Comparison of ultrasound and optical coherence tomography techniques for evaluation of integrity of spontaneously repaired horse cartilage.
Abstract: The aim of this study was to compare sensitivity of ultrasound and optical coherence tomography (OCT) techniques for the evaluation of the integrity of spontaneously repaired horse cartilage. Articular surfaces of horse intercarpal joints, featuring both intact tissue and spontaneously healed chondral or osteochondral defects, were imaged ex vivo with arthroscopic ultrasound and laboratory OCT devices. Quantitative ultrasound (integrated reflection coefficient (IRC), apparent integrated backscattering coefficient (AIB) and ultrasound roughness index (URI)) and optical parameters (optical reflection coefficient (ORC), optical roughness index (ORI) and optical backscattering (OBS)) were determined and compared with histological integrity and mechanical properties of the tissue. Spontaneously healed tissue could be quantitatively discerned from the intact tissue with ultrasound and OCT techniques. Furthermore, several significant correlations (p < 0.05) were detected between ultrasound and OCT parameters. Superior resolution of OCT provided a more accurate measurement of cartilage surface roughness, while the ultrasound backscattering from the inner structures of the cartilage matched better with the histological findings. Since the techniques were found to be complementary to each other, dual modality imaging techniques could provide a useful tool for the arthroscopic evaluation of the integrity of articular cartilage.
Publication Date: 2012-03-24 PubMed ID: 22439802DOI: 10.3109/03091902.2012.663054Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article measures the effectiveness of ultrasound and optical coherence tomography (OCT) techniques in evaluating the health of spontaneously repaired horse cartilage, concluding that both methods can identify healed tissue and provide complementary data.
Research Objective
- The main objective of the research was to evaluate and compare the sensitivity of ultrasound and optical coherence tomography (OCT) techniques, specifically in evaluating the integrity of the naturally healed cartilage in horse joints. A focal point within this objective was to decipher just how well these techniques can differentiate between healed and intact tissue.
Process and Methodology
- The researchers assessed the articular surfaces of horse intercarpal joints ex vivo (outside of the organism), selecting samples that included both intact tissue and spontaneously healed chondral or osteochondral defects. This chosen combination aimed to offer a well-rounded view of the tissues of interest.
- These joint surfaces were imaged using arthroscopic ultrasound and laboratory optical coherence tomography (OCT) devices, which are commonly used instruments for non-invasive imaging of biological tissues.
- Quantitative ultrasound including integrated reflection coefficient (IRC), apparent integrated backscouting coefficient (AIB) and ultrasound roughness index (URI), and optical parameters such as optical reflection coefficient (ORC), optical roughness index (ORI) and optical backscouting (OBS) were determined.
Findings
- Both ultrasound and OCT managed to discern spontaneously healed tissue from the intact one, indicating their viability as evaluation tools for the integrity of natural healing in cartilage.
- Furthermore, several significant correlations (p < 0.05) were found between ultrasound and OCT, suggesting that the techniques are complementary.
- OCT was discovered to be superior in providing accurate measurements of cartilage surface roughness due to its higher resolution, while ultrasound backscouting from the inner structures of cartilage matched better with histological findings, which consist of detailed tissue structure analysis under the microscope.
Conclusions
- The research concludes that an integrated dual mode approach that combines ultrasound and OCT could be the most effective method for evaluating the integrity of articular cartilage. By employing both techniques, complete and highly accurate evaluations of healing in horse cartilage could be achieved.
Cite This Article
APA
Virén T, Huang YP, Saarakkala S, Pulkkinen H, Tiitu V, Linjama A, Kiviranta I, Lammi MJ, Brünott A, Brommer H, Van Weeren R, Brama PA, Zheng YP, Jurvelin JS, Töyräs J.
(2012).
Comparison of ultrasound and optical coherence tomography techniques for evaluation of integrity of spontaneously repaired horse cartilage.
