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Molecular & cellular proteomics : MCP2010; 9(10); 2292-2305; doi: 10.1074/mcp.M110.001073

Deciphering membrane-associated molecular processes in target tissue of autoimmune uveitis by label-free quantitative mass spectrometry.

Abstract: Autoimmune uveitis is a blinding disease presenting with autoantibodies against eye-specific proteins as well as autoagressive T cells invading and attacking the immune-privileged target tissue retina. The molecular events enabling T cells to invade and attack the tissue have remained elusive. Changes in membrane protein expression patterns between diseased and healthy stages are especially interesting because initiating events of disease will most likely occur at membranes. Since disease progression is accompanied with a break-down of the blood-retinal barrier, serum-derived proteins mask the potential target tissue-related changes. To overcome this limitation, we used membrane-enriched fractions derived from retinas of the only available spontaneous animal model for the disease equine recurrent uveitis, and compared expression levels by a label-free LC-MSMS-based strategy to healthy control samples. We could readily identify a total of 893 equine proteins with 57% attributed to the Gene Ontology project term "membrane." Of these, 179 proteins were found differentially expressed in equine recurrent uveitis tissue. Pathway enrichment analyses indicated an increase in proteins related to antigen processing and presentation, TNF receptor signaling, integrin cell surface interactions and focal adhesions. Additionally, loss of retina-specific proteins reflecting decrease of vision was observed as well as an increase in Müller glial cell-specific proteins indicating glial reactivity. Selected protein candidates (caveolin 1, integrin alpha 1 and focal adhesion kinase) were validated by immunohistochemistry and tissue staining pattern pointed to a significant increase of these proteins at the level of the outer limiting membrane which is part of the outer blood-retinal barrier. Taken together, the membrane enrichment in combination with LC-MSMS-based label-free quantification greatly increased the sensitivity of the comparative tissue profiling and resulted in detection of novel molecular pathways related to equine recurrent uveitis.
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Publication Date: 2010-07-04 PubMed ID: 20601722PubMed Central: PMC2953921DOI: 10.1074/mcp.M110.001073Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't
  • Validation Study

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The paper discusses a study on the molecular processes in the tissue targeted by autoimmune uveitis, a blinding disease, using a label-free quantitative mass spectrometry method. The research focussed on understanding the changes in membrane protein expression patterns during the disease to provide insight into disease initiation processes.

Objective of the Research

The main objective of the research was to explore the molecular events that enable T cells to invade tissues and instigate autoimmune uveitis. The study particularly analysed the changes in membrane protein expression patterns between the stages of disease and health, citing that the initial events of the disease most likely occur at the membranes.

Research Methodology

  • The research overcame the limitation of serum-derived proteins masking potential tissue-related changes through the use of membrane-enriched fractions. These were derived from retinas of the only known spontaneous animal model for the disease, equine recurrent uveitis.
  • The expression levels in the derived fractions were then compared to healthy control samples using a label-free LC-MSMS-based strategy.

Research Outcomes

  • A total of 893 equine proteins were identified in the process. Of these, 57% of the proteins were connected to the Gene Ontology project term “membrane,” implying their broad involvement in cellular processes occurring at or in proximity to cell membranes.
  • Approximately 179 proteins were found to exhibit differential expressions in equine recurrent uveitis tissues.
  • Pathway enrichment analyses revealed an increase in proteins associated with antigen processing and presentation, TNF receptor signaling, integrin cell surface interactions, and focal adhesions.
  • A decrease of vision-indicating proteins reflective of the loss of retina-specific proteins, and an increase in Müller glial cell-specific proteins indicating heightened glial reactivity were also observed.

Validation of Findings

  • Selected protein candidates (caveolin 1, integrin alpha 1, and focal adhesion kinase) uptake and their expression were validated through immunohistochemistry and tissue staining.
  • An increase in these proteins was noted on the outer limiting membrane, part of the outer blood-retinal barrier.
  • The validation suggested that the adopted methodology of membrane enrichment combined with LC-MSMS-based label-free quantification, significantly increased the sensitivity of the comparative tissue profiling.

In conclusion, the study effectively used comparative tissue profiling and was successful in detecting new molecular pathways associated with equine recurrent uveitis.

Cite This Article

APA
Hauck SM, Dietter J, Kramer RL, Hofmaier F, Zipplies JK, Amann B, Feuchtinger A, Deeg CA, Ueffing M. (2010). Deciphering membrane-associated molecular processes in target tissue of autoimmune uveitis by label-free quantitative mass spectrometry. Mol Cell Proteomics, 9(10), 2292-2305. https://doi.org/10.1074/mcp.M110.001073

Publication

Molecular & cellular proteomics : MCP
ISSN: 1535-9484
NlmUniqueID: 101125647
Country: United States
Language: English
Volume: 9
Issue: 10
Pages: 2292-2305

Researcher Affiliations

Hauck, Stefanie M
  • Department of Protein Science, Helmholtz Zentrum Mùnchen - German Research Center for Environmental Health, Neuherberg, Germany. hauck@helmholtz-muenchen.de
Dietter, Johannes
    Kramer, Roxane L
      Hofmaier, Florian
        Zipplies, Johanna K
          Amann, Barbara
            Feuchtinger, Annette
              Deeg, Cornelia A
                Ueffing, Marius

                  MeSH Terms

                  • Animals
                  • Autoimmune Diseases / metabolism
                  • Chromatography, Liquid
                  • Disease Models, Animal
                  • Horses
                  • Immunohistochemistry
                  • Mass Spectrometry / methods
                  • Membrane Proteins / metabolism
                  • Protein Binding
                  • Tandem Mass Spectrometry
                  • Uveitis / metabolism

