Demonstration of Borna disease virus RNA in peripheral blood mononuclear cells from healthy horses in Japan.
Abstract: Borna disease (BD) is a progressive poliomeningoencephalomyelitis which occurs naturally in horses and sheep. Here, peripheral blood mononuclear cells (PBMC) derived from 57 healthy horses in Japan were examined by a nested reverse transcription-polymerase chain reaction to determine the prevalence of BD virus (BDV) infection. Seventeen (29.8%) of the samples were positive by this examination and the specificity of the amplified product was confirmed by hybridization with authentic oligomer probes. About 60% of the BDV RNA-positive individuals also showed seropositivity by Western blotting. This report is the first for the demonstration of BDV RNA in PBMC of healthy horses, as well as the first on the BDV infection in horses in Japan. Thus, BDV may be more widespread in healthy horses over the world as well as in Japan and the detection of BDV RNA in PBMC at a high rate indicates that the disease might develop in a part of the carriers only after long-incubation period.
Publication Date: 1995-08-01 PubMed ID: 7491814DOI: 10.1016/0264-410x(95)00050-bGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research identifies the presence of Borna disease virus RNA in the blood cells of healthy horses in Japan, concluding that the disease may be more spread and could develop in carriers after a long period of incubation.
Objective of the Research
- The research aimed to determine the prevalence of Borna disease virus (BDV) infection in healthy horses in Japan.
Methodology
- The research examined peripheral blood mononuclear cells (PBMC) from 57 healthy horses through a nested reverse transcription-polymerase chain reaction.
- The specificity of the amplified product was confirmed using authentic oligomer probes.
- BDV RNA-positive individuals were further screened by Western blotting to determine seropositivity.
Key Findings
- BDV RNA was found in 17 of the 57 samples, representing 29.8% of the tested population.
- About 60% of these BDV RNA-positive individuals also showed seropositivity through Western blotting.
Significance of the Study
- This study is the first to demonstrate the presence of BDV RNA in the PBMC of healthy horses and to record BDV infection in horses in Japan.
- The findings suggest a potentially wider prevalence of BDV in healthy horse populations worldwide, not just in Japan.
- The presence of BDV RNA at a high rate could indicate that the disease might develop in a certain portion of carriers after a long-incubation period, hence the need for continuous monitoring.
Cite This Article
APA
Nakamura Y, Kishi M, Nakaya T, Asahi S, Tanaka H, Sentsui H, Ikeda K, Ikuta K.
(1995).
Demonstration of Borna disease virus RNA in peripheral blood mononuclear cells from healthy horses in Japan.
Vaccine, 13(12), 1076-1079.
https://doi.org/10.1016/0264-410x(95)00050-b Publication
Researcher Affiliations
- Section of Serology, Hokkaido University, Sapporo, Japan.
MeSH Terms
- Animals
- Base Sequence
- Borna disease virus / genetics
- Female
- Horses / virology
- Leukocytes, Mononuclear / virology
- Male
- Molecular Sequence Data
- Polymerase Chain Reaction
- RNA, Viral / blood
Citations
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- Zhang L, Lei Y, Liu X, Wang X, Liu Z, Li D, Zheng P, Zhang L, Chen S, Xie P. Glutamate and lipid metabolic perturbation in the hippocampi of asymptomatic borna disease virus-infected horses.. PLoS One 2014;9(6):e99752.
- Song JW, Na KS, Tae SH, Kim YK. Borna disease virus antibody and RNA from peripheral blood mononuclear cells of race horses and jockeys in Korea.. Psychiatry Investig 2011 Mar;8(1):58-60.
- Hagiwara K, Tsuge Y, Asakawa M, Kabaya H, Okamoto M, Miyasho T, Taniyama H, Ishihara C, de la Torre JC, Ikuta K. Borna disease virus RNA detected in Japanese macaques (Macaca fuscata).. Primates 2008 Jan;49(1):57-64.
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- Gonzalez-Dunia D, Sauder C, de la Torre JC. Borna disease virus and the brain.. Brain Res Bull 1997;44(6):647-64.
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- Hagiwara K, Kawamoto S, Takahashi H, Nakamura Y, Nakaya T, Hiramune T, Ishihara C, Ikuta K. High prevalence of Borna disease virus infection in healthy sheep in Japan.. Clin Diagn Lab Immunol 1997 May;4(3):339-44.
- Sauder C, Müller A, Cubitt B, Mayer J, Steinmetz J, Trabert W, Ziegler B, Wanke K, Mueller-Lantzsch N, de la Torre JC, Grässer FA. Detection of Borna disease virus (BDV) antibodies and BDV RNA in psychiatric patients: evidence for high sequence conservation of human blood-derived BDV RNA.. J Virol 1996 Nov;70(11):7713-24.
- Auwanit W, Ayuthaya PI, Nakaya T, Fujiwara S, Kurata T, Yamanishi K, Ikuta K. Unusually high seroprevalence of Borna disease virus in clade E human immunodeficiency virus type 1-infected patients with sexually transmitted diseases in Thailand.. Clin Diagn Lab Immunol 1996 Sep;3(5):590-3.
- Nakamura Y, Asahi S, Nakaya T, Bahmani MK, Saitoh S, Yasui K, Mayama H, Hagiwara K, Ishihara C, Ikuta K. Demonstration of borna disease virus RNA in peripheral blood mononuclear cells derived from domestic cats in Japan.. J Clin Microbiol 1996 Jan;34(1):188-91.
- Kishi M, Nakaya T, Nakamura Y, Kakinuma M, Takahashi TA, Sekiguchi S, Uchikawa M, Tadokoro K, Ikeda K, Ikuta K. Prevalence of Borna disease virus RNA in peripheral blood mononuclear cells from blood donors.. Med Microbiol Immunol 1995 Oct;184(3):135-8.
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