Curcumin and resveratrol act by different ways on NADPH oxidase activity and reactive oxygen species produced by equine neutrophils.
Abstract: In neutrophils (PMNs), superoxide anion (O2*-), the first reactive oxygen species (ROS) produced to kill pathogenic agents, is generated by NADPH oxidase, an enzymatic complex formed by the translocation of cytosolic subunits to the membrane flavocytochrome b558. In horses, excessive activation of PMNs is often associated with deadly pathologies and the modulation of their ROS production by acting on NADPH oxidase is a prime target to manage inflammation. We developed a cell-free assay to measure the activity of equine NADPH oxidase assembled in vitro, in order to test the effects of natural or synthetic compounds on the enzyme activity or assembly. The cell-free assay was validated with diphenyleneiodonium chloride and Gp91ds-tat, two inhibitors largely described for human NADPH oxidase. The anti-oxidant effects of curcumin and resveratrol at final concentration ranging from 10(-4) to 10(-6) M were studied on whole cells by chemiluminescence (CL) and by cell-free assay, in which the molecule was added before or after the enzyme assembly. The CL assay demonstrated that curcumin efficiently inhibited the O2(-) production and easily entered into PMNs or interacted with their membrane. Cell-free assay showed that curcumin acted on the reconstitution of NADPH oxidase even at 10(-5)M, while resveratrol appeared to be an O2*- scavenger rather than an inhibitor of NADPH oxidase activity, since it acted from outside the cell in CL and after the complex assembly in cell-free assay. By acting directly on NADPH oxidase, curcumin should be a good candidate for the treatment of acute or inflammatory diseases involving an excessive ROS production.
Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.
Publication Date: 2013-09-20 PubMed ID: 24060679DOI: 10.1016/j.cbi.2013.09.011Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This study set out to examine how two antioxidant substances – curcumin and resveratrol – interact with specific neutrophils in horses that produce potentially harmful reactive oxygen species (ROS). The research aimed to provide insights on how these substances may be used to manage inflammation and excessive ROS production in equine health.
Context and Background
- Neutrophils are a type of white blood cell important in defence against bacterial infections.
- In these neutrophils, an enzyme complex called NADPH oxidase generates a reactive oxygen species (ROS) known as superoxide anion. It is one of the first lines of defence against pathogenic agents.
- In horses, excessive activation of these neutrophils is often related to severe diseases.
- Curcumin and resveratrol are both natural compounds known for their antioxidant properties.
Research Methodology
- Researchers developed a “cell-free” assay – a laboratory method for testing a biological substance without involving live cells – to measure equine neutrophil NADPH oxidase activity.
- The assay was tested against two known NADPH oxidase inhibitors.
- Both curcumin and resveratrol were subsequently tested at concentrations between 10(-4) and 10(-6) M, both on the entire cells and in the cell-free assay.
- Chemiluminescence (a method used to measure a chemical reaction that results in the emission of light) was also used to track ROS production in response to these substances.
Findings
- Curcumin was found to efficiently inhibit the production of reactive oxygen species and interacted effectively with the neutrophils, either entering the cells or interacting with their membrane.
- The cell-free assay revealed that curcumin treatment even at 10(-5)M affected the reconstitution of NADPH oxidase.
- On the other hand, resveratrol acted from outside the cells and did not inhibit NADPH oxidase activity, suggesting it is more of a scavenger of the reactive oxygen species rather than an inhibitor of their production.
Implications
- Given its direct interaction with NADPH oxidase, curcumin may be a potent treatment for acute or inflammatory diseases in horses that involve excessive ROS production.
- Despite its different mode of action, resveratrol may also have therapeutic potential in managing excessive ROS production due to its scavenger properties.
Cite This Article
APA
Derochette S, Franck T, Mouithys-Mickalad A, Ceusters J, Deby-Dupont G, Lejeune JP, Neven P, Serteyn D.
(2013).
Curcumin and resveratrol act by different ways on NADPH oxidase activity and reactive oxygen species produced by equine neutrophils.
Chem Biol Interact, 206(2), 186-193.
https://doi.org/10.1016/j.cbi.2013.09.011 Publication
Researcher Affiliations
- Center for Oxygen, R&D (CORD), Institute of Chemistry, B6a, University of Liège, Allée de la Chimie 3, B-4000 Liège, Belgium. Electronic address: sderochette@ulg.ac.be.
MeSH Terms
- Animals
- Cell-Free System
- Curcumin / chemistry
- Curcumin / pharmacology
- Enzyme Inhibitors / chemistry
- Enzyme Inhibitors / pharmacology
- Horses
- Humans
- Luminescent Measurements
- NADPH Oxidases / metabolism
- Neutrophils / drug effects
- Neutrophils / enzymology
- Neutrophils / metabolism
- Reactive Oxygen Species / metabolism
- Resveratrol
- Stilbenes / chemistry
- Stilbenes / pharmacology
Citations
This article has been cited 14 times.- Chen K, Gao Z, Ding Q, Tang C, Zhang H, Zhai T, Xie W, Jin Z, Zhao L, Liu W. Effect of natural polyphenols in Chinese herbal medicine on obesity and diabetes: Interactions among gut microbiota, metabolism, and immunity.. Front Nutr 2022;9:962720.
- Culhuac EB, Elghandour MMMY, Adegbeye MJ, Barbabosa-Pliego A, Salem AZM. Influence of Dietary Selenium on the Oxidative Stress in Horses.. Biol Trace Elem Res 2023 Apr;201(4):1695-1703.
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