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Anatomy and embryology1995; 192(2); 117-121; doi: 10.1007/BF00186000

Detection of immunoreactive atrial and brain natriuretic peptides in the equine atrium.

Abstract: The distribution of immunoreactivity (IR) for cardiodilatin/atrial natriuretic peptide (CDD/ANP) and brain natriuretic peptide (BNP) was examined immunohistochemically and immuno-electron-microscopically in the equine atrium, using specific antibodies. In the immunohistochemical studies, IR-CDD/ANP and IR-pBNP-26 (porcine BNP-26 immunoreactivity) was detected in the cytoplasm of the auricular cardiocytes, but IR-hBNP-32 (human BNP-32 immunoreactivity) was not. The double immunogold labelling method for IR-hBNP-28 and IR-pBNP-26 revealed that gold particles of different sizes were located in the same secretory granules in the cardiocyte, but no gold particles for IR-hBNP-32 were detected. These results show that CDD/ANP and porcine BNP-like peptides are colocalized in the same secretory granules of the equine atrium. They suggest that the equine atrium secretes both CDD/ANP and BNP-like peptides.
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Publication Date: 1995-08-01 PubMed ID: 7486008DOI: 10.1007/BF00186000Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the occurrence and distribution of specific peptides, cardiodilatin/atrial natriuretic peptide (CDD/ANP) and brain natriuretic peptide (BNP), in the heart atrium of horses.

Explanation of the Research Paper

This research focuses on two key cardiac hormones – Atrial Natriuretic Peptide (ANP) and Brain Natriuretic Peptide (BNP) – which are usually produced in heart muscles. The researchers looked into their existence and distribution in the atrium of horses.

  • The first part of the study involved immunohistochemical examination – a way to detect specific proteins in cells of a tissue. Here, they studied the incidence of these hormones in the equine atrium using specific antibodies. They noticed the presence of both CDD/ANP and BNP, specifically a type referred to as IR-pBNP-26 (porcine BNP-26 immunoreactivity), in the auricular cardiocytes (cells of the heart’s upper chambers). However, they were unable to detect IR-hBNP-32 (human BNP-32 immunoreactivity).
  • To further explore, they made use of the double immunogold labelling technique, a method used to study the existence and distribution of the same or different antigens in cells or structural compartments. They found out that distinct sizes of gold particles, used to mark the peptides, were located in the same secretion units of the cardiocyte. Again, no gold particles for IR-hBNP-32 were identified.

Outcomes and Implications of the Study

  • The results clearly demonstrated that CDD/ANP and BNP-like peptides (porcine BNP specifically) coexist in the same secretory cells of the equine atrium.
  • It’s suggested that, much like in humans and perhaps other animals, the atrium of horses also secretes both ANP and BNP-like peptides. This raises questions about the roles they might play in cardiac function, specifically in horses, thereby setting a course for future research in equine cardiac health.

Cite This Article

APA
Mifune H, Richter R, Forssmann WG. (1995). Detection of immunoreactive atrial and brain natriuretic peptides in the equine atrium. Anat Embryol (Berl), 192(2), 117-121. https://doi.org/10.1007/BF00186000

Publication

Anatomy and embryology
ISSN: 0340-2061
NlmUniqueID: 7505194
Country: Germany
Language: English
Volume: 192
Issue: 2
Pages: 117-121

Researcher Affiliations

Mifune, H
  • Niedersächsisches Institut für Peptid-Forschung (IPF), Hannover, Germany.
Richter, R
    Forssmann, W G

      MeSH Terms

      • Animals
      • Atrial Natriuretic Factor / analysis
      • Atrial Natriuretic Factor / immunology
      • Cross Reactions
      • Cytoplasmic Granules / chemistry
      • Heart Atria / chemistry
      • Heart Atria / ultrastructure
      • Horses / metabolism
      • Immunohistochemistry
      • Microscopy, Immunoelectron
      • Muscle Proteins / analysis
      • Muscle Proteins / immunology
      • Natriuretic Peptide, Brain
      • Nerve Tissue Proteins / analysis
      • Nerve Tissue Proteins / immunology

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      Citations

      This article has been cited 2 times.
      1. Öztop M, Özbek M, Liman N, Beyaz F, Ergün E, Ergün L. Localization profiles of natriuretic peptides in hearts of pre-hibernating and hibernating Anatolian ground squirrels (Spermophilus xanthoprymnus). Vet Res Commun 2019 May;43(2):45-65.
        doi: 10.1007/s11259-019-9745-5pubmed: 30689110google scholar: lookup
      2. Nishi Y, Mifune H, Yabuki A, Tajiri Y, Hirata R, Tanaka E, Hosoda H, Kangawa K, Kojima M. Changes in Subcellular Distribution of n-Octanoyl or n-Decanoyl Ghrelin in Ghrelin-Producing Cells. Front Endocrinol (Lausanne) 2013;4:84.
        doi: 10.3389/fendo.2013.00084pubmed: 23847595google scholar: lookup
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