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Home / Equine Research Bank / Stand Genomic Sci / Complete genome sequence of Actinobacillus equuli subspecies...
Standards in genomic sciences2015; 10; 32; doi: 10.1186/s40793-015-0009-x

Complete genome sequence of Actinobacillus equuli subspecies equuli ATCC 19392(T).

Abstract: Actinobacillus equuli subsp. equuli is a member of the family Pasteurellaceae that is a common resident of the oral cavity and alimentary tract of healthy horses. At the same time, it can also cause a fatal septicemia in foals, commonly known as sleepy foal disease or joint ill disease. In addition, A. equuli subsp. equuli has recently been reported to act as a primary pathogen in breeding sows and piglets. To better understand how A. equuli subsp. equuli can cause disease, the genome of the type strain of A. equuli subsp. equuli, ATCC 19392(T), was sequenced using the PacBio RSII sequencing system. Its genome is comprised of 2,431,533 bp and is predicted to encode 2,264 proteins and 82 RNAs.
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Publication Date: 2015-06-07 PubMed ID: 26203343PubMed Central: PMC4511653DOI: 10.1186/s40793-015-0009-xGoogle Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper presents the genome sequencing of Actinobacillus equuli subspecies equuli ATCC 19392(T), a bacterium that is typically found in horses but can cause disease in foals and pigs, to help understand its disease-causing mechanisms.

Genomic Information of Actinobacillus equuli subsp. equuli

The genus Actinobacillus, family Pasteurellaceae, includes species such as the A. equuli subsp. equuli that dwell in the oral cavity and alimentary tract of healthy horses. However, the same bacteria can lead to lethal septicemia in foals, typically recognised as sleepy foal disease or joint illness. With the PacBio RSII sequencing platform, the complete DNA sequence or genome of the type strain of this bacteria, ATCC 19392(T), was obtained.

  • The PacBio RSII sequencing system is a sequencer that employs single molecule, real-time (SMRT) technology to deliver highly accurate long reads. SMRT technology can provide crucial insights into the genomic organization of organisms, enabling targeted research into disease-causing mechanisms.
  • The genome of this bacterium is made up of 2,431,533 base pairs (bp). The base pairs are the building blocks of DNA and the number of base pairs gives an indication of the size of the genome.

Protein and RNA Encoding

The genome of A. equuli subsp. equuli ATCC 19392(T) is anticipated to code for a large number of proteins and RNAs.

  • Specifically, it is predicted to encode 2,264 proteins. Proteins are vital for the bacterium’s existence and can offer valuable insights into its metabolism and its pathogenic capabilities. By understanding the proteins that this bacterium encodes for, researchers can get a sense of its potential functions and disease-causing mechanisms.
  • It is also expected to encode 82 RNAs which could include messenger RNA, transfer RNA and ribosomal RNA all of which play critical roles in protein synthesis and regulation of gene expression. Analysis of the predicted RNA can help understand the regulatory mechanisms of the bacterium.

Implications

This research is not just about sequencing the genome of A. equuli subsp. equuli. Understanding the genome gives invaluable information about the biology of the bacterium, its capabilities and its pathogenicity. In addition, it may provide potential drug targets for combating diseases caused by this bacterium. Besides, advanced knowledge about the bacterium might potentially contribute to the development of countermeasures for sleepy foal disease, joint ill disease and diseases in breeding sows and piglets which are reportedly linked with A. equuli subsp. equuli’s pathogenicity.

Cite This Article

APA
Huang BF, Kropinski AM, Bujold AR, MacInnes JI. (2015). Complete genome sequence of Actinobacillus equuli subspecies equuli ATCC 19392(T). Stand Genomic Sci, 10, 32. https://doi.org/10.1186/s40793-015-0009-x

Publication

Standards in genomic sciences
ISSN: 1944-3277
NlmUniqueID: 101530505
Country: England
Language: English
Volume: 10
Pages: 32

Researcher Affiliations

Huang, Barbara F
  • Department of Pathobiology, University of Guelph, Ontario Veterinary College, Ontario N1G 2 W1, Canada.
Kropinski, Andrew M
  • Department of Pathobiology, University of Guelph, Ontario Veterinary College, Ontario N1G 2 W1, Canada.
Bujold, Adina R
  • Department of Pathobiology, University of Guelph, Ontario Veterinary College, Ontario N1G 2 W1, Canada.
MacInnes, Janet I
  • Department of Pathobiology, University of Guelph, Ontario Veterinary College, Ontario N1G 2 W1, Canada.