                  References

                  This article includes 37 references
                  1. Forrester JV. Uveitis: pathogenesis.. Lancet 338, 1498–1501.
                    pubmed: 1683927
                  2. Martin TM, Smith JR, Rosenbaum JT. Anterior uveitis: current concepts of pathogenesis and interactions with the spondyloarthropathies.. Curr. Opin. Rheumatol. 14, 337–341.
                    pubmed: 12118164
                  3. Deeg CA, Hauck SM, Amann B, Pompetzki D, Altmann F, Raith A, Schmalzl T, Stangassinger M, Ueffing M. Equine recurrent uveitis–a spontaneous horse model of uveitis.. Ophthalmic. Res. 40, 151–153.
                    pubmed: 18421230
                  4. Deeg CA. A proteomic approach for studying the pathogenesis of spontaneous equine recurrent uveitis (ERU).. Vet. Immunol. Immunopathol. 128, 132–136.
                    pubmed: 19026452
                  5. Hauck SM, Schoeffmann S, Amann B, Stangassinger M, Gerhards H, Ueffing M, Deeg CA. Retinal Mueller glial cells trigger the hallmark inflammatory process in autoimmune uveitis.. J. Proteome Res. 6, 2121–2131.
                    pubmed: 17444670
                  6. Zipplies JK, Hauck SM, Schoeffmann S, Amann B, Stangassinger M, Ueffing M, Deeg CA. Serum PEDF levels are decreased in a spontaneous animal model for human autoimmune uveitis.. J. Proteome Res. 8, 992–998.
                    pubmed: 19113885
                  7. Caspi RR. Th1 and Th2 responses in pathogenesis and regulation of experimental autoimmune uveoretinitis.. Int. Rev. Immunol. 21, 197–208.
                    pubmed: 12424843
                  8. Deeg CA, Kaspers B, Gerhards H, Thurau SR, Wollanke B, Wildner G. Immune responses to retinal autoantigens and peptides in equine recurrent uveitis.. Invest. Ophthalmol. Vis. Sci. 42, 393–398.
                    pubmed: 11157872
                  9. Nagaraj N, Lu A, Mann M, Wisniewski JR. Detergent-based but gel-free method allows identification of several hundred membrane proteins in single LC-MS runs.. J. Proteome Res. 7, 5028–5032.
                    pubmed: 18839980
                  10. Hauck SM, Gloeckner CJ, Harley ME, Schoeffmann S, Boldt K, Ekstrom PA, Ueffing M. Identification of paracrine neuroprotective candidate proteins by a functional assay-driven proteomics approach.. Mol. Cell. Proteomics 7, 1349–1361.
                    pubmed: 18436526
                  11. Olsen JV, de Godoy LM, Li G, Macek B, Mortensen P, Pesch R, Makarov A, Lange O, Horning S, Mann M. Parts per million mass accuracy on an Orbitrap mass spectrometer via lock mass injection into a C-trap.. Mol. Cell. Proteomics 4, 2010–2021.
                    pubmed: 16249172
                  12. Kamburov A, Wierling C, Lehrach H, Herwig R. ConsensusPathDB–a database for integrating human functional interaction networks.. Nucleic Acids Res. 37, D623–D628.
                    pmc: PMC2686562pubmed: 18940869
                  13. Maere S, Heymans K, Kuiper M. BiNGO: a Cytoscape plugin to assess overrepresentation of gene ontology categories in biological networks.. Bioinformatics 21, 3448–3449.
                    pubmed: 15972284
                  14. Krogh A, Larsson B, von Heijne G, Sonnhammer EL. Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.. J. Mol. Biol. 305, 567–580.
                    pubmed: 11152613
                  15. Jensen LJ, Kuhn M, Stark M, Chaffron S, Creevey C, Muller J, Doerks T, Julien P, Roth A, Simonovic M, Bork P, von Mering C. STRING 8–a global view on proteins and their functional interactions in 630 organisms.. Nucleic Acids Res. 37, D412–D416.
                    pmc: PMC2686466pubmed: 18940858
                  16. Ehrenhofer MC, Deeg CA, Reese S, Liebich HG, Stangassinger M, Kaspers B. Normal structure and age-related changes of the equine retina.. Vet. Ophthalmol. 5, 39–47.
                    pubmed: 11940247
                  17. Morgan JM, Navabi H, Schmid KW, Jasani B. Possible role of tissue-bound calcium ions in citrate-mediated high-temperature antigen retrieval.. J. Pathol. 174, 301–307.
                    pubmed: 7884592
                  18. Baatz M, Arini N, Schape A, Binnig G, Linssen B. Object-oriented image analysis for high content screening: detailed quantification of cells and sub cellular structures with the Cellenger software.. Cytometry A. 69, 652–658.
                    pubmed: 16680706
                  19. Baatz M, Zimmermann J, Blackmore CG. Automated analysis and detailed quantification of biomedical images using Definiens Cognition Network Technology.. Comb. Chem. High Throughput Screen 12, 908–916.
                    pubmed: 19531006
                  20. Albrethsen J, Knol JC, Piersma S, Pham TV, de Wit M, Mongera S, Carvalho B, Verheul HM, Fijneman RJ, Meijer GA, Jimenez CR. Sub-nuclear proteomics in colorectal cancer: Identification of proteins enriched in the nuclear matrix fraction and regulation in adenoma to carcinoma progression.. Mol. Cell. Proteomics 5, 988–1005.
                    pmc: PMC2871429pubmed: 20089989
