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Home / Equine Research Bank / Vet Sci / Detection of Borrelia burgdorferi Sensu-Lato-Specific Antibo...
Veterinary sciences2022; 9(11); 633; doi: 10.3390/vetsci9110633

Detection of Borrelia burgdorferi Sensu-Lato-Specific Antibodies in Sera of Canine and Equine Origin-A Comparative Study with Two Line Immunoassays.

Abstract: Lyme borreliosis is a vector-borne disease in humans and animals caused by bacteria from the sensu lato complex (sl). The possible transmission of sl from companion animals to humans via ticks makes this disease important in terms of One Health approaches. Thus, early and accurate diagnosis and treatment are of utmost importance. Today's standard for the detection of specific antibodies against sl is a two-tiered test system based on an ELISA for screening combined with a line immunoassay (LIA) for confirmation. In this study, 200 canine and 200 equine serum samples with known antibody status were tested with two different LIAs (A and B). Results were compared regarding sensitivity, specificity, the diagnostic outcome for dogs and horses, as well as operability of the test. The results for canine serum samples corresponded to 94.0%, making both LIAs a good choice for LB diagnostic in dogs. For equine serum samples, the agreement of both tests was 65.5%, displaying the challenge equine samples still provide in LB diagnostic. Major concerns were the interpretation of the OspA antigen (AG) signal and the use of unspecific (i.e., p100/p83) or too sensitive signals on the LIA. The operability of both LIAs was equally user-friendly. Regarding the tests' evaluation, the scanning process provided by LIA A was a major advantage considering the comparability of the tests.
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Publication Date: 2022-11-14 PubMed ID: 36423082PubMed Central: PMC9695075DOI: 10.3390/vetsci9110633Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research is about a comparative study that focuses on the detection of Borrelia burgdorferi sensu lato-specific antibodies in dogs and horses using two different Line Immunoassays. The study aims to understand the effectiveness of these diagnoses in managing Lyme borreliosis, a tick-borne disease, in these animals.

Understanding Lyme Borreliosis and Diagnostic Methods

  • Lyme borreliosis is a disease in humans and animals, transmitted via ticks and caused by bacteria from the Borrelia burgdorferi sensu lato complex.
  • The potential transmission from companion animals like dogs and horses to humans has implications in the field of One Health, which is an integrated approach involving human, animal, and environmental health.
  • Thus, early diagnosis is critical, the standard method for which is a two-tiered test system that involves an ELISA (enzyme-linked immunosorbent assay) for initial screening, followed by a line immunoassay (LIA) for confirmation.

The Comparative Study and Its Results

  • In this study, the researchers tested 200 canine and 200 equine serum samples with known antibody status using two different forms of LIA, referred as Tests A and B.
  • The aim was to compare the sensitivity and specificity of both tests, their diagnostic outcomes for dogs and horses, and how easy they were to operate.
  • Results showed that for dog serum samples, both LIAs showed an agreement of 94.0%, indicating these methods are a competent choice for diagnosing Lyme borreliosis in dogs.

Challenges and Observations

  • However, for horse serum samples, the agreement rate dropped to 65.5%, meaning there are still challenges in diagnosing this disease in horses.
  • One of the major concerns raised was the interpretation of the OspA antigen signal and the use of non-specific signals on the LIA.
  • Yet, both LIAs were found to be equally user-friendly to operate, and Test A’s scanning process was considered advantageous for the comparability of the tests.

Cite This Article

APA
Doff SC, Wenderlein J, Wiesinger A, Hiereth S, Ulrich S, Straubinger RK. (2022). Detection of Borrelia burgdorferi Sensu-Lato-Specific Antibodies in Sera of Canine and Equine Origin-A Comparative Study with Two Line Immunoassays. Vet Sci, 9(11), 633. https://doi.org/10.3390/vetsci9110633

Publication

Veterinary sciences
ISSN: 2306-7381
NlmUniqueID: 101680127
Country: Switzerland
Language: English
Volume: 9
Issue: 11
PII: 633

Researcher Affiliations

Doff, Sophie Charlotte
  • Chair for Bacteriology and Mycology, Institute for Infectious Diseases and Zoonoses, Department of Veterinary Sciences, Faculty of Veterinary Medicine, LMU Munich, Veterinärstr. 13, 80539 Munich, Germany.
Wenderlein, Jasmin
  • Chair for Bacteriology and Mycology, Institute for Infectious Diseases and Zoonoses, Department of Veterinary Sciences, Faculty of Veterinary Medicine, LMU Munich, Veterinärstr. 13, 80539 Munich, Germany.
Wiesinger, Anna
  • Chair for Bacteriology and Mycology, Institute for Infectious Diseases and Zoonoses, Department of Veterinary Sciences, Faculty of Veterinary Medicine, LMU Munich, Veterinärstr. 13, 80539 Munich, Germany.
Hiereth, Stephanie
  • Chair for Bacteriology and Mycology, Institute for Infectious Diseases and Zoonoses, Department of Veterinary Sciences, Faculty of Veterinary Medicine, LMU Munich, Veterinärstr. 13, 80539 Munich, Germany.
Ulrich, Sebastian
  • Chair for Bacteriology and Mycology, Institute for Infectious Diseases and Zoonoses, Department of Veterinary Sciences, Faculty of Veterinary Medicine, LMU Munich, Veterinärstr. 13, 80539 Munich, Germany.
Straubinger, Reinhard K
  • Chair for Bacteriology and Mycology, Institute for Infectious Diseases and Zoonoses, Department of Veterinary Sciences, Faculty of Veterinary Medicine, LMU Munich, Veterinärstr. 13, 80539 Munich, Germany.

Conflict of Interest Statement

The authors declare no conflict of interest.

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