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Home / Equine Research Bank / Int J Mol Sci / Detection and Genotypic Analysis of Anaplasma bovis and A. p...
International journal of molecular sciences2023; 24(4); 3239; doi: 10.3390/ijms24043239

Detection and Genotypic Analysis of Anaplasma bovis and A. phagocytophilum in Horse Blood and Lung Tissue.

Abstract: A clinical case of was reported for the first time in our previous study (2019) in a horse, a nondefinitive host. Although is a ruminant and not a zoonotic pathogen, it is responsible for persistent infections in horses. In this follow-up study, the prevalence of spp., including , was assessed in horse blood and lung tissue samples to fully understand spp. pathogen distribution and the potential risk factors of infection. Among 1696 samples, including 1433 blood samples from farms nationwide and 263 lung tissue samples from horse abattoirs on Jeju Island, a total of 29 samples (1.7%) tested positive for and 31 (1.8%) samples tested positive for , as determined by 16S rRNA nucleotide sequencing and restriction fragment length polymorphism. This study is the first to detect infection in horse lung tissue samples. Further studies are needed to clarify the comparison of sample types within cohorts. Although the clinical significance of infection was not evaluated in this study, our results emphasize the need to clarify the host tropism and genetic divergence of to enable the development of effective prevention and control measures through broad epidemiological studies.
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Publication Date: 2023-02-07 PubMed ID: 36834651PubMed Central: PMC9966372DOI: 10.3390/ijms24043239Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research involves the genotypic analysis and detection of Anaplasma bovis and A. phagocytophilum in horse blood and lung tissue, with the goal of understanding the distribution of these pathogen species and assessing potential infection risks.

Objective of the Research

  • The researchers aimed to understand the distribution of Anaplasma pathogens in horse blood and lung tissue.
  • The investigation additionally sought to assess the risk factors of acquiring infection from these pathogens.

Methodology

  • A total of 1696 samples were collected, consisting of 1433 blood samples from horses nationwide and 263 lung tissue samples from horse abattoirs on Jeju Island.
  • The samples were tested for the presence of Anaplasma bovis and A. phagocytophilum using 16S rRNA nucleotide sequencing and restriction fragment length polymorphism.

Findings

  • Out of the total samples, 29 (1.7%) tested positive for A. bovis and 31 (1.8%) samples tested positive for A. phagocytophilum.
  • This research represents the first instance of A. bovis infection being detected in horse lung tissue samples.

Significance

  • While the clinical implications of A. bovis infection was not evaluated in this study, the findings highlight the need for further studies to understand the host tropism and genetic divergence of these pathogens.
  • By achieving a better understanding of the pathogens, more effective prevention and control measures could be developed.
  • In concluding their study, the authors assert the need for larger epidemiological studies to confirm and further explore their findings.

Cite This Article

APA
Seo MG, Ouh IO, Kwak D. (2023). Detection and Genotypic Analysis of Anaplasma bovis and A. phagocytophilum in Horse Blood and Lung Tissue. Int J Mol Sci, 24(4), 3239. https://doi.org/10.3390/ijms24043239

Publication

International journal of molecular sciences
ISSN: 1422-0067
NlmUniqueID: 101092791
Country: Switzerland
Language: English
Volume: 24
Issue: 4
PII: 3239

Researcher Affiliations

Seo, Min-Goo
  • College of Veterinary Medicine, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu 41566, Republic of Korea.
Ouh, In-Ohk
  • National Institute of Health, Korea Disease Control and Prevention Agency, 212 Osongsaengmyeong 2-ro, Cheongju, Chungbuk 28160, Republic of Korea.
Kwak, Dongmi
  • College of Veterinary Medicine, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu 41566, Republic of Korea.

MeSH Terms

  • Animals
  • Horses / genetics
  • RNA, Ribosomal, 16S / genetics
  • Follow-Up Studies
  • Anaplasma
  • Anaplasmosis / diagnosis
  • Anaplasmosis / epidemiology
  • Ruminants
  • DNA, Bacterial / genetics
  • Phylogeny

Grant Funding

  • NRF-2016R1D1A1B02015366 / National Research Foundation of Korea

Conflict of Interest Statement

The authors declare no conflict of interest.

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Citations

This article has been cited 1 times.
  1. Seo MG, Lee H, Alkathiri B, Ahn K, Lee SH, Shin S, Bae S, Kim KT, Jang M, Lee SK, Cho YS, Eo KY, Kwon OD, Kwak D. Tick Populations and Molecular Analysis of Anaplasma Species in Ticks from the Republic of Korea.. Microorganisms 2023 Mar 23;11(4).
    doi: 10.3390/microorganisms11040820pubmed: 37110242google scholar: lookup
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