                  21. Wade CM, Giulotto E, Sigurdsson S, Zoli M, Gnerre S, Imsland F, Lear TL, Adelson DL, Bailey E, Bellone RR, Blocker H, Distl O, Edgar RC, Garber M, Leeb T, Mauceli E, MacLeod JN, Penedo MC, Raison JM, Sharpe T, Vogel J, Andersson L, Antczak DF, Biagi T, Binns MM, Chowdhary BP, Coleman SJ, Della Valle G, Fryc S, Guerin G, Hasegawa T, Hill EW, Jurka J, Kiialainen A, Lindgren G, Liu J, Magnani E, Mickelson JR, Murray J, Nergadze SG, Onofrio R, Pedroni S, Piras MF, Raudsepp T, Rocchi M, Roed KH, Ryder OA, Searle S, Skow L, Swinburne JE, Syvanen AC, Tozaki T, Valberg SJ, Vaudin M, White JR, Zody MC, Lander ES, Lindblad-Toh K. Genome sequence, comparative analysis, and population genetics of the domestic horse.. Science 326, 865–867.
                    pmc: PMC3785132pubmed: 19892987
                  22. Hauck SM, Ekstrom PA, Ahuja-Jensen P, Suppmann S, Paquet-Durand F, van Veen T, Ueffing M. Differential modification of phosducin protein in degenerating rd1 retina is associated with constitutively active Ca2+/calmodulin kinase II in rod outer segments.. Mol. Cell. Proteomics 5, 324–336.
                    pubmed: 16253986
                  23. Iandiev I, Uckermann O, Pannicke T, Wurm A, Tenckhoff S, Pietsch UC, Reichenbach A, Wiedemann P, Bringmann A, Uhlmann S. Glial cell reactivity in a porcine model of retinal detachment.. Invest. Ophthalmol. Vis. Sci. 47, 2161–2171.
                    pubmed: 16639028
                  24. Iandiev I, Tenckhoff S, Pannicke T, Biedermann B, Hollborn M, Wiedemann P, Reichenbach A, Bringmann A. Differential regulation of Kir4.1 and Kir2.1 expression in the ischemic rat retina.. Neurosci. Lett. 396, 97–101.
                    pubmed: 16330144
                  25. Pannicke T, Uckermann O, Iandiev I, Wiedemann P, Reichenbach A, Bringmann A. Ocular inflammation alters swelling and membrane characteristics of rat Muller glial cells.. J. Neuroimmunol. 161, 145–154.
                    pubmed: 15748953
                  26. Punzo C, Kornacker K, Cepko CL. Stimulation of the insulin/mTOR pathway delays cone death in a mouse model of retinitis pigmentosa.. Nat. Neurosci. 12, 44–52.
                    pmc: PMC3339764pubmed: 19060896
                  27. Cresswell P, Ackerman AL, Giodini A, Peaper DR, Wearsch PA. Mechanisms of MHC class I-restricted antigen processing and cross-presentation.. Immunol. Rev. 207, 145–157.
                    pubmed: 16181333
                  28. Epperson DE, Arnold D, Spies T, Cresswell P, Pober JS, Johnson DR. Cytokines increase transporter in antigen processing-1 expression more rapidly than HLA class I expression in endothelial cells.. J. Immunol. 149, 3297–3301.
                    pubmed: 1385520
                  29. Ma W, Lehner PJ, Cresswell P, Pober JS, Johnson DR. Interferon-gamma rapidly increases peptide transporter (TAP) subunit expression and peptide transport capacity in endothelial cells.. J. Biol. Chem. 272, 16585–16590.
                    pubmed: 9195970
                  30. Gosens I, den Hollander AI, Cremers FP, Roepman R. Composition and function of the Crumbs protein complex in the mammalian retina.. Exp. Eye Res. 86, 713–726.
                    pubmed: 18407265
                  31. Crane IJ, Liversidge J. Mechanisms of leukocyte migration across the blood-retina barrier.. Semin. Immunopathol. 30, 165–177.
                    pmc: PMC2315689pubmed: 18305941
                  32. Sheppard D. Integrin-mediated activation of latent transforming growth factor beta.. Cancer Metastasis Rev. 24, 395–402.
                    pubmed: 16258727
                  33. Ohta K, Wiggert B, Yamagami S, Taylor AW, Streilein JW. Analysis of immunomodulatory activities of aqueous humor from eyes of mice with experimental autoimmune uveitis.. J. Immunol. 164, 1185–1192.
                    pubmed: 10640729
                  34. Vinores SA, Chan CC, Vinores MA, Matteson DM, Chen YS, Klein DA, Shi A, Ozaki H, Campochiaro PA. Increased vascular endothelial growth factor (VEGF) and transforming growth factor beta (TGFbeta) in experimental autoimmune uveoretinitis: upregulation of VEGF without neovascularization.. J. Neuroimmunol. 89, 43–50.
                    pubmed: 9726824
                  35. Wijelath ES, Rahman S, Namekata M, Murray J, Nishimura T, Mostafavi-Pour Z, Patel Y, Suda Y, Humphries MJ, Sobel M. Heparin-II domain of fibronectin is a vascular endothelial growth factor-binding domain: enhancement of VEGF biological activity by a singular growth factor/matrix protein synergism.. Circ. Res. 99, 853–860.
                    pmc: PMC3175430pubmed: 17008606
                  36. Wijelath ES, Murray J, Rahman S, Patel Y, Ishida A, Strand K, Aziz S, Cardona C, Hammond WP, Savidge GF, Rafii S, Sobel M. Novel vascular endothelial growth factor binding domains of fibronectin enhance vascular endothelial growth factor biological activity.. Circ. Res. 91, 25–31.
                    pubmed: 12114318
                  37. Deeg CA, Altmann F, Hauck SM, Schoeffmann S, Amann B, Stangassinger M, Ueffing M. Down-regulation of pigment epithelium-derived factor in uveitic lesion associates with focal vascular endothelial growth factor expression and breakdown of the blood-retinal barrier.. Proteomics 7, 1540–1548.
                    pubmed: 17407186

