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Home / Equine Research Bank / Reprod Biol Endocrinol / Cumulus expansion, nuclear maturation and connexin 43, cyclo...
Reproductive biology and endocrinology : RB&E2004; 2; 44; doi: 10.1186/1477-7827-2-44

Cumulus expansion, nuclear maturation and connexin 43, cyclooxygenase-2 and FSH receptor mRNA expression in equine cumulus-oocyte complexes cultured in vitro in the presence of FSH and precursors for hyaluronic acid synthesis.

Abstract: The aim of this study was to investigate cumulus expansion, nuclear maturation and expression of connexin 43, cyclooxygenase-2 and FSH receptor transcripts in equine cumuli oophori during in vivo and in vitro maturation in the presence of equine FSH (eFSH) and precursors for hyaluronic acid synthesis. Equine cumulus-oocyte complexes (COC) were cultured in a control defined medium supplemented with eFSH (0 to 5 micrograms/ml), Fetal Calf Serum (FCS), precursors for hyaluronic acid synthesis or glutamine according to the experiments. After in vitro maturation, the cumulus expansion rate was increased with 1 microgram/ml eFSH, and was the highest with 20% FCS. It was not influenced by precursors for hyaluronic acid synthesis or glutamine. The expression of transcripts related to cumulus expansion was analyzed in equine cumulus cells before maturation, and after in vivo and in vitro maturation, by using reverse transcription-polymerase chain reaction (RT-PCR) with specific primers. Connexin 43, cyclooxygenase-2 (COX-2) and FSH receptor (FSHr) mRNA were detected in equine cumulus cells before and after maturation. Their level did not vary during in vivo or in vitro maturation and was influenced neither by FSH nor by precursors for hyaluronic acid synthesis. Results indicate that previously reported regulation of connexin 43 and COX-2 proteins during equine COC maturation may involve post-transcriptional mechanisms.
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Publication Date: 2004-06-22 PubMed ID: 15212696PubMed Central: PMC446214DOI: 10.1186/1477-7827-2-44Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article investigates the influence of FSH (Follicle Stimulating Hormone) and precursors for hyaluronic acid synthesis on the growth and signaling pathways within horse cumulus-oocyte complexes (COC). Despite notable effects on the expansion rate, no impacts were found on levels of mRNA related to these pathways.

Overview of Approach

  • In this study, the researchers focused on cumulus expansion, nuclear maturation, and the expression of specific genes (connexin 43, cyclooxygenase-2, and FSH receptor) in equine cumuli oophori. This refers to the cells surrounding an egg in the ovary of a horse.
  • The study differentiated between results observed during in vivo maturation (inside a living organism) and in vitro maturation (outside the body in a controlled environment).
  • The cumulus-oocyte complexes were exposed to different conditions, including the presence of equine FSH, Fetal Calf Serum (FCS), and precursors for hyaluronic acid synthesis.

Main Findings

  • When cultured in vitro, the study found that an increase in cumulus expansion was observed with the administration of 1 microgram/ml of eFSH. The rate of this expansion was at its highest with the addition of 20% Fetal Calf Serum.
  • The rate of cumulus expansion was found to be unaffected by the presence of precursors for the synthesis of hyaluronic acid or glutamine.
  • The researchers used reverse transcription-polymerase chain reaction (RT-PCR) to identify changes in the expression of the genes connexin 43, cyclooxygenase-2 (COX-2), and FSH receptor (FSHr). These genes were found in the equine cumulus cells both before and after their maturation. Furthermore, the presence or absence of FSH or hyaluronic acid precursors did not influence the detected levels of these genes.
  • From these findings, the researchers concluded that the regulation of connexin 43 and COX-2 proteins during equine COC maturation possibly involves post-transcriptional mechanisms, given that no variation was observed at the transcription level.

Cite This Article

APA
Dell'Aquila ME, Caillaud M, Maritato F, Martoriati A, Gérard N, Aiudi G, Minoia P, Goudet G. (2004). Cumulus expansion, nuclear maturation and connexin 43, cyclooxygenase-2 and FSH receptor mRNA expression in equine cumulus-oocyte complexes cultured in vitro in the presence of FSH and precursors for hyaluronic acid synthesis. Reprod Biol Endocrinol, 2, 44. https://doi.org/10.1186/1477-7827-2-44

Publication

Reproductive biology and endocrinology : RB&E
ISSN: 1477-7827
NlmUniqueID: 101153627
Country: England
Language: English
Volume: 2
Pages: 44

Researcher Affiliations

Dell'Aquila, Maria Elena
  • Unité de Physiologie de la Reproduction et des Comportements, UMR 6073 INRA-CNRS-Université F, Rabelais de Tours, 37380 Nouzilly, France. e.dellaquila@veterinaria.uniba.it
Caillaud, Maud
    Maritato, Filippo
      Martoriati, Alain
        Gérard, Nadine
          Aiudi, Giulio
            Minoia, Paolo
              Goudet, Ghylène

                MeSH Terms

                • Animals
                • Cell Nucleus / metabolism
                • Connexin 43 / biosynthesis
                • Cyclooxygenase 2
                • Female
                • Fertilization in Vitro / methods
                • Fertilization in Vitro / veterinary
                • Granulosa Cells / chemistry
                • Granulosa Cells / metabolism
                • Horses / genetics
                • Hyaluronic Acid / biosynthesis
                • Hyaluronic Acid / metabolism
                • Isoenzymes / biosynthesis
                • Oocytes / cytology
                • Oocytes / metabolism
                • Prostaglandin-Endoperoxide Synthases / biosynthesis
                • RNA, Messenger / biosynthesis
                • Receptors, FSH / biosynthesis

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                Citations

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