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Home / Equine Research Bank / Vector Borne Zoonotic Dis / Demonstration of Usutu virus antibodies in horses, Croatia.
Vector borne and zoonotic diseases (Larchmont, N.Y.)2013; 13(10); 772-774; doi: 10.1089/vbz.2012.1236

Demonstration of Usutu virus antibodies in horses, Croatia.

Abstract: We report the first serological evidence of Usutu virus (USUV) infection in horses in Croatia. During 2011, 1380 horse serum samples from healthy animals were collected from six northern Croatian counties. All samples were first screened for West Nile virus (WNV) immunoglobulin G (IgG) antibodies using an enzyme-linked immunosorbent assay (ELISA). Sixty-nine WNV ELISA-reactive samples were further tested for WNV antibodies by a virus neutralization assay (VN assay) and plaque reduction neutralization test (PRNT), and USUV by a VN assay and tick-borne encephalitis virus (TBEV) antibodies by PRNT. During the same period, 306 human serum samples from patients coming for routine testing with no symptoms of acute febrile disease were tested for USUV IgG using ELISA. Reactive samples were tested for both USUV and WNV using a VN assay. USUV-specific neutralizing antibodies were detected in two of 69 WNV ELISA-reactive horse serum samples. Seropositive animals were found in two different regions of Croatia. One additional sample showed specific WNV-neutralizing antibodies that cross-neutralized USUV. Only one human sample (0.3%) was reactive to USUV antibodies in an ELISA test. In a confirmatory test, WNV-neutralizing antibodies were detected, indicating cross-reactive antibodies with USUV in ELISA. The exposure to USUV was documented in two WNV ELISA-reactive horses at distant locations. These results indicate the presence of USUV in northern Croatia.
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Publication Date: 2013-06-29 PubMed ID: 23808977DOI: 10.1089/vbz.2012.1236Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research demonstrates the first documented presence of Usutu virus (an infection typically carried by mosquitoes) in horses in Croatia, revealed through serological (blood) tests during the year of 2011. The researchers also examined the presence of this virus in a smaller selection of tested humans.

Background and Methodology

  • The research was conducted in the wake of an increase in West Nile virus (WNV) and Usutu virus (USUV) occurrences in Europe.
  • The researchers collected 1380 horse serum samples from healthy horses across six counties in Northern Croatia over the course of 2011.
  • In order to identify the potential presence of the USUV, they initially screened all collected samples for WNV immunoglobulin G (IgG) antibodies using an enzyme-linked immunosorbent assay (ELISA).
  • A smaller selection of samples (69 in total) that showed a response to WNV in the ELISA test was further examined for WNV and USUV with additional tests, including a virus neutralization assay (VN assay), a plaque reduction neutralization test (PRNT) and a tick-borne encephalitis virus (TBEV) test.

Investigation on Human Samples

  • Alongside horses, 306 human serum samples were also collected and examined in the same year.
  • These humans were chosen as they were partaking in routine medical tests and did not show symptoms of acute febrile disease, a condition typically associated with mosquito-borne infections such as USUV.
  • These human samples were also subjected to ELISA tests for antibodies against USUV. If the ELISA test was positive for USUV, further tests were conducted to validate the results.

Results and Findings

  • The research found that two of the 69 horse serum samples which initially showed a response to the WNV ELISA test had USUV-specific neutralizing antibodies, indicating the presence of USUV in these horses.
  • This marked the first time USUV was identified in horses from Croatia, and these two horses were from different regions of Croatia, suggesting the spread of the virus.
  • One additional horse serum sample was found to contain WNV-neutralizing antibodies that could also neutralize USUV.
  • In human samples however, only one (0.3%) reacted to USUV antibodies in an ELISA test.
  • The serological test results in both horses and humans thus highlight the presence of USUV in Northern Croatia, affirming the need for further epidemiological studies in this region.

Cite This Article

APA
Barbic L, Vilibic-Cavlek T, Listes E, Stevanovic V, Gjenero-Margan I, Ljubin-Sternak S, Pem-Novosel I, Listes I, Mlinaric-Galinovic G, Di Gennaro A, Savini G. (2013). Demonstration of Usutu virus antibodies in horses, Croatia. Vector Borne Zoonotic Dis, 13(10), 772-774. https://doi.org/10.1089/vbz.2012.1236

Publication

Vector borne and zoonotic diseases (Larchmont, N.Y.)
ISSN: 1557-7759
NlmUniqueID: 100965525
Country: United States
Language: English
Volume: 13
Issue: 10
Pages: 772-774