                  Citations

                  This article has been cited 90 times.
                  1. Chakraborty A, Giraldo-Arias J, Merl-Pham J, Dick E, Mastalerz M, Zöller M, Marchi H, Le Gleut R, Hatz RA, Behr J, Hilgendorff A, Hauck SM, Staab-Weijnitz CA. Cholesterol Regulates Airway Epithelial Cell Differentiation by Inhibiting p53 Nuclear Translocation. Int J Mol Sci 2025 Aug 27;26(17).
                    doi: 10.3390/ijms26178324pubmed: 40943244google scholar: lookup
                  2. Gao Y, Mi L, Deng Y, Jia Z, Zhao M, Hu Y, Ji Y, He X, Xu K. Revolutionizing Autoimmune Research: The Role of Caveolin-1. Immun Inflamm Dis 2025 Aug;13(8):e70230.
                    doi: 10.1002/iid3.70230pubmed: 40778471google scholar: lookup
                  3. Maddhesiya P, Lepko T, Steiner-Mezzardi A, Schneider J, Schwarz V, Merl-Pham J, Berger F, Hauck SM, Ronfani L, Bianchi M, Simon T, Krontira A, Masserdotti G, Götz M, Ninkovic J. Hmgb2 improves astrocyte to neuron conversion by increasing the chromatin accessibility of genes associated with neuronal maturation in a proneuronal factor-dependent manner. Genome Biol 2025 Apr 17;26(1):100.
                    doi: 10.1186/s13059-025-03556-zpubmed: 40247387google scholar: lookup
                  4. Lourbopoulos A, Müller SA, Jocher G, Wick M, Plesnila N, Lichtenthaler SF. An Improved Method for Sampling and Quantitative Protein Analytics of Cerebrospinal Fluid of Individual Mice. Mol Cell Proteomics 2025 May;24(5):100958.
                    doi: 10.1016/j.mcpro.2025.100958pubmed: 40157722google scholar: lookup
                  5. Kellner M, Hörmann J, Fackler S, Hu Y, Zhou T, Lu L, Ilik I, Aktas T, Feederle R, Hauck SM, Gires O, Gärtner K, Li L, Zeidler R. The Nuclear Speckles Protein SRRM2 Is Exposed on the Surface of Cancer Cells. Cells 2024 Sep 17;13(18).
                    doi: 10.3390/cells13181563pubmed: 39329747google scholar: lookup
                  6. Qin W, Liang A, Han X, Zhang M, Gao Y, Zhao C. Quantitative urinary proteome analysis reveals potential biomarkers for disease activity of Behcet's disease uveitis. BMC Ophthalmol 2024 Jul 9;24(1):277.
                    doi: 10.1186/s12886-024-03557-9pubmed: 38982370google scholar: lookup
                  7. Peng H, Xin S, Pfeiffer S, Müller C, Merl-Pham J, Hauck SM, Harter PN, Spitzer D, Devraj K, Varynskyi B, Arzberger T, Momma S, Schick JA. Fatty acid-binding protein 5 is a functional biomarker and indicator of ferroptosis in cerebral hypoxia. Cell Death Dis 2024 Apr 23;15(4):286.
                    doi: 10.1038/s41419-024-06681-ypubmed: 38653992google scholar: lookup
                  8. Abbasi M, Gupta V, Chitranshi N, Moustardas P, Ranjbaran R, Graham SL. Molecular Mechanisms of Glaucoma Pathogenesis with Implications to Caveolin Adaptor Protein and Caveolin-Shp2 Axis. Aging Dis 2024 Oct 1;15(5):2051-2068.
                    doi: 10.14336/AD.2023.1012pubmed: 37962455google scholar: lookup
                  9. Azimzadeh O, Merl-Pham J, Subramanian V, Oleksenko K, Krumm F, Mancuso M, Pasquali E, Tanaka IB 3rd, Tanaka S, Atkinson MJ, Tapio S, Moertl S. Late Effects of Chronic Low Dose Rate Total Body Irradiation on the Heart Proteome of ApoE(-/-) Mice Resemble Premature Cardiac Ageing. Cancers (Basel) 2023 Jun 29;15(13).
                    doi: 10.3390/cancers15133417pubmed: 37444528google scholar: lookup
                  10. Enyong EN, Gurley J, Sjoelung V, Elliott MH. Caveolin-1 in Müller Glia Exists as Heat-Resistant, High Molecular Weight Complexes. Adv Exp Med Biol 2023;1415:249-256.
                    doi: 10.1007/978-3-031-27681-1_36pubmed: 37440041google scholar: lookup
                  11. Brett E, Rosemann M, Azimzadeh O, Pagani A, Prahm C, Daigeler A, Duscher D, Kolbenschlag J. Irradiated Triple-Negative Breast Cancer Co-Culture Produces a Less Oncogenic Extracellular Matrix. Int J Mol Sci 2022 Jul 27;23(15).
                    doi: 10.3390/ijms23158265pubmed: 35897841google scholar: lookup
                  12. Prokai L, Zaman K, Prokai-Tatrai K. Mass spectrometry-based retina proteomics. Mass Spectrom Rev 2023 May;42(3):1032-1062.
                    doi: 10.1002/mas.21786pubmed: 35670041google scholar: lookup
                  13. Lam DD, Williams RH, Lujan E, Tanabe K, Huber G, Saw NL, Merl-Pham J, Salminen AV, Lohse D, Spendiff S, Plastini MJ, Zech M, Lochmüller H, Geerlof A, Hauck SM, Shamloo M, Wernig M, Winkelmann J. Collagen VI Regulates Motor Circuit Plasticity and Motor Performance by Cannabinoid Modulation. J Neurosci 2022 Feb 23;42(8):1557-1573.
                    doi: 10.1523/JNEUROSCI.0962-21.2021pubmed: 34965974google scholar: lookup
                  14. Azimzadeh O, Subramanian V, Sievert W, Merl-Pham J, Oleksenko K, Rosemann M, Multhoff G, Atkinson MJ, Tapio S. Activation of PPARα by Fenofibrate Attenuates the Effect of Local Heart High Dose Irradiation on the Mouse Cardiac Proteome. Biomedicines 2021 Dec 6;9(12).
                    doi: 10.3390/biomedicines9121845pubmed: 34944662google scholar: lookup
                  15. Gerckens M, Schorpp K, Pelizza F, Wögrath M, Reichau K, Ma H, Dworsky AM, Sengupta A, Stoleriu MG, Heinzelmann K, Merl-Pham J, Irmler M, Alsafadi HN, Trenkenschuh E, Sarnova L, Jirouskova M, Frieß W, Hauck SM, Beckers J, Kneidinger N, Behr J, Hilgendorff A, Hadian K, Lindner M, Königshoff M, Eickelberg O, Gregor M, Plettenburg O, Yildirim AÖ, Burgstaller G. Phenotypic drug screening in a human fibrosis model identified a novel class of antifibrotic therapeutics. Sci Adv 2021 Dec 24;7(52):eabb3673.
                    doi: 10.1126/sciadv.abb3673pubmed: 34936468google scholar: lookup
                  16. Xin S, Mueller C, Pfeiffer S, Kraft VAN, Merl-Pham J, Bao X, Feederle R, Jin X, Hauck SM, Schmitt-Kopplin P, Schick JA. MS4A15 drives ferroptosis resistance through calcium-restricted lipid remodeling. Cell Death Differ 2022 Mar;29(3):670-686.
                    doi: 10.1038/s41418-021-00883-zpubmed: 34663908google scholar: lookup
                  17. Jin X, Zhang Y, Zhang X, Li Y, Xu M, Liu K, Ru J, Ma C, Yao Y, He Y, Gao J. An Adipose-Derived Injectable Sustained-Release Collagen Scaffold of Adipokines Prepared Through a Fast Mechanical Processing Technique for Preventing Skin Photoaging in Mice. Front Cell Dev Biol 2021;9:722427.
                    doi: 10.3389/fcell.2021.722427pubmed: 34631708google scholar: lookup
                  18. Jing J, Du Z, Qin W. Proteome Analysis of Urinary Biomarkers in Acute Hypercoagulable State Rat Model. Front Mol Biosci 2021;8:634606.
