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Home / Equine Research Bank / J Virol / Detection of latency-associated transcripts of equid herpesv...
Journal of virology1997; 71(5); 3437-3443; doi: 10.1128/JVI.71.5.3437-3443.1997

Detection of latency-associated transcripts of equid herpesvirus 1 in equine leukocytes but not in trigeminal ganglia.

Abstract: Results from Southern hybridization and PCR amplification experiments using a randomly synthesized reverse transcription-PCR product showed that peripheral blood leukocytes from horses showing no clinical signs of disease expressed a putative latency-associated transcript antisense to and overlapping the 3' end of the equid herpesvirus 1 (EHV-1) immediate-early gene (gene 64). A PCR product derived from this transcript has > or =96% identity with the published EHV-1 sequence. In situ hybridization studies of equine bronchial lymph nodes corroborated these findings and are consistent with reactivation data (D. A. Smith, A. Hamblin, and N. Edington, unpublished data), indicating that EHV-1 latency is established predominantly in CD5+/CD8+ leukocytes.
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Publication Date: 1997-05-01 PubMed ID: 9094614PubMed Central: PMC191489DOI: 10.1128/JVI.71.5.3437-3443.1997Google Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study explored and confirmed the presence of equid herpesvirus 1 (EHV-1) transcripts in horse leukocytes, but not in their trigeminal ganglia. The experiment aided understanding of how EHV-1 establishes itself in CD5+/CD8+ leukocytes, leading to viral latency.

Detection of EHV-1 Transcripts

  • The research began with Southern hybridization and PCR (Polymerase Chain Reaction) experiments, which used products obtained from reverse transcription-PCR.
  • The cells investigated were peripheral blood leukocytes extracted from healthy horses without any visible clinical signs of disease.
  • These experiments successfully led to the identification of a probable latency-associated transcript on the reverse side and overlapping the 3′ end of the EHV-1 immediate-early gene (gene 64).

Comparison with Published EHV-1 Sequence

  • A PCR product emerging from this transcript showed a high similarity, equal to or exceeding 96% identity, to the published EHV-1 sequence.
  • This high level of correlation confirmed the presence of the EHV-1 transcript in the tested leukocytes.

In situ Hybridization and EHV-1 Latency

  • The researchers also conducted in situ hybridization studies focusing on equine bronchial lymph nodes, which supported the initial findings.
  • Data from these studies was in agreement with reactivation data from other unpublished research, suggesting that EHV-1 latency predominantly occurs within CD5+/CD8+ leukocytes.
  • In other words, EHV-1 was found to largely establish its latency or inactive state in a specific category of immune cells, presenting new insights into EHV-1’s survival strategies in its host.

Further Considerations and Implications

  • The fact that EHV-1 latency-associated transcripts weren’t detected in the trigeminal ganglia of the horses carries implications for the study and understanding of neurotropic herpesviruses in general.
  • This research thus contributes to the existing body of knowledge about equid herpesvirus 1 and its latency in horses, which could potentially be used to develop more effective treatments and containment strategies for this virus.

Cite This Article

APA
Chesters PM, Allsop R, Purewal A, Edington N. (1997). Detection of latency-associated transcripts of equid herpesvirus 1 in equine leukocytes but not in trigeminal ganglia. J Virol, 71(5), 3437-3443. https://doi.org/10.1128/JVI.71.5.3437-3443.1997

Publication

Journal of virology
ISSN: 0022-538X
NlmUniqueID: 0113724
Country: United States
Language: English
Volume: 71
Issue: 5
Pages: 3437-3443

Researcher Affiliations

Chesters, P M
  • Department of Pathology and Infectious Diseases, The Royal Veterinary College, London, United Kingdom.
Allsop, R
    Purewal, A
      Edington, N

        MeSH Terms

        • Animals
        • Blotting, Southern
        • Herpesvirus 1, Equid / genetics
        • Herpesvirus 1, Equid / physiology
        • Horses
        • In Situ Hybridization
        • Leukocytes / virology
        • Polymerase Chain Reaction
        • RNA, Messenger / analysis
        • Trigeminal Ganglion / virology
        • Virus Latency

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