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PLoS neglected tropical diseases2014; 8(7); e3028; doi: 10.1371/journal.pntd.0003028

Serological, molecular and entomological surveillance demonstrates widespread circulation of West Nile virus in Turkey.

Abstract: West Nile virus (WNV), a mosquito-borne flavivirus with significant impact on human and animal health, has recently demonstrated an expanded zone of activity globally. The aim of this study is to investigate the frequency and distribution of WNV infections in potential vectors and several mammal and avian species in Turkey, where previous data indicate viral circulation. The study was conducted in 15 provinces across Turkey during 2011-2013. In addition, the entomological study was extended to 4 districts of the Turkish Republic of Northern Cyprus. WNV exposure was determined in humans, horses, sheep and ducks from Mersin, Sanliurfa, Van and Kars provinces of Turkey, via the detection of neutralizing antibodies. WNV RNA was sought in human and equine samples from Mersin, Adana and Mugla provinces. Field-collected mosquitoes from 92 sites at 46 locations were characterized morphologically and evaluated for viral RNA. Neutralizing antibodies were identified in 10.5% of the 1180 samples studied and detected in all species evaluated. Viral nucleic acids were observed in 5.9% of 522 samples but only in horses. A total of 2642 mosquito specimens belonging to 15 species were captured, where Ochlerotatus caspius (52.4%), Culex pipiens sensu lato (24.2%) comprise the most frequent species. WNV RNA was detected in 4 mosquito pools (1.9%), that comprise Oc. caspius Cx. pipiens s.l. and DNA barcoding revealed the presence of Cx. quinquefasciatus and Cx. perexiguus mosquitoes in infected Culex pools. All WNV partial sequences were characterized as lineage 1 clade 1a. These findings indicate a widespread WNV activity in Turkey, in Eastern Thrace and Mediterranean-Aegean regions as well as Southeastern and Northeastern Anatolia.
Publication Date: 2014-07-24 PubMed ID: 25058465PubMed Central: PMC4109882DOI: 10.1371/journal.pntd.0003028Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article is about the widespread occurrence of the West Nile Virus (WNV), a disease mainly transmitted by mosquitoes, in Turkey; it was detected in multiple types of animals and human beings in various parts of the country.

Research Overview

  • The objective of this study was to determine the prevalence and spread of West Nile Virus (WNV) infections in potential vectors (organisms that transmit diseases), and several mammal and bird species in Turkey. The study was conducted because previous data indicated the presence of the virus in Turkey.
  • The research was carried out in 15 provinces throughout Turkey over a course of two years (2011 – 2013), with extension into Northern Cyprus. The geographical diversity covered in the study provides a comprehensive overview of the WNV situation in the country.

Research Methodology

  • Researchers collected samples from humans, horses, sheep, and ducks. The presence of WNV was determined by looking for detection of neutralizing antibodies (type of proteins the immune system uses to neutralize harmful pathogens) in these samples.
  • WNV RNA was also sought in human and equine (horse) samples. This was to determine if there was a replicating viral infection present in these samples.
  • Field-collected mosquitoes from 92 sites were characterized based on their morphological features and evaluated for any presence of viral RNA.

Findings

  • Among the 1180 samples studied, 10.5% had neutralizing antibodies, indicating previous exposure to WNV in all sampled species. This suggested that the virus was indeed circulating in the wildlife in the region.
  • Viral nucleic acids were found in 5.9% of 522 samples, but only in horses. This may mean horses could be potential higher risk hosts for the virus to replicate.
  • Among the 2642 mosquito samples collected, the species Ochlerotatus caspius and Culex pipiens sensu lato were identified to carry the most WNV RNA. DNA barcoding confirmed the presence of other mosquito species in the infected pools.
  • All WNV sequences were identified as part of lineage 1 clade 1a, a classification denoting a specific genetic strain or type of WNV.

Conclusion

  • The study confirmed a widespread activity of WNV across Turkey, from the Mediterranean-Aegean regions to Southeastern and Northeastern Anatolia.
  • This finding helps in understanding the epidemiology of WNV in the region, making it possible to develop targeted measures for disease prevention.

Cite This Article

APA
Ergunay K, Gunay F, Erisoz Kasap O, Oter K, Gargari S, Karaoglu T, Tezcan S, Cabalar M, Yildirim Y, Emekdas G, Alten B, Ozkul A. (2014). Serological, molecular and entomological surveillance demonstrates widespread circulation of West Nile virus in Turkey. PLoS Negl Trop Dis, 8(7), e3028. https://doi.org/10.1371/journal.pntd.0003028

Publication

ISSN: 1935-2735
NlmUniqueID: 101291488
Country: United States
Language: English
Volume: 8
Issue: 7
Pages: e3028

