Cryopreserved equine amniotic membrane and its use in cutaneous wounds of horses.
Abstract: This study aimed to evaluate the use of equine amniotic membrane (EAM), frozen indirectly using liquid nitrogen and stored between -10° and -24°C, in the treatment of equine skin lesions. Six healthy female horses, aged 3-10 years, were included in this study. EAM was collected from previously evaluated healthy parturient mares. Wounds were surgically created at the distal ends of the forelimbs. One limb was chosen for treatment, and the contralateral limb was chosen as the control. Pain sensitivity, presence of granulation tissue, secretions, and bleeding after debridement during cleaning were evaluated. Microscopically, the following were evaluated: the integrity of the epithelium, the organization of the connective tissue, the presence of hemorrhage, fibroplasia, epithelial hyperplasia, hyperkeratosis, neovascularization, and the types of cells present. Assessments were performed on days 0, 3, 7, 14, 21, 28, and 63, and the time to complete the lesion closure. Treatment with EAM promoted faster recovery, greater neovascularization, better quality fibroplasia, and less sensitivity to pain than the control group. We concluded that the use of EAM was advantageous compared to the control group. O objetivo deste estudo foi avaliar a utilização da membrana amniótica equina (MAE) congelada indiretamente por meio do nitrogênio líquido e conservada entre -10° e -24°C no tratamento de lesões cutâneas de equinos. Foram empregados seis equinos, fêmeas, hígidas, com idades de 3 a 10 anos. A MAE foi coletada de éguas parturientes hígidas, previamente avaliadas. As feridas foram produzidas cirurgicamente nas extremidades distais dos membros anteriores. Um dos membros foi escolhido como tratado, permanecendo o contralateral como controle. Foram avaliados a sensibilidade dolorosa; presença de tecido de granulação; secreções; e hemorragia após desbridamento durante a limpeza. Microscopicamente foram avaliados: a integridade do epitélio, a organização do tecido conjuntivo, a presença de hemorragia, fibroplasia, hiperplasia epitelial, hiperqueratose, neovascularização e os tipos celulares presentes. As avaliações foram realizadas nos dias 0, 3, 7, 14, 21, 28, e 63, e o tempo até o fechamento completo da lesão. O tratamento com MAE promoveu uma recuperação mais rápida que o grupo controle, maior neovascularização, melhor qualidade fibroplasia e menor sensibilidade à dor. Conclui-se que o emprego da MAE foi vantajoso em relação ao grupo controle.
Publication Date: 2022-12-14 PubMed ID: 36533204PubMed Central: PMC9754605DOI: 10.29374/2527-2179.bjvm003122Google Scholar: Lookup
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- Journal Article
Summary
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This study assesses the efficacy of using cryopreserved equine amniotic membrane (EAM) in treating skin lesions on horses, and identifies its benefits such as faster recovery, greater neovascularization, better fibroplasia, and lesser pain sensitivity compared to the control group.
Research Methodology
- Researchers conducted this study using six healthy female horses aged between 3 to 10 years. Cryopreserved EAM, frozen with liquid nitrogen and stored at a temperature between -10° and -24°C, was sourced from healthy parturient mares.
- Surgeons created wounds at the distal ends of the horses’ forelimbs. For each horse, one limb served as the test limb to be treated with EAM, while the other limb acted as a control.
Data Collection and Evaluation
- Data collection consisted of assessments of conditions such as pain sensitivity, the presence of granulation tissue, secretions and bleeding after debridement during cleaning. These evaluations were on days 0, 3, 7, 14, 21, 28, and 63 and also included the total time for complete lesion closure.
- Microscopic evaluations were also done on the integrity of epithelium, the organization of the connective tissue, hemorrhage, fibroplasia, epithelial hyperplasia, hyperkeratosis, neovascularization, and the types of cells present.
Research Findings
- The results showed that wounds treated with EAM recovered faster than those in the control group. This showed the effectiveness of EAM treatment in hastening wound recovery.
- Additionally, the EAM treatment promoted greater neovascularization (formation of new blood vessels) and better quality fibroplasia (production of fibrous connective tissue in wound healing) in the treated limbs as compared to the control ones.
- Further, horses treated with EAM exhibited significantly less sensitivity to pain when compared to horses in the control group.
Conclusion
- The study concludes that the use of cryopreserved EAM for treating skin lesions in horses is indeed beneficial. Compared with a control group, treated horses experienced faster recovery, enhanced formation of new blood vessels, better quality fibroplasia, and decreased pain sensitivity.
Cite This Article
APA
Rosa MVD, Rosa M, Botteon PTL.
(2022).
Cryopreserved equine amniotic membrane and its use in cutaneous wounds of horses.
Braz J Vet Med, 44, e003122.
https://doi.org/10.29374/2527-2179.bjvm003122 Publication
Researcher Affiliations
- Veterinarian, MSc. Programa de Pós-Graduação em Medicina Veterinária (PPGMV), Departamento de Medicina e Cirurgia Veterinária (DMCV), Instituto de Veterinária (IV), Universidade Federal Rural do Rio de Janeiro (UFRRJ). Seropédica, RJ. Brazil.
- Veterinarian, MSc. Programa de Pós-Graduação em Medicina Veterinária (PPGMV), Departamento de Medicina e Cirurgia Veterinária (DMCV), Instituto de Veterinária (IV), Universidade Federal Rural do Rio de Janeiro (UFRRJ). Seropédica, RJ. Brazil.
- Veterinarian, DSc. DMCV, IV, UFRRJ. Seropédica, RJ, Brazil.
Conflict of Interest Statement
Conflict of interests: The authors declare that there was no conflict of interest.
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