Detection of antibodies to Borna disease virus (BDV) in Turkish horse sera using recombinant p40. Brief report.
Abstract: The nucleoprotein of Borna disease virus (BDV-p40) was produced in a Baculovirus expression system using sf9 cells. The purity and specificity of the recombinant p40 was confirmed by SDS-PAGE and immunoblotting. The recombinant p40 was used in an ELISA to screen horse sera in Turkey. For this, 323 horses from selected cities in the Marmara region of Turkey were examined clinically and serum was collected from each. All horses were clinically healthy except for a few with wounds on the skin. Antibodies to BDV were detected in the sera of 82 (25%) of 323 horse sera. Six sera were selected that had low, medium or high OD values by ELISA and were analysed by Western blotting. All reacted specifically with p40 at a dilution of 1 in 1000. This is the first report of the detection of Borna disease in Turkey and needs further molecular biological investigations to compare the Turkish strains with those strains detected in Europe.
Publication Date: 2002-03-14 PubMed ID: 11890534DOI: 10.1007/s705-002-8331-8Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research article talks about a study conducted for detecting Borna disease virus (BDV) in horses in Turkey. The researchers employed a purified p40 protein, a component of the BDV, to spot the presence of antibodies against this virus in horse serum samples.
Methodology
- To detect the Borna disease virus (BDV), the researchers utilized the nucleoprotein of the virus, known as BDV-p40.
- This BDV-p40 was produced in a Baculovirus expression system, specifically using sf9 cells. An expression system is a system specifically designed for the production of a certain protein. Baculovirus expression system is a popular tool for obtaining eukaryotic proteins in insect cells.
- Following production, the purity and particularity of this BDV-p40 were confirmed through two methods, SDS-PAGE (a common laboratory technique used to separate proteins according to their size) and immunoblotting (a procedure that detects specific proteins within a sample).
ELISA Experiment
- The researchers used the purified BDV-p40 in an ELISA (enzyme-linked immunosorbent assay) to screen horse serum in Turkey. ELISA is a widely used method for detecting and quantifying substances such as peptides, proteins, antibodies, and hormones.
- For the purpose of the study, the researchers collected and examined serum from 323 horses from selected cities in the Marmara region of Turkey. Each horse was clinically evaluated; mostly all were found to be healthy barring a handful that had skin wounds.
Results & Further Testing
- Antibodies to BDV were detected in the sera of 82 (approximately 25%) out of the 323 horse sera.
- Out of these positive samples, six were selected that had low, medium, or high Optical Density (OD) values when tested by ELISA. These samples were further analyzed by Western blotting (a technique used to detect specific proteins in a sample), which confirmed the presence of antibodies against BDV.
- All the selected samples reacted specifically with p40 even at a dilution of 1 in 1000, confirming their robust reactivity and the efficiency of the testing method.
Conclusion
- This research marks the first detection of Borna disease in horses in Turkey. The results open up a pathway for further molecular biological investigations.
- The researchers infer that it would be crucial to compare the Turkish strains of BDV with those previously detected in Europe to enhance our knowledge about their similarities and differences.
Cite This Article
APA
Yilmaz H, Helps CR, Turan N, Uysal A, Harbour DA.
(2002).
Detection of antibodies to Borna disease virus (BDV) in Turkish horse sera using recombinant p40. Brief report.
Arch Virol, 147(2), 429-435.
https://doi.org/10.1007/s705-002-8331-8 Publication
Researcher Affiliations
- University of Istanbul, Veterinary Faculty, Department of Microbiology, Avcilar, Turkey. yilmaz9@hotmail.com
MeSH Terms
- Animals
- Antibodies, Viral / blood
- Baculoviridae / genetics
- Borna Disease / immunology
- Borna Disease / virology
- Borna disease virus / immunology
- Cells, Cultured
- Female
- Horse Diseases / immunology
- Horse Diseases / virology
- Horses
- Male
- Recombinant Proteins / immunology
- Spodoptera
- Turkey
- Viral Proteins / genetics
- Viral Proteins / immunology
Citations
This article has been cited 3 times.- Song JW, Na KS, Tae SH, Kim YK. Borna disease virus antibody and RNA from peripheral blood mononuclear cells of race horses and jockeys in Korea. Psychiatry Investig 2011 Mar;8(1):58-60.
- Bode L, Ludwig H. Borna disease virus infection, a human mental-health risk. Clin Microbiol Rev 2003 Jul;16(3):534-45.
- Hornig M, Briese T, Lipkin WI. Borna disease virus. J Neurovirol 2003 Apr;9(2):259-73.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