Researcher Affiliations

Barbic, Ljubo
  • 1 Department of Microbiology and Infectious Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb , Zagreb, Croatia .
Vilibic-Cavlek, Tatjana
    Listes, Eddy
      Stevanovic, Vladimir
        Gjenero-Margan, Ira
          Ljubin-Sternak, Suncanica
            Pem-Novosel, Iva
              Listes, Irena
                Mlinaric-Galinovic, Gordana
                  Di Gennaro, Annapia
                    Savini, Giovanni

                      MeSH Terms

                      • Animals
                      • Antibodies, Neutralizing
                      • Antibodies, Viral / blood
                      • Croatia / epidemiology
                      • Cross Reactions
                      • Encephalitis Viruses, Japanese / genetics
                      • Encephalitis Viruses, Japanese / immunology
                      • Encephalitis Viruses, Japanese / isolation & purification
                      • Encephalitis, Arbovirus / epidemiology
                      • Encephalitis, Arbovirus / veterinary
                      • Encephalitis, Arbovirus / virology
                      • Enzyme-Linked Immunosorbent Assay / veterinary
                      • Flavivirus Infections / epidemiology
                      • Flavivirus Infections / veterinary
                      • Flavivirus Infections / virology
                      • Horse Diseases / epidemiology
                      • Horse Diseases / virology
                      • Horses
                      • Humans
                      • Neutralization Tests / veterinary
                      • Seroepidemiologic Studies
                      • West Nile Fever / epidemiology
                      • West Nile Fever / veterinary
                      • West Nile Fever / virology
                      • West Nile virus / immunology
                      • West Nile virus / isolation & purification

