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Home / Equine Research Bank / Reprod Biol Endocrinol / Delta opioid receptor on equine sperm cells: subcellular loc...
Reproductive biology and endocrinology : RB&E2010; 8; 78; doi: 10.1186/1477-7827-8-78

Delta opioid receptor on equine sperm cells: subcellular localization and involvement in sperm motility analyzed by computer assisted sperm analyzer (CASA).

Abstract: Opioid receptors and endogenous opioid peptides act not only in the control of nociceptive pathways, indeed several reports demonstrate the effects of opiates on sperm cell motility and morphology suggesting the importance of these receptors in the modulation of reproduction in mammals. In this study we investigated the expression of delta opioid receptors on equine spermatozoa by western blot/indirect immunofluorescence and its relationship with sperm cell physiology. Methods: We analyzed viability, motility, capacitation, acrosome reaction and mitochondrial activity in the presence of naltrindole and DPDPE by means of a computer assisted sperm analyzer and a fluorescent confocal microscope. The evaluation of viability, capacitation and acrosome reaction was carried out by the double CTC/Hoechst staining, whereas mitochondrial activity was assessed by means of MitoTracker Orange dye. Results: We showed that in equine sperm cells, delta opioid receptor is expressed as a doublet of 65 and 50 kDa molecular mass and is localized in the mid piece of tail; we also demonstrated that naltrindole, a delta opioid receptor antagonist, could be utilized in modulating several physiological parameters of the equine spermatozoon in a dose-dependent way. We also found that low concentrations of the antagonist increase sperm motility whereas high concentrations show the opposite effect. Moreover low concentrations hamper capacitation, acrosome reaction and viability even if the percentage of cells with active mitochondria seems to be increased; the opposite effect is exerted at high concentrations. We have also observed that the delta opioid receptor agonist DPDPE is scarcely involved in affecting the same parameters at the employed concentrations. Conclusions: The results described in this paper add new important details in the comprehension of the mammalian sperm physiology and suggest new insights for improving reproduction and for optimizing equine breeding.
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Publication Date: 2010-06-25 PubMed ID: 20579355PubMed Central: PMC2901311DOI: 10.1186/1477-7827-8-78Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research focuses on understanding the role and impact of delta opioid receptors on the physiology of equine sperm cells. Researchers observed that the receptor can be used to manipulate several physiological parameters of equine sperm, potentially advancing the understanding of mammalian sperm physiology and aiding in optimizing equine breeding.

Objectives of the Study

  • The study aimed to investigate the expression of delta opioid receptors on equine sperm cells.
  • Its focus was to understand the relationship of this receptor with sperm cell physiology, including viability, motility, capacitation, acrosome reaction, and mitochondrial activity.

Methods Used in the Study

  • Researchers used western blot/indirect immunofluorescence to identify the expression of delta opioid receptors on the sperm cells.
  • The physiological aspects of sperm cells were investigated in the presence of naltrindole (a delta opioid receptor antagonist) and DPDPE (a delta opioid receptor agonist), using computer assisted sperm analyzer and a fluorescent confocal microscope.
  • The evaluation of viability, capacitation, and acrosome reaction were carried out through double CTC/Hoechst staining, and mitochondrial activity was assessed with MitoTracker Orange dye.

Key Findings

  • The delta opioid receptor is present as a doublet of 65 and 50 kDa molecular mass on equine sperm cells, primarily localized in the mid piece of tail.
  • Naltrindole was found to alter several physiological parameters of the equine spermatozoa in a dose-dependent manner. Lower concentrations increased sperm motility, while higher concentrations proved detrimental. Lower doses also hampered capacitation, acrosome reaction, and viability, but appeared to increase the percentage of cells with active mitochondria. Conversely, higher doses had the opposite effect.
  • DPDPE, the delta opioid receptor agonist, showed negligible effect on these parameters at the used concentrations.

Conclusions and Implications

  • The findings provide crucial details about the role of delta opioid receptors in regulating the physiology of mammalian sperm cells.
  • This knowledge could potentially serve in improving mammalian reproduction methodologies and optimize equine breeding.

