Demographics of natural oral infection of mosquitos by Venezuelan equine encephalitis virus.
Abstract: The within-host diversity of virus populations can be drastically limited during between-host transmission, with primary infection of hosts representing a major constraint to diversity maintenance. However, there is an extreme paucity of quantitative data on the demographic changes experienced by virus populations during primary infection. Here, the multiplicity of cellular infection (MOI) and population bottlenecks were quantified during primary mosquito infection by Venezuelan equine encephalitis virus, an arbovirus causing neurological disease in humans and equids.
Copyright © 2015, American Society for Microbiology. All Rights Reserved.
Publication Date: 2015-01-14 PubMed ID: 25589654PubMed Central: PMC4403435DOI: 10.1128/JVI.03265-14Google Scholar: Lookup
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- Journal Article
- Research Support
- N.I.H.
- Extramural
- Animal Models
- Animal Science
- Arboviruses
- Disease control
- Disease Prevalence
- Disease Transmission
- Epidemiology
- Equine Health
- Infection
- Infectious Disease
- Mosquito-borne Diseases
- Neurological Diseases
- Pathogens
- Population Dynamics
- Public Health
- Vector-borne disease
- Venezuelan Equine Encephalitis
- Veterinary Medicine
- Virus
- Zoonotic Diseases
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This study investigates the changes in population and infection degree of the Venezuelan equine encephalitis virus (VEEV) during its primary infection in mosquitoes. The insights unearthed could be significant in understanding how diseases spread and evolve.
Objective and Importance of the Study
- The primary objective of this research was to delve into the demographic changes that virus populations undergo during primary infection, a topic that has, until now, been inadequately explored.
- The virus in focus was the Venezuelan equine encephalitis virus (VEEV), an arbovirus that leads to neurological diseases in humans and equids. This virus utilizes mosquitoes as its transmission vector, spreading from host to host through mosquito bites.
- The findings from such studies are crucial in comprehending how viruses evolve and spread, and could also help devise methods to curb outbreaks.
Methodology and Concepts Explored
- The researchers quantified two parameters during the primary mosquito infection by VEEV: the multiplicity of cellular infection (MOI) and population bottlenecks.
- The MOI refers to the average number of virus particles that infect a cell. This measurement can shed light on how aggressively the virus multiplies within host cells.
- A population bottleneck refers to a sharp reduction in a population’s size due to environmental events or human activities. In this context, it represents the drastic limitation on virus diversity during transmission between hosts.
Implications of the Research
- The study gives us quantitative insights on the challenges a virus population faces during primary infection in the host. These challenges could potentially impact the success of the infection and further replication of the virus.
- Understanding the intricacies of bottlenecks and MOI could lead to development of strategies to limit viral spread and maintain control during outbreaks.
Cite This Article
APA
Gutiérrez S, Thébaud G, Smith DR, Kenney JL, Weaver SC.
(2015).
Demographics of natural oral infection of mosquitos by Venezuelan equine encephalitis virus.
J Virol, 89(7), 4020-4022.
https://doi.org/10.1128/JVI.03265-14 Publication
Researcher Affiliations
- Cirad, UMR15 CMAEE, Montpellier, France INRA, UMR1309 CMAEE, Montpellier, France serafin.gutierrez@cirad.fr sweaver@utmb.edu.
- INRA, UMR 385 BGPI, Montpellier, France.
- Institute for Human Infections and Immunity, Center for Tropical Diseases, and Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA.
- Institute for Human Infections and Immunity, Center for Tropical Diseases, and Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA.
- Institute for Human Infections and Immunity, Center for Tropical Diseases, and Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA serafin.gutierrez@cirad.fr sweaver@utmb.edu.
MeSH Terms
- Animals
- Culicidae / virology
- Encephalitis Virus, Venezuelan Equine / classification
- Encephalitis Virus, Venezuelan Equine / genetics
- Encephalitis Virus, Venezuelan Equine / isolation & purification
- Genetic Variation
- Insect Vectors
- Mouth / virology
Grant Funding
- R01 AI048807 / NIAID NIH HHS
- AI48807 / NIAID NIH HHS
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Citations
This article has been cited 8 times.- Pontremoli C, Forni D, Clerici M, Cagliani R, Sironi M. Alternation between taxonomically divergent hosts is not the major determinant of flavivirus evolution.. Virus Evol 2021 Jan;7(1):veab040.
- Warmbrod KL, Patterson EI, Kautz TF, Stanton A, Rockx-Brouwer D, Kalveram BK, Khanipov K, Thangamani S, Fofanov Y, Forrester NL. Viral RNA-dependent RNA polymerase mutants display an altered mutation spectrum resulting in attenuation in both mosquito and vertebrate hosts.. PLoS Pathog 2019 Apr;15(4):e1007610.
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