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Home / Equine Research Bank / BMC Vet Res / Detection of equine herpesvirus-4 and physiological stress p...
BMC veterinary research2015; 11; 126; doi: 10.1186/s12917-015-0443-4

Detection of equine herpesvirus-4 and physiological stress patterns in young Thoroughbreds consigned to a South African auction sale.

Abstract: The prevalence of equine herpesvirus types-1 and -4 (EHV-1 and -4) in South African Thoroughbreds at auction sales is currently undefined. Commingling of young Thoroughbreds from various populations together with physiological stress related to their transport and confinement at a sales complex, may be associated with shedding and transmission of EHV-1 and -4. This prospective cohort study sampled 90 young Thoroughbreds consigned from eight farms, originating from three provinces representative of the South African Thoroughbred breeding demographic to a sales complex. Nasal swabs for quantitative real-time polymerase chain reaction (qPCR) assay to detect EHV-1 and -4 nucleic acid and blood samples for enzyme-linked immunosorbent assay for EHV-1 and -4 antibodies were collected from all horses on arrival and departure. Additional nasal swabs for qPCR were obtained serially from those displaying pyrexia and, or nasal discharge. Daily faecal samples were used for determination of faecal glucocorticoid metabolite (FGM) concentrations as a measurement of physiological stress and these values were modelled to determine the factors best explaining FGM variability. Results: EHV-4 nucleic acid was detected in 14.4 % and EHV-1 from none of the animals in the study population. Most (93.3 %) and very few (1.1 %) of this population showed antibodies indicating prior exposure to EHV-4 and EHV-1 respectively. Pyrexia and nasal discharge were poor predictors for detecting EHV-4 nucleic acid. The horses' FGM concentrations increased following arrival before decreasing for most of the remaining study period including the auction process. Model averaging showed that variation in FGM concentrations was best explained by days post-arrival and transport duration. Conclusions: In this study population, sales consignment was associated with limited detection of EHV-4 nucleic acid in nasal secretions, with most showing prior exposure to EHV-4 and very few to EHV-1. The physiological stress response shown by most reflected the combination of stressors associated with transport and arrival and these are key areas for future investigation into management practices to enhance health and welfare of young Thoroughbreds during sales consignment.
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Publication Date: 2015-06-02 PubMed ID: 26033323PubMed Central: PMC4450643DOI: 10.1186/s12917-015-0443-4Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the prevalence of equine herpesvirus types-1 and -4 (EHV-1 and -4) among young Thoroughbred horses brought together for an auction sale in South Africa. The study also explores the correlation between the stress levels of these horses and the occurrence of the viruses.

Objective of Research and Methodology

  • The study aimed to understand the occurrence and transmission of EHV-1 and -4 among young Thoroughbreds that were brought in from different locations for a horse auction. The stress induced by the transportation and confinement of these horses at the auction site was considered as a possible factor contributing to the shedding and transmission of the equine herpesviruses.
  • A cohort of 90 young Thoroughbreds from eight farms across three provinces were included in study, representing the South African Thoroughbred breeding demographic.
  • Nasal swabs for qPCR assay were used to detect the presence of EHV-1 and -4, whereas blood samples were analyzed for EHV-1 and -4 antibodies, collected from all horses on arrival and departure. Horses displaying pyrexia and/or nasal discharge were subjected to additional nasal swabs for qPCR.
  • Daily fecal samples were used to determine the levels of faecal glucocorticoid metabolite (FGM) concentrations, an indicator of physiological stress. FGM values were then modeled to identify factors explaining their variability.

Main Findings

  • In the study population, EHV-4 was present in 14.4% of animals, while EHV-1 was not found in any of the animals.
  • 93.3% of the population demonstrated antibodies against EHV-4, indicating prior exposure to the virus, but EHV-1 antibodies were detected in only 1.1% of the horses.
  • The factors such as pyrexia and nasal discharge were not reliable predictors for EHV-4 presence. The level of FGM increased in horses post arrival, suggesting an increase in stress, but later decreased for most horses, including during the auction process.
  • Variation in FGM concentrations was best explained by days after arrival and duration of transportation, which could be important areas for future investigation to better manage and enhance the welfare of young Thoroughbreds during auctions.

Implications for Future Research

  • While the presence of EHV-4 was detected in limited incidence and was associated with buying and selling of horses, the role of stress in this prevalence remains a subject for future investigations.
  • Understanding the relation between stressors, such as transport and confinement, and the health of young Thoroughbreds is vital for improving management practices and the overall welfare of the horses in sales consignment scenarios.