Cite This Article

APA
Albrizio M, Lacalandra GM, Micera E, Guaricci AC, Nicassio M, Zarrilli A. (2010). Delta opioid receptor on equine sperm cells: subcellular localization and involvement in sperm motility analyzed by computer assisted sperm analyzer (CASA). Reprod Biol Endocrinol, 8, 78. https://doi.org/10.1186/1477-7827-8-78

Publication

Reproductive biology and endocrinology : RB&E
ISSN: 1477-7827
NlmUniqueID: 101153627
Country: England
Language: English
Volume: 8
Pages: 78

Researcher Affiliations

Albrizio, Maria
  • Department of Animal Production, Faculty of Veterinary Medicine, University of Bari, I-70010, Valenzano (BA), Italy. m.albrizio@veterinaria.uniba.it
Lacalandra, Giovanni M
    Micera, Elisabetta
      Guaricci, Antonio C
        Nicassio, Michele
          Zarrilli, Antonia

            MeSH Terms

            • Animals
            • Cell Survival / drug effects
            • Enkephalin, D-Penicillamine (2,5)- / pharmacology
            • Horses / metabolism
            • Image Processing, Computer-Assisted / methods
            • Male
            • Naltrexone / analogs & derivatives
            • Naltrexone / pharmacology
            • Receptors, Opioid, delta / agonists
            • Receptors, Opioid, delta / antagonists & inhibitors
            • Receptors, Opioid, delta / metabolism
            • Semen Analysis / instrumentation
            • Semen Analysis / methods
            • Sperm Motility / drug effects
            • Sperm Motility / physiology
            • Spermatozoa / drug effects
            • Spermatozoa / metabolism
            • Subcellular Fractions / metabolism
            • Tissue Distribution / drug effects