References

This article includes 49 references
  1. Edwards PR. Studies on Shigella equirulis.. Kentucky Agric Exp Stn Bull 1931;320:289–330.
  2. Sternberg S, Brändström B. Biochemical fingerprinting and ribotyping of isolates of Actinobacillus equuli from healthy and diseased horses.. Vet Microbiol 1999 Mar 31;66(1):53-65.
    doi: 10.1016/S0378-1135(98)00303-4pubmed: 10223322google scholar: lookup
  3. Lentsch RH, Wagner JE. Isolation of Actinobacillus lignieresii and Actinobacillus equuli from laboratory rodents.. J Clin Microbiol 1980 Sep;12(3):351-4.
    pmc: PMC273589pubmed: 7217333doi: 10.1128/jcm.12.3.351-354.1980google scholar: lookup
  4. Ashhurst-Smith C, Norton R, Thoreau W, Peel MM. Actinobacillus equuli septicemia: an unusual zoonotic infection.. J Clin Microbiol 1998 Sep;36(9):2789-90.
    pmc: PMC105212pubmed: 9705442doi: 10.1128/jcm.36.9.2789-2790.1998google scholar: lookup
  5. Blackall PJ, Christensen JP, Bisgaard M. Diversity among isolates of Actinobacillus equuli and related organisms as revealed by ribotyping.. Aust Vet J 1998 Jun;76(6):423-5.
    doi: 10.1111/j.1751-0813.1998.tb12394.xpubmed: 9673769google scholar: lookup
  6. Aalbaek B, Ostergaard S, Buhl R, Jensen HE, Christensen H, Bisgaard M. Actinobacillus equuli subsp. equuli associated with equine valvular endocarditis.. APMIS 2007 Dec;115(12):1437-42.
    doi: 10.1111/j.1600-0463.2007.00768.xpubmed: 18184417google scholar: lookup
  7. Berthoud H, Frey J, Kuhnert P. Characterization of Aqx and its operon: the hemolytic RTX determinant of Actinobacillus equuli.. Vet Microbiol 2002 Jun 20;87(2):159-74.
    doi: 10.1016/S0378-1135(02)00048-2pubmed: 12034544google scholar: lookup
  8. Kuhnert P, Berthoud H, Straub R, Frey J. Host cell specific activity of RTX toxins from haemolytic Actinobacillus equuli and Actinobacillus suis.. Vet Microbiol 2003 Mar 20;92(1-2):161-7.
    doi: 10.1016/S0378-1135(02)00353-Xpubmed: 12488079google scholar: lookup
  9. Sternberg S. Isolation of Actinobacillus equuli from the oral cavity of healthy horses and comparison of isolates by restriction enzyme digestion and pulsed-field gel electrophoresis.. Vet Microbiol 1998 Jan 16;59(2-3):147-56.
    doi: 10.1016/S0378-1135(97)00188-0pubmed: 9549855google scholar: lookup
  10. Pusterla N, Jones ME, Mohr FC, Higgins JK, Mapes S, Jang SS, Samitz EM, Byrne BA. Fatal pulmonary hemorrhage associated with RTX toxin producing Actinobacillus equuli subspecies haemolyticus infection in an adult horse.. J Vet Diagn Invest 2008 Jan;20(1):118-21.
    doi: 10.1177/104063870802000127pubmed: 18182526google scholar: lookup
  11. Matthews S, Dart AJ, Dowling BA, Hodgson JL, Hodgson DR. Peritonitis associated with Actinobacillus equuli in horses: 51 cases.. Aust Vet J 2001 Aug;79(8):536-9.
    doi: 10.1111/j.1751-0813.2001.tb10741.xpubmed: 11599812google scholar: lookup
  12. Patterson-Kane JC, Donahue JM, Harrison LR. Septicemia and peritonitis due to Actinobacillus equuli infection in an adult horse.. Vet Pathol 2001 Mar;38(2):230-2.
    doi: 10.1354/vp.38-2-230pubmed: 11280382google scholar: lookup
  13. Layman QD, Rezabek GB, Ramachandran A, Love BC, Confer AW. A retrospective study of equine actinobacillosis cases: 1999-2011.. J Vet Diagn Invest 2014 May;26(3):365-375.
