra-Ruiz D., Dehner C., Khan N., Costa F.R.C., Tiniakou E., Greiling T., Ruff W.. Translocation of a gut pathobiont drives autoimmunity in mice and humans. Science 2018;359:1156–1161.
    doi: 10.1126/science.aar7201pmc: PMC5959731pubmed: 29590047google scholar: lookup
  8. Mazmanian S.K.. Capsular polysaccharides of symbiotic bacteria modulate immune responses during experimental colitis. J. Pediatric Gastroenterol. Nutr. 2008;46:E11–E12.
    doi: 10.1097/01.mpg.0000313824.70971.a7pubmed: 18354314google scholar: lookup
  9. Paun A., Danska J.S.. Immuno-ecology: How the microbiome regulates tolerance and autoimmunity. Curr. Opin. Immunol. 2015;37:34–39.
    doi: 10.1016/j.coi.2015.09.004pubmed: 26460968google scholar: lookup
  10. Wu H.J., Wu E.. The role of gut microbiota in immune homeostasis and autoimmunity. Gut Microbes 2012;3:4–14.
    doi: 10.4161/gmic.19320pmc: PMC3337124pubmed: 22356853google scholar: lookup
  11. Milinovich G.J., Klieve A.V., Pollitt C.C., Trott D.J.. Microbial events in the hindgut during carbohydrate-induced equine laminitis. Vet. Clin. N. Am. Equine Pract. 2010;26:79–94.
    doi: 10.1016/j.cveq.2010.01.007pubmed: 20381737google scholar: lookup
  12. Fujimoto T., Imaeda H., Takahashi K., Kasumi E., Bamba S., Fujiyama Y., Andoh A.. Decreased abundance of Faecalibacterium prausnitzii in the gut microbiota of Crohn’s disease. J. Gastroenterol. Hepatol. 2013;28:613–619.
    doi: 10.1111/jgh.12073pubmed: 23216550google scholar: lookup
  13. Giamarellos-Bourboulis E., Tang J., Pyleris E., Pistiki A., Barbatzas C., Brown J., Lee C.C., Harkins T.T., Kim G., Weitsman S.. Molecular assessment of differences in the duodenal microbiome in subjects with irritable bowel syndrome. Scand. J. Gastroenterol. 2015;50:1076–1087.
    doi: 10.3109/00365521.2015.1027261pubmed: 25865706google scholar: lookup
  14. Ahn J., Sinha R., Pei Z., Dominianni C., Wu J., Shi J., Goedert J.J., Hayes R.B., Yang L.. Human gut microbiome and risk for colorectal cancer. J. Natl. Cancer Inst. 2013;105:1907–1911.
    doi: 10.1093/jnci/djt300pmc: PMC3866154pubmed: 24316595google scholar: lookup
  15. Zheng P., Li Z., Zhou Z.. Gut microbiome in type 1 diabetes: A comprehensive review. Diabetes Metab. Res. Rev. 2018;34:e3043.
    doi: 10.1002/dmrr.3043pmc: PMC6220847pubmed: 29929213google scholar: lookup
  16. Aydin Ö., Nieuwdorp M., Gerdes V.. The gut microbiome as a target for the treatment of type 2 diabetes. Curr. Diabetes Rep. 2018;18:1–11.
    doi: 10.1007/s11892-018-1020-6pmc: PMC6013535pubmed: 29931613google scholar: lookup
  17. Asano Y., Hiramoto T., Nishino R., Aiba Y., Kimura T., Yoshihara K., Koga Y., Sudo N.. Critical role of gut microbiota in the production of biologically active, free catecholamines in the gut lumen of mice. Am. J. Physiol. Gastrointest. Liver Physiol. 2012;303:G1288–G1295.
    doi: 10.1152/ajpgi.00341.2012pubmed: 23064760google scholar: lookup
  18. Sudo N., Chida Y., Aiba Y., Sonoda J., Oyama N., Yu X.N., Kubo C., Koga Y.. Postnatal microbial colonization programs the hypothalamic–pituitary–adrenal system for stress response in mice. J. Physiol. 2004;558:263–275.