                    doi: 10.3389/fmolb.2021.634606pubmed: 33996895google scholar: lookup
                  19. Lorenz L, Hirmer S, Schmalen A, Hauck SM, Deeg CA. Cell Surface Profiling of Retinal Müller Glial Cells Reveals Association to Immune Pathways after LPS Stimulation. Cells 2021 Mar 23;10(3).
                    doi: 10.3390/cells10030711pubmed: 33806940google scholar: lookup
                  20. Merl-Pham J, Basak T, Knüppel L, Ramanujam D, Athanason M, Behr J, Engelhardt S, Eickelberg O, Hauck SM, Vanacore R, Staab-Weijnitz CA. Quantitative proteomic profiling of extracellular matrix and site-specific collagen post-translational modifications in an in vitro model of lung fibrosis. Matrix Biol Plus 2019 Feb;1:100005.
                    doi: 10.1016/j.mbplus.2019.04.002pubmed: 33543004google scholar: lookup
                  21. Degroote RL, Deeg CA. Immunological Insights in Equine Recurrent Uveitis. Front Immunol 2020;11:609855.
                    doi: 10.3389/fimmu.2020.609855pubmed: 33488614google scholar: lookup
                  22. Zhao L, Cui Y, Fu G, Xu Z, Liao X, Zhang D. Signal Recognition Particle Suppressor Screening Reveals the Regulation of Membrane Protein Targeting by the Translation Rate. mBio 2021 Jan 12;12(1).
                    doi: 10.1128/mBio.02373-20pubmed: 33436432google scholar: lookup
                  23. Gerhards R, Pfeffer LK, Lorenz J, Starost L, Nowack L, Thaler FS, Schlüter M, Rübsamen H, Macrini C, Winklmeier S, Mader S, Bronge M, Grönlund H, Feederle R, Hsia HE, Lichtenthaler SF, Merl-Pham J, Hauck SM, Kuhlmann T, Bauer IJ, Beltran E, Gerdes LA, Mezydlo A, Bar-Or A, Banwell B, Khademi M, Olsson T, Hohlfeld R, Lassmann H, Kümpfel T, Kawakami N, Meinl E. Oligodendrocyte myelin glycoprotein as a novel target for pathogenic autoimmunity in the CNS. Acta Neuropathol Commun 2020 Nov 30;8(1):207.
                    doi: 10.1186/s40478-020-01086-2pubmed: 33256847google scholar: lookup
                  24. Yu M, Xie F, Liu X, Sun H, Guo Z, Liu X, Li W, Sun W, Wang Y, He C. Proteomic Study of Aqueous Humor and Its Application in the Treatment of Neovascular Glaucoma. Front Mol Biosci 2020;7:587677.
                    doi: 10.3389/fmolb.2020.587677pubmed: 33195434google scholar: lookup
                  25. Gurley JM, Gmyrek GB, McClellan ME, Hargis EA, Hauck SM, Dozmorov MG, Wren JD, Carr DJJ, Elliott MH. Neuroretinal-Derived Caveolin-1 Promotes Endotoxin-Induced Inflammation in the Murine Retina. Invest Ophthalmol Vis Sci 2020 Oct 1;61(12):19.
                    doi: 10.1167/iovs.61.12.19pubmed: 33079993google scholar: lookup
                  26. Raguz N, Heim A, Engal E, Wesche J, Merl-Pham J, Hauck SM, Erkelenz S, Schaal H, Bensaude O, Wolf A, Salton M, Böttger A. JMJD6 Regulates Splicing of Its Own Gene Resulting in Alternatively Spliced Isoforms with Different Nuclear Targets. Int J Mol Sci 2020 Sep 10;21(18).
                    doi: 10.3390/ijms21186618pubmed: 32927736google scholar: lookup
                  27. Marozava S, Merl-Pham J, Müller H, Meckenstock RU. Adaptation of Carbon Source Utilization Patterns of Geobacter metallireducens During Sessile Growth. Front Microbiol 2020;11:1271.
                    doi: 10.3389/fmicb.2020.01271pubmed: 32655526google scholar: lookup
                  28. Abbasi M, Gupta VK, Chitranshi N, Gupta VB, Mirzaei M, Dheer Y, Garthwaite L, Zaw T, Parton RG, You Y, Graham SL. Caveolin-1 Ablation Imparts Partial Protection Against Inner Retinal Injury in Experimental Glaucoma and Reduces Apoptotic Activation. Mol Neurobiol 2020 Sep;57(9):3759-3784.
                    doi: 10.1007/s12035-020-01948-9pubmed: 32578008google scholar: lookup
                  29. Brett E, Sauter M, Timmins É, Azimzadeh O, Rosemann M, Merl-Pham J, Hauck SM, Nelson PJ, Becker KF, Schunn I, Lowery A, Kerin MJ, Atkinson M, Krüger A, Machens HG, Duscher D. Oncogenic Linear Collagen VI of Invasive Breast Cancer Is Induced by CCL5. J Clin Med 2020 Apr 2;9(4).
                    doi: 10.3390/jcm9040991pubmed: 32252260google scholar: lookup
                  30. Moertl S, Buschmann D, Azimzadeh O, Schneider M, Kell R, Winkler K, Tapio S, Hornhardt S, Merl-Pham J, Pfaffl MW, Atkinson MJ. Radiation Exposure of Peripheral Mononuclear Blood Cells Alters the Composition and Function of Secreted Extracellular Vesicles. Int J Mol Sci 2020 Mar 27;21(7).
                    doi: 10.3390/ijms21072336pubmed: 32230970google scholar: lookup
                  31. Monson RK, Winkler B, Rosenstiel TN, Block K, Merl-Pham J, Strauss SH, Ault K, Maxfield J, Moore DJP, Trahan NA, Neice AA, Shiach I, Barron-Gafford GA, Ibsen P, McCorkel JT, Bernhardt J, Schnitzler JP. High productivity in hybrid-poplar plantations without isoprene emission to the atmosphere. Proc Natl Acad Sci U S A 2020 Jan 21;117(3):1596-1605.
                    doi: 10.1073/pnas.1912327117pubmed: 31907313google scholar: lookup
                  32. Wei J, Ni N, Meng W, Gao Y. Early urine proteome changes in the Walker-256 tail-vein injection rat model. Sci Rep 2019 Sep 24;9(1):13804.
                    doi: 10.1038/s41598-019-50301-1pubmed: 31551472google scholar: lookup
                  33. He Y, Xia J, Chen H, Wang L, Deng C, Lu F. Human adipose liquid extract induces angiogenesis and adipogenesis: a novel cell-free therapeutic agent. Stem Cell Res Ther 2019 Aug 14;10(1):252.
                    doi: 10.1186/s13287-019-1356-0pubmed: 31412933google scholar: lookup
                  34. Zhang F, Li X, Ni Y, Shan G, Gao Y. Preliminary study of the urinary proteome in Li and Han ethnic individuals from Hainan. Sci China Life Sci 2020 Jan;63(1):125-137.
                    doi: 10.1007/s11427-018-9485-4pubmed: 31102176google scholar: lookup
                  35. Mages K, Grassmann F, Jägle H, Rupprecht R, Weber BHF, Hauck SM, Grosche A. The agonistic TSPO ligand XBD173 attenuates the glial response thereby protecting inner retinal neurons in a murine model of retinal ischemia. J Neuroinflammation 2019 Feb 18;16(1):43.
                    doi: 10.1186/s12974-019-1424-5pubmed: 30777091google scholar: lookup
                  36. Turapov O, Forti F, Kadhim B, Ghisotti D, Sassine J, Straatman-Iwanowska A, Bottrill AR, Moynihan PJ, Wallis R, Barthe P, Cohen-Gonsaud M, Ajuh P, Vollmer W, Mukamolova GV. Two Faces of CwlM, an Essential PknB Substrate, in Mycobacterium tuberculosis. Cell Rep 2018 Oct 2;25(1):57-67.e5.
                    doi: 10.1016/j.celrep.2018.09.004pubmed: 30282038google scholar: lookup
