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Equine veterinary journal2022; 55(1); 92-101; doi: 10.1111/evj.13552

Conservation of vaccine antigen sequences encoded by sequenced strains of Streptococcus equi subsp. equi.

Abstract: Streptococcus equi subspecies equi (S equi) is the cause of Strangles, one of the most prevalent diseases of horses worldwide. Variation within the immunodominant SeM protein has been documented, but a new eight-component fusion protein vaccine, Strangvac, does not contain live S equi or SeM and conservation of the antigens it contains have not been reported. Objective: To define the diversity of the eight Strangvac antigens across a diverse S equi population. Methods: Genomic description. Methods: Antigen sequences from the genomes of 759 S equi isolates from 19 countries, recovered between 1955 and 2018, were analysed. Predicted amino acid sequences in the antigen fragments of SEQ0256(Eq5), SEQ0402(Eq8), SEQ0721(EAG), SEQ0855(SclF), SEQ0935(CNE), SEQ0999(IdeE), SEQ1817(SclI) and SEQ2101(SclC) in Strangvac and SeM were extracted from the 759 assembled genomes and compared. Results: The predicted amino acid sequences of SclC, SclI and IdeE were identical across all 759 genomes. CNE was truncated in the genome of five (0.7%) isolates. SclF was absent from one genome and another encoded a single amino acid substitution. EAG was truncated in two genomes. Eq5 was truncated in four genomes and 123 genomes encoded a single amino acid substitution. Eq8 was truncated in three genomes, one genome encoded four amino acid substitutions and 398 genomes encoded a single amino acid substitution at the final amino acid of the Eq8 antigen fragment. Therefore, at least 1579 (99.9%) of 1580 amino acids in Strangvac were identical in 743 (97.9%) genomes, and all genomes encoded identical amino acid sequences for at least six of the eight Strangvac antigens. Conclusions: Three hundred and seven (40.4%) isolates in this study were recovered from horses in the UK. Conclusions: The predicted amino acid sequences of antigens in Strangvac were highly conserved across this collection of S equi.
© 2022 The Authors. Equine Veterinary Journal published by John Wiley & Sons Ltd on behalf of EVJ Ltd.
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Publication Date: 2022-01-19 PubMed ID: 35000217PubMed Central: PMC10078666DOI: 10.1111/evj.13552Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article is about the testing of a new vaccine, Strangvac, for Strangles, a prevalent disease in horses. The focus of the study is to examine the diversity of the eight antigens (immune response-provoking substances) used in the vaccine across a wide population of the bacteria causing the disease, Streptococcus equi subspecies equi.

Objective and Methodology

  • The primary aim of this research was to assess the diversity of the eight antigens used in the fusion protein vaccine, Strangvac, across a diverse set of Streptococcus equi subspecies equi (S equi), the bacteria responsible for the Strangles disease in horses.
  • The method adopted for this research involved analyzing the antigen sequences within the genomes of 759 different S equi isolates. These isolates were sourced from 19 countries and spanned a temporal range of data from 1955 to 2018.
  • The eight antigen fragments (named SEQ0256(Eq5), SEQ0402(Eq8), SEQ0721(EAG), SEQ0855(SclF), SEQ0935(CNE), SEQ0999(IdeE), SEQ1817(SclI), and SEQ2101(SclC)) used in the Strangvac and SeM vaccines were extracted from the genomes for comparative analysis.

Results of the Study

  • The study found that three of the antigens, SclC, SclI, and IdeE, had identical predicted amino acid sequences across all 759 genomes.
  • A minor proportion of isolates did exhibit some truncations or substitutions in certain antigens like CNE, SclF, EAG, Eq5, and Eq8. However, these variations were minimal and did not significantly affect the overall conservation of antigens in Strangvac.
  • The researchers concluded that at least 99.9% of the total 1580 amino acids in Strangvac were identical in at least 97.9% of the genomes studied. All genomes encoded identical sequences for at least six of the eight antigens in the Strangvac vaccine.