Cite This Article

APA
Badenhorst M, Page P, Ganswindt A, Laver P, Guthrie A, Schulman M. (2015). Detection of equine herpesvirus-4 and physiological stress patterns in young Thoroughbreds consigned to a South African auction sale. BMC Vet Res, 11, 126. https://doi.org/10.1186/s12917-015-0443-4

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 11
Pages: 126
PII: 126

Researcher Affiliations

Badenhorst, Marcha
  • Department of Companion Animal Clinical Studies, Faculty of Veterinary Science, University of Pretoria, Private Bag XO4, Onderstepoort, 0110, South Africa. marcha.badenhorst@up.ac.za.
Page, Patrick
  • Department of Companion Animal Clinical Studies, Faculty of Veterinary Science, University of Pretoria, Private Bag XO4, Onderstepoort, 0110, South Africa. patrick.page@up.ac.za.
Ganswindt, Andre
  • Department of Anatomy and Physiology, Faculty of Veterinary Science, University of Pretoria, Private Bag XO4, Onderstepoort, 0110, South Africa. aganswindt@zoology.up.ac.za.
Laver, Peter
  • Department of Anatomy and Physiology, Faculty of Veterinary Science, University of Pretoria, Private Bag XO4, Onderstepoort, 0110, South Africa. pnlaver@gmail.com.
Guthrie, Alan
  • Equine Research Centre, Faculty of Veterinary Science, University of Pretoria, Private Bag XO4, Onderstepoort, 0110, South Africa. alan.guthrie@up.ac.za.
Schulman, Martin
  • Section of Reproduction, Department of Production Animal Clinical Studies, Faculty of Veterinary Science, University of Pretoria, Private Bag XO4, Onderstepoort, 0110, South Africa. martin.schulman@up.ac.za.

MeSH Terms

  • Animals
  • Antibodies, Viral / blood
  • Cohort Studies
  • Commerce
  • DNA, Viral / blood
  • DNA, Viral / isolation & purification
  • Feces / chemistry
  • Herpesviridae Infections / epidemiology
  • Herpesviridae Infections / veterinary
  • Herpesviridae Infections / virology
  • Herpesvirus 4, Equid / isolation & purification
  • Hormones / chemistry
  • Horse Diseases / virology
  • Horses
  • South Africa / epidemiology
  • Stress, Physiological