                  37. Schauer M, Kleinwort KJH, Degroote RL, Wiedemann C, Kremmer E, Hauck SM, Deeg CA. Interaction of septin 7 and DOCK8 in equine lymphocytes reveals novel insights into signaling pathways associated with autoimmunity. Sci Rep 2018 Aug 17;8(1):12332.
                    doi: 10.1038/s41598-018-30753-7pubmed: 30120291google scholar: lookup
                  38. Einer C, Hohenester S, Wimmer R, Wottke L, Artmann R, Schulz S, Gosmann C, Simmons A, Leitzinger C, Eberhagen C, Borchard S, Schmitt S, Hauck SM, von Toerne C, Jastroch M, Walheim E, Rust C, Gerbes AL, Popper B, Mayr D, Schnurr M, Vollmar AM, Denk G, Zischka H. Data on chow, liver tissue and mitochondrial fatty acid compositions as well as mitochondrial proteome changes after feeding mice a western diet for 6-24 weeks. Data Brief 2017 Dec;15:163-169.
                    doi: 10.1016/j.dib.2017.09.019pubmed: 29034285google scholar: lookup
                  39. Wang C, Huang Z, Yu K, Ding R, Ye K, Dai C, Xu X, Zhou G, Li C. High-Salt Diet Has a Certain Impact on Protein Digestion and Gut Microbiota: A Sequencing and Proteome Combined Study. Front Microbiol 2017;8:1838.
                    doi: 10.3389/fmicb.2017.01838pubmed: 29033907google scholar: lookup
                  40. Baselet B, Azimzadeh O, Erbeldinger N, Bakshi MV, Dettmering T, Janssen A, Ktitareva S, Lowe DJ, Michaux A, Quintens R, Raj K, Durante M, Fournier C, Benotmane MA, Baatout S, Sonveaux P, Tapio S, Aerts A. Differential Impact of Single-Dose Fe Ion and X-Ray Irradiation on Endothelial Cell Transcriptomic and Proteomic Responses. Front Pharmacol 2017;8:570.
                    doi: 10.3389/fphar.2017.00570pubmed: 28993729google scholar: lookup
                  41. Zhao M, Li M, Yang Y, Guo Z, Sun Y, Shao C, Li M, Sun W, Gao Y. A comprehensive analysis and annotation of human normal urinary proteome. Sci Rep 2017 Jun 8;7(1):3024.
                    doi: 10.1038/s41598-017-03226-6pubmed: 28596590google scholar: lookup
                  42. Obermann J, Priglinger CS, Merl-Pham J, Geerlof A, Priglinger S, Götz M, Hauck SM. Proteome-wide Identification of Glycosylation-dependent Interactors of Galectin-1 and Galectin-3 on Mesenchymal Retinal Pigment Epithelial (RPE) Cells. Mol Cell Proteomics 2017 Aug;16(8):1528-1546.
                    doi: 10.1074/mcp.M116.066381pubmed: 28576849google scholar: lookup
                  43. Cehofski LJ, Honoré B, Vorum H. A Review: Proteomics in Retinal Artery Occlusion, Retinal Vein Occlusion, Diabetic Retinopathy and Acquired Macular Disorders. Int J Mol Sci 2017 Apr 28;18(5).
                    doi: 10.3390/ijms18050907pubmed: 28452939google scholar: lookup
                  44. Lee H, Qian K, von Toerne C, Hoerburger L, Claussnitzer M, Hoffmann C, Glunk V, Wahl S, Breier M, Eck F, Jafari L, Molnos S, Grallert H, Dahlman I, Arner P, Brunner C, Hauner H, Hauck SM, Laumen H. Allele-specific quantitative proteomics unravels molecular mechanisms modulated by cis-regulatory PPARG locus variation. Nucleic Acids Res 2017 Apr 7;45(6):3266-3279.
                    doi: 10.1093/nar/gkx105pubmed: 28334807google scholar: lookup
                  45. Schäfer N, Grosche A, Reinders J, Hauck SM, Pouw RB, Kuijpers TW, Wouters D, Ehrenstein B, Enzmann V, Zipfel PF, Skerka C, Pauly D. Complement Regulator FHR-3 Is Elevated either Locally or Systemically in a Selection of Autoimmune Diseases. Front Immunol 2016;7:542.
                    doi: 10.3389/fimmu.2016.00542pubmed: 27965669google scholar: lookup
                  46. von Toerne C, Laimighofer M, Achenbach P, Beyerlein A, de Las Heras Gala T, Krumsiek J, Theis FJ, Ziegler AG, Hauck SM. Peptide serum markers in islet autoantibody-positive children. Diabetologia 2017 Feb;60(2):287-295.
                    doi: 10.1007/s00125-016-4150-xpubmed: 27815605google scholar: lookup
                  47. Gu X, Reagan AM, McClellan ME, Elliott MH. Caveolins and caveolae in ocular physiology and pathophysiology. Prog Retin Eye Res 2017 Jan;56:84-106.
                    doi: 10.1016/j.preteyeres.2016.09.005pubmed: 27664379google scholar: lookup
                  48. Palfi A, Hokamp K, Hauck SM, Vencken S, Millington-Ward S, Chadderton N, Carrigan M, Kortvely E, Greene CM, Kenna PF, Farrar GJ. microRNA regulatory circuits in a mouse model of inherited retinal degeneration. Sci Rep 2016 Aug 16;6:31431.
                    doi: 10.1038/srep31431pubmed: 27527066google scholar: lookup
                  49. Amann B, Kleinwort KJ, Hirmer S, Sekundo W, Kremmer E, Hauck SM, Deeg CA. Expression and Distribution Pattern of Aquaporin 4, 5 and 11 in Retinas of 15 Different Species. Int J Mol Sci 2016 Jul 16;17(7).
                    doi: 10.3390/ijms17071145pubmed: 27438827google scholar: lookup
                  50. Azimzadeh O, Azizova T, Merl-Pham J, Subramanian V, Bakshi MV, Moseeva M, Zubkova O, Hauck SM, Anastasov N, Atkinson MJ, Tapio S. A dose-dependent perturbation in cardiac energy metabolism is linked to radiation-induced ischemic heart disease in Mayak nuclear workers. Oncotarget 2017 Feb 7;8(6):9067-9078.
                    doi: 10.18632/oncotarget.10424pubmed: 27391067google scholar: lookup
                  51. Huber MC, Falkenberg N, Hauck SM, Priller M, Braselmann H, Feuchtinger A, Walch A, Schmitt M, Aubele M. Cyr61 and YB-1 are novel interacting partners of uPAR and elevate the malignancy of triple-negative breast cancer. Oncotarget 2016 Jul 12;7(28):44062-44075.
                    doi: 10.18632/oncotarget.9853pubmed: 27286449google scholar: lookup
                  52. Bakshi MV, Azimzadeh O, Merl-Pham J, Verreet T, Hauck SM, Benotmane MA, Atkinson MJ, Tapio S. In-Utero Low-Dose Irradiation Leads to Persistent Alterations in the Mouse Heart Proteome. PLoS One 2016;11(6):e0156952.
                    doi: 10.1371/journal.pone.0156952pubmed: 27276052google scholar: lookup
                  53. Carella P, Merl-Pham J, Wilson DC, Dey S, Hauck SM, Vlot AC, Cameron RK. Comparative Proteomics Analysis of Phloem Exudates Collected during the Induction of Systemic Acquired Resistance. Plant Physiol 2016 Jun;171(2):1495-510.
                    doi: 10.1104/pp.16.00269pubmed: 27208255google scholar: lookup
                  54. Deeg CA, Amann B, Lutz K, Hirmer S, Lutterberg K, Kremmer E, Hauck SM. Aquaporin 11, a regulator of water efflux at retinal Müller glial cell surface decreases concomitant with immune-mediated gliosis. J Neuroinflammation 2016 Apr 23;13(1):89.
                    doi: 10.1186/s12974-016-0554-2pubmed: 27107718google scholar: lookup