    doi: 10.1177/1040638714531766pubmed: 24742921google scholar: lookup
  14. Thompson AB, Postey RC, Snider T, Pasma T. Actinobacillus equuli as a primary pathogen in breeding sows and piglets.. Can Vet J 2010 Nov;51(11):1223-5.
    pmc: PMC2957028pubmed: 21286321
  15. Benavente CE, Fuentealba IC. Actinobacillus suis and Actinobacillus equuli, emergent pathogens of septic embolic nephritis, a new challenge for the swine industry.. Arch Med Vet 2012;44:99–107.
    doi: 10.4067/S0301-732X2012000200002google scholar: lookup
  16. Castagnetti C, Rossi M, Parmeggiani F, Zanoni RG, Pirrone A, Mariella J. Facial cellulitis due to Actinobacillus equuli infection in a neonatal foal.. Vet Rec 2008 Mar 15;162(11):347-9.
    doi: 10.1136/vr.162.11.347pubmed: 18344501google scholar: lookup
  17. Public Health England Culture Collections Database. [ http://www.phe-culturecollections.org.uk/]
  18. Dewhirst FE, Paster BJ, Olsen I, Fraser GJ. Phylogeny of 54 representative strains of species in the family Pasteurellaceae as determined by comparison of 16S rRNA sequences.. J Bacteriol 1992 Mar;174(6):2002-13.
    pmc: PMC205807pubmed: 1548238doi: 10.1128/jb.174.6.2002-2013.1992google scholar: lookup
  19. Olsen I, Moller K. Genus II. Actinobacillus. Bergey’s Manual of Systematic Bacteriology, Second Edition, Volume Two, The Proteobacteria, Part B 2005; pp. 866–883.
  20. Christensen H, Bisgaard M, Olsen JE. Reclassification of equine isolates previously reported as Actinobacillus equuli, variants of A. equuli, Actinobacillus suis or Bisgaard taxon 11 and proposal of A. equuli subsp. equuli subsp. nov. and A. equuli subsp. haemolyticus subsp. nov.. Int J Syst Evol Microbiol 2002 Sep;52(Pt 5):1569-1576.
    doi: 10.1099/ijs.0.01637-0pubmed: 12361259google scholar: lookup
  21. Woese CR, Kandler O, Wheelis ML. Towards a natural system of organisms: proposal for the domains Archaea, Bacteria, and Eucarya.. Proc Natl Acad Sci U S A 1990 Jun;87(12):4576-9.
    doi: 10.1073/pnas.87.12.4576pmc: PMC54159pubmed: 2112744google scholar: lookup
  22. Garrity GM, Bell JA, Lilburn T. Phylum XIV. Proteobacteria phyl. nov.. Bergey’s Manual of Systematic Bacteriology, Second Edition, Volume 2, Part B 2005; p. 1.
  23. . Validation of publication of new names and new combinations previously effectively published outside the IJSEM.. Int J Syst Evol Microbiol 2005 May;55(Pt 3):983-985.
    doi: 10.1099/ijs.0.64108-0pubmed: 15879221google scholar: lookup
  24. Garrity GM, Bell JA, Lilburn T. Class III. Gammaproteobacteria class. nov.. Bergey’s Manual of Systematic Bacteriology, Second Edition, Volume 2, Part B 2005; p. 1.
  25. Garrity GM, Bell JA, Lilburn T. Pasteurellales ord. nov.. Bergey’s Manual of Systematic Bacteriology, Second Edition, Volume 2, Part B 2005; p. 850.
  26. List Editor. Validation List no. 7. Validation of the publication of new names and new combinations previously effectively published outside the IJSB.. Int J Syst Bacteriol 1981; 55:382–383.
    doi: 10.1099/00207713-31-3-382google scholar: lookup
  27. Pohl SPD. Dissertation. Phillips-Universität Marburg 1979.