References

This article includes 40 references
  1. Bailey CJ, Rose RJ, Reid SW, Hodgson DR. Wastage in the Australian thoroughbred racing industry: a survey of Sydney trainers.. Aust Vet J 1997 Jan;75(1):64-6.
    doi: 10.1111/j.1751-0813.1997.tb13836.xpubmed: 9034505google scholar: lookup
  2. Slater J. Equine herpesviruses. In: Sellon DC, Long MT, editors. Equine infectious diseases. 1. St Louis: Saunders Elsevier; 2007. pp. 134–53.
  3. Matsumura T, Sugiura T, Imagawa H, Fukunaga Y, Kamada M. Epizootiological aspects of type 1 and type 4 equine herpesvirus infections among horse populations.. J Vet Med Sci 1992 Apr;54(2):207-11.
    doi: 10.1292/jvms.54.207pubmed: 1318750google scholar: lookup
  4. Pusterla N, Kass PH, Mapes S, Johnson C, Barnett DC, Vaala W, Gutierrez C, McDaniel R, Whitehead B, Manning J. Surveillance programme for important equine infectious respiratory pathogens in the USA.. Vet Rec 2011 Jul 2;169(1):12.
    doi: 10.1136/vr.d2157pubmed: 21676986google scholar: lookup
  5. Ko S, Kang J, Yeh J, Moon J, Choi G, Won S. First report on molecular detection of equine upper respiratory infectious viruses in republic of Korea. J Equine Vet Sci 2013;33:628–36.
    doi: 10.1016/j.jevs.2012.11.001google scholar: lookup
  6. Foote CE, Love DN, Gilkerson JR, Whalley JM. Detection of EHV-1 and EHV-4 DNA in unweaned Thoroughbred foals from vaccinated mares on a large stud farm.. Equine Vet J 2004 May;36(4):341-5.
    doi: 10.2746/0425164044890634pubmed: 15163042google scholar: lookup
  7. Wang L, Raidal SL, Pizzirani A, Wilcox GE. Detection of respiratory herpesviruses in foals and adult horses determined by nested multiplex PCR.. Vet Microbiol 2007 Mar 31;121(1-2):18-28.
    doi: 10.1016/j.vetmic.2006.11.009pubmed: 17208393google scholar: lookup
  8. Morley PS, Townsend HG, Bogdan JR, Haines DM. Risk factors for disease associated with influenza virus infections during three epidemics in horses.. J Am Vet Med Assoc 2000 Feb 15;216(4):545-50.
    doi: 10.2460/javma.2000.216.545pubmed: 10687010google scholar: lookup
  9. Lunn DP, Davis-Poynter N, Flaminio MJ, Horohov DW, Osterrieder K, Pusterla N, Townsend HG. Equine herpesvirus-1 consensus statement.. J Vet Intern Med 2009 May-Jun;23(3):450-61.
    doi: 10.1111/j.1939-1676.2009.0304.xpubmed: 19645832google scholar: lookup
  10. Harless W, Pusterla N. Equine herpesvirus 1 and 4 respiratory disease in the horse. Clin Tech Equine Pract 2006;5:197–202.
    doi: 10.1053/j.ctep.2006.03.014google scholar: lookup
  11. Cardwell JM, Smith KC, Wood JL, Newton JR. A longitudinal study of respiratory infections in British National Hunt racehorses.. Vet Rec 2013 Jun 15;172(24):637.
    doi: 10.1136/vr.101520pubmed: 23650184google scholar: lookup
  12. Carlson JK, Traub-Dargatz JL, Lunn DP, Morley PS, Kohler A, Kasper K. Equine viral respiratory pathogen surveillance at horse shows and sales. J Equine Vet Sci 2013;33:229–37.
    doi: 10.1016/j.jevs.2012.06.006google scholar: lookup
  13. Gilkerson JR, Teague N, Whalley JM, Love DN. A prospective cohort study of upper respiratory tract disease in one and two year old racehorses. Serological evaluation of the role of equine herpesviruses 1 and 4 (EHV-1 and EHV-4) in respiratory disease. Aust Equine Vet 1999;17:76–81.
  14. Patel JR, Heldens J. Equine herpesviruses 1 (EHV-1) and 4 (EHV-4)--epidemiology, disease and immunoprophylaxis: a brief review.. Vet J 2005 Jul;170(1):14-23.
    doi: 10.1016/j.tvjl.2004.04.018pubmed: 15993786google scholar: lookup
  15. Pusterla N, Hussey SB, Mapes S, Johnson C, Collier JR, Hill J, Lunn DP, Wilson WD. Molecular investigation of the viral kinetics of equine herpesvirus-1 in blood and nasal secretions of horses after corticosteroid-induced recrudescence of latent infection.. J Vet Intern Med 2010 Sep-Oct;24(5):1153-7.
    doi: 10.1111/j.1939-1676.2010.0554.xpubmed: 20584139google scholar: lookup
  16. Pusterla N, Mapes S, Madigan JE, Maclachlan NJ, Ferraro GL, Watson JL, Spier SJ, Wilson WD. Prevalence of EHV-1 in adult horses transported over long distances.. Vet Rec 2009 Oct 17;165(16):473-5.
    doi: 10.1136/vr.165.16.473pubmed: 19850855google scholar: lookup
  17. Schulman M, Becker A, Ganswindt S, Guthrie A, Stout T, Ganswindt A. The effect of consignment to broodmare sales on physiological stress measured by faecal glucocorticoid metabolites in pregnant Thoroughbred mares.. BMC Vet Res 2014 Jan 17;10:25.
    doi: 10.1186/1746-6148-10-25pmc: PMC3898249pubmed: 24433380google scholar: lookup
  18. Möstl E, Palme R. Hormones as indicators of stress.. Domest Anim Endocrinol 2002 Jul;23(1-2):67-74.
    doi: 10.1016/S0739-7240(02)00146-7pubmed: 12142227google scholar: lookup
  19. Touma C, Palme R. Measuring fecal glucocorticoid metabolites in mammals and birds: the importance of validation.. Ann N Y Acad Sci 2005 Jun;1046:54-74.
    doi: 10.1196/annals.1343.006pubmed: 16055843google scholar: lookup
  20. Schmidt A, Biau S, Möstl E, Becker-Birck M, Morillon B, Aurich J, Faure JM, Aurich C. Changes in cortisol release and heart rate variability in sport horses during long-distance road transport.. Domest Anim Endocrinol 2010 Apr;38(3):179-89.
