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Home / Equine Research Bank / Vet J / Culture and characterisation of equine peripheral blood mese...
Veterinary journal (London, England : 1997)2012; 195(1); 107-113; doi: 10.1016/j.tvjl.2012.05.006

Culture and characterisation of equine peripheral blood mesenchymal stromal cells.

Abstract: Although the use of mesenchymal stromal cells (MSCs) for the treatment of orthopaedic injuries in horses has been reported, no official guidelines exist that classify a particular cell as an equine MSC. Given the limited characterisation of peripheral blood (PB)-derived equine MSCs in particular, this study aimed to provide more detailed information in relation to this cell type. Mesenchymal stromal cells were isolated from equine PB samples and colony forming unit (CFU) assays as well as population doubling times (PDTs) (from P(0) to P(10)) were performed. Two types of colonies, 'fingerprint' and dispersed, could be observed based on macroscopic and microscopic features. Moreover, after an initial lag phase (as indicated by a negative PDT at P(0) to P(1)) the MSCs divided rapidly as indicated by a positive PDT at all further passages. Immunophenotyping was carried out with trypsin- as well as with accutase-detached MSC to evaluate potential trypsin-sensitive epitope destruction on particular antigens. Isolated MSC were positive for CD29, CD44, CD90 and CD105, and negative for CD45, CD79α, MHC II and a monocyte/macrophage marker, irrespective of the cell detaching agent used. Trilineage differentiation of the MSCs towards osteoblasts, chondroblasts and adipocytes was confirmed using a range of histochemical stains.
Copyright © 2012 Elsevier Ltd. All rights reserved.
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Publication Date: 2012-06-18 PubMed ID: 22717781DOI: 10.1016/j.tvjl.2012.05.006Google Scholar: Lookup
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Summary

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The research article discusses a study conducted on equine peripheral blood mesenchymal stromal cells (MSCs) to further understand their characteristics and potential therapeutic uses, particularly for the treatment of orthopaedic injuries in horses.

Objective and Approach

  • The aim of the study was to gather more detailed information on peripheral blood (PB)-derived equine MSCs given the current limited understanding about it.
  • The MSCs were isolated from equine PB samples, and their characteristics observed through colony forming unit (CFU) assays, population doubling times (PDTs) studies, and immunophenotyping.

Observations and Findings

  • In the study, two kinds of cell colonies, ‘fingerprint’ and dispersed, were observed in the isolated MSCs based on macroscopic and microscopic features.
  • The study found a lag phase in the initial period of the equine MSCs’ development process. However, the MSCs showed rapid division as indicated by a positive PDT at all further passages.

Immunophenotyping

  • Immunophenotyping was performed to evaluate potential trypsin-sensitive epitope destruction on specific antigens. This showed that irrespective of the cell detaching agent used, the isolated MSCs were positive for CD29, CD44, CD90, and CD105 and negative for CD45, CD79α, MHC II, and a monocyte/macrophage marker.

Trilineage Differentiation

  • The study also confirmed trilineage differentiation of the MSCs towards osteoblasts, chondroblasts, and adipocytes using a range of histochemical stains.

Conclusion and Potential Applications

  • The research clarified the characteristics of equine peripheral blood mesenchymal stromal cells. This knowledge can be useful for defining guidelines for cell therapy and could potentially improve the treatment for orthopaedic injuries in horses.

Cite This Article

APA
Spaas JH, De Schauwer C, Cornillie P, Meyer E, Van Soom A, Van de Walle GR. (2012). Culture and characterisation of equine peripheral blood mesenchymal stromal cells. Vet J, 195(1), 107-113. https://doi.org/10.1016/j.tvjl.2012.05.006

Publication

Veterinary journal (London, England : 1997)
ISSN: 1532-2971
NlmUniqueID: 9706281
Country: England
Language: English
Volume: 195
Issue: 1
Pages: 107-113
PII: S1090-0233(12)00195-5

Researcher Affiliations

Spaas, Jan H
  • Department of Comparative Physiology and Biometrics, Faculty of Veterinary Medicine, Ghent University, B-9820 Merelbeke, Belgium.
De Schauwer, Catharina
    Cornillie, Pieter
      Meyer, Evelyne
        Van Soom, Ann
          Van de Walle, Gerlinde R

            MeSH Terms

            • Adipocytes
            • Animals
            • Biomarkers / metabolism
            • Cell Adhesion
            • Cell Culture Techniques
            • Chondrocytes
            • Horses / blood
            • Mesenchymal Stem Cells / cytology
            • Mesenchymal Stem Cells / physiology
            • Osteoblasts
            • Plastics
            • Surface Properties

            Citations

            This article has been cited 38 times.
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