Cryopreservation and fertility of ejaculated and epididymal stallion sperm.
Abstract: The cryopreservation of epididymal sperm is important to preserve genetic material from valuable deceased males. This study evaluated the viability of sperm samples from eight stallions under three conditions: (1) collected using an artificial vagina (EJ-0h), (2) recovered from the epididymal cauda immediately after orchiectomy (EP-0h), and (3) recovered from the epididymal cauda after 24h of storage at 5°C (EP-24h). To obtain EJ-0h sperm, two ejaculates were collected from each stallion. After 1 week, the stallions were submitted to bilateral orchiectomy, and one of the removed epididymides was flushed to obtain EP-0h sperm. The contralateral epididymis was stored at 5°C for 24h before being flushed to obtain EP-24h sperm. The sperm samples were analyzed at three different times: immediately after sperm recovery, after dilution in the freezing extender, and post-thawing. A fertility trial was performed using 39 estrous cycles. After ovulation induction with 1mg of deslorelin acetate (i.m.), mares were inseminated with 800×10(6) sperm. The total number of sperm recovered was 7.8±4.7×10(9) for EJ-0h sperm, 12.9±9.2×10(9) for EP-0h sperm and 12.0±8.0×10(9) for EP-24h sperm. The sperm motility, evaluated by total motility, progressive motility and the percentage of rapid cells, was similar among the samples before and after freezing (P>0.05). However, the plasma membrane integrity was different between EJ-0h and EP-0h pre-freezing and between EJ-0h and EP-24h post-thawing (P0.05). In conclusion, the viability and fertility of cauda epididymal sperm are similar to those of ejaculated sperm.
Copyright © 2011 Elsevier B.V. All rights reserved.
Publication Date: 2011-08-16 PubMed ID: 21890290DOI: 10.1016/j.anireprosci.2011.08.002Google Scholar: Lookup
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- Evaluation Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research focuses on the viability and fertility of epididymal sperm from stallions as compared to the traditional ejaculated sperm, and its preservation under different conditions. The results suggested that the fertility of sperm obtained from the epididymal cauda, even after 24 hours of storage at 5°C, is quite similar to traditionally collected ejaculated sperm.
Objective of the Study
- The study was conducted to check the viability of sperm samples collected under three different conditions: ejaculated sperm collected through artificial vagina (EJ-0h), sperm obtained from the epididymal cauda immediately after orchiectomy (EP-0h), and sperm collected from the epididymal cauda after storage for 24 hours at 5°C (EP-24h).
Method of the Study
- Initially, two ejaculates were obtained from each of the eight stallions.
- After a week, bilateral orchiectomy was performed on the stallions and one of the epididymides was cleansed to obtain EP-0h sperm.
- The other epididymis was stored at 5°C for 24 hours and then flushed to obtain EP-24h sperm.
- The sperm samples were analyzed immediately after sperm recovery, after dilution in the freezing extender, and post-thawing.
- A fertility trial was also conducted using 39 estrous cycles. Mares were inseminated with sperm after the induction of ovulation.
Findings of the Study
- The motion rate was almost similar among samples before and after freezing – indicating high sperm motility for all types of samples.
- The rate of conception was found to be similar between groups inseminated with EP-0h, EP-24h, and EJ-0h sperm – indicating similar fertility rate for all types of samples.
- However, plasma membrane integrity was found to be different between EJ-0h and EP-0h before freezing, and also between EJ-0h and EP-24h post thawing. This could be an indication of different cryopreservation patterns.
Conclusion of the Study
- The research concluded that the viability and fertility of sperm from the epididymal cauda are similar to those of ejaculated sperm, proving that the preservation and use of epididymal sperm can be a feasible option in preserving the genetic material of valuable males.
Cite This Article
APA
Monteiro GA, Papa FO, Zahn FS, Dellaqua JA, Melo CM, Maziero RR, Avanzi BR, Alvarenga MA, Guasti PN.
(2011).
Cryopreservation and fertility of ejaculated and epididymal stallion sperm.
Anim Reprod Sci, 127(3-4), 197-201.
https://doi.org/10.1016/j.anireprosci.2011.08.002 Publication
Researcher Affiliations
- Department of Animal Reproduction and Veterinary Radiology, School of Veterinary Medicine and Animal Science, UNESP, Botucatu, SP 18610-970, Brazil. monteiroga@yahoo.com.br
MeSH Terms
- Animals
- Cryopreservation / methods
- Cryopreservation / veterinary
- Ejaculation
- Fertility / physiology
- Horses / physiology
- Male
- Semen Analysis
- Semen Preservation / adverse effects
- Semen Preservation / methods
- Semen Preservation / veterinary
- Sperm Retrieval
- Spermatozoa / cytology
- Spermatozoa / physiology
- Time Factors
Citations
This article has been cited 3 times.- Praxedes ÉCG, Peixoto GCX, Maria da Silva A, Silva AR. Reproduction in agouti (Dasyprocta spp .): A review of reproductive physiology for developing assisted reproductive techniques.. Anim Reprod 2018 Dec 5;15(4):1181-1192.
- Szczykutowicz J, Kałuża A, Kaźmierowska-Niemczuk M, Ferens-Sieczkowska M. The Potential Role of Seminal Plasma in the Fertilization Outcomes.. Biomed Res Int 2019;2019:5397804.
- Scholkamy TH, El-Badry DA, Mahmoud KG. Developmental competence of Dromedary camel oocytes fertilized in vitro by frozen-thawed ejaculated and epididymal spermatozoa.. Iran J Vet Res 2016 Fall;17(4):253-258.
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