Detection, molecular characterization and phylogenetic analysis of G3P[12] and G14P[12] equine rotavirus strains co-circulating in central Kentucky.
Abstract: Equine rotavirus A (ERVA) is the leading cause of diarrhea in neonatal foals and a major health problem to the equine breeding industry worldwide. The G3P[12] and G14P[12] ERVA genotypes are the most prevalent in foals with diarrhea. Control and prevention strategies include vaccination of pregnant mares with an inactivated vaccine containing a prototype ERVA G3P[12] strain with limited and controversial field efficacy. Here, we performed the molecular characterization of ERVA strains circulating in central Kentucky using fecal samples collected during the 2017 foaling season. The data indicated for the first time that the G14P[12] genotype is predominant in this region in contrast to a previous serotyping study where only G3 genotype strains were reported. Overall, analysis of antigenic sites in the VP7 protein demonstrated the presence of several amino acid substitutions in the epitopes exposed on the surface including a non-conserved N-linked glycosylation site (D123N) in G14P[12] strains, while changes in antigenic sites of VP8* were minor. Also, we report the successful isolation of three ERVA G14P[12] strains which presented a high identity with other G14 strains from around the world. These may constitute ideal reference strains to comparatively study the molecular biology of G3 and G14 strains and perform vaccine efficacy studies following heterologous challenge in the future.
Copyright © 2018 Elsevier B.V. All rights reserved.
Publication Date: 2018-06-01 PubMed ID: 29864502DOI: 10.1016/j.virusres.2018.05.025Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research focuses on exploring the different strains of Equine rotavirus A (ERVA), a prominent cause of diarrhea in young foals, present in Central Kentucky. It discovered that the G14P[12] strain is more dominant than previously recorded G3 genotype strains.
Research Introduction and Context
- ERVA, a major health issue for the global equine breeding industry, is responsible for severe diarrhea in newborn foals. The ERVA genotypes G3P[12] and G14P[12] are most prevalent in diarrheal foals.
- The current preventive measures involve the vaccination of pregnant mares using an inactivated vaccine containing a prototype ERVA G3P[12] strain. However, its field effectiveness is skeptical and constrained.
Molecular Characterization of ERVA Strains
- The research team performed a molecular characterization of the ERVA strains in Central Kentucky by examining fecal samples obtained during the 2017 foaling season.
- The findings showed that G14P[12] genotype was more prominent in this region, contrary to the earlier studies that only reported G3 genotype strains.
Analysis of Antigenic Sites in Proteins
- Analysis of the antigenic sites in the VP7 protein showed several amino acid substitutions in the epitopes exposed on the surface, including a non-conserved N-linked glycosylation site (D123N) in G14P[12] strains.
- However, changes in the antigenic sites of VP8* were minor.
Identification and Isolation of ERVA G14P[12] Strains
- The team successfully isolated three G14P[12] strains of ERVA. These strains showed high identity with other G14 strains found globally.
- The isolated strains could provide ideal reference points for comparative study of the molecular biology of G3 and G14 strains and potentially serve in future vaccine efficacy studies following heterologous challenge.
Cite This Article
APA
Carossino M, Barrandeguy ME, Li Y, Parreño V, Janes J, Loynachan AT, Balasuriya UBR.
(2018).
Detection, molecular characterization and phylogenetic analysis of G3P[12] and G14P[12] equine rotavirus strains co-circulating in central Kentucky.
Virus Res, 255, 39-54.
https://doi.org/10.1016/j.virusres.2018.05.025 Publication
Researcher Affiliations
- Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY, USA; Escuela de Veterinaria, Universidad del Salvador, Champagnat 1599, Ruta Panamericana km54.5 (B1630AHU), Pilar, Buenos Aires, Argentina.
- Instituto de Virología, CICVyA, INTA. Las Cabañas y Los Reseros s/n, 1712, Castelar, Buenos Aires, Argentina; Escuela de Veterinaria, Universidad del Salvador, Champagnat 1599, Ruta Panamericana km54.5 (B1630AHU), Pilar, Buenos Aires, Argentina.
- Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY, USA.
- Instituto de Virología, CICVyA, INTA. Las Cabañas y Los Reseros s/n, 1712, Castelar, Buenos Aires, Argentina.
- University of Kentucky Veterinary Diagnostic Laboratory, Department of Veterinary Science, University of Kentucky, Lexington, KY, USA.
- University of Kentucky Veterinary Diagnostic Laboratory, Department of Veterinary Science, University of Kentucky, Lexington, KY, USA.
- Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY, USA. Electronic address: balasuriya1@lsu.edu.