J Med Eng Technol, 36(3), 185-192.
https://doi.org/10.3109/03091902.2012.663054 Publication
Researcher Affiliations
- Department of Applied Physics, University of Eastern Finland, Kuopio, Finland. Tuomas.Viren@uef.fi
MeSH Terms
- Animals
- Bone Diseases / diagnosis
- Bone Diseases / diagnostic imaging
- Bone Diseases / pathology
- Bone Diseases / veterinary
- Cartilage Diseases / diagnosis
- Cartilage Diseases / diagnostic imaging
- Cartilage Diseases / pathology
- Cartilage Diseases / veterinary
- Cartilage, Articular / diagnostic imaging
- Cartilage, Articular / injuries
- Cartilage, Articular / pathology
- Cartilage, Articular / physiology
- Horse Diseases / diagnosis
- Horse Diseases / diagnostic imaging
- Horse Diseases / pathology
- Horse Diseases / physiopathology
- Horses
- Statistics, Nonparametric
- Tomography, Optical Coherence / methods
- Tomography, Optical Coherence / veterinary
- Ultrasonography / methods
- Ultrasonography / veterinary
- Wound Healing / physiology
Citations
This article has been cited 11 times.- Ciamillo SA, Pownder SL, Potter HG, Stefanovski D, Nixon AJ, Ortved KF. Correlation of Arthroscopic Grading and Optical Coherence Tomography as Markers of Early Repair and Predictors of Later Healing Evident on MRI and Histomorphometric Assessment of Cartilage Defects Implanted with Chondrocytes Overexpressing IGF-I.. Cartilage 2023 Jun;14(2):210-219.
- Chakrabarti S, Ai M, Henson FMD, Smith ESJ. Peripheral mechanisms of arthritic pain: A proposal to leverage large animals for in vitro studies.. Neurobiol Pain 2020 Aug-Dec;8:100051.
- Sarin JK, Rieppo L, Brommer H, Afara IO, Saarakkala S, Töyräs J. Combination of optical coherence tomography and near infrared spectroscopy enhances determination of articular cartilage composition and structure.. Sci Rep 2017 Sep 6;7(1):10586.
- Puhakka PH, Te Moller NC, Tanska P, Saarakkala S, Tiitu V, Korhonen RK, Brommer H, Virén T, Jurvelin JS, Töyräs J. Optical coherence tomography enables accurate measurement of equine cartilage thickness for determination of speed of sound.. Acta Orthop 2016 Aug;87(4):418-24.
- Jahr H, Brill N, Nebelung S. Detecting early stage osteoarthritis by optical coherence tomography?. Biomarkers 2015;20(8):590-6.
- Mansour JM, Lee Z, Welter JF. Nondestructive Techniques to Evaluate the Characteristics and Development of Engineered Cartilage.. Ann Biomed Eng 2016 Mar;44(3):733-49.
- Nebelung S, Brill N, Tingart M, Pufe T, Kuhl C, Jahr H, Truhn D. Quantitative OCT and MRI biomarkers for the differentiation of cartilage degeneration.. Skeletal Radiol 2016 Apr;45(4):505-16.
- de Bont F, Brill N, Schmitt R, Tingart M, Rath B, Pufe T, Jahr H, Nebelung S. Evaluation of Single-Impact-Induced Cartilage Degeneration by Optical Coherence Tomography.. Biomed Res Int 2015;2015:486794.
- Brill N, Riedel J, Rath B, Tingart M, Jahr H, Betsch M, Quack V, Pufe T, Schmitt R, Nebelung S. Optical coherence tomography-based parameterization and quantification of articular cartilage surface integrity.. Biomed Opt Express 2015 Jul 1;6(7):2398-411.
- Cernohorsky P, Kok AC, Bruin DM, Brandt MJ, Faber DJ, Tuijthof GJ, Kerkhoffs GM, Strackee SD, van Leeuwen TG. Comparison of optical coherence tomography and histopathology in quantitative assessment of goat talus articular cartilage.. Acta Orthop 2015 Apr;86(2):257-63.
- Niemelä T, Virén T, Liukkonen J, Argüelles D, te Moller NC, Puhakka PH, Jurvelin JS, Tulamo RM, Töyräs J. Application of optical coherence tomography enhances reproducibility of arthroscopic evaluation of equine joints.. Acta Vet Scand 2014 Jan 10;56(1):3.
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