Researcher Affiliations

Ergunay, Koray
  • Faculty of Medicine, Department of Medical Microbiology, Virology Unit, Hacettepe University, Ankara, Turkey.
Gunay, Filiz
  • Faculty of Sciences, Department of Biology, Division of Ecology, Hacettepe University, Ankara, Turkey.
Erisoz Kasap, Ozge
  • Faculty of Sciences, Department of Biology, Division of Ecology, Hacettepe University, Ankara, Turkey.
Oter, Kerem
  • Faculty of Veterinary Medicine, Department of Parasitology, Istanbul University, Istanbul, Turkey.
Gargari, Sepandar
  • Faculty of Veterinary Medicine, Department of Virology, Ankara University, Ankara, Turkey.
Karaoglu, Taner
  • Faculty of Veterinary Medicine, Department of Virology, Ankara University, Ankara, Turkey.
Tezcan, Seda
  • Faculty of Medicine, Department of Medical Microbiology, Mersin University, Mersin, Turkey.
Cabalar, Mehmet
  • Faculty of Veterinary Medicine, Department of Virology, Harran University, Ankara, Turkey.
Yildirim, Yakup
  • Faculty of Veterinary Medicine, Department of Virology, Kafkas University, Ankara, Turkey.
Emekdas, Gürol
  • Faculty of Medicine, Department of Medical Microbiology, Mersin University, Mersin, Turkey.
Alten, Bulent
  • Faculty of Sciences, Department of Biology, Division of Ecology, Hacettepe University, Ankara, Turkey.
Ozkul, Aykut
  • Faculty of Veterinary Medicine, Department of Virology, Ankara University, Ankara, Turkey.

MeSH Terms

  • Animals
  • Birds / virology
  • Culicidae / virology
  • Horses / virology
  • Humans
  • Molecular Sequence Data
  • Public Health Surveillance
  • Turkey / epidemiology
  • West Nile Fever / epidemiology
  • West Nile Fever / veterinary
  • West Nile Fever / virology
  • West Nile virus

Conflict of Interest Statement

The authors have declared that no competing interests exist.