                  55. Vanzo E, Merl-Pham J, Velikova V, Ghirardo A, Lindermayr C, Hauck SM, Bernhardt J, Riedel K, Durner J, Schnitzler JP. Modulation of Protein S-Nitrosylation by Isoprene Emission in Poplar. Plant Physiol 2016 Apr;170(4):1945-61.
                    doi: 10.1104/pp.15.01842pubmed: 26850277google scholar: lookup
                  56. Grosche A, Hauser A, Lepper MF, Mayo R, von Toerne C, Merl-Pham J, Hauck SM. The Proteome of Native Adult Müller Glial Cells From Murine Retina. Mol Cell Proteomics 2016 Feb;15(2):462-80.
                    doi: 10.1074/mcp.M115.052183pubmed: 26324419google scholar: lookup
                  57. Schäfer A, Neschen S, Kahle M, Sarioglu H, Gaisbauer T, Imhof A, Adamski J, Hauck SM, Ueffing M. The Epoxyeicosatrienoic Acid Pathway Enhances Hepatic Insulin Signaling and is Repressed in Insulin-Resistant Mouse Liver. Mol Cell Proteomics 2015 Oct;14(10):2764-74.
                    doi: 10.1074/mcp.M115.049064pubmed: 26070664google scholar: lookup
                  58. Laurent SA, Hoffmann FS, Kuhn PH, Cheng Q, Chu Y, Schmidt-Supprian M, Hauck SM, Schuh E, Krumbholz M, Rübsamen H, Wanngren J, Khademi M, Olsson T, Alexander T, Hiepe F, Pfister HW, Weber F, Jenne D, Wekerle H, Hohlfeld R, Lichtenthaler SF, Meinl E. γ-Secretase directly sheds the survival receptor BCMA from plasma cells. Nat Commun 2015 Jun 11;6:7333.
                    doi: 10.1038/ncomms8333pubmed: 26065893google scholar: lookup
                  59. Graessel A, Hauck SM, von Toerne C, Kloppmann E, Goldberg T, Koppensteiner H, Schindler M, Knapp B, Krause L, Dietz K, Schmidt-Weber CB, Suttner K. A Combined Omics Approach to Generate the Surface Atlas of Human Naive CD4+ T Cells during Early T-Cell Receptor Activation. Mol Cell Proteomics 2015 Aug;14(8):2085-102.
                    doi: 10.1074/mcp.M114.045690pubmed: 25991687google scholar: lookup
                  60. Barjaktarovic Z, Kempf SJ, Sriharshan A, Merl-Pham J, Atkinson MJ, Tapio S. Ionizing radiation induces immediate protein acetylation changes in human cardiac microvascular endothelial cells. J Radiat Res 2015 Jul;56(4):623-32.
                    doi: 10.1093/jrr/rrv014pubmed: 25840449google scholar: lookup
                  61. Zhao M, Li X, Li M, Gao Y. Effects of anesthetics pentobarbital sodium and chloral hydrate on urine proteome. PeerJ 2015;3:e813.
                    doi: 10.7717/peerj.813pubmed: 25789206google scholar: lookup
                  62. Wildburger NC, Ali SR, Hsu WC, Shavkunov AS, Nenov MN, Lichti CF, LeDuc RD, Mostovenko E, Panova-Elektronova NI, Emmett MR, Nilsson CL, Laezza F. Quantitative proteomics reveals protein-protein interactions with fibroblast growth factor 12 as a component of the voltage-gated sodium channel 1.2 (nav1.2) macromolecular complex in Mammalian brain. Mol Cell Proteomics 2015 May;14(5):1288-300.
                    doi: 10.1074/mcp.M114.040055pubmed: 25724910google scholar: lookup
                  63. Kahle M, Schäfer A, Seelig A, Schultheiß J, Wu M, Aichler M, Leonhardt J, Rathkolb B, Rozman J, Sarioglu H, Hauck SM, Ueffing M, Wolf E, Kastenmueller G, Adamski J, Walch A, Hrabé de Angelis M, Neschen S. High fat diet-induced modifications in membrane lipid and mitochondrial-membrane protein signatures precede the development of hepatic insulin resistance in mice. Mol Metab 2015 Jan;4(1):39-50.
                    doi: 10.1016/j.molmet.2014.11.004pubmed: 25685688google scholar: lookup
                  64. Uhl PB, Amann B, Hauck SM, Deeg CA. Novel localization of peripherin 2, the photoreceptor-specific retinal degeneration slow protein, in retinal pigment epithelium. Int J Mol Sci 2015 Jan 26;16(2):2678-92.
                    doi: 10.3390/ijms16022678pubmed: 25629227google scholar: lookup
                  65. Vanzo E, Ghirardo A, Merl-Pham J, Lindermayr C, Heller W, Hauck SM, Durner J, Schnitzler JP. S-nitroso-proteome in poplar leaves in response to acute ozone stress. PLoS One 2014;9(9):e106886.
                    doi: 10.1371/journal.pone.0106886pubmed: 25192423google scholar: lookup
                  66. Li X, Gu X, Boyce TM, Zheng M, Reagan AM, Qi H, Mandal N, Cohen AW, Callegan MC, Carr DJ, Elliott MH. Caveolin-1 increases proinflammatory chemoattractants and blood-retinal barrier breakdown but decreases leukocyte recruitment in inflammation. Invest Ophthalmol Vis Sci 2014 Aug 26;55(10):6224-34.
                    doi: 10.1167/iovs.14-14613pubmed: 25159208google scholar: lookup
                  67. Zhao M, Li M, Li X, Shao C, Yin J, Gao Y. Dynamic changes of urinary proteins in a focal segmental glomerulosclerosis rat model. Proteome Sci 2014;12:42.
                    doi: 10.1186/1477-5956-12-42pubmed: 25061428google scholar: lookup
                  68. von Toerne C, Menzler J, Ly A, Senninger N, Ueffing M, Hauck SM. Identification of a novel neurotrophic factor from primary retinal Müller cells using stable isotope labeling by amino acids in cell culture (SILAC). Mol Cell Proteomics 2014 Sep;13(9):2371-81.
                    doi: 10.1074/mcp.M113.033613pubmed: 24925906google scholar: lookup
                  69. Heim A, Grimm C, Müller U, Häußler S, Mackeen MM, Merl J, Hauck SM, Kessler BM, Schofield CJ, Wolf A, Böttger A. Jumonji domain containing protein 6 (Jmjd6) modulates splicing and specifically interacts with arginine-serine-rich (RS) domains of SR- and SR-like proteins. Nucleic Acids Res 2014 Jul;42(12):7833-50.
                    doi: 10.1093/nar/gku488pubmed: 24914048google scholar: lookup
                  70. Klar R, Schober S, Rami M, Mall S, Merl J, Hauck SM, Ueffing M, Admon A, Slotta-Huspenina J, Schwaiger M, Stevanović S, Oostendorp RA, Busch DH, Peschel C, Krackhardt AM. Therapeutic targeting of naturally presented myeloperoxidase-derived HLA peptide ligands on myeloid leukemia cells by TCR-transgenic T cells. Leukemia 2014 Dec;28(12):2355-66.
                    doi: 10.1038/leu.2014.131pubmed: 24736212google scholar: lookup
                  71. Chen YF, Zhou D, Metzger T, Gallup M, Jeanne M, Gould DB, Anderson MS, McNamara NA. Spontaneous development of autoimmune uveitis Is CCR2 dependent. Am J Pathol 2014 Jun;184(6):1695-705.
                    doi: 10.1016/j.ajpath.2014.02.024pubmed: 24736166google scholar: lookup
                  72. Gu X, Reagan A, Yen A, Bhatti F, Cohen AW, Elliott MH. Spatial and temporal localization of caveolin-1 protein in the developing retina. Adv Exp Med Biol 2014;801:15-21.
                    doi: 10.1007/978-1-4614-3209-8_3pubmed: 24664676google scholar: lookup