  28. Skerman VBD, McGowan V, Sneath PHA. Approved Lists of Bacterial Names.. Int J Syst Bacteriol 1980;30:225–420.
    doi: 10.1099/00207713-30-1-225google scholar: lookup
  29. Brumpt E. Précis de Parasitologie. 1.. 1910.
  30. van Straaten H. Bacteriologische bevindingen bij eenigo gevallen van pyo-septicaemie (Lähme) der veuluens.. Verslag van den Werksaamheden der Rijksseruminrichting 1916-1917, Rotterdam; 71-76.
  31. Haupt H. Archiv fur wissenschaftliche und praktische Tierheilkunde. 1934; 67:513-524.
  32. Ward CL, Wood JL, Houghton SB, Mumford JA, Chanter N. Actinobacillus and Pasteurella species isolated from horses with lower airway disease.. Vet Rec 1998 Sep 5;143(10):277-9.
    doi: 10.1136/vr.143.10.277pubmed: 9787421google scholar: lookup
  33. MacInnes JI, Lally ET. The Genus Actinobacillus. The Prokaryotes, A Handbook on the Biology of Bacteria: Proteobacteria: Gamma Subclass. 3 2006; pp. 1094–1118.
  34. Ashburner M, Ball CA, Blake JA, Botstein D, Butler H, Cherry JM, Davis AP, Dolinski K, Dwight SS, Eppig JT, Harris MA, Hill DP, Issel-Tarver L, Kasarskis A, Lewis S, Matese JC, Richardson JE, Ringwald M, Rubin GM, Sherlock G. Gene ontology: tool for the unification of biology. The Gene Ontology Consortium.. Nat Genet 2000 May;25(1):25-9.
    doi: 10.1038/75556pmc: PMC3037419pubmed: 10802651google scholar: lookup
  35. Christensen H, Bisgaard M. Revised definition of Actinobacillus sensu stricto isolated from animals. A review with special emphasis on diagnosis.. Vet Microbiol 2004 Mar 26;99(1):13-30.
    doi: 10.1016/j.vetmic.2003.12.002pubmed: 15019108google scholar: lookup
  36. Bruno WJ, Socci ND, Halpern AL. Weighted neighbor joining: a likelihood-based approach to distance-based phylogeny reconstruction.. Mol Biol Evol 2000 Jan;17(1):189-97.
    doi: 10.1093/oxfordjournals.molbev.a026231pubmed: 10666718google scholar: lookup
  37. Cole JR, Chai B, Farris RJ, Wang Q, Kulam-Syed-Mohideen AS, McGarrell DM, Bandela AM, Cardenas E, Garrity GM, Tiedje JM. The ribosomal database project (RDP-II): introducing myRDP space and quality controlled public data.. Nucleic Acids Res 2007 Jan;35(Database issue):D169-72.
    doi: 10.1093/nar/gkl889pmc: PMC1669760pubmed: 17090583google scholar: lookup
  38. Field D, Garrity G, Gray T, Morrison N, Selengut J, Sterk P, Tatusova T, Thomson N, Allen MJ, Angiuoli SV, Ashburner M, Axelrod N, Baldauf S, Ballard S, Boore J, Cochrane G, Cole J, Dawyndt P, De Vos P, DePamphilis C, Edwards R, Faruque N, Feldman R, Gilbert J, Gilna P, Glöckner FO, Goldstein P, Guralnick R, Haft D, Hancock D, Hermjakob H, Hertz-Fowler C, Hugenholtz P, Joint I, Kagan L, Kane M, Kennedy J, Kowalchuk G, Kottmann R, Kolker E, Kravitz S, Kyrpides N, Leebens-Mack J, Lewis SE, Li K, Lister AL, Lord P, Maltsev N, Markowitz V, Martiny J, Methe B, Mizrachi I, Moxon R, Nelson K, Parkhill J, Proctor L, White O, Sansone SA, Spiers A, Stevens R, Swift P, Taylor C, Tateno Y, Tett A, Turner S, Ussery D, Vaughan B, Ward N, Whetzel T, San Gil I, Wilson G, Wipat A. The minimum information about a genome sequence (MIGS) specification.. Nat Biotechnol 2008 May;26(5):541-7.
    doi: 10.1038/nbt1360pmc: PMC2409278pubmed: 18464787google scholar: lookup
