Complete Genome Sequence of Escherichia coli Podophage Penshu1.
Abstract: 4s is a Gram-negative bacterium found in the equine intestinal ecosystem alongside diverse other coliform bacteria and bacteriophages. This announcement describes the complete genome of the T7-like 4s podophage Penshu1. From its 39,263-bp genome, 54 protein-encoding genes and a 179-bp terminal repeat were predicted.
Copyright © 2019 Pechacek et al.
Publication Date: 2019-09-19 PubMed ID: 31537683PubMed Central: PMC6753287DOI: 10.1128/MRA.01055-19Google Scholar: Lookup
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Summary
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The research presents the complete genome of a T7-like Escherichia coli bacteriophage, Penshu1, isolated from the equine intestinal ecosystem.
Research Overview
- The research conducted was aimed at sequencing and explaining the complete genome of a T7-like Escherichia coli podophage, known as Penshu1, discovered in the equine, or horse, intestinal ecosystem. The genome has been completely sequenced, allowing for a greater understanding of its structure and function.
The Penshu1 Bacteriophage
- A bacteriophage, or ‘phage’ for short, is a type of virus that invades and replicates within bacteria. The specific type of bacteriophage studied in this research, Penshu1, invades Escherichia coli, a kind of bacteria commonly found in the intestines of warm-blooded organisms.
- The Penshu1 bacteriophage is said to be ‘T7-like’ which means it is similar to the T7 bacteriophage that also targets Escherichia coli bacteria.
Findings of the Study
- After successful sequencing, it was determined that the 4s podophage Penshu1 has a genome length of 39,263 base pairs (bp). Base pairs are the building blocks of DNA, so this gives an indication of the size and complexity of the phage’s genetic material.
- From its genome, a total of 54 protein-encoding genes were predicted. These are regions of DNA that provide instructions for the creation of proteins, which are vital for the phage’s function and survival.
- In addition to the protein-encoding genes, a 179-bp terminal repeat was also predicted. Terminal repeats are sequences of DNA that occur at both ends of a genome. They are crucial for the replication and integration of the phage DNA into the host bacterial cell.
Cite This Article
APA
Pechacek D, Hwangbo M, Moreland R, Liu M, Ramsey J.
(2019).
Complete Genome Sequence of Escherichia coli Podophage Penshu1.
Microbiol Resour Announc, 8(38), e01055-19.
https://doi.org/10.1128/MRA.01055-19 Publication
Researcher Affiliations
- Center for Phage Technology, Texas A&M University, College Station, Texas, USA.
- Center for Phage Technology, Texas A&M University, College Station, Texas, USA.
- Zachry Department of Civil and Environmental Engineering, Texas A&M University, College Station, Texas, USA.
- Center for Phage Technology, Texas A&M University, College Station, Texas, USA.
- Center for Phage Technology, Texas A&M University, College Station, Texas, USA.
- Center for Phage Technology, Texas A&M University, College Station, Texas, USA jolenerr@tamu.edu.
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