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Home / Equine Research Bank / Chromosome Res / Cytogenetics of donkey chromosomes: nomenclature proposal ba...
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology2001; 8(8); 659-670; doi: 10.1023/a:1026707002538

Cytogenetics of donkey chromosomes: nomenclature proposal based on GTG-banded chromosomes and depiction of NORs and telomeric sites.

Abstract: With the expansion of comparative genome analysis across different mammals, there is an increasing need to have well-defined banded karyotypes for the species chosen for investigation. In this context, the steadily growing gene mapping data in the donkey urgently require a framework whereby alignment/comparison of genetic information can be readily made with equids and other mammalian species. Hence a GTG-banded karyotype of the donkey (Equus asinus; EAS) is presented, along with schematic drawings and nomenclature of the banded chromosomes. In addition, the most characteristic features of individual chromosomes are described and their relative size estimated. Using the FISH approach, the location of nucleolous organizer regions (NORs) and telomeric repeat sequences (TTAGGG) were detected. Where possible, information on asine chromosomes is supplemented with known/likely equine and human homologues. The study thus primarily aims to provide an appropriate cytogenetic basis for the donkey chromosomes, so that research focused on gene mapping and comparative genomics in this species can be reported under a common format.
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Publication Date: 2001-02-24 PubMed ID: 11196129DOI: 10.1023/a:1026707002538Google Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article focuses on the creation of a nomenclature proposal, or system of terms, for donkey chromosomes, using the framework of GTG-band karyotypes. The purpose of this study was to aid with the comparison of genetic information with different mammalian species, for example equids and human homologues. The research describes individual chromosome features, estimates their relative size, and identifies the location of nucleolous organizer regions and telomeric repeat sequences via the FISH approach.

Donkey Chromosome Research

  • The research revolves around the necessity for a well-structured banded karyotype or the organization of chromosomes, for species chosen for comparative genome analysis.
  • An increase in gene mapping data in donkeys sparked a requirement for a system where this genetic information could be compared with that of equids (horses and related animals) and other mammalian species.
  • The researchers presented a GTG-banded karyotype of the donkey (Equus asinus or EAS) along with schematic drawings and a proposed nomenclature of these banded chromosomes.

Features of Individual Chromosomes

  • The authors, apart from creating a nomenclature, also described the most characteristic features of the individual chromosomes in the donkey.
  • They estimated the relative size of these chromosomes – an important factor to understand the overall composition and structure of the donkey genome.

Use of FISH Approach

  • To identify specific regions in the chromosomes, researchers used the Fluorescence in situ hybridization (FISH) approach.
  • This approach allowed them to locate the nucleolous organizer regions (NORs) and telomeric repeat sequences (TTAGGG).

Towards Comparative Genomics

  • The research also aimed at providing information on asine chromosomes (donkey chromosomes) supplemented with known or likely equine (horse and related animals) and human homologues.
  • The researchers intended to provide an appropriate cytogenetic basis for the donkey chromosomes which would be beneficial in studies focusing on gene mapping and comparative genomics.

In conclusion, the primary objective behind this research was to create a common format under which research on gene mapping and comparative genomics in the donkey species can be reported.

Cite This Article

APA
Raudsepp T, Christensen K, Chowdhar BP. (2001). Cytogenetics of donkey chromosomes: nomenclature proposal based on GTG-banded chromosomes and depiction of NORs and telomeric sites. Chromosome Res, 8(8), 659-670. https://doi.org/10.1023/a:1026707002538

Publication

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology
ISSN: 0967-3849
NlmUniqueID: 9313452
Country: Netherlands
Language: English
Volume: 8
Issue: 8
Pages: 659-670

Researcher Affiliations

Raudsepp, T
  • Division of Animal Genetics, The Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
Christensen, K
    Chowdhar, B P

      MeSH Terms

      • Animals
      • Chromosome Banding / standards
      • Chromosomes / genetics
      • Cytogenetic Analysis / standards
      • Equidae / genetics
      • Histology, Comparative / standards
      • Horses / genetics
      • Humans
      • In Situ Hybridization, Fluorescence
      • Karyotyping
      • Nucleolus Organizer Region / genetics
      • Nucleolus Organizer Region / ultrastructure
      • Physical Chromosome Mapping / standards
      • Telomere / genetics
      • Telomere / ultrastructure
      • Terminology as Topic

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      Citations

      This article has been cited 12 times.
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