    doi: 10.1016/j.domaniend.2009.10.002pubmed: 19962266google scholar: lookup
  21. Schmidt A, Möstl E, Wehnert C, Aurich J, Müller J, Aurich C. Cortisol release and heart rate variability in horses during road transport.. Horm Behav 2010 Feb;57(2):209-15.
    doi: 10.1016/j.yhbeh.2009.11.003pubmed: 19944105google scholar: lookup
  22. Diallo IS, Hewitson G, Wright LL, Kelly MA, Rodwell BJ, Corney BG. Multiplex real-time PCR for the detection and differentiation of equid herpesvirus 1 (EHV-1) and equid herpesvirus 4 (EHV-4).. Vet Microbiol 2007 Jul 20;123(1-3):93-103.
    doi: 10.1016/j.vetmic.2007.02.004pubmed: 17346907google scholar: lookup
  23. Crabb BS, Studdert MJ. Epitopes of glycoprotein G of equine herpesviruses 4 and 1 located near the C termini elicit type-specific antibody responses in the natural host.. J Virol 1993 Oct;67(10):6332-8.
    pmc: PMC238063pubmed: 7690425doi: 10.1128/jvi.67.10.6332-6338.1993google scholar: lookup
  24. Gilkerson JR, Whalley JM, Drummer HE, Studdert MJ, Love DN. Epidemiological studies of equine herpesvirus 1 (EHV-1) in Thoroughbred foals: a review of studies conducted in the Hunter Valley of New South Wales between 1995 and 1997.. Vet Microbiol 1999 Aug 16;68(1-2):15-25.
    doi: 10.1016/S0378-1135(99)00057-7pubmed: 10501158google scholar: lookup
  25. Ganswindt A, Muenscher S, Henley M, Palme R, Thompson P, Bertschinger H. Concentrations of faecal glucocorticoid metabolites in physically injured free-ranging African elephants (Loxodonta africana). Wildlife Biol 2010;16:323–32.
    doi: 10.2981/09-081google scholar: lookup
  26. Merl S, Scherzer S, Palme S, Möstl E. Pain causes increased concentrations of glucocorticoid metabolites in horse feces. J Equine Vet Sci 2000;20:586–90.
    doi: 10.1016/S0737-0806(00)70267-Xgoogle scholar: lookup
  27. Schatz S, Palme R. Measurement of faecal cortisol metabolites in cats and dogs: a non-invasive method for evaluating adrenocortical function.. Vet Res Commun 2001 May;25(4):271-87.
    doi: 10.1023/A:1010626608498pubmed: 11432429google scholar: lookup
  28. Heistermann M, Palme R, Ganswindt A. Comparison of different enzyme-immunoassays for assessment of adrenocortical activity in primates based on fecal analysis.. Am J Primatol 2006 Mar;68(3):257-73.
    doi: 10.1002/ajp.20222pubmed: 16477600google scholar: lookup
  29. Palme R, Möstl E. Measurement of cortisol metabolites in faeces of sheep as a parameter of cortisol concentration in blood. Int J Mamml Biol 1997;62:192–7.
  30. Ganswindt A, Heistermann M, Borragan S, Hodges JK. Assessment of testicular endocrine function in captive African elephants by measurement of urinary and fecal androgens. Zoo Biol 2002;21:27–36.
    doi: 10.1002/zoo.10034google scholar: lookup
  31. R Core Team. R: A language and environment for statistical computing. Vienna: R Foundation for Statistical Computing; 2012.
  32. Akaike H. A new look at the statistical model identification. IEEE T Automat Contr 1974;19:716–23.
    doi: 10.1109/TAC.1974.1100705google scholar: lookup
  33. Burnham KP, Anderson DR. Model selection and multimodel inference: a practical information-theoretic approach. 2. New York: Springer; 2002.
  34. Arnold TW. Uninformative parameters and model selection using Akaike’s Information Criterion. J Wildl Manag 2010;74:1175–8.
    doi: 10.1111/j.1937-2817.2010.tb01236.xgoogle scholar: lookup
  35. Xu R. Measuring explained variation in linear mixed effects models.. Stat Med 2003 Nov 30;22(22):3527-41.
    doi: 10.1002/sim.1572pubmed: 14601017google scholar: lookup
  36. Gilkerson JR, Whalley JM, Drummer HE, Studdert MJ, Love DN. Epidemiology of EHV-1 and EHV-4 in the mare and foal populations on a Hunter Valley stud farm: are mares the source of EHV-1 for unweaned foals.. Vet Microbiol 1999 Aug 16;68(1-2):27-34.
    doi: 10.1016/S0378-1135(99)00058-9pubmed: 10501159google scholar: lookup
  37. Aharonson-Raz K, Davidson I, Porat Y, Altory A, Klement E, Steinman A. Seroprevalence and rate of infection of equine influenza virus (H3N3 and H7N7) and equine herpes virus (1 and 4) in the horse population of Israel. J Equine Vet Sci 2014;34:828–32.
    doi: 10.1016/j.jevs.2014.01.012google scholar: lookup
  38. Bell SA, Balasuriya UB, Gardner IA, Barry PA, Wilson WD, Ferraro GL, MacLachlan NJ. Temporal detection of equine herpesvirus infections of a cohort of mares and their foals.. Vet Microbiol 2006 Sep 10;116(4):249-57.
    doi: 10.1016/j.vetmic.2006.05.002pubmed: 16774810google scholar: lookup
  39. Walter J, Seeh C, Fey K, Bleul U, Osterrieder N. Clinical observations and management of a severe equine herpesvirus type 1 outbreak with abortion and encephalomyelitis.. Acta Vet Scand 2013 Mar 5;55(1):19.
    doi: 10.1186/1751-0147-55-19pmc: PMC3630004pubmed: 23497661google scholar: lookup
  40. Pusterla N, Mapes S, Wademan C, White A, Hodzic E. Investigation of the role of lesser characterised respiratory viruses associated with upper respiratory tract infections in horses.. Vet Rec 2013 Mar 23;172(12):315.
    doi: 10.1136/vr.100943pubmed: 23423483google scholar: lookup