MeSH Terms
- Animals
- Antigens, Viral / chemistry
- Antigens, Viral / genetics
- Base Sequence
- Capsid Proteins / chemistry
- Capsid Proteins / genetics
- Cell Line
- Diarrhea / virology
- Feces / virology
- Female
- Genome, Viral / genetics
- Genotype
- Horse Diseases / blood
- Horse Diseases / pathology
- Horse Diseases / virology
- Horses / virology
- Kentucky
- Phylogeny
- Pregnancy
- RNA, Viral / genetics
- RNA-Binding Proteins / chemistry
- RNA-Binding Proteins / genetics
- Rotavirus / classification
- Rotavirus / genetics
- Rotavirus / immunology
- Rotavirus Infections / blood
- Rotavirus Infections / pathology
- Rotavirus Infections / veterinary
- Rotavirus Infections / virology
- Sequence Analysis, DNA
- Viral Nonstructural Proteins / chemistry
- Viral Nonstructural Proteins / genetics
- Viral Proteins / chemistry
- Viral Proteins / genetics
Citations
This article has been cited 11 times.- Borba KER, Legere RM, Canaday NM, Skrobarczyk JW, Arnold ZWT, Cotton-Betteridge E, Poveda C, Criscitiello MF, Bordin AI, Berghman LR, Pollet JBK, Cohen ND. Maternal Immunization with VP8* mRNA Vaccine Yields Superior Passive Transfer of Rotavirus-Neutralizing Antibodies to Foals. Vaccines (Basel) 2026 Jan 9;14(1).
- Gamage C, Holl W, Parreño V, Thieulent CJ, Balasuriya UBR, Vissani MA, Barrandeguy ME, Carossino M. Comparative clinical, virological and pathological characterization of equine rotavirus A G3P[12] and G14P[12] infection in neonatal mice. J Gen Virol 2025 Jun;106(6).
- Cullinane A, Garvey M, Dayot L, Lukaseviciute G. Equine Rotavirus A Outbreaks in Ireland (2023-2024): An Epidemiological Investigation and Virus Genotyping. Viruses 2025 Mar 31;17(4).
- Cullinane A, Nelly M, Dayot L, Lukaseviciute G, Garvey M, Healy J, Gallagher R. Diagnostic Performance of Rapid Antigen Tests to Detect Equine Rotavirus A. Viruses 2025 Mar 14;17(3).
- Ghonaim AH, Rouby SR, Nageeb WM, Elgendy AA, Xu R, Jiang C, Ghonaim NH, He Q, Li W. Insights into recent advancements in human and animal rotavirus vaccines: Exploring new frontiers. Virol Sin 2025 Feb;40(1):1-14.
- Uprety T, Soni S, Sreenivasan C, Hause BM, Naveed A, Ni S, Graves AJ, Morrow JK, Meade N, Mellits KH, Adam E, Kennedy MA, Wang D, Li F. Genetic and antigenic characterization of two diarrhoeicdominant rotavirus A genotypes G3P[12] and G14P[12] circulating in the global equine population. J Gen Virol 2024 Aug;105(8).
- Carossino M, Vissani MA, Barrandeguy ME, Balasuriya UBR, Parreño V. Equine Rotavirus A under the One Health Lens: Potential Impacts on Public Health. Viruses 2024 Jan 16;16(1).
- Carossino M, Balasuriya UBR, Thieulent CJ, Barrandeguy ME, Vissani MA, Parreño V. Quadruplex Real-Time TaqMan(®) RT-qPCR Assay for Differentiation of Equine Group A and B Rotaviruses and Identification of Group A G3 and G14 Genotypes. Viruses 2023 Jul 26;15(8).
- Uprety T, Sreenivasan CC, Hause BM, Li G, Odemuyiwa SO, Locke S, Morgan J, Zeng L, Gilsenan WF, Slovis N, Metcalfe L, Carter CN, Timoney P, Horohov D, Wang D, Erol E, Adam E, Li F. Identification of a Ruminant Origin Group B Rotavirus Associated with Diarrhea Outbreaks in Foals. Viruses 2021 Jul 9;13(7).
- Nemoto M, Matsumura T. Equine rotavirus infection. J Equine Sci 2021 Mar;32(1):1-9.
- Carossino M, Barrandeguy ME, Erol E, Li Y, Balasuriya UBR. Development and evaluation of a one-step multiplex real-time TaqMan(®) RT-qPCR assay for the detection and genotyping of equine G3 and G14 rotaviruses in fecal samples. Virol J 2019 Apr 25;16(1):49.
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