    doi: 10.1113/jphysiol.2004.063388pmc: PMC1664925pubmed: 15133062google scholar: lookup
  19. Stewart H.L., Pitta D., Indugu N., Vecchiarelli B., Hennessy M.L., Engiles J.B., Southwood L.L.. Changes in the faecal bacterial microbiota during hospitalisation of horses with colic and the effect of different causes of colic. Equine Vet. J. 2020:1–13.
    doi: 10.1111/evj.13389pubmed: 33222287google scholar: lookup
  20. Al Jassim R.A., Andrews F.M.. The bacterial community of the horse gastrointestinal tract and its relation to fermentative acidosis, laminitis, colic, and stomach ulcers. Vet. Clin. N. Am. Equine Pract. 2009;25:199–215.
    doi: 10.1016/j.cveq.2009.04.005pubmed: 19580934google scholar: lookup
  21. Weese J.S., Holcombe S.J., Embertson R.M., Kurtz K.A., Roessner H.A., Jalali M., Wismer S.E.. Changes in the faecal microbiota of mares precede the development of post partum colic. Equine Vet. J. 2015;47:641–649.
    doi: 10.1111/evj.12361pubmed: 25257320google scholar: lookup
  22. Costa M.C., Arroyo L.G., Allen-Vercoe E., Stampfli H.R., Kim P.T., Sturgeon A., Weese J.S.. Comparison of the fecal microbiota of healthy horses and horses with colitis by high throughput sequencing of the V3-V5 region of the 16S rRNA gene. PLoS ONE 2012;7:e41484.
    doi: 10.1371/journal.pone.0041484pmc: PMC3409227pubmed: 22859989google scholar: lookup
  23. Salem S.E., Maddox T.W., Antczak P., Ketley J.M., Williams N.J., Archer D.C.. Acute changes in the colonic microbiota are associated with large intestinal forms of surgical colic. BMC Vet. Res. 2019;15:1–13.
    doi: 10.1186/s12917-019-2205-1pmc: PMC6925886pubmed: 31864369google scholar: lookup
  24. Dougal K., de la Fuente G., Harris P.A., Girdwood S.E., Pinloche E., Newbold C.J.. Identification of a core bacterial community within the large intestine of the horse. PLoS ONE 2013;8:e77660.
    doi: 10.1371/journal.pone.0077660pmc: PMC3812009pubmed: 24204908google scholar: lookup
  25. Biddle A.S., Black S.J., Blanchard J.L.. An in vitro model of the horse gut microbiome enables identification of lactate-utilizing bacteria that differentially respond to starch induction. PLoS ONE 2013;8:e77599.
    doi: 10.1371/journal.pone.0077599pmc: PMC3788102pubmed: 24098591google scholar: lookup
  26. Rodriguez C., Taminiau B., Brévers B., Avesani V., Van Broeck J., Leroux A., Gallot M., Bruwier A., Amory H., Delmée M.. Faecal microbiota characterisation of horses using 16 rdna barcoded pyrosequencing, and carriage rate of clostridium difficile at hospital admission. BMC Microbiol. 2015;15:1–14.
    doi: 10.1186/s12866-015-0514-5pmc: PMC4573688pubmed: 26377067google scholar: lookup
  27. Milinovich G.J., Trott D.J., Burrell P.C., Croser E.L., Al Jassim R.A., Morton J.M., Van Eps A.W., Pollitt C.C.. Fluorescence in situ hybridization analysis of hindgut bacteria associated with the development of equine laminitis. Environ. Microbiol. 2007;9:2090–2100.
    doi: 10.1111/j.1462-2920.2007.01327.xpubmed: 17635552google scholar: lookup
  28. Steelman S.M., Chowdhary B.P., Dowd S., Suchodolski J., Janečka J.E.. Pyrosequencing of 16S rRNA genes in fecal samples reveals high diversity of hindgut microflora in horses and potential links to chronic laminitis. BMC Vet. Res. 2012;8:1–11.
    doi: 10.1186/1746-6148-8-231pmc: PMC3538718pubmed: 23186268google scholar: lookup
  29. Daly K., Proudman C.J., Duncan S.H., Flint H.J., Dyer J., Shirazi-Beechey S.P.. Alterations in microbiota and fermentation products in equine large intestine in response to dietary variation and intestinal disease. Br. J. Nutr. 2012;107:989–995.
