D-glucose transport and glycolytic enzyme activities in erythrocytes of dogs, pigs, cats, horses, cattle and sheep.
Abstract: The activities of D-glucose transport (D-GT) and the glycolytic enzymes hexokinase (HK) and pyruvate kinase (PK), were measured in the erythrocytes of dogs, pigs, cats, horses, cattle and sheep. The erythrocytes of dogs had the highest activities of D-GT, HK and PK, significantly higher than the activities in the erythrocytes of the herbivores. The activities of D-GT and HK in cat erythrocytes were significantly lower than in those of dogs. The differences between the activities of D-GT in the erythrocytes of the different species followed the differences in activities of HK but not those in the activities of PK or in the blood glucose concentrations. It is considered that the activity of HK provides a convenient measurement of the relative rates of glucose oxidation in erythrocytes.
Publication Date: 1995-03-01 PubMed ID: 7761703DOI: 10.1016/0034-5288(95)90078-0Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research article investigates the activities of D-glucose transport and two glycolytic enzymes (hexokinase and pyruvate kinase) in the red blood cells of various animals, finding significant differences between species and suggesting that hexokinase activity may be a useful measure of glucose oxidation rates in the cells.
Experiment Procedures and Comparisons Made
- The researchers measured the activities of D-glucose transport (D-GT) – the process by which glucose moves into cells – and two glycolytic enzymes crucial to glucose metabolism (hexokinase, HK, and pyruvate kinase, PK) in the red blood cells (erythrocytes) of dogs, pigs, cats, horses, cattle, and sheep.
- Comparisons were made between the levels of these activities in different animals, focusing especially on differences between carnivores (dogs and cats) and herbivores (pigs, horses, cattle, and sheep).
Main Findings of the Research
- The researchers found the highest activities of D-GT, HK, and PK in the erythrocytes of dogs, significantly higher than those found in the red blood cells of the studied herbivores.
- Erythrocytes of cats exhibited significantly lower activities of D-GT and HK than those of dogs, indicating differences even among different species of carnivores.
- Differences in the activities of D-GT in erythrocytes of different species seemed to follow those of HK. This relationship was not observed to the same extent with PK or with blood glucose concentrations.
Implications and Conclusions of the Study
- This study points to significant differences in glucose handling and metabolism at the cellular level across different animal species. More specifically, it highlights the differences between carnivorous and herbivorous species.
- The researchers proposed that the activity of hexokinase (HK) offers a convenient measurement of the relative rates of glucose oxidation in erythrocytes. This suggests that HK activity could serve as a novel biomarker for studying and comparing glucose metabolism across different species.
Cite This Article
APA
Arai T, Washizu T, Sagara M, Sako T, Nigi H, Matsumoto H, Sasaki M, Tomoda I.
(1995).
D-glucose transport and glycolytic enzyme activities in erythrocytes of dogs, pigs, cats, horses, cattle and sheep.
Res Vet Sci, 58(2), 195-196.
https://doi.org/10.1016/0034-5288(95)90078-0 Publication
Researcher Affiliations
- Department of Veterinary Science, Nippon Veterinary and Animal Science University, Tokyo, Japan.
MeSH Terms
- Animals
- Carnivora / blood
- Cats / blood
- Cattle / blood
- Dogs / blood
- Erythrocytes / enzymology
- Erythrocytes / metabolism
- Glucose / metabolism
- Hexokinase / blood
- Horses / blood
- Pyruvate Kinase / blood
- Ruminants / blood
- Sheep / blood
- Swine / blood
Citations
This article has been cited 9 times.- Miglio A, Maslanka M, Di Tommaso M, Rocconi F, Nemkov T, Buehler PW, Antognoni MT, Spitalnik SL, D'Alessandro A. ZOOMICS : comparative metabolomics of red blood cells from dogs, cows, horses and donkeys during refrigerated storage for up to 42 days.. Blood Transfus 2023 Jul;21(4):314-326.
- Subramaniam M, Enns CB, Loewen ME. Sigmoidal kinetics define porcine intestinal segregation of electrogenic monosaccharide transport systems as having multiple transporter population involvement.. Physiol Rep 2019 May;7(9):e14090.
- Subramaniam M, Weber LP, Loewen ME. Intestinal electrogenic sodium-dependent glucose absorption in tilapia and trout reveal species differences in SLC5A-associated kinetic segmental segregation.. Am J Physiol Regul Integr Comp Physiol 2019 Mar 1;316(3):R222-R234.
- Lacombe VA. Expression and regulation of facilitative glucose transporters in equine insulin-sensitive tissue: from physiology to pathology.. ISRN Vet Sci 2014;2014:409547.
- Suska M, Skotnicka E. Changes in Adenylate Nucleotides Concentration and Na, K-ATPase Activities in Erythrocytes of Horses in Function of Breed and Sex.. Vet Med Int 2010;2010:987309.
- Muhlhausler BS, Duffield JA, Ozanne SE, Pilgrim C, Turner N, Morrison JL, McMillen IC. The transition from fetal growth restriction to accelerated postnatal growth: a potential role for insulin signalling in skeletal muscle.. J Physiol 2009 Sep 1;587(Pt 17):4199-211.
- Tanaka A, Urabe S, Takeguchi A, Mizutani H, Sako T, Imai S, Yoshimura I, Kimura N, Arai T. Comparison of activities of enzymes related to energy metabolism in peripheral leukocytes and livers between Holstein dairy cows and ICR mice.. Vet Res Commun 2006 Jan;30(1):29-38.
- Arai T, Hashimoto K, Muzutani H, Kawabata T, Sako T, Washizu T. A comparison of the plasma fructose concentrations in dogs and cats and changes in the fructose concentrations in dogs following intravenous administration of fructose.. Vet Res Commun 1999 Jun;23(4):203-9.
- Washizu T, Kuramoto E, Abe M, Sako T, Arai T. A comparison of the activities of certain enzymes related to energy metabolism in leukocytes in dogs and cats.. Vet Res Commun 1998 Apr;22(3):187-92.
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