                      Citations

                      This article has been cited 28 times.
                      1. Siljic M, Sehovic R, Jankovic M, Stamenkovic G, Loncar A, Todorovic M, Stanojevic M, Cirkovic V. Evolutionary dynamics of Usutu virus: Worldwide dispersal patterns and transmission dynamics in Europe.. Front Microbiol 2023;14:1145981.
                        doi: 10.3389/fmicb.2023.1145981pubmed: 37032910google scholar: lookup
                      2. Akinsulie OC, Adesola RO, Bakre A, Adebowale OO, Adeleke R, Ogunleye SC, Oladapo IP. Usutu virus: An emerging flavivirus with potential threat to public health in Africa: Nigeria as a case study.. Front Vet Sci 2023;10:1115501.
                        doi: 10.3389/fvets.2023.1115501pubmed: 36875996google scholar: lookup
                      3. Trozzi G, Adjadj NR, Vervaeke M, Matthijs S, Sohier C, De Regge N. Comparison of Serological Methods for Tick-Borne Encephalitis Virus-Specific Antibody Detection in Wild Boar and Sheep: Impact of the Screening Approach on the Estimated Seroprevalence.. Viruses 2023 Feb 6;15(2).
                        doi: 10.3390/v15020459pubmed: 36851673google scholar: lookup
                      4. Cavalleri JV, Korbacska-Kutasi O, Leblond A, Paillot R, Pusterla N, Steinmann E, Tomlinson J. European College of Equine Internal Medicine consensus statement on equine flaviviridae infections in Europe.. J Vet Intern Med 2022 Nov;36(6):1858-1871.
                        doi: 10.1111/jvim.16581pubmed: 36367340google scholar: lookup
                      5. Bergmann F, Trachsel DS, Stoeckle SD, Bernis Sierra J, Lübke S, Groschup MH, Gehlen H, Ziegler U. Seroepidemiological Survey of West Nile Virus Infections in Horses from Berlin/Brandenburg and North Rhine-Westphalia, Germany.. Viruses 2022 Jan 25;14(2).
                        doi: 10.3390/v14020243pubmed: 35215837google scholar: lookup
                      6. de Heus P, Kolodziejek J, Hubálek Z, Dimmel K, Racher V, Nowotny N, Cavalleri JV. West Nile Virus and Tick-Borne Encephalitis Virus Are Endemic in Equids in Eastern Austria.. Viruses 2021 Sep 19;13(9).
                        doi: 10.3390/v13091873pubmed: 34578454google scholar: lookup
                      7. Bażanów B, Pawęska JT, Pogorzelska A, Florek M, Frącka A, Gębarowski T, Chwirot W, Stygar D. Serological Evidence of Common Equine Viral Infections in a Semi-Isolated, Unvaccinated Population of Hucul Horses.. Animals (Basel) 2021 Jul 30;11(8).
                        doi: 10.3390/ani11082261pubmed: 34438717google scholar: lookup
                      8. Klobucar A, Savic V, Curman Posavec M, Petrinic S, Kuhar U, Toplak I, Madic J, Vilibic-Cavlek T. Screening of Mosquitoes for West Nile Virus and Usutu Virus in Croatia, 2015-2020.. Trop Med Infect Dis 2021 Apr 2;6(2).
                        doi: 10.3390/tropicalmed6020045pubmed: 33918386google scholar: lookup
                      9. Vilibic-Cavlek T, Barbic L, Mrzljak A, Brnic D, Klobucar A, Ilic M, Janev-Holcer N, Bogdanic M, Jemersic L, Stevanovic V, Tabain I, Krcmar S, Vucelja M, Prpic J, Boljfetic M, Jelicic P, Madic J, Ferencak I, Savic V. Emerging and Neglected Viruses of Zoonotic Importance in Croatia.. Pathogens 2021 Jan 15;10(1).
                        doi: 10.3390/pathogens10010073pubmed: 33467617google scholar: lookup
                      10. Constant O, Bollore K, Clé M, Barthelemy J, Foulongne V, Chenet B, Gomis D, Virolle L, Gutierrez S, Desmetz C, Moares RA, Beck C, Lecollinet S, Salinas S, Simonin Y. Evidence of Exposure to USUV and WNV in Zoo Animals in France.. Pathogens 2020 Nov 30;9(12).
                        doi: 10.3390/pathogens9121005pubmed: 33266071google scholar: lookup
                      11. Benzarti E, Garigliany M. In Vitro and In Vivo Models to Study the Zoonotic Mosquito-Borne Usutu Virus.. Viruses 2020 Sep 30;12(10).
                        doi: 10.3390/v12101116pubmed: 33008141google scholar: lookup
                      12. Schvartz G, Tirosh-Levy S, Erester O, Shenhar R, Levy H, Bazanow B, Gelman B, Steinman A. Exposure of Horses in Israel to West Nile Virus and Usutu Virus.. Viruses 2020 Sep 28;12(10).
                        doi: 10.3390/v12101099pubmed: 32998459google scholar: lookup
                      13. Vilibic-Cavlek T, Petrovic T, Savic V, Barbic L, Tabain I, Stevanovic V, Klobucar A, Mrzljak A, Ilic M, Bogdanic M, Benvin I, Santini M, Capak K, Monaco F, Listes E, Savini G. Epidemiology of Usutu Virus: The European Scenario.. Pathogens 2020 Aug 26;9(9).
                        doi: 10.3390/pathogens9090699pubmed: 32858963google scholar: lookup
                      14. Bakhshi H, Beck C, Lecollinet S, Monier M, Mousson L, Zakeri S, Raz A, Arzamani K, Nourani L, Dinparast-Djadid N, Failloux AB. Serological evidence of West Nile virus infection among birds and horses in some geographical locations of Iran.. Vet Med Sci 2021 Jan;7(1):204-209.
                        doi: 10.1002/vms3.342pubmed: 32858762google scholar: lookup