    doi: 10.1017/S0007114511003825pubmed: 21816118google scholar: lookup
  30. Uzal F.A., Diab S.S.. Gastritis, enteritis, and colitis in horses. Vet. Clin. N. Am. Equine Pract. 2015;31:337–358.
    doi: 10.1016/j.cveq.2015.04.006pmc: PMC7127504pubmed: 26048413google scholar: lookup
  31. Stewart H.L., Pitta D., Indugu N., Vecchiarelli B., Engiles J.B., Southwood L.L.. Characterization of the fecal microbiota of healthy horses. Am. J. Vet. Res. 2018;79:811–819.
    doi: 10.2460/ajvr.79.8.811pubmed: 30058849google scholar: lookup
  32. Caspi R., Billington R., Keseler I.M., Kothari A., Krummenacker M., Midford P.E., Ong W.K., Paley S., Subhraveti P., Karp P.D.. The MetaCyc database of metabolic pathways and enzymes—A 2019 update. Nucleic Acids Res. 2020;48:D445–D453.
    doi: 10.1093/nar/gkz862pmc: PMC6943030pubmed: 31586394google scholar: lookup
  33. O’Donnell M.M., Harris H.M., Ross R.P., O’Toole P.W.. Core fecal microbiota of domesticated herbivorous ruminant, hindgut fermenters, and monogastric animals. Microbiologyopen 2017;6:e00509.
    doi: 10.1002/mbo3.509pmc: PMC5635170pubmed: 28834331google scholar: lookup
  34. Salem S.E., Hough R., Probert C., Maddox T.W., Antczak P., Ketley J.M., Williams N.J., Stoneham S.J., Archer D.C.. A longitudinal study of the faecal microbiome and metabolome of periparturient mares. PeerJ 2019;7:e6687.
    doi: 10.7717/peerj.6687pmc: PMC6451438pubmed: 30976468google scholar: lookup
  35. Zhao Y., Li B., Bai D., Huang J., Shiraigo W., Yang L., Zhao Q., Ren X., Wu J., Bao W.. Comparison of fecal microbiota of Mongolian and Thoroughbred Horses by high-throughput sequencing of the V4 Region of the 16S rRNA gene. Asian Aust. J. Anim. Sci. 2016;29:1345.
    doi: 10.5713/ajas.15.0587pmc: PMC5003997pubmed: 26954132google scholar: lookup
  36. Costa M., Silva G., Ramos R., Staempfli H., Arroyo L., Kim P., Weese J.S.. Characterization and comparison of the bacterial microbiota in different gastrointestinal tract compartments in horses. Vet. J. 2015;205:74–80.
    doi: 10.1016/j.tvjl.2015.03.018pubmed: 25975855google scholar: lookup
  37. Mach N., Foury A., Kittelmann S., Reigner F., Moroldo M., Ballester M., Esquerré D., Rivière J., Sallé G., Gérard P.. The effects of weaning methods on gut microbiota composition and horse physiology. Front. Physiol. 2017;8:535.
    doi: 10.3389/fphys.2017.00535pmc: PMC5524898pubmed: 28790932google scholar: lookup
  38. Liu X., Mao B., Gu J., Wu J., Cui S., Wang G., Zhao J., Zhang H., Chen W.. Blautia—A new functional genus with potential probiotic properties?. Gut Microbes 2021;13:1–21.
    pmc: PMC7872077pubmed: 33525961
  39. Nueno-Palop C., Narbad A.. Probiotic assessment of Enterococcus faecalis CP58 isolated from human gut. Int. J. Food Microbiol. 2011;145:390–394.
    doi: 10.1016/j.ijfoodmicro.2010.12.029pubmed: 21315470google scholar: lookup
  40. Zupancic K., Kriksic V., Kovacevic I., Kovacevic D.. Influence of oral probiotic Streptococcus salivarius K12 on ear and oral cavity health in humans: Systematic review. Probiotics Antimicrob. Proteins 2017;9:102–110.
    doi: 10.1007/s12602-017-9261-2pubmed: 28236205google scholar: lookup
  41. Magne F., Gotteland M., Gauthier L., Zazueta A., Pesoa S., Navarrete P., Balamurugan R.. The Firmicutes/Bacteroidetes Ratio: A Relevant Marker of Gut Dysbiosis in Obese Patients?. Nutrients 2020;12:1474.
