Confocal microscopy of germinal vesicle-stage equine oocytes.
Abstract: The objectives were to compare cumulus type with nucleus form in equine cumulus oocyte complexes (COCs), to define the percentage of germinal vesicle (GV)-stage oocytes within a population of mares, and to further define GV nucleus shapes of equine oocytes. Cumulus types were as follows: 1) compact (56/208, 26.9%), 2) slightly expanded (37/208, 17.8%), 3) moderately expanded (27/208, 13.0%), 4) greatly expanded (15/208, 7.2%), or 5) denuded (73/208, 35.1%). One hundred thirty of 208 COCs (62.5%) were GV-stage, 21/208 (10.1%) were condensed chromatin-stage, 8/208 (3.8%) were polar body-stage, 40/208 (19.2%) were negative (nonstaining), and 9/208 (4.3%) were fragmented. Cumulus types were associated with nucleus forms because higher proportions (P < 0.05) of GV-stage oocytes occurred in compact (42/56, 75.0%), slightly expanded (30/37, 81.1%), moderately expanded (16/27, 59.3%), or denuded (40/73, 54.8%) COCs than in greatly expanded (2/15, 13.3%) COCs. In contrast, lower proportions (P<0.05) of condensed chromatin-stage oocytes occurred in compact (3/56, 5.4%), slightly expanded (0/37, 0.0%), moderately expanded (3/27, 11.1%) or denuded (9/73, 12.3%) COCs than in greatly expanded (6/15, 40.0%) COCs, and lower proportions (P < 0.05) of polar body-stage oocytes occurred in compact (0/56, 0.0%) or denuded (2/73, 2.7%) COCs than in greatly expanded (3/15, 20.0%) COCs. Germinal vesicle-stage equine oocytes had 4 distinct shapes, with higher proportions (P<0.05) having large-regular (54/130, 41.5%) than scattered (10/130, 7.7%), small-round (29/130, 22.3%), or large-irregular (37/130, 28.5%) shapes. Lower proportions (P<0.05) of large-regular GVs occurred in compact (11/42, 26.2%) COCs than in slightly expanded (15/30, 50.0%), or moderately expanded (12/16, 75.0%) COCs. Therefore oocytes with the large-regular GV shape are probably more advanced in development.
Publication Date: 2001-05-17 PubMed ID: 11354703DOI: 10.1016/s0093-691x(01)00491-5Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research provides an analysis of germinal vesicle-stage equine oocytes with different cumulus types, compares the proportion of different ovarian stages in a population of horses, and makes conclusions about the advancement in developmental stages based on germinal vesicle shapes.
Study Objectives
- Compare the cumulus type and nucleus form in equine cumulus oocyte complexes (COCs).
- Define the percentage of germinal vesicle (GV)-stage oocytes within a population of mares.
- Clarify GV nucleus shapes and how they connect to oocyte development.
Cumulus Types
- The cumulus types were identified as compact, slightly expanded, moderately expanded, greatly expanded and denuded.
- The observed mix was as follows: compact (26.9%), slightly expanded (17.8%), moderately expanded (13.0%), greatly expanded (7.2%), and denuded (35.1%).
Distribution of COCs
- Among the 208 COCs, it was found that 62.5% of them were in GV-stage.
- 10.1% were in condensed chromatin-stage, 3.8% in the polar body-stage, 19.2% were negative, and 4.3% were fragmented.
Association of Cumulus Types and Ovarian Stages
- The study found a correlation between the cumulus types and the ovarian stages.
- There were high proportions of GV-stage oocytes present in compact, slightly expanded, moderately expanded, or denuded COCs than in greatly expanded COCs.
- On the other hand, there were significantly fewer condensed chromatin-stage and polar body-stage oocytes present in compact, slightly expanded, moderately expanded, and denuded COCs compared to greatly expanded COCs.
Germinal Vesicle Shapes
- A higher proportion of equine oocytes in germinal vesicle-stage had large-regular shapes (41.5%) than scattered (7.7%), small and round (22.3%), or large and irregular (28.5%) shapes.
- Smaller proportions of oocytes with a large-regular GV shape were found in compact COCs compared to slightly expanded or moderately expanded COCs.
- The proportion and shape of the germinal vesicle were thus seen as indicators of developmental advancement.
Cite This Article
APA
Gable TL, Woods GL.
(2001).
Confocal microscopy of germinal vesicle-stage equine oocytes.
Theriogenology, 55(7), 1417-1430.
https://doi.org/10.1016/s0093-691x(01)00491-5 Publication
Researcher Affiliations
- Department of Animal and Veterinary Science, University of Idaho, Moscow 83844-2201, USA.
MeSH Terms
- Animals
- Cell Nucleus / ultrastructure
- Chromatin / ultrastructure
- Female
- Horses
- Microscopy, Confocal
- Oocytes / ultrastructure
- Ovarian Follicle / ultrastructure
- Ovary / ultrastructure
Citations
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