References

This article includes 47 references
  1. Sambri V, Capobianchi M, Charrel R, Fyodorova M, Gaibani P. West Nile virus in Europe: emergence, epidemiology, diagnosis, treatment, and prevention.. Clin Microbiol Infect 19: 699–704.
    pubmed: 23594175
  2. Dauphin G, Zientara S, Zeller H, Murgue B. West Nile: worldwide current situation in animals and humans.. Comp Immunol Microbiol Infect Dis 27: 343–355.
    pubmed: 15225984
  3. Calistri P, Giovannini A, Hubalek Z, Ionescu A. Epidemiology of West Nile in Europe and in the Mediterranean Basin.. Open Virol J 4: 29–37.
    pmc: PMC2878979pubmed: 20517490
  4. Kilpatrick AM. Globalization, land use, and the invasion of West Nile virus.. Science 334: 323–327.
    pmc: PMC3346291pubmed: 22021850
  5. Beasley DW, Barrett AD, Tesh RB. Resurgence of West Nile neurologic disease in the United States in 2012: what happened? What needs to be done?. Antiviral Res 99: 1–5.
    pubmed: 23624155
  6. Lanciotti RS, Roehrig JT, Deubel V, Smith J, Parker M. Origin of the West Nile virus responsible for an outbreak of encephalitis in the northeastern United States.. Science 286: 2333–2337.
    pubmed: 10600742
  7. Komar N, Clark GG. West Nile virus activity in Latin America and the Caribbean.. Rev Panam Salud Publica 19: 112–117.
    pubmed: 16551385
  8. Monath TP. Flaviviruses.. In: Fields BN, Knipe M, editors, Virology, 2nd Edn, Raven Press, New York. pp.763–814.
  9. Hayes EB, Sejvar JJ, Zaki SR, Lanciotti RS, Bode AV. Virology, pathology and clinical manifestations of West Nile Virus disease.. Emerg Infect Dis 11: 1174–1179.
    pmc: PMC3320472pubmed: 16102303
  10. Chambers TJ, Hahn CS, Galler R, Rice CM. Flavivirus genome organization, expression, and replication.. Annu Rev Microbiol 44: 649–688.
    pubmed: 2174669
  11. May FJ, Davis CT, Tesh RB, Barrett AD. Phylogeography of West Nile virus.. J Virol 85: 2964–2974.
    pmc: PMC3067944pubmed: 21159871
  12. Hayes EB, Komar N, Nasci RS, Montgomery SP, O'Leary DR. Epidemiology and transmission dynamics of West Nile virus disease.. Emerg Infect Dis 11: 1167–1173.
    pmc: PMC3320478pubmed: 16102302
  13. Colpitts TM, Conway MJ, Montgomery RR, Fikrig E. West Nile Virus: biology, transmission, and human infection.. Clin Microbiol Rev 25: 635–648.
    pmc: PMC3485754pubmed: 23034323
  14. Ergunay K, Whitehouse C, Ozkul A. Current status of human arboviral infections in Turkey.. Vector Borne Zoonotic Dis 11: 731–741.
    pubmed: 21133776
  15. Kalaycioglu H, Korukluoglu G, Ozkul A, Oncul O, Tosun S. Emergence of West Nile virus infections in humans in Turkey, 2010 to 2011.. Euro Surveill 17: pii: 20182.
    pubmed: 22687827
  16. Ozkul A, Ergunay K, Koysuren A, Alkan F, Arsava EM. Concurrent occurrence of human and equine West Nile virus infections in Central Anatolia, Turkey: the first evidence for circulation of lineage 1 viruses.. Int J Infect Dis 17: 546–551.
    pubmed: 23517780
  17. Erdem H, Ergunay K, Yilmaz A, Naz H, Akata F. Emergence and co-infections of West Nile virus and Toscana virus in Eastern Thrace, Turkey.. Clin Microbiol Infect 20: 319–325.
    pubmed: 23910388
  18. Ergunay K, Gunay F, Oter K, Kasap OE, Orsten S. Arboviral surveillance of field-collected mosquitoes reveals circulation of West Nile virus lineage 1 strains in Eastern Thrace, Turkey.. Vector Borne Zoonotic Dis 744–752.
    pubmed: 23919608
  19. Bunning ML, Bowen RA, Cropp CB, Sullivan KG, Davis B. Experimental infection of horses with West Nile virus.. Emerg Infect Dis 8: 380–386.
    pmc: PMC3393377pubmed: 11971771
  20. Darsie RE, Samanidou-Voyadjoglou A. Keys for the identification of the mosquitoes of Greece.. J Am Mosq Control Assoc 13: 247–254.
    pubmed: 9383766
  21. Schaffner E, Angel G, Geoffroy B, Hervy JP, Rhaiem A. The Mosquitoes of Europe.. [CD-ROM] Paris: IRD Éditions & EID Méditerrannée.
  22. Calzolari M, Bonilauri P, Bellini R, Caimi M, Defilippo F. Arboviral survey of mosquitoes in two northern Italian regions in 2007 and 2008.. Vector Borne Zoonotic Dis 10: 875–884.
    pubmed: 20370434
  23. Johnson DJ, Ostlund EN, Pedersen DD, Schmitt BJ. Detection of North American West Nile virus in animal tissue by a reverse transcription-nested polymerase chain reaction assay.. Emerg Infect Dis 7: 739–741.
    pmc: PMC2631755pubmed: 11585541
  24. Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates.. Mol Mar Biol Biotechnol 3: 294–299.
    pubmed: 7881515
  25. Tamura K, Peterson D, Peterson N, Stecher G, Nei M. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance and maximum parsimony methods.. Mol Biol Evol 28: 2731–2739.
    pmc: PMC3203626pubmed: 21546353
  26. Ozkul A, Yildirim Y, Pinar D, Akcali A, Yilmaz V. Serological evidence of West Nile Virus (WNV) in mammalian species in Turkey.. Epidemiol Infect 134: 826–829.
    pmc: PMC2870448pubmed: 16316496
  27. Ergunay K, Ozer N, Us D, Ozkul A, Simsek F. Seroprevalence of West Nile virus and tick-borne encephalitis virus in southeastern Turkey: first evidence for tick-borne encephalitis virus infections.. Vector Borne Zoonotic Dis 7: 157–161.
    pubmed: 17627432
  28. Karakoc ZC, Tüzüner BM, Ergonul O, Pierro A, Di Fonzo E. West nile virus infection in the Mesopotamia region, Syria border of Turkey.. Vector Borne Zoonotic Dis 13: 739–743.
    pubmed: 23808974
  29. Angenvoort J, Brault AC, Bowen RA, Groschup MH. West Nile viral infection of equids.. Vet Microbiol 167: 168–180.
    pmc: PMC4581842pubmed: 24035480
  30. Gardner IA, Wong SJ, Ferraro GL, Balasuriya UB, Hullinger PJ. Incidence and effects of West Nile virus infection in vaccinated and unvaccinated horses in California.. Vet Res 38: 109–116.
    pubmed: 17274156
  31. Nielsen CF, Reisen WK, Armijos MV, Maclachlan NJ, Scott TW. High subclinical West Nile virus incidence among nonvaccinated horses in northern California associated with low vector abundance and infection.. Am J Trop Med Hyg 78: 45–52.
    pubmed: 18187784
  32. Garcia-Bocanegra I, Jaen-Tellez JA, Napp S, Arenas-Montes A, Fernandez-Morente M. West Nile fever outbreak in horses and humans, Spain, 2010.. Emerg Infect Dis 17: 2397–2399.
    pmc: PMC3311180pubmed: 22172565
  33. Albayrak H, Ozan E. Molecular detection of avian influenza virus but not West Nile virus in wild birds in northern Turkey.. Zoonoses Public Health 57: 71–75.
    pubmed: 20298488
  34. Seino KK, Long MT, Gibbs EP, Bowen RA, Beachboard SE. Comparative efficacies of three commercially available vaccines against West Nile Virus (WNV) in a short-duