Detection of influenza nucleoprotein antigen in nasal secretions from horses infected with A/equine influenza (H3N8) viruses.
Abstract: An antigen capture indirect enzyme linked immunosorbent assay (ELISA) was developed to detect influenza nucleoprotein antigen in nasal secretions from horses infected with A/equine/H3N8 viruses. Results from this assay were compared with conventional virus isolation in embryonated hens eggs.
Publication Date: 1988-05-01 PubMed ID: 2840448DOI: 10.1016/0166-0934(88)90034-1Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research focused on developing an antigen capture enzyme linked immunosorbent assay (ELISA) to detect influenza nucleoprotein antigen in horses. The new method was compared to conventional virus isolation techniques.
Methodology
- The research involved the use of an antigen capture enzyme linked immunosorbent assay (ELISA).
- The ELISA method was focused on the detection of influenza nucleoprotein antigen, in horses infected with A/equine/H3N8 viruses.
- Nasal secretions from horses were taken as the sample for this assay.
Comparison with Conventional Virus Isolation Techniques
- The result produced by the antigen capture ELISA was compared to that of conventional virus isolation.
- In the conventional method, virus isolation was done in embryonated hens eggs.
- The comparison was conducted to verify the efficacy of the ELISA method against an established method for detecting the influenza virus.
Significance
- The study focused on a more efficient method for the detection of influenza viruses, specifically the A/equine/H3N8 viruses, in horses.
- The potential success of the ELISA detection method could mean faster, easier, and more accurate detection techniques to help manage influenza outbreaks in horses.
- The direct benefit of this research is to potentially improve management strategies for equine influenza.
Cite This Article
APA
Cook RF, Sinclair R, Mumford JA.
(1988).
Detection of influenza nucleoprotein antigen in nasal secretions from horses infected with A/equine influenza (H3N8) viruses.
J Virol Methods, 20(1), 1-12.
https://doi.org/10.1016/0166-0934(88)90034-1 Publication
Researcher Affiliations
- Equine Virology Unit, Animal Health Trust, Newmarket, Suffolk, U.K.
MeSH Terms
- Animals
- Antibodies, Monoclonal
- Antigens, Viral / analysis
- Antigens, Viral / immunology
- Cell Line
- Enzyme-Linked Immunosorbent Assay
- Horse Diseases / diagnosis
- Horse Diseases / immunology
- Horses
- Influenza A Virus, H3N8 Subtype
- Influenza A virus / immunology
- Influenza A virus / isolation & purification
- Nasal Mucosa / immunology
- Nasal Mucosa / metabolism
- Nucleoproteins / analysis
- Nucleoproteins / immunology
- Orthomyxoviridae Infections / diagnosis
- Orthomyxoviridae Infections / immunology
- Orthomyxoviridae Infections / veterinary
Citations
This article has been cited 6 times.- Singh RK, Dhama K, Karthik K, Khandia R, Munjal A, Khurana SK, Chakraborty S, Malik YS, Virmani N, Singh R, Tripathi BN, Munir M, van der Kolk JH. A Comprehensive Review on Equine Influenza Virus: Etiology, Epidemiology, Pathobiology, Advances in Developing Diagnostics, Vaccines, and Control Strategies.. Front Microbiol 2018;9:1941.
- Rash A, Morton R, Woodward A, Maes O, McCauley J, Bryant N, Elton D. Evolution and Divergence of H3N8 Equine Influenza Viruses Circulating in the United Kingdom from 2013 to 2015.. Pathogens 2017 Feb 8;6(1).
- Murcia PR, Baillie GJ, Stack JC, Jervis C, Elton D, Mumford JA, Daly J, Kellam P, Grenfell BT, Holmes EC, Wood JL. Evolution of equine influenza virus in vaccinated horses.. J Virol 2013 Apr;87(8):4768-71.
- Murcia PR, Baillie GJ, Daly J, Elton D, Jervis C, Mumford JA, Newton R, Parrish CR, Hoelzer K, Dougan G, Parkhill J, Lennard N, Ormond D, Moule S, Whitwham A, McCauley JW, McKinley TJ, Holmes EC, Grenfell BT, Wood JL. Intra- and interhost evolutionary dynamics of equine influenza virus.. J Virol 2010 Jul;84(14):6943-54.
- Bryant NA, Paillot R, Rash AS, Medcalf E, Montesso F, Ross J, Watson J, Jeggo M, Lewis NS, Newton JR, Elton DM. Comparison of two modern vaccines and previous influenza infection against challenge with an equine influenza virus from the Australian 2007 outbreak.. Vet Res 2010 Mar-Apr;41(2):19.
- Sinclair R, Moult BJ, Mumford JA. Characterization of an antigenic site on glycoprotein 13 (gC) of equid herpesvirus type-1.. Arch Virol 1993;129(1-4):327-36.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