            References

            This article includes 44 references
            1. Elde R, Hökfelt T. Coexistence of opioid peptides with other neurotransmitters. Handbook of experimental pharmacology, Opioids Vol. 1. Berlin: Springer; 1993; pp. 585–624.
            2. Bostwick DG, Null WE, Holmes D, Weber E, Barchas JD, Bensch KG. Expression of opioid peptides in tumors.. N Engl J Med 1987 Dec 3;317(23):1439-43.
              pubmed: 2891033doi: 10.1056/nejm198712033172304google scholar: lookup
            3. Fabbri A, Jannini EA, Gnessi L, Ulisse S, Moretti C, Isidori A. Neuroendocrine control of male reproductive function. The opioid system as a model of control at multiple sites.. J Steroid Biochem 1989 Jan;32(1B):145-50.
              pubmed: 2643737doi: 10.1016/0022-4731(89)90155-6google scholar: lookup
            4. Foresta C, Caretto A, Indino M, Betterle C, Scandellari C. Localization of met-enkephalin on human spermatozoa and evidence for its physiological role.. Arch Androl 1986;17(1):19-24.
              doi: 10.3109/01485018608986952pubmed: 3539053google scholar: lookup
            5. Cupo A, Menezo Y, Bueno L. Enkephalin production by the corpus luteum.. Neuropeptides 1987 Apr;9(3):237-45.
              doi: 10.1016/0143-4179(87)90044-8pubmed: 3601012google scholar: lookup
            6. Tam PP, Ng TB, Mao KR. Beta-endorphin levels in the preovulatory follicles and the outcome of in vitro fertilization.. J In Vitro Fert Embryo Transf 1988 Apr;5(2):91-5.
              doi: 10.1007/BF01130665pubmed: 2970512google scholar: lookup
            7. Kew D, Muffly KE, Kilpatrick DL. Proenkephalin products are stored in the sperm acrosome and may function in fertilization.. Proc Natl Acad Sci U S A 1990 Dec;87(23):9143-7.
              doi: 10.1073/pnas.87.23.9143pmc: PMC55120pubmed: 1701253google scholar: lookup
            8. Staszkiewicz J, Skowronski MT, Kaminski T, Siawrys G, Krazinski BE, Kusmider M, Przala J, Okrasa S. Expression of proopiomelanocortin, proenkephalin and prodynorphin genes in porcine theca and granulosa cells.. Anim Reprod Sci 2007 Sep;101(1-2):97-112.
              doi: 10.1016/j.anireprosci.2006.09.003pubmed: 17023126google scholar: lookup
            9. Pasternak GW. Multiple opiate receptors: déjà vu all over again.. Neuropharmacology 2004;47 Suppl 1:312-23.
              doi: 10.1016/j.neuropharm.2004.07.004pubmed: 15464147google scholar: lookup
            10. Albrizio M, Guaricci AC, Maritato F, Sciorsci RL, Mari G, Calamita G, Lacalandra GM, Aiudi GG, Minoia R, Dell'Aquila ME, Minoia P. Expression and subcellular localization of the mu-opioid receptor in equine spermatozoa: evidence for its functional role.. Reproduction 2005 Jan;129(1):39-49.
              doi: 10.1530/rep.1.00284pubmed: 15615897google scholar: lookup
            11. Albrizio M, Guaricci AC, Calamita G, Zarrilli A, Minoia P. Expression and immunolocalization of the mu-opioid receptor in human sperm cells.. Fertil Steril 2006 Dec;86(6):1776-9.
              doi: 10.1016/j.fertnstert.2006.04.037pubmed: 17007854google scholar: lookup
            12. Agirregoitia E, Valdivia A, Carracedo A, Casis L, Gil J, Subiran N, Ochoa C, Irazusta J. Expression and localization of delta-, kappa-, and mu-opioid receptors in human spermatozoa and implications for sperm motility.. J Clin Endocrinol Metab 2006 Dec;91(12):4969-75.
              doi: 10.1210/jc.2006-0599pubmed: 16984994google scholar: lookup
            13. Massotte D, Kieffer BL. A molecular basis for opioid action. Essays in Biochemistry: molecular biology of the brain London: Portland Press; 1998; pp. 65–77.
            14. Cariello CL, Zanet L, Spagnuolo A, Nelson L. Effects of opioids and antagonists on the rate of sea urchin sperm progressive motility. Biol Bull 1986;171:208–216.
              doi: 10.2307/1541918google scholar: lookup
            15. Ragni G, De Lauretis L, Bestetti O, Sghedoni D, Gambaro V. Gonadal function in male heroin and methadone addicts.. Int J Androl 1988 Apr;11(2):93-100.
              doi: 10.1111/j.1365-2605.1988.tb00984.xpubmed: 3372047google scholar: lookup
            16. Cacciola G, Chioccarelli T, Ricci G, Meccariello R, Fasano S, Pierantoni R, Cobellis G. The endocannabinoid system in vertebrate male reproduction: a comparative overview.. Mol Cell Endocrinol 2008 Apr 16;286(1-2 Suppl 1):S24-30.
              doi: 10.1016/j.mce.2008.01.004pubmed: 18342433google scholar: lookup
            17. Singer R, Ben-Bassat M, Malik Z, Sagiv M, Ravid A, Shohat B, Livni E, Mamon T, Segenreich E, Servadio C. Oligozoospermia, asthenozoospermia, and sperm abnormalities in ex-addict to heroin, morphine, and hashish.. Arch Androl 1986;16(2):167-74.
              doi: 10.3109/01485018608986938pubmed: 3488721google scholar: lookup
            18. Childers SR. Opioid receptor-coupled second messenger systems.. Life Sci 1991;48(21):1991-2003.
              doi: 10.1016/0024-3205(91)90154-4pubmed: 1851914google scholar: lookup
            19. Mcnally GP, Akil H. Opioid peptides and their receptors: overview and function in pain modulation. Neuropsychopharmacology: the fifth generation of progress New York: Lippincott, Williams & Wilkins; 2002; pp. 35–46.
            20. Bean BP. Neurotransmitter inhibition of neuronal calcium currents by changes in channel voltage dependence.. Nature 1989 Jul 13;340(6229):153-6.
              doi: 10.1038/340153a0pubmed: 2567963google scholar: lookup
            21. North RA. Opioid actions on membrane ion channels. Opioids 1 Berlin: Springer-Verlag; 1993; pp. 773–797.
            22. Whistler JL, Chuang HH, Chu P, Jan LY, von Zastrow M. Functional dissociation of mu opioid receptor signaling and endocytosis: implications for the biology of opiate tolerance and addiction.. Neuron 1999 Aug;23(4):737-46.
              doi: 10.1016/S0896-6273(01)80032-5pubmed: 10482240google scholar: lookup
            23. Kim KW, Kim SJ, Shin BS, Choi HY. Ligand binding profiles of delta-opioid receptor in human cerebral cortex membranes: evidence of delta-opioid receptor heterogeneity.. Life Sci 2001 Feb 23;68(14):1649-56.
              doi: 10.1016/S0024-3205(01)00962-6pubmed: 11263677google scholar: lookup
