Construction and validation of parentage testing for thoroughbred horses by 53 single nucleotide polymorphisms.
Abstract: We characterized the SNP 53 JPN System for parentage verification during horse registry. The SNP 53 JPN System was constructed using 53 highly polymorphic single nucleotide polymorphisms (SNPs), which were amplified and genotyped with 2 multiplex assays. The SNP 53 JPN System showed good resolution for 95 unrelated thoroughbreds, and the exclusion probability (PE01) for each SNP ranged from 11.5 to 23.0%, resulting in a total PE01 value of 99.996%. These results indicate that the SNP 53 JPN System is useful for parentage testing of thoroughbreds. Of the 53 SNPs, 8 SNPs could be used to exclude a pseudo parent and sib combination found using the 2006 International Society for Animal Genetics (ISAG) horse comparison test, as efficiently as the parentage testing systems using short tandem repeats (STRs). Thus, we concluded that the SNP 53 JPN System could provide sufficient and reliable information for routine parentage testing of thoroughbred.
Publication Date: 2010-02-03 PubMed ID: 20124759DOI: 10.1292/jvms.09-0486Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research article is based on the development and implementation of a new system, known as SNP 53 JPN System, to establish parentage verification in thoroughbred horses through single nucleotide polymorphisms (SNPs). The conclusion drawn from the study suggests that the SNP 53 JPN System is a reliable method for routine parentage testing of thoroughbreds.
Research Objective
- The main objective of this study was to develop and validate a new system for parentage verification in thoroughbred horses. The researchers aimed to use polymorphic single nucleotide polymorphisms (SNPs) to create an efficient system called the SNP 53 JPN System.
Methodology and Findings
- The researchers tested the SNP 53 JPN System using 53 highly polymorphic SNPs. These were amplified and evaluated with two multiplex assays to ascertain their efficiency in determining parentage.
- To further establish the applicability of the SNP 53 JPN System, it was employed on 95 unrelated thoroughbred horses. The system showcased a good resolution with the exclusion probability (PE01) for each SNP ranging from 11.5 to 23.0%, providing a total PE01 value of 99.996%.
- The researchers found that eight of the 53 SNPs could be used to exclude a pseudo-parent and sib combination detected through the International Society for Animal Genetics (ISAG) horse comparison test conducted in 2006. The efficiency level was comparable to the parentage testing systems that used short tandem repeats (STRs).
- These findings point out that the SNP 53 JPN System was rendered robust, providing sufficient and accurate information for routine parentage testing of thoroughbreds.
Conclusion
- The conclusion of the research hinged on the utility of the SNP 53 JPN System in conducting routine parentage testing for thoroughbreds. The study confirmed that the SNP 53 JPN System was competent in providing reliable and efficient results.
Cite This Article
APA
Hirota K, Kakoi H, Gawahara H, Hasegawa T, Tozaki T.
(2010).
Construction and validation of parentage testing for thoroughbred horses by 53 single nucleotide polymorphisms.
J Vet Med Sci, 72(6), 719-726.
https://doi.org/10.1292/jvms.09-0486 Publication
Researcher Affiliations
- Department of Molecular Genetics, Laboratory of Racing Chemistry, Utsunomiya, Tochigi, Japan.
MeSH Terms
- Animals
- Base Sequence
- Breeding / standards
- Chromosomes / genetics
- DNA / blood
- DNA / genetics
- DNA / isolation & purification
- DNA Primers
- Female
- Gene Frequency / genetics
- Genetic Markers
- Genetic Testing / standards
- Genotype
- Horses / genetics
- Male
- Polymerase Chain Reaction
- Polymorphism, Single Nucleotide
- Reproducibility of Results
Citations
This article has been cited 5 times.- Tozaki T, Ohnuma A, Kikuchi M, Ishige T, Kakoi H, Hirota KI, Takahashi Y, Nagata SI. Short Insertion and Deletion Discoveries via Whole-Genome Sequencing of 101 Thoroughbred Racehorses.. Genes (Basel) 2023 Mar 3;14(3).
- Tozaki T, Ohnuma A, Nakamura K, Hano K, Takasu M, Takahashi Y, Tamura N, Sato F, Shimizu K, Kikuchi M, Ishige T, Kakoi H, Hirota KI, Hamilton NA, Nagata SI. Detection of Indiscriminate Genetic Manipulation in Thoroughbred Racehorses by Targeted Resequencing for Gene-Doping Control.. Genes (Basel) 2022 Sep 4;13(9).
- Luttman AM, Komine M, Thaiwong T, Carpenter T, Ewart SL, Kiupel M, Langohr IM, Venta PJ. Development of a 17-Plex of Penta- and Tetra-Nucleotide Microsatellites for DNA Profiling and Paternity Testing in Horses.. Front Vet Sci 2022;9:861623.
- Tozaki T, Ohnuma A, Kikuchi M, Ishige T, Kakoi H, Hirota KI, Kusano K, Nagata SI. Rare and common variant discovery by whole-genome sequencing of 101 Thoroughbred racehorses.. Sci Rep 2021 Aug 6;11(1):16057.
- Kim YC, Jeong BH. The first report of polymorphisms and genetic characteristics of the prion protein gene (PRNP) in horses.. Prion 2018;12(3-4):245-252.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