                  73. Spieler D, Kaffe M, Knauf F, Bessa J, Tena JJ, Giesert F, Schormair B, Tilch E, Lee H, Horsch M, Czamara D, Karbalai N, von Toerne C, Waldenberger M, Gieger C, Lichtner P, Claussnitzer M, Naumann R, Müller-Myhsok B, Torres M, Garrett L, Rozman J, Klingenspor M, Gailus-Durner V, Fuchs H, Hrabě de Angelis M, Beckers J, Hölter SM, Meitinger T, Hauck SM, Laumen H, Wurst W, Casares F, Gómez-Skarmeta JL, Winkelmann J. Restless legs syndrome-associated intronic common variant in Meis1 alters enhancer function in the developing telencephalon. Genome Res 2014 Apr;24(4):592-603.
                    doi: 10.1101/gr.166751.113pubmed: 24642863google scholar: lookup
                  74. Degroote RL, Hauck SM, Amann B, Hirmer S, Ueffing M, Deeg CA. Unraveling the equine lymphocyte proteome: differential septin 7 expression associates with immune cells in equine recurrent uveitis. PLoS One 2014;9(3):e91684.
                    doi: 10.1371/journal.pone.0091684pubmed: 24614191google scholar: lookup
                  75. Griciuc A, Roux MJ, Merl J, Giangrande A, Hauck SM, Aron L, Ueffing M. Proteomic survey reveals altered energetic patterns and metabolic failure prior to retinal degeneration. J Neurosci 2014 Feb 19;34(8):2797-812.
                    doi: 10.1523/JNEUROSCI.2982-13.2014pubmed: 24553922google scholar: lookup
                  76. Han D, Moon S, Kim Y, Min H, Kim Y. Characterization of the membrane proteome and N-glycoproteome in BV-2 mouse microglia by liquid chromatography-tandem mass spectrometry. BMC Genomics 2014 Feb 4;15:95.
                    doi: 10.1186/1471-2164-15-95pubmed: 24495382google scholar: lookup
                  77. Pischedda F, Szczurkowska J, Cirnaru MD, Giesert F, Vezzoli E, Ueffing M, Sala C, Francolini M, Hauck SM, Cancedda L, Piccoli G. A cell surface biotinylation assay to reveal membrane-associated neuronal cues: Negr1 regulates dendritic arborization. Mol Cell Proteomics 2014 Mar;13(3):733-48.
                    doi: 10.1074/mcp.M113.031716pubmed: 24382801google scholar: lookup
                  78. Franko A, Baris OR, Bergschneider E, von Toerne C, Hauck SM, Aichler M, Walch AK, Wurst W, Wiesner RJ, Johnston IC, de Angelis MH. Efficient isolation of pure and functional mitochondria from mouse tissues using automated tissue disruption and enrichment with anti-TOM22 magnetic beads. PLoS One 2013;8(12):e82392.
                    doi: 10.1371/journal.pone.0082392pubmed: 24349272google scholar: lookup
                  79. Gu X, Fliesler SJ, Zhao YY, Stallcup WB, Cohen AW, Elliott MH. Loss of caveolin-1 causes blood-retinal barrier breakdown, venous enlargement, and mural cell alteration. Am J Pathol 2014 Feb;184(2):541-55.
                    doi: 10.1016/j.ajpath.2013.10.022pubmed: 24326256google scholar: lookup
                  80. Mahajan VB, Skeie JM. Translational vitreous proteomics. Proteomics Clin Appl 2014 Apr;8(3-4):204-8.
                    doi: 10.1002/prca.201300062pubmed: 24115652google scholar: lookup
                  81. Ly A, Scheerer MF, Zukunft S, Muschet C, Merl J, Adamski J, de Angelis MH, Neschen S, Hauck SM, Ueffing M. Retinal proteome alterations in a mouse model of type 2 diabetes. Diabetologia 2014 Jan;57(1):192-203.
                    doi: 10.1007/s00125-013-3070-2pubmed: 24078137google scholar: lookup
                  82. Priglinger CS, Szober CM, Priglinger SG, Merl J, Euler KN, Kernt M, Gondi G, Behler J, Geerlof A, Kampik A, Ueffing M, Hauck SM. Galectin-3 induces clustering of CD147 and integrin-β1 transmembrane glycoprotein receptors on the RPE cell surface. PLoS One 2013;8(7):e70011.
                    doi: 10.1371/journal.pone.0070011pubmed: 23922889google scholar: lookup
                  83. Bakshi MV, Barjaktarovic Z, Azimzadeh O, Kempf SJ, Merl J, Hauck SM, Eriksson P, Buratovic S, Atkinson MJ, Tapio S. Long-term effects of acute low-dose ionizing radiation on the neonatal mouse heart: a proteomic study. Radiat Environ Biophys 2013 Nov;52(4):451-61.
                    doi: 10.1007/s00411-013-0483-8pubmed: 23880982google scholar: lookup
                  84. Euler KN, Hauck SM, Ueffing M, Deeg CA. Bovine neonatal pancytopenia--comparative proteomic characterization of two BVD vaccines and the producer cell surface proteome (MDBK). BMC Vet Res 2013 Jan 23;9:18.
                    doi: 10.1186/1746-6148-9-18pubmed: 23343349google scholar: lookup
                  85. Ladd JJ, Chao T, Johnson MM, Qiu J, Chin A, Israel R, Pitteri SJ, Mao J, Wu M, Amon LM, McIntosh M, Li C, Prentice R, Disis N, Hanash S. Autoantibody signatures involving glycolysis and splicesome proteins precede a diagnosis of breast cancer among postmenopausal women. Cancer Res 2013 Mar 1;73(5):1502-13.
                    doi: 10.1158/0008-5472.CAN-12-2560pubmed: 23269276google scholar: lookup
                  86. Swadzba ME, Hauck SM, Naim HY, Amann B, Deeg CA. Retinal glycoprotein enrichment by concanavalin a enabled identification of novel membrane autoantigen synaptotagmin-1 in equine recurrent uveitis. PLoS One 2012;7(12):e50929.
                    doi: 10.1371/journal.pone.0050929pubmed: 23236410google scholar: lookup
                  87. Szober CM, Hauck SM, Euler KN, Fröhlich KJ, Alge-Priglinger C, Ueffing M, Deeg CA. Profound re-organization of cell surface proteome in equine retinal pigment epithelial cells in response to in vitro culturing. Int J Mol Sci 2012 Oct 31;13(11):14053-72.
                    doi: 10.3390/ijms131114053pubmed: 23203049google scholar: lookup
                  88. Li X, McClellan ME, Tanito M, Garteiser P, Towner R, Bissig D, Berkowitz BA, Fliesler SJ, Woodruff ML, Fain GL, Birch DG, Khan MS, Ash JD, Elliott MH. Loss of caveolin-1 impairs retinal function due to disturbance of subretinal microenvironment. J Biol Chem 2012 May 11;287(20):16424-34.
                    doi: 10.1074/jbc.M112.353763pubmed: 22451674google scholar: lookup
                  89. Deeg CA, Eberhardt C, Hofmaier F, Amann B, Hauck SM. Osteopontin and fibronectin levels are decreased in vitreous of autoimmune uveitis and retinal expression of both proteins indicates ECM re-modeling. PLoS One 2011;6(12):e27674.
                    doi: 10.1371/journal.pone.0027674pubmed: 22194789google scholar: lookup
                  90. Wu Z, Doondeea JB, Gholami AM, Janning MC, Lemeer S, Kramer K, Eccles SA, Gollin SM, Grenman R, Walch A, Feller SM, Kuster B. Quantitative chemical proteomics reveals new potential drug targets in head and neck cancer. Mol Cell Proteomics 2011 Dec;10(12):M111.011635.
                    doi: 10.1074/mcp.M111.011635pubmed: 21955398google scholar: lookup
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