  39. Chin CS, Alexander DH, Marks P, Klammer AA, Drake J, Heiner C, Clum A, Copeland A, Huddleston J, Eichler EE, Turner SW, Korlach J. Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data.. Nat Methods 2013 Jun;10(6):563-9.
    doi: 10.1038/nmeth.2474pubmed: 23644548google scholar: lookup
  40. Chaisson MJ, Tesler G. Mapping single molecule sequencing reads using basic local alignment with successive refinement (BLASR): application and theory.. BMC Bioinformatics 2012 Sep 19;13:238.
    doi: 10.1186/1471-2105-13-238pmc: PMC3572422pubmed: 22988817google scholar: lookup
  41. Besemer J, Lomsadze A, Borodovsky M. GeneMarkS: a self-training method for prediction of gene starts in microbial genomes. Implications for finding sequence motifs in regulatory regions.. Nucleic Acids Res 2001 Jun 15;29(12):2607-18.
    doi: 10.1093/nar/29.12.2607pmc: PMC55746pubmed: 11410670google scholar: lookup
  42. Integrated Microbial Genome Database. [ http://img.jgi.doe.gov/]
  43. Markowitz VM, Chen IM, Palaniappan K, Chu K, Szeto E, Pillay M, Ratner A, Huang J, Woyke T, Huntemann M, Anderson I, Billis K, Varghese N, Mavromatis K, Pati A, Ivanova NN, Kyrpides NC. IMG 4 version of the integrated microbial genomes comparative analysis system.. Nucleic Acids Res 2014 Jan;42(Database issue):D560-7.
    doi: 10.1093/nar/gkt963pmc: PMC3965111pubmed: 24165883google scholar: lookup
  44. Zhou Y, Liang Y, Lynch KH, Dennis JJ, Wishart DS. PHAST: a fast phage search tool.. Nucleic Acids Res 2011 Jul;39(Web Server issue):W347-52.
    doi: 10.1093/nar/gkr485pmc: PMC3125810pubmed: 21672955google scholar: lookup
  45. Average Nucleotide Identity Database. [ http://enve-omics.ce.gatech.edu/ani/]
  46. Goris J, Konstantinidis KT, Klappenbach JA, Coenye T, Vandamme P, Tiedje JM. DNA-DNA hybridization values and their relationship to whole-genome sequence similarities.. Int J Syst Evol Microbiol 2007 Jan;57(Pt 1):81-91.
    doi: 10.1099/ijs.0.64483-0pubmed: 17220447google scholar: lookup
  47. Genome-to-Genome Distance Calculator. [ http://ggdc.dsmz.de/distcalc2.php]
  48. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions.. BMC Bioinformatics 2013 Feb 21;14:60.
    doi: 10.1186/1471-2105-14-1pmc: PMC3665452pubmed: 23432962google scholar: lookup
  49. Grant JR, Stothard P. The CGView Server: a comparative genomics tool for circular genomes.. Nucleic Acids Res 2008 Jul 1;36(Web Server issue):W181-4.
    doi: 10.1093/nar/gkn179pmc: PMC2447734pubmed: 18411202google scholar: lookup

Citations

This article has been cited 3 times.
  1. Frey J. RTX Toxins of Animal Pathogens and Their Role as Antigens in Vaccines and Diagnostics.. Toxins (Basel) 2019 Dec 10;11(12).
    doi: 10.3390/toxins11120719pubmed: 31835534google scholar: lookup
  2. Uchida-Fujii E, Niwa H, Kinoshita Y, Nukada T. Actinobacillus species isolated from Japanese Thoroughbred racehorses in the last two decades.. J Vet Med Sci 2019 Sep 3;81(9):1234-1237.
    doi: 10.1292/jvms.19-0192pubmed: 31292334google scholar: lookup
  3. Odelros E, Kendall A, Hedberg-Alm Y, Pringle J. Idiopathic peritonitis in horses: a retrospective study of 130 cases in Sweden (2002-2017).. Acta Vet Scand 2019 Apr 25;61(1):18.
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