Citations

This article has been cited 7 times.
  1. Pacchiarotti G, Nardini R, Scicluna MT. Equine Hepacivirus: A Systematic Review and a Meta-Analysis of Serological and Biomolecular Prevalence and a Phylogenetic Update.. Animals (Basel) 2022 Sep 20;12(19).
    doi: 10.3390/ani12192486pubmed: 36230228google scholar: lookup
  2. Mureşan A, Mureşan C, Siteavu M, Avram E, Bochynska D, Taulescu M. An Outbreak of Equine Herpesvirus-4 in an Ecological Donkey Milk Farm in Romania.. Vaccines (Basel) 2022 Mar 18;10(3).
    doi: 10.3390/vaccines10030468pubmed: 35335100google scholar: lookup
  3. Brown LJ, Brown G, Kydd J, Stout TAE, Schulman ML. Failure to detect equid herpesvirus types 1 and 4 DNA in placentae and healthy new-born Thoroughbred foals.. J S Afr Vet Assoc 2019 May 30;90(0):e1-e5.
    doi: 10.4102/jsava.v90i0.1736pubmed: 31170779google scholar: lookup
  4. Sanz MG, Kwon S, Pusterla N, Gold JR, Bain F, Evermann J. Evaluation of equine coronavirus fecal shedding among hospitalized horses.. J Vet Intern Med 2019 Mar;33(2):918-922.
    doi: 10.1111/jvim.15449pubmed: 30788861google scholar: lookup
  5. Muscat KE, Padalino B, Hartley CA, Ficorilli N, Celi P, Knight P, Raidal S, Gilkerson JR, Muscatello G. Equine Transport and Changes in Equid Herpesvirus' Status.. Front Vet Sci 2018;5:224.
    doi: 10.3389/fvets.2018.00224pubmed: 30320126google scholar: lookup
  6. Seeber PA, Quintard B, Sicks F, Dehnhard M, Greenwood AD, Franz M. Environmental stressors may cause equine herpesvirus reactivation in captive Grévy's zebras (Equus grevyi).. PeerJ 2018;6:e5422.
    doi: 10.7717/peerj.5422pubmed: 30155350google scholar: lookup
  7. Page P, Ganswindt A, Schoeman J, Venter G, Guthrie A. The effect of alphacypermethrin-treated mesh protection against African horse sickness virus vectors on jet stall microclimate, clinical variables and faecal glucocorticoid metabolites of horses.. BMC Vet Res 2017 Sep 9;13(1):283.
    doi: 10.1186/s12917-017-1198-xpubmed: 28886712google scholar: lookup
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