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Deep-horn Artificial Insemination With Frozen Thawed Semen After re-extension in Autologous Seminal Plasma May Improve Pregnancy Rates in Jennies.
Abstract: In the literature, the very low pregnancy rates after artificial insemination (AI) with frozen semen in donkeys were improved in one study after re-extension of the frozen-thawed semen in autologous seminal plasma. The aims of our study were (1) to describe in vitro post-thaw parameters of donkey jackass semen after re-extension in seminal plasma (SP) or in INRA96 and (2) to compare pregnancy rates in jennies bred with frozen-thawed semen using two different AI protocols. Semen collected from two Amiata donkey stallions, known to be fertile, was frozen in INRA96 supplemented with 2% egg yolk, and 2.2% glycerol. From the same jacks, seminal plasma was filtered, and stored at -20°C. According to the in vitro analysis, the post-thaw re-extension in SP some kinematic parameters decreased more over time. Two different AI protocols were tested: inseminating deeply into the uterine horn (DHAI) with or without thawed semen re-extension in SP. Higher pregnancy rates were obtained with re-extension in SP than when AIs were performed without SP. Further studies are needed to confirm the advantage of re-extension in autologous SP of donkey frozen-thawed semen before AI in jennies.
Copyright © 2022. Published by Elsevier Inc.
Publication Date: 2022-03-10 PubMed ID: 35283216DOI: 10.1016/j.jevs.2022.103932Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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The research article focuses on improving pregnancy rates in female donkeys, or jennies, from artificial insemination with frozen donkey semen that has been re-extended with the original seminal plasma. It also compares two different protocols of artificial insemination.
Objective of the Study
- The research aimed to investigate the effects of re-extending frozen-thawed donkey semen in its original seminal plasma (SP) and compare the pregnancy outcomes from two distinct artificial insemination (AI) protocols.
Methodology
- The semen used in this study was collected from two known fertile Amiata donkey stallions. It was frozen in a solution known as INRA96 that contained 2% egg yolk and 2.2% glycerol.
- The seminal plasma from the stallions was filtered and preserved at -20 degrees Celsius.
- After thawing the frozen semen, the research team re-extended it in one of two ways: either in the original seminal plasma(SP), or in INRA96.
- They compared how these different media affected the post-thaw parameters of the semen, recording any changes in kinematic parameters over time.
In-vitro Analysis and AI Protocols
- The in-vitro findings showed that certain kinematic parameters of the semen declined more after being re-extended in the original seminal plasma.
- The research also tested two artificial insemination procedures: Deep Horn Artificial Insemination (DHAI) with semen that had been re-extended in SP, and DHAI with semen that had not been re-extended in SP.
Findings
- The research found that higher pregnancy rates were achieved when the AI was performed with the semen that had been re-extended in its original seminal plasma.
- These higher pregnancy rates suggest that the use of autologous seminal plasma might improve the success rate of artificial insemination with frozen-thawed donkey semen.
- However, the researchers note that further studies are needed to determine whether re-extension in autologous seminal plasma is truly advantageous before its broad application in artificial insemination for donkeys.
Cite This Article
APA
Fanelli D, Tesi M, Monaco D, Diaz-Jimenez M, Camillo F, Rota A, Panzani D.
(2022).
Deep-horn Artificial Insemination With Frozen Thawed Semen After re-extension in Autologous Seminal Plasma May Improve Pregnancy Rates in Jennies.
J Equine Vet Sci, 112, 103932.
https://doi.org/10.1016/j.jevs.2022.103932 Publication
Researcher Affiliations
- Veterinary Sciences Department, Pisa University, Pisa, Italy. Electronic address: dianafanelli@alice.it.
- Veterinary Sciences Department, Pisa University, Pisa, Italy.
- Department of Veterinary Medicine, University of Bari "Aldo Moro," Bari, Italy.
- Veterinary Reproduction Group, Department of Animal Medicine and Surgery, University of Cordoba, Cordoba, Spain.
- Veterinary Sciences Department, Pisa University, Pisa, Italy.
- Veterinary Sciences Department, Pisa University, Pisa, Italy.
- Veterinary Sciences Department, Pisa University, Pisa, Italy.
MeSH Terms
- Animals
- Cryopreservation / veterinary
- Equidae
- Female
- Horses
- Insemination, Artificial / veterinary
- Male
- Plant Breeding
- Pregnancy
- Pregnancy Rate
- Semen Preservation / veterinary
Citations
This article has been cited 2 times.- Seyedasgari F, Asadi B, Kim E. Seminal plasma modulates post-thaw longevity and motility of frozen sperm in dromedary camel. Anim Biosci 2023 Dec;36(12):1821-1830.
- Fanelli D, Moroni R, Bocci C, Camillo F, Rota A, Panzani D. Interspecific and Intraspecific Artificial Insemination in Domestic Equids. Animals (Basel) 2023 Feb 7;13(4).
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