            24. Zaki PA, Bilsky EJ, Vanderah TW, Lai J, Evans CJ, Porreca F. Opioid receptor types and subtypes: the delta receptor as a model.. Annu Rev Pharmacol Toxicol 1996;36:379-401.
              doi: 10.1146/annurev.pa.36.040196.002115pubmed: 8725395google scholar: lookup
            25. Zukin RS, Eghbali M, Olive D, Unterwald EM, Tempel A. Characterization and visualization of rat and guinea pig brain kappa opioid receptors: evidence for kappa 1 and kappa 2 opioid receptors.. Proc Natl Acad Sci U S A 1988 Jun;85(11):4061-5.
              doi: 10.1073/pnas.85.11.4061pmc: PMC280361pubmed: 2836869google scholar: lookup
            26. George SR, Fan T, Xie Z, Tse R, Tam V, Varghese G, O'Dowd BF. Oligomerization of mu- and delta-opioid receptors. Generation of novel functional properties.. J Biol Chem 2000 Aug 25;275(34):26128-35.
              doi: 10.1074/jbc.M000345200pubmed: 10842167google scholar: lookup
            27. Belcheva MM, Ignatova EG, Young EC, Coscia CJ. Agonist-induced desensitization and down-regulation of delta opioid receptors alter the levels of their 125I-beta-endorphin cross-linked products in subcellular fractions from NG108-15 cells.. Biochemistry 1996 Nov 26;35(47):14818-24.
              doi: 10.1021/bi961579+pubmed: 8942644google scholar: lookup
            28. Miller BC. Western blot analysis of the delta (delta)-opioid receptor in activated murine T cells.. Adv Exp Med Biol 1998;437:159-67.
              pubmed: 9666267doi: 10.1007/978-1-4615-5347-2_17google scholar: lookup
            29. Persson AI, Thorlin T, Eriksson PS. Comparison of immunoblotted delta opioid receptor proteins expressed in the adult rat brain and their regulation by growth hormone.. Neurosci Res 2005 May;52(1):1-9.
              doi: 10.1016/j.neures.2005.01.003pubmed: 15811547google scholar: lookup
            30. Eguchi M. Recent advances in selective opioid receptor agonists and antagonists.. Med Res Rev 2004 Mar;24(2):182-212.
              doi: 10.1002/med.10059pubmed: 14705168google scholar: lookup
            31. Chang KJ, Cooper BR, Hazum E, Cuatrecasas P. Multiple opiate receptors: different regional distribution in the brain and differential binding of opiates and opioid peptides.. Mol Pharmacol 1979 Jul;16(1):91-104.
              pubmed: 225656
            32. Davidson A, Vermesh M, Paulson RJ, Graczykowski JW, Lobo RA. Presence of immunoreactive beta-endorphin and calcitonin in human seminal plasma, and their relation to sperm physiology.. Fertil Steril 1989 May;51(5):878-80.
              pubmed: 2523323doi: 10.1016/s0015-0282(16)60684-2google scholar: lookup
            33. Balboni G, Salvadori S, Dal Piaz A, Bortolotti F, Argazzi R, Negri L, Lattanzi R, Bryant SD, Jinsmaa Y, Lazarus LH. Highly selective fluorescent analogue of the potent delta-opioid receptor antagonist Dmt-Tic.. J Med Chem 2004 Dec 16;47(26):6541-6.
              doi: 10.1021/jm040128hpubmed: 15588089google scholar: lookup
            34. Garner DL, Thomas CA, Joerg HW, DeJarnette JM, Marshall CE. Fluorometric assessments of mitochondrial function and viability in cryopreserved bovine spermatozoa.. Biol Reprod 1997 Dec;57(6):1401-6.
              doi: 10.1095/biolreprod57.6.1401pubmed: 9408246google scholar: lookup
            35. Gravance CG, Garner DL, Miller MG, Berger T. Fluorescent probes and flow cytometry to assess rat sperm integrity and mitochondrial function.. Reprod Toxicol 2001 Jan-Feb;15(1):5-10.
              doi: 10.1016/S0890-6238(00)00113-1pubmed: 11137373google scholar: lookup
            36. Howard AD, de La Baume S, Gioannini TL, Hiller JM, Simon EJ. Covalent labeling of opioid receptors with radioiodinated human beta-endorphin. Identification of binding site subunit.. J Biol Chem 1985 Sep 5;260(19):10833-9.
              pubmed: 2993292
            37. Anand DJ, Oommen A. A molecular weight study of the rat brain delta opioid receptor.. Indian J Biochem Biophys 1995 Jun;32(3):161-5.
              pubmed: 7590858
            38. Christoffers KH, Li H, Howells RD. Purification and mass spectrometric analysis of the delta opioid receptor.. Brain Res Mol Brain Res 2005 May 20;136(1-2):54-64.
              doi: 10.1016/j.molbrainres.2005.01.016pubmed: 15893587google scholar: lookup
            39. Albrizio M, Micera E, De Santis T, Dell'Aquila ME, Zarrilli A. Subcellular localization of delta opioid receptors and L-type voltage channels on equine sperm cells by indirect immunofluorescence. Reprod Domest Anim 2007;42(Suppl 2):85.
            40. Feigenbaum JJ, Howard SG. Effects of naloxone on amphetamine induced striatal dopamine release in vivo: a microdialysis study.. Life Sci 1997;60(19):1659-68.
              doi: 10.1016/S0024-3205(97)00108-2pubmed: 9129121google scholar: lookup
            41. Fukuda K, Kato S, Shoda T, Morikawa H, Mima H, Mori K. Partial agonistic activity of naloxone on the opioid receptors expressed from complementary deoxyribonucleic acids in Chinese hamster ovary cells.. Anesth Analg 1998 Aug;87(2):450-5.
              doi: 10.1097/00000539-199808000-00041pubmed: 9706949google scholar: lookup
            42. Di Sole F, Guerra L, Bagorda A, Reshkin SJ, Albrizio M, Minoia P, Casavola V. Naloxone inhibits A6 cell Na(+)/H(+) exchange by activating protein kinase C via the mobilization of intracellular calcium.. Exp Nephrol 2001;9(5):341-8.
              doi: 10.1159/000052630pubmed: 11549852google scholar: lookup
            43. Dell'Aquila ME, Casavola V, Reshkin SJ, Albrizio M, Guerra L, Maritato F, Minoia P. Effects of beta-endorphin and Naloxone on in vitro maturation of bovine oocytes.. Mol Reprod Dev 2002 Oct;63(2):210-22.
              doi: 10.1002/mrd.10163pubmed: 12203831google scholar: lookup
            44. Dell'Aquila ME, Albrizio M, Guaricci AC, De Santis T, Maritato F, Tremoleda JL, Colenbrander B, Guerra L, Casavola V, Minoia P. Expression and localization of the mu-opioid receptor (MOR) in the equine cumulus-oocyte complex and its involvement in the seasonal regulation of oocyte meiotic competence.. Mol Reprod Dev 2008 Aug;75(8):1229-46.
              doi: 10.1002/mrd.20869pubmed: 18213645google scholar: lookup

