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Journal of virological methods2013; 198; 18-25; doi: 10.1016/j.jviromet.2013.12.008

Detection and quantitation of equid gammaherpesviruses (EHV-2, EHV-5) in nasal swabs using an accredited standardised quantitative PCR method.

Abstract: Equid gammaherpesviruses-2 and -5 are involved in respiratory problems, with potential clinical manifestations such as nasal discharge, pharyngitis and swollen lymph nodes. These viruses are sometimes associated with a poor-performance syndrome, which may result in a significant and negative economic impact for the horse industry. The aim of the present study was to develop and validate quantitative PCR methods for the detection and quantitation of EHV-2 and EHV-5 in equine respiratory fluids. Two distinct tests were characterised: (a) for the qPCR alone and (b) for the whole method (extraction and qPCR) according to the standard model AFNOR XP U47-600-2 (viz., specificity, quantifiable sensibility, linearity, accuracy, range of application, trueness, precision, repeatability and precision of reproducibility). EHV-2 and EHV-5 detection were performed on nasal swabs collected from 172 horses, all of which exhibited clinical signs of respiratory disease. The data revealed a high rate of EHV-2/EHV-5 co-detection that was correlated significantly with age. Viral load of EHV-2 was significantly higher in young horses whereas viral load of EHV-5 was not significantly different with age.
Copyright © 2013 Elsevier B.V. All rights reserved.
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Publication Date: 2013-12-24 PubMed ID: 24370678DOI: 10.1016/j.jviromet.2013.12.008Google Scholar: Lookup
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study focused on developing a set of quantitative PCR tests for the detection and quantification of Equid gammaherpesviruses-2 and -5 in horse respiratory fluids. These viruses are implicated in respiratory diseases in horses and can have economic implications for the horse industry.

Objective of the Study

  • The main objective of this research was to create and validate quantitative PCR tests for detecting and measuring the levels of EHV-2 and EHV-5 in the respiratory fluids of horses.

Methods Used

  • Two distinct tests were characterised in the study: one was for the qPCR method alone and the other included the entire method of extraction and qPCR.
  • The study used the standard model AFNOR XP U47-600-2, which assessed a range of factors such as specificity, measurable sensitivity, linearity, precision, range of application, and so on.

Sampling

  • Nasal swabs were collected from 172 horses, all exhibiting signs of respiratory disease, and were tested for EHV-2 and EHV-5.

Findings

  • The results revealed a high rate of co-detection of EHV-2 and EHV-5, which was significantly correlated with age.
  • It was found that the viral load of EHV-2 was significantly higher in young horses, while the viral load of EHV-5 did not show a significant difference with age.

Implications of the Study

  • The high co-detection rate of EHV-2 and EHV-5, as well as the higher viral load of EHV-2 in young horses, signifies the potential for a high impact on horses’ health—particularly young ones—and consequently, on the economic aspects of the horse industry.
  • This study can help in early detection and management of these viruses, potentially reducing the negative impact on the horse industry.

Cite This Article

APA
Hue ES, Fortier GD, Fortier CI, Leon AM, Richard EA, Legrand LJ, Pronost SL. (2013). Detection and quantitation of equid gammaherpesviruses (EHV-2, EHV-5) in nasal swabs using an accredited standardised quantitative PCR method. J Virol Methods, 198, 18-25. https://doi.org/10.1016/j.jviromet.2013.12.008

Publication

Journal of virological methods
ISSN: 1879-0984
NlmUniqueID: 8005839
Country: Netherlands
Language: English
Volume: 198
Pages: 18-25

Researcher Affiliations

Hue, Erika S
  • Frank Duncombe Laboratory-LABÉO, 1 route de Rosel, 14053 Caen Cedex 4, France; Normandie Universite, 14000 Caen, France; Unite Risques Microbiens (U2RM), EA 4655, 14032 Caen, France; Hippolia Foundation, La Maison du cheval, 6 avenue du Maréchal Montgomery, 14000 Caen, France. Electronic address: Erika.Hue@calvados.fr.
Fortier, Guillaume D
  • Frank Duncombe Laboratory-LABÉO, 1 route de Rosel, 14053 Caen Cedex 4, France; Normandie Universite, 14000 Caen, France; Unite Risques Microbiens (U2RM), EA 4655, 14032 Caen, France; Hippolia Foundation, La Maison du cheval, 6 avenue du Maréchal Montgomery, 14000 Caen, France. Electronic address: Guillaume.Fortier@calvados.fr.
Fortier, Christine I
  • Frank Duncombe Laboratory-LABÉO, 1 route de Rosel, 14053 Caen Cedex 4, France; Hippolia Foundation, La Maison du cheval, 6 avenue du Maréchal Montgomery, 14000 Caen, France. Electronic address: Christine.Fortier@calvados.fr.
Leon, Albertine M
  • Frank Duncombe Laboratory-LABÉO, 1 route de Rosel, 14053 Caen Cedex 4, France; Normandie Universite, 14000 Caen, France; Unite Risques Microbiens (U2RM), EA 4655, 14032 Caen, France; Hippolia Foundation, La Maison du cheval, 6 avenue du Maréchal Montgomery, 14000 Caen, France. Electronic address: Albertine.Leon@calvados.fr.
Richard, Eric A
  • Frank Duncombe Laboratory-LABÉO, 1 route de Rosel, 14053 Caen Cedex 4, France; Hippolia Foundation, La Maison du cheval, 6 avenue du Maréchal Montgomery, 14000 Caen, France. Electronic address: Eric.Richard@calvados.fr.
Legrand, Loïc J
  • Frank Duncombe Laboratory-LABÉO, 1 route de Rosel, 14053 Caen Cedex 4, France; Normandie Universite, 14000 Caen, France; Unite Risques Microbiens (U2RM), EA 4655, 14032 Caen, France; Hippolia Foundation, La Maison du cheval, 6 avenue du Maréchal Montgomery, 14000 Caen, France. Electronic address: Loic.Legrand@calvados.fr.
Pronost, Stéphane L
  • Frank Duncombe Laboratory-LABÉO, 1 route de Rosel, 14053 Caen Cedex 4, France; Normandie Universite, 14000 Caen, France; Unite Risques Microbiens (U2RM), EA 4655, 14032 Caen, France; Hippolia Foundation, La Maison du cheval, 6 avenue du Maréchal Montgomery, 14000 Caen, France. Electronic address: Stephane.Pronost@calvados.fr.

MeSH Terms

  • Animals
  • Gammaherpesvirinae / genetics
  • Herpesviridae Infections / virology
  • Horse Diseases / virology
  • Horses / virology
  • Nose / virology
  • Polymerase Chain Reaction / methods
  • Reproducibility of Results
  • Respiratory Tract Diseases / virology
  • Viral Load / genetics

Citations

This article has been cited 18 times.
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