                      15. Vilibic-Cavlek T, Savic V, Petrovic T, Toplak I, Barbic L, Petric D, Tabain I, Hrnjakovic-Cvjetkovic I, Bogdanic M, Klobucar A, Mrzljak A, Stevanovic V, Dinjar-Kujundzic P, Radmanic L, Monaco F, Listes E, Savini G. Emerging Trends in the Epidemiology of West Nile and Usutu Virus Infections in Southern Europe.. Front Vet Sci 2019;6:437.
                        doi: 10.3389/fvets.2019.00437pubmed: 31867347google scholar: lookup
                      16. Roesch F, Fajardo A, Moratorio G, Vignuzzi M. Usutu Virus: An Arbovirus on the Rise.. Viruses 2019 Jul 12;11(7).
                        doi: 10.3390/v11070640pubmed: 31336826google scholar: lookup
                      17. Zannoli S, Sambri V. West Nile Virus and Usutu Virus Co-Circulation in Europe: Epidemiology and Implications.. Microorganisms 2019 Jun 26;7(7).
                        doi: 10.3390/microorganisms7070184pubmed: 31248051google scholar: lookup
                      18. Beck C, Leparc-Goffart I, Desoutter D, Debergé E, Bichet H, Lowenski S, Dumarest M, Gonzalez G, Migné C, Vanhomwegen J, Zientara S, Durand B, Lecollinet S. Serological evidence of infection with dengue and Zika viruses in horses on French Pacific Islands.. PLoS Negl Trop Dis 2019 Feb;13(2):e0007162.
                        doi: 10.1371/journal.pntd.0007162pubmed: 30730887google scholar: lookup
                      19. Cheng Y, Tjaden NB, Jaeschke A, Lühken R, Ziegler U, Thomas SM, Beierkuhnlein C. Evaluating the risk for Usutu virus circulation in Europe: comparison of environmental niche models and epidemiological models.. Int J Health Geogr 2018 Oct 12;17(1):35.
                        doi: 10.1186/s12942-018-0155-7pubmed: 30314528google scholar: lookup
                      20. Cook CL, Huang YS, Lyons AC, Alto BW, Unlu I, Higgs S, Vanlandingham DL. North American Culex pipiens and Culex quinquefasciatus are competent vectors for Usutu virus.. PLoS Negl Trop Dis 2018 Aug;12(8):e0006732.
                        doi: 10.1371/journal.pntd.0006732pubmed: 30118480google scholar: lookup
                      21. Bażanów B, Jansen van Vuren P, Szymański P, Stygar D, Frącka A, Twardoń J, Kozdrowski R, Pawęska JT. A Survey on West Nile and Usutu Viruses in Horses and Birds in Poland.. Viruses 2018 Feb 17;10(2).
                        doi: 10.3390/v10020087pubmed: 29462983google scholar: lookup
                      22. Lühken R, Jöst H, Cadar D, Thomas SM, Bosch S, Tannich E, Becker N, Ziegler U, Lachmann L, Schmidt-Chanasit J. Distribution of Usutu Virus in Germany and Its Effect on Breeding Bird Populations.. Emerg Infect Dis 2017 Dec;23(12):1994-2001.
                        doi: 10.3201/eid2312.171257pubmed: 29148399google scholar: lookup
                      23. Salinas S, Constant O, Desmetz C, Barthelemy J, Lemaitre JM, Milhavet O, Nagot N, Foulongne V, Perrin FE, Saiz JC, Lecollinet S, Van de Perre P, Simonin Y. Deleterious effect of Usutu virus on human neural cells.. PLoS Negl Trop Dis 2017 Sep;11(9):e0005913.
                        doi: 10.1371/journal.pntd.0005913pubmed: 28873445google scholar: lookup
                      24. Fros JJ, Miesen P, Vogels CB, Gaibani P, Sambri V, Martina BE, Koenraadt CJ, van Rij RP, Vlak JM, Takken W, Pijlman GP. Comparative Usutu and West Nile virus transmission potential by local Culex pipiens mosquitoes in north-western Europe.. One Health 2015 Dec;1:31-36.
                        doi: 10.1016/j.onehlt.2015.08.002pubmed: 28616462google scholar: lookup
                      25. Santini M, Vilibic-Cavlek T, Barsic B, Barbic L, Savic V, Stevanovic V, Listes E, Di Gennaro A, Savini G. First cases of human Usutu virus neuroinvasive infection in Croatia, August-September 2013: clinical and laboratory features.. J Neurovirol 2015 Feb;21(1):92-7.
                        doi: 10.1007/s13365-014-0300-4pubmed: 25361698google scholar: lookup
                      26. Merino-Ramos T, Blázquez AB, Escribano-Romero E, Cañas-Arranz R, Sobrino F, Saiz JC, Martín-Acebes MA. Protection of a single dose west nile virus recombinant subviral particle vaccine against lineage 1 or 2 strains and analysis of the cross-reactivity with Usutu virus.. PLoS One 2014;9(9):e108056.
                        doi: 10.1371/journal.pone.0108056pubmed: 25229345google scholar: lookup
                      27. Vilibic-Cavlek T, Kaic B, Barbic L, Pem-Novosel I, Slavic-Vrzic V, Lesnikar V, Kurecic-Filipovic S, Babic-Erceg A, Listes E, Stevanovic V, Gjenero-Margan I, Savini G. First evidence of simultaneous occurrence of West Nile virus and Usutu virus neuroinvasive disease in humans in Croatia during the 2013 outbreak.. Infection 2014 Aug;42(4):689-95.
                        doi: 10.1007/s15010-014-0625-1pubmed: 24793998google scholar: lookup
                      28. Beck C, Jimenez-Clavero MA, Leblond A, Durand B, Nowotny N, Leparc-Goffart I, Zientara S, Jourdain E, Lecollinet S. Flaviviruses in Europe: complex circulation patterns and their consequences for the diagnosis and control of West Nile disease.. Int J Environ Res Public Health 2013 Nov 12;10(11):6049-83.
                        doi: 10.3390/ijerph10116049pubmed: 24225644google scholar: lookup
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