    doi: 10.3390/nህ1474pmc: PMC7285218pubmed: 32438689google scholar: lookup
  42. Stewart H.L., Southwood L.L., Indugu N., Vecchiarelli B., Engiles J.B., Pitta D.. Differences in the equine faecal microbiota between horses presenting to a tertiary referral hospital for colic compared with an elective surgical procedure. Equine Vet. J. 2019;51:336–342.
    doi: 10.1111/evj.13010pubmed: 30153353google scholar: lookup
  43. Hussein H., Vogedes L., Fernandez G., Frankeny R.. Effects of cereal grain supplementation on apparent digestibility of nutrients and concentrations of fermentation end-products in the feces and serum of horses consuming alfalfa cubes. J. Anim. Sci. 2004;82:1986–1996.
    doi: 10.2527/2004.8271986xpubmed: 15309945google scholar: lookup
  44. Geor R.J.. Current concepts on the pathophysiology of pasture-associated laminitis. Vet. Clin. N. Am. Equine Pract. 2010;26:265–276.
    doi: 10.1016/j.cveq.2010.06.001pubmed: 20699174google scholar: lookup
  45. Horn M.A., Matthies C., Küsel K., Schramm A., Drake H.L.. Hydrogenotrophic methanogenesis by moderately acid-tolerant methanogens of a methane-emitting acidic peat. Appl. Environ. Microbiol. 2003;69:74–83.
    doi: 10.1128/AEM.69.1.74-83.2003pmc: PMC152423pubmed: 12513979google scholar: lookup
  46. Venable E., Kerley M., Raub R.. Assessment of equine fecal microbial profiles during and after a colic episode using pyrosequencing. J. Equine Vet. Sci. 2013;5:347–348.
    doi: 10.1016/j.jevs.2013.03.066google scholar: lookup
  47. Kim I.S., Hwang M.H., Jang N.J., Hyun S.H., Lee S.T.. Effect of low pH on the activity of hydrogen utilizing methanogen in bio-hydrogen process. Int. J. Hydrog. Energy. 2004;29:1133–1140.
    doi: 10.1016/j.ijhydene.2003.08.017google scholar: lookup
  48. Candela M., Perna F., Carnevali P., Vitali B., Ciati R., Gionchetti P., Rizzello F., Campieri M., Brigidi P.. Interaction of probiotic Lactobacillus and Bifidobacterium strains with human intestinal epithelial cells: Adhesion properties, competition against enteropathogens and modulation of IL-8 production. Int. J. Food Microbiol. 2008;125:286–292.
    doi: 10.1016/j.ijfoodmicro.2008.04.012pubmed: 18524406google scholar: lookup
  49. Dougal K., Harris P.A., Edwards A., Pachebat J.A., Blackmore T.M., Worgan H.J., Newbold C.J.. A comparison of the microbiome and the metabolome of different regions of the equine hindgut. FEMS Microbiol. Ecol. 2012;82:642–652.
    doi: 10.1111/j.1574-6941.2012.01441.xpubmed: 22757649google scholar: lookup
  50. Joblin K., Campbell G.P., Richardson A.J., Stewart C.. Fermentation of barley straw by anaerobic rumen bacteria and fungi in axenic culture and in co-culture with methanogens. Lett. Appl. Microbiol. 1989;9:195–197.
    doi: 10.1111/j.1472-765X.1989.tb00323.xgoogle scholar: lookup
  51. Flint H.J., Scott K.P., Duncan S.H., Louis P., Forano E.. Microbial degradation of complex carbohydrates in the gut. Gut Microbes 2012;3:289–306.
    doi: 10.4161/gmic.19897pmc: PMC3463488pubmed: 22572875google scholar: lookup
  52. Steinberg L.M., Regan J.M.. mcrA-targeted real-time quantitative PCR method to examine methanogen communities. Appl. Environ. Microbiol. 2009;75:4435–4442.
    doi: 10.1128/AEM.02858-08pmc: PMC2704849pubmed: 19447957google scholar: lookup

Citations

This article has been cited 23 times.
