Complete Genome Sequences of Six Lactobacilli Isolated from American Quarter Horses.
Abstract: We report the complete circular genome sequences of six strains and their plasmids, if any, from the fecal material of quarter horses at different ages.
Copyright © 2020 Meinders et al.
Publication Date: 2020-11-19 PubMed ID: 33214299PubMed Central: PMC7679092DOI: 10.1128/MRA.00997-20Google Scholar: Lookup
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- Journal Article
Summary
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The research article is about sequencing the whole genome of six strains of Lactobacilli, sourced from the fecal matter of Quarter Horses of varying ages.
Genome Sequencing of Lactobacilli
- The main aim of the study is to thoroughly extract and analyse the complete genetic make-up – the genome – of six distinct strains of the bacterial genus Lactobacillus. This type of bacterium is part of the normal gut microbiota in both humans and animals, and some species are used in the production of fermented dairy products and probiotics.
- The techniques used to achieve this end include DNA extraction from samples, genome sequencing, and then subsequent computations to assemble and compare the genome sequences. The genomes, once sequenced, can provide researchers with a deeper understanding of the genetic makeup of these bacteria and potentially enable the discovery of novel features or mechanisms in their biology.
Source of bacteria: American Quarter Horses
- Interestingly, the bacterial samples were taken from the fecal matter of Quarter Horses. Notably, the researchers took samples from horses of different ages, suggesting an interest in examining any age-related differences in bacterial genetic features.
- This selection of the particular species (American Quarter Horses), and the specific bacterial genus (Lactobacilli), could have significant implications in biology, medicine, and especially veterinarian microbiology. However, the abstract doesn’t share any results or implications of the sequencing, which would be found in the full article.
Possible Presence of Plasmids
- The researchers also noted any presence of plasmids in the Lactobacilli samples. Plasmids are small, circular pieces of DNA that exist separately from the chromosomal DNA within bacteria. They often contain genes that confer some advantage to the bacterium, like drug resistance.
- The remit of the study included the sequencing of these plasmids, where present. Sequencing plasmids can also be of immense research value, as they often carry antibiotic resistance genes or other traits that may impact the host organism’s health or environment.
Cite This Article
APA
Meinders RI, Mendoza M, Dickey AN, Scholl EH, Hassan HM.
(2020).
Complete Genome Sequences of Six Lactobacilli Isolated from American Quarter Horses.
Microbiol Resour Announc, 9(47), e00997-20.
https://doi.org/10.1128/MRA.00997-20 Publication
Researcher Affiliations
- Prestage Department of Poultry Science, North Carolina State University, Raleigh, North Carolina, USA.
- Prestage Department of Poultry Science, North Carolina State University, Raleigh, North Carolina, USA.
- Bioinformatics Research Center, North Carolina State University, Raleigh, North Carolina, USA.
- Bioinformatics Research Center, North Carolina State University, Raleigh, North Carolina, USA.
- Prestage Department of Poultry Science, North Carolina State University, Raleigh, North Carolina, USA hmhassan@ncsu.edu.
References
This article includes 10 references
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