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Home / Equine Research Bank / J Clin Microbiol / Detection of group C rotavirus antigens and antibodies in an...
Journal of clinical microbiology1992; 30(8); 2129-2134; doi: 10.1128/jcm.30.8.2129-2134.1992

Detection of group C rotavirus antigens and antibodies in animals and humans by enzyme-linked immunosorbent assays.

Abstract: Enzyme-linked immunosorbent assays (ELISAs) were developed to detect group (gp) C rotavirus antigens and antibodies. Both assays were confirmed to be specific for gp C rotavirus by using serogroup A, B, and C rotaviruses; hyperimmune antisera to these serogroups of rotaviruses; and paired serum specimens from animals infected with gp C rotaviruses. The ELISA for antigen detection reacted not only with porcine gp C rotaviruses but also with human and bovine gp C rotaviruses. Following experimental challenge of gnotobiotic pigs with porcine gp C rotavirus, the virus was found by ELISA in all diarrheic feces. A high prevalence of antibodies to gp C rotaviruses was detected in sera from adult pigs (93 to 97%) and cattle (47 to 56%) in the United States and Japan. However, no antibody to gp C rotavirus was detected in the sera (n = 20) of adult horses in the United States. In human sera from Hokkaido, Japan, 3% of children and 13% of adults possessed antibody to gp C rotaviruses. These results suggest that the ELISA that we developed may be useful for surveying gp C rotavirus infections in animals and humans. On the basis of serology, gp C rotavirus infections are common in pigs and cattle in the United States and Japan, but they occur at lower levels in humans from the Hokkaido area of Japan.
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Publication Date: 1992-08-01 PubMed ID: 1323577PubMed Central: PMC265456DOI: 10.1128/jcm.30.8.2129-2134.1992Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't
  • Research Support
  • U.S. Gov't
  • Non-P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study developed an Enzyme-Linked Immunosorbent Assays (ELISAs) to detect group (gp) C rotavirus antigens and antibodies and found that these could be a useful tool for surveying gp C rotavirus infections in animals and humans.

Study Design and Experimentation

  • In the study, an enzyme-linked immunosorbent assays (ELISAs) method was developed specifically to detect group C rotavirus antigens and its antibodies.
  • The assay’s specificity for group C was confirmed through tests using serogroups A, B, and C rotaviruses and hyperimmune antisera to these serogroups of rotaviruses.
  • The researchers also tested paired serum specimens from animals infected with group C rotaviruses.
  • Notably, the ELISA for antigen detection reacted with porcine (pig), human, and bovine (cattle) group C rotaviruses, showing its ability to detect this virus across different species.
  • The ELISAs was also used to examine the feces of gnotobiotic pigs, that is, pigs that have been reared in a germ-free environment, which were experimentally infected with porcine group C rotavirus.

Outcome of the Study

  • The researchers found that the virus was present in all the diarrheic feces of the infected pigs.
  • The researchers then used the ELISA method to detect antibodies to group C rotaviruses in serum samples from adult pigs and cattle in the United States and Japan.
  • Results show a high prevalence of antibodies to group C rotaviruses in adult pigs (between 93% to 97%) and cattle (between 47% to 56%) across the two countries.
  • No antibodies to the group C rotavirus was detected in serum samples from adult horses in the U.S.
  • The method was additionally used to check human sera from Hokkaido, Japan; results show that 3% of children and 13% of adults had antibodies to group C rotaviruses.

Conclusion and Implications

  • The researchers suggest that the ELISA they developed could be an effective tool for monitoring group C rotavirus infections in both animals and humans.
  • Based on their findings, it appears that infections from group C rotaviruses are common among pigs and cattle in the United States and Japan.
  • However, compared to the animal populations studied, the infections occur at lower levels in the human population from the Hokkaido area of Japan.

Cite This Article

APA
Tsunemitsu H, Jiang B, Saif LJ. (1992). Detection of group C rotavirus antigens and antibodies in animals and humans by enzyme-linked immunosorbent assays. J Clin Microbiol, 30(8), 2129-2134. https://doi.org/10.1128/jcm.30.8.2129-2134.1992

Publication

Journal of clinical microbiology
ISSN: 0095-1137
NlmUniqueID: 7505564
Country: United States
Language: English
Volume: 30
Issue: 8
Pages: 2129-2134

Researcher Affiliations

Tsunemitsu, H
  • Hokkaido Prefectural Shintoku Animal Husbandry Experiment Station, Japan.
Jiang, B
    Saif, L J

      MeSH Terms

      • Animals
      • Antibodies, Viral / analysis
      • Antigens, Viral / analysis
      • Cattle
      • Cattle Diseases / immunology
      • Enzyme-Linked Immunosorbent Assay / methods
      • Enzyme-Linked Immunosorbent Assay / statistics & numerical data
      • Evaluation Studies as Topic
      • Humans
      • Rotavirus / classification
      • Rotavirus / immunology
      • Rotavirus Infections / immunology
      • Rotavirus Infections / veterinary
      • Sensitivity and Specificity
      • Seroepidemiologic Studies
      • Swine
      • Swine Diseases / immunology
      • Virology / methods

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