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Cytokines in equine platelet lysate and related blood products.
Abstract: In equine medicine, the use of regenerative therapeutics has gained growing attention, but is still a new and complex field with room for improvement. Platelet lysate (PL) can be used as therapeutic agent but is also a promising supplement for the culture of multipotent mesenchymal stromal cells. To enable a targeted use of PL both in clinic and laboratory, it is crucial to learn more details on its effective ingredients. While so far, mainly growth factor components have been analyzed in platelet-based products such as PL, the current study focuses on the content of cytokines in serum, plasma, platelet concentrate and PL. Blood was harvested from 20 clinically healthy horses and subjected to blood count and chemistry analysis, as well as to further processing to PL. Plasma and platelet concentrate were produced by a buffy-coat-based method and PL was produced from the concentrate by freeze-thawing. Samples from each horse were analyzed regarding interleukin (IL)-1β, -4, -6 and -10, interferon-γ and tumor necrosis factor-α concentrations using sandwich ELISAs. Cytokine concentrations in serum, plasma, concentrate and PL were similar and correlated significantly. However, there was a large inter-individual variability in cytokine concentrations between the different donor horses. The samples from some donor animals had overall very high cytokine concentrations, while samples from other donors had no measurable cytokine ingredient. This pattern was observed for all cytokines. There was a noticeable link between high cytokine concentrations in the blood products and abnormal findings in blood chemistry. Cytokine concentrations in samples from horses with abnormal findings were significantly higher than in samples from the remaining horses. The interindividual differences in cytokine concentrations could be highly relevant when using PL for therapy and cell culture, as the mode of action of the PL is likely changed depending on the presence of pro- and anti-inflammatory cytokines. Blood chemistry might be useful to predict cytokine concentrations in blood products.
Copyright © 2023 Moellerberndt, Hagen, Niebert, Büttner and Burk.
Publication Date: 2023-03-09 PubMed ID: 36968472PubMed Central: PMC10033973DOI: 10.3389/fvets.2023.1117829Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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The research article studies the content of specific proteins called cytokines in horse blood products such as platelet lysate, serum, plasma, and platelet concentrate. The article explores the relationship between cytokine concentrations in these products, their relationship with abnormal blood chemistry findings, and the potential implications for therapeutic use and cell culture.
Objective and Methodology of the Research
- The research aims to investigate cytokine contents in various equine blood products. Cytokines are proteins involved in cell signaling and can trigger both inflammatory and anti-inflammatory responses. The products focused on are platelet lysate (PL), serum, plasma, and platelet concentrate.
- The study was carried out using blood from 20 healthy horses. The blood underwent various tests including blood count, chemistry analysis, and further processing to PL. The plasma and platelet concentrate were generated by using a method known as the buffy-coat-based method, and the PL was produced from the concentrate through freeze-thawing.
- The samples from each horse were analyzed for concentrations of different cytokines, including interleukin (IL)-1β, -4, -6, and -10, interferon-γ, and tumor necrosis factor-α, using method called sandwich ELISAs.
Findings from the Research
- The study found that the cytokine concentrations in serum, plasma, concentrate, and PL were similar and correlated significantly. However, there was substantial variability in cytokine concentrations between different donor horses.
- Some samples had very high concentrations of cytokines, while others had no measurable cytokine elements. This heterogeneity was observed across all types of cytokines assessed.
- Fascinatingly, a significant connection was discovered between high cytokine concentrations in blood products and abnormal blood chemistry findings. In other words, horses with abnormal blood chemistry tended to have blood products with higher cytokine concentrations compared to horses with normal chemistry results.
Implications of the Findings
- Given the role of cytokines in causing inflammation and in regulating immune responses, the different concentrations in individual horses could have important implications when using PL for therapy and cell culture.
- The therapeutic effectiveness and how PL works could alter depending on the levels of pro-inflammatory and anti-inflammatory cytokines. This necessitates examining and understanding the cytokine content of these blood products before they are used therapeutically.
- Furthermore, blood chemistry may be a useful indicator to predict cytokine concentrations in blood products. This could offer an additional method for clinicians to tailor their treatment plans more effectively.
Cite This Article
APA
Moellerberndt J, Hagen A, Niebert S, Bu00fcttner K, Burk J.
(2023).
Cytokines in equine platelet lysate and related blood products.
Front Vet Sci, 10, 1117829.
https://doi.org/10.3389/fvets.2023.1117829 Publication
Researcher Affiliations
- Equine Clinic (Surgery, Orthopedics), Justus-Liebig-University Giessen, Giessen, Germany.
- Equine Clinic (Surgery, Orthopedics), Justus-Liebig-University Giessen, Giessen, Germany.
- Equine Clinic (Surgery, Orthopedics), Justus-Liebig-University Giessen, Giessen, Germany.
- Unit for Biomathematics and Data Processing, Faculty of Veterinary Medicine, Justus-Liebig-University Giessen, Giessen, Germany.
- Equine Clinic (Surgery, Orthopedics), Justus-Liebig-University Giessen, Giessen, Germany.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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