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Anatomia, histologia, embryologia2004; 33(3); 168-171; doi: 10.1111/j.1439-0264.2004.00529.x

Desmoplakin and Plakoglobin–specific markers of lymphatic vessels in the skin?

Fedele, C
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Berens, D
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Rautenfeld, V
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Pabst, R

Abstract: Monoclonal antibodies against Desmoplakin and Plakoglobin were tested for their suitability as specific markers of lymphatic vessels. The tissue samples were taken from horse skin in an attempt to establish the horse as a model for human lymphatic diseases. To obtain a clear, positive identification of blood and lymphatic vessels, immunohistochemical staining with antibodies against vascular endothelial growth factor receptor 3 (VEGFR-3) and platelet endothelial adhesion molecule (PECAM-1, CD31), was compared with Desmoplakin and Plakoglobin. Because anti-VEGFR-3 is specific for lymphatic vessels in the skin while anti-CD31 stains blood and lymphatic vessels as well, it can be concluded that VEGFR-3(-)/CD31(+) vessels are blood vessels and VEGFR-3(+)/CD31(+) vessels are lymphatic vessels. It was documented on serial sections that Plakoglobin stains both blood and lymphatic vessels. However, Desmoplakin did not stain several positively identified lymphatic vessels. Therefore, Desmoplakin and Plakoglobin antibodies are not specific markers of lymphatic vessels in the skin and the staining pattern is tissues and species dependent.

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Publication Date: 2004-05-18 PubMed ID: 15144286DOI: 10.1111/j.1439-0264.2004.00529.xGoogle Scholar: Lookup

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Comparative Study
Journal Article
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Non-U.S. Gov't

Summary

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This research explores the proposition of Desmoplakin and Plakoglobin as specific markers for lymphatic vessels in skin tissue, through comparative testing on horse skin samples. The study presents evidence that disproves this proposition, suggesting instead that these antibodies aren’t exclusive to lymphatic vessels, and their staining pattern depends on the tissue type and species.

Objective and Methodology

The researchers aimed to test whether antibodies against Desmoplakin and Plakoglobin could serve as specific markers of lymphatic vessels.
They used tissue samples from horse skin, with hopes to establish horse as a model for studying human lymphatic diseases.
The antibodies were tested through immunohistochemical staining and compared with antibodies against vascular endothelial growth factor receptor 3 (VEGFR-3) and platelet endothelial adhesion molecule (PECAM-1, CD31).

Immunohistochemical Staining

Immunohistochemical staining was conducted to differentiate between blood and lymphatic vessels.
Anti-VEGFR-3 was used for identifying lymphatic vessels in the skin, whilst anti-CD31 was used to stain both blood and lymphatic vessels.
This allowed them to deduce that VEGFR-3(-)/CD31(+) vessels are blood vessels while VEGFR-3(+)/CD31(+) vessels are lymphatic vessels.