Conclusions Drawn

  • More than 40% of the isolates studied were recovered from horses in the UK.
  • The researchers concluded that the antigens in Strangvac were highly conserved across a diverse array of S equi isolates. In other words, there is a fundamental similarity in the antigens present in the bacterium no matter where or when it was isolated.
  • This conservation is crucial in vaccine development, as it suggests that the Strangvac vaccine might offer a high degree of protection against the Strangles disease in horses regardless of geographic or temporal variations in the pathogen.

Cite This Article

APA
Frosth S, Morris ERA, Wilson H, Frykberg L, Jacobsson K, Parkhill J, Flock JI, Wood T, Guss B, Aanensen DM, Boyle AG, Riihimäki M, Cohen ND, Waller AS. (2022). Conservation of vaccine antigen sequences encoded by sequenced strains of Streptococcus equi subsp. equi. Equine Vet J, 55(1), 92-101. https://doi.org/10.1111/evj.13552

Publication

Equine veterinary journal
ISSN: 2042-3306
NlmUniqueID: 0173320
Country: United States
Language: English
Volume: 55
Issue: 1
Pages: 92-101

Researcher Affiliations

Frosth, Sara
  • Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Morris, Ellen Ruth A
  • Department of Large Animal Clinical Sciences, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, Texas, USA.
Wilson, Hayley
  • University of Cambridge, Cambridge, UK.
Frykberg, Lars
  • Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Jacobsson, Karin
  • Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Parkhill, Julian
  • University of Cambridge, Cambridge, UK.
Flock, Jan-Ingmar
  • Department of Microbiology, Tumour and Cell Biology, Karolinska Institutet, Stockholm, Sweden.
  • Intervacc AB, Stockholm, Sweden.
Wood, Tim
  • Intervacc AB, Stockholm, Sweden.
Guss, Bengt
  • Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Aanensen, David M
  • Big Data Institute, Li Ka Shing Centre for Health Information and Discovery, Nuffield Department of Medicine, University of Oxford, Oxford, UK.
Boyle, Ashley G
  • Department of Clinical Studies New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
Riihimäki, Miia
  • Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Cohen, Noah D
  • Department of Large Animal Clinical Sciences, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, Texas, USA.
Waller, Andrew S
  • Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Uppsala, Sweden.
  • Intervacc AB, Stockholm, Sweden.

MeSH Terms

  • Horses
  • Animals
  • Streptococcus equi / genetics
  • Horse Diseases / epidemiology
  • Streptococcus
  • Streptococcal Infections / prevention & control
  • Streptococcal Infections / veterinary
  • Streptococcal Infections / epidemiology

Grant Funding

  • Department of Large Animal Clinical Sciences
  • USDA-NIFA AgriLife and the Link Equine Research Endowment
  • Texas A&M University
  • Formas
  • Intervacc AB
  • The Horse Trust
  • SEIB
  • Paul Mellon Foundation
  • Alice Noakes Memorial Charitable Trust
  • Ivo Trust
  • Tattersalls
  • The Elise Pilkington Charitable Trust
  • The European Breeders Fund
  • The Serth and Gates Charity
  • Margaret Giffen Charitable Trust
  • The Payne Gallwey Charitable Trust
  • The Stafford Trust
  • Marjorie Coote Animal Charity Trust
  • Beryl Evetts and Robert Luff Animal Welfare Trust
  • The Anne Duchess of Westminster's Charitable Trust
  • S19-741-780 / Petplan Charitable Trust

Conflict of Interest Statement

A.S. Waller, T. Wood and J‐I. Flock are employed by Intervacc AB. B. Guss is a board member of Intervacc AB. S. Frosth, L. Frykberg and K. Jacobsson are funded by a research grant from Intervacc AB. None of the authors has any other financial or personal relationships that could inappropriately influence or bias the content of the paper.

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Citations

This article has been cited 1 times.
  1. Rotinsulu DA, Ewers C, Kerner K, Amrozi A, Soejoedono RD, Semmler T, Bauerfeind R. Molecular Features and Antimicrobial Susceptibilities of Streptococcus equi ssp. equi Isolates from Strangles Cases in Indonesia. Vet Sci 2023 Jan 10;10(1).
    doi: 10.3390/vetsci10010049pubmed: 36669050google scholar: lookup
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