            Citations

            This article has been cited 5 times.
            1. Ghasemi-Esmailabad S, Talebi AH, Talebi AR, Amiri S, Moshrefi M, Pourentezari M. The effects of morphine abuse on sperm parameters, chromatin integrity and apoptosis in men. JBRA Assist Reprod 2022 Aug 4;26(3):444-449.
              doi: 10.5935/1518-0557.20210110pubmed: 34995046google scholar: lookup
            2. Albrizio M, Guaricci AC, Milano S, Macrì F, Aiudi G. Mu opioid receptor in spermatozoa, eggs and larvae of gilthead sea bream (Sparus Aurata) and its involvement in stress related to aquaculture. Fish Physiol Biochem 2014 Aug;40(4):997-1009.
              doi: 10.1007/s10695-013-9900-9pubmed: 24338156google scholar: lookup
            3. Subirán N, Casis L, Irazusta J. Regulation of male fertility by the opioid system. Mol Med 2011;17(7-8):846-53.
              doi: 10.2119/molmed.2010.00268pubmed: 21431247google scholar: lookup
            4. Vicente-Carrillo A, Álvarez-Rodríguez M, Castaño C, Toledano-Díaz A, Martínez-Nevado E, Rodríguez-Martínez H, Santiago-Moreno J. Presence and Location of CatSper 1-4, Opioid (μ, δ and κ) and CD44 Receptors in SPERMATOZOA from AOUDAD, IBERIAN IBEX and Mouflon. Vet Med Sci 2025 Jul;11(4):e70459.
              doi: 10.1002/vms3.70459pubmed: 40679138google scholar: lookup
            5. Vassoler FM, Wimmer ME. Consequences of Parental Opioid Exposure on Neurophysiology, Behavior, and Health in the Next Generations. Cold Spring Harb Perspect Med 2021 Oct 1;11(10).
              doi: 10.1101/cshperspect.a040436pubmed: 32601130google scholar: lookup
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