  1. Rossi GAM, Sellera FP, Ferraz CM, Carvalho RS, Oliveira APL, Marques CA, Fávaro EBR, Rosa RDS, Silva LAM, Cardozo MV, Stehling EG, Furlan JPR. Antimicrobial-Resistant Enteric Gram-Negative Bacteria Isolated from a Fatal Diarrhea in a Horse: Genomic Characterization of CTX-M-2-Producing Escherichia coli. Antibiotics (Basel) 2025 Nov 21;14(12).
    doi: 10.3390/antibiotics14121185pubmed: 41463689google scholar: lookup
  2. Irving J, Pineau V, Shultz S, Ter Woort F, Julien F, Lambey S, van Erck-Westergren E. Impact of Low-Starch Dietary Modifications on Faecal Microbiota Composition and Gastric Disease Scores in Performance Horses. Animals (Basel) 2025 Jun 28;15(13).
    doi: 10.3390/ani15131908pubmed: 40646806google scholar: lookup
  3. Żak-Bochenek A, Drábková Z, Sergedaite V, Siwińska N, Bajzert J, Pasak D, Chełmońska-Soyta A. Fecal Secretory Immunoglobulin A and Lactate Level as a Biomarker of Mucosal Immune Dysfunction in Horses With Colic. J Vet Intern Med 2025 May-Jun;39(3):e70073.
    doi: 10.1111/jvim.70073pubmed: 40145309google scholar: lookup
  4. Shi Y, Maga EA, Mienaltowski MJ. Fecal microbiota changes associated with pathogenic and non-pathogenic diarrheas in foals. BMC Res Notes 2025 Jan 23;18(1):34.
    doi: 10.1186/s13104-025-07110-9pubmed: 39849534google scholar: lookup
  5. Klinhom S, Kunasol C, Sriwichaiin S, Kerdphoo S, Chattipakorn N, Chattipakorn SC, Thitaram C. Characteristics of gut microbiota profiles in Asian elephants (Elephas maximus) with gastrointestinal disorders. Sci Rep 2025 Jan 8;15(1):1327.
    doi: 10.1038/s41598-025-85495-0pubmed: 39779898google scholar: lookup
  6. Brandi LA, Nunes AT, Faleiros CA, Poleti MD, Oliveira ECM, Schmidt NT, Sousa RLM, Fukumasu H, Balieiro JCC, Brandi RA. Dietary Energy Sources Affect Cecal and Fecal Microbiota of Healthy Horses. Animals (Basel) 2024 Dec 3;14(23).
    doi: 10.3390/ani14233494pubmed: 39682460google scholar: lookup
  7. Bishop RC, Kemper AM, Clark LV, Wilkins PA, McCoy AM. Stability of Gastric Fluid and Fecal Microbial Populations in Healthy Horses under Pasture and Stable Conditions. Animals (Basel) 2024 Oct 16;14(20).
    doi: 10.3390/ani14202979pubmed: 39457909google scholar: lookup
  8. Tuniyazi M, Tang R, Hu X, Zhang N. Methylated tirilazad may mitigate oligofructose-induced laminitis in horses. Front Microbiol 2024;15:1391892.
    doi: 10.3389/fmicb.2024.1391892pubmed: 39386364google scholar: lookup
  9. Sävilammi T, Alakangas RR, Häyrynen T, Uusi-Heikkilä S. Gut Microbiota Profiling as a Promising Tool to Detect Equine Inflammatory Bowel Disease (IBD). Animals (Basel) 2024 Aug 18;14(16).
    doi: 10.3390/ani14162396pubmed: 39199930google scholar: lookup
  10. Moss CD, Wilson AL, Reed KJ, Jennings KJ, Kunz IGZ, Landolt GA, Metcalf J, Engle TE, Coleman SJ. Gene Expression Analysis before and after the Pelvic Flexure in the Epithelium of the Equine Hindgut. Animals (Basel) 2024 Aug 8;14(16).
    doi: 10.3390/ani14162303pubmed: 39199837google scholar: lookup
  11. Liu P, Luo Y, Zhang M. Intestinal microbiota and tuberculosis: Insights from Mendelian randomization. Medicine (Baltimore) 2024 Jul 5;103(27):e38762.
    doi: 10.1097/MD.0000000000038762pubmed: 38968531google scholar: lookup
  12. Park T, Yoon J, Yun Y, Unno T. Comparison of the fecal microbiota with high- and low performance race horses. J Anim Sci Technol 2024 Mar;66(2):425-437.
