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Home / Equine Research Bank / Emerg Infect Dis / Detection of North American West Nile virus in animal tissue...
Emerging infectious diseases2001; 7(4); 739-741; doi: 10.3201/eid0704.010425

Detection of North American West Nile virus in animal tissue by a reverse transcription-nested polymerase chain reaction assay.

Abstract: A traditional single-stage reverse transcription-polymerase chain reaction (RT-PCR) procedure is effective in determining West Nile (WN) virus in avian tissue and infected cell cultures. However, the procedure lacks the sensitivity to detect WN virus in equine tissue. We describe an RT-nested PCR (RT-nPCR) procedure that identifies the North American strain of WN virus directly in equine and avian tissues.
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Publication Date: 2001-10-05 PubMed ID: 11585541PubMed Central: PMC2631755DOI: 10.3201/eid0704.010425Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article explains a method that determines the presence of the North American strain of West Nile virus in equine and avian tissues, using reverse transcription-nested polymerase chain reaction (RT-nPCR).

Introduction

  • The study focused on developing a more effective testing procedure for determining the presence of West Nile (WN) virus in animal tissues, particularly in horses (equine tissue).
  • The need for a new method arose because the traditional single-stage reverse transcription-polymerase chain reaction (RT-PCR) procedure, while effective in detecting the virus in avian tissues and infected cell cultures, lacked the sensitivity needed for equine tissue.

Reverse Transcription-Nested Polymerase Chain Reaction (RT-nPCR) Technique

  • The paper introduces a new process, called RT-nested PCR (RT-nPCR), which is capable of identifying the North American strain of WN virus directly in both equine and avian tissues.
  • RT-nPCR is a molecular technique often used for the detection of very low copy number RNA and DNA, due to its high sensitivity and specificity.
  • The process involves two rounds of PCR. The second round is specifically designed to improve the sensitivity of the test, which is the main advantage of this procedure over RT-PCR.

Implications and Significance of the Study

  • The successful use of RT-nPCR in this study could pave the way for improved detection of WN virus in animal tissues, benefiting veterinary diagnostic tools and procedures.
  • By accurately identifying the presence of this virus, appropriate actions can be taken promptly to prevent the spread of disease and improve animal health.
  • This research may also open doors for the development of similar testing procedures for other types of viruses, particularly those that are difficult to detect in certain types of tissue using common testing methods.

Cite This Article

APA
Johnson DJ, Ostlund EN, Pedersen DD, Schmitt BJ. (2001). Detection of North American West Nile virus in animal tissue by a reverse transcription-nested polymerase chain reaction assay. Emerg Infect Dis, 7(4), 739-741. https://doi.org/10.3201/eid0704.010425

Publication

Emerging infectious diseases
ISSN: 1080-6040
NlmUniqueID: 9508155
Country: United States
Language: English
Volume: 7
Issue: 4
Pages: 739-741

Researcher Affiliations

Johnson, D J
  • Animal and Plant Health Inspection Service, U.S. Department of Agriculture, 1800 Dayton Ave., Ames, IA 50010-0844, USA. donna.j.johnson@aphis.usda.gov
Ostlund, E N
    Pedersen, D D
      Schmitt, B J

        MeSH Terms

        • Animals
        • Bird Diseases / epidemiology
        • Bird Diseases / pathology
        • Bird Diseases / virology
        • Birds / virology
        • Brain / pathology
        • Brain / virology
        • Disease Reservoirs / veterinary
        • Horse Diseases / epidemiology
        • Horse Diseases / pathology
        • Horse Diseases / virology
        • Horses / virology
        • New York / epidemiology
        • North America
        • RNA, Viral / analysis
        • Reverse Transcriptase Polymerase Chain Reaction
        • Sensitivity and Specificity
        • West Nile Fever / epidemiology
        • West Nile Fever / pathology
        • West Nile Fever / veterinary
        • West Nile Fever / virology
        • West Nile virus / genetics
        • West Nile virus / isolation & purification

        References

        This article includes 4 references
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        This article has been cited 17 times.
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