    doi: 10.5187/jast.2023.e45pubmed: 38628692google scholar: lookup
  13. Boucher L, Leduc L, Leclère M, Costa MC. Current Understanding of Equine Gut Dysbiosis and Microbiota Manipulation Techniques: Comparison with Current Knowledge in Other Species. Animals (Basel) 2024 Feb 28;14(5).
    doi: 10.3390/ani14050758pubmed: 38473143google scholar: lookup
  14. Álvarez Narváez S, Beaudry MS, Norris CG, Bartlett PB, Glenn TC, Sanchez S. Improved Equine Fecal Microbiome Characterization Using Target Enrichment by Hybridization Capture. Animals (Basel) 2024 Jan 29;14(3).
    doi: 10.3390/ani14030445pubmed: 38338088google scholar: lookup
  15. Kauter A, Brombach J, Lübke-Becker A, Kannapin D, Bang C, Franzenburg S, Stoeckle SD, Mellmann A, Scherff N, Köck R, Guenther S, Wieler LH, Gehlen H, Semmler T, Wolf SA, Walther B. Antibiotic prophylaxis and hospitalization of horses subjected to median laparotomy: gut microbiota trajectories and abundance increase of Escherichia. Front Microbiol 2023;14:1228845.
    doi: 10.3389/fmicb.2023.1228845pubmed: 38075913google scholar: lookup
  16. Jin Y, Li W, Ba X, Li Y, Wang Y, Zhang H, Li Z, Zhou J. Gut microbiota changes in horses with Chlamydia. BMC Microbiol 2023 Sep 2;23(1):246.
    doi: 10.1186/s12866-023-02986-8pubmed: 37660043google scholar: lookup
  17. Zaitseva S, Dagurova O, Radnagurueva A, Kozlova A, Izotova A, Krylova A, Noskov S, Begmatov S, Patutina E, Barkhutova DD. Fecal Microbiota and Diet Composition of Buryatian Horses Grazing Warm- and Cold-Season Grass Pastures. Microorganisms 2023 Jul 30;11(8).
    doi: 10.3390/microorganisms11081947pubmed: 37630507google scholar: lookup
  18. Souza M, Eeckhaut V, Goossens E, Ducatelle R, Van Nieuwerburgh F, Poulsen K, Baptista AAS, Bracarense APFRL, Van Immerseel F. Guar gum as galactomannan source induces dysbiosis and reduces performance in broiler chickens and dietary β-mannanase restores the gut homeostasis. Poult Sci 2023 Aug;102(8):102810.
    doi: 10.1016/j.psj.2023.102810pubmed: 37343353google scholar: lookup
  19. Thomson P, Pareja J, Núñez A, Santibáñez R, Castro R. Characterization of microbial communities and predicted metabolic pathways in the uterus of healthy mares. Open Vet J 2022 Nov-Dec;12(6):797-805.
    doi: 10.5455/OVJ.2022.v12.i6.3pubmed: 36650865google scholar: lookup
  20. Chaucheyras-Durand F, Sacy A, Karges K, Apper E. Gastro-Intestinal Microbiota in Equines and Its Role in Health and Disease: The Black Box Opens. Microorganisms 2022 Dec 19;10(12).
    doi: 10.3390/microorganisms10122517pubmed: 36557769google scholar: lookup
  21. Bustamante CC, de Paula VB, Rabelo IP, Fernandes CC, Kishi LT, Canola PA, Lemos EGM, Valadão CAA. Effects of Starch Overload and Cecal Buffering on Fecal Microbiota of Horses. Animals (Basel) 2022 Dec 6;12(23).
    doi: 10.3390/ani12233435pubmed: 36496956google scholar: lookup
  22. Wen X, Luo S, Lv D, Jia C, Zhou X, Zhai Q, Xi L, Yang C. Variations in the fecal microbiota and their functions of Thoroughbred, Mongolian, and Hybrid horses. Front Vet Sci 2022;9:920080.
    doi: 10.3389/fvets.2022.920080pubmed: 35968025google scholar: lookup
  23. Lara F, Castro R, Thomson P. Changes in the gut microbiome and colic in horses: Are they causes or consequences?. Open Vet J 2022 Mar-Apr;12(2):242-249.
    doi: 10.5455/OVJ.2022.v12.i2.12pubmed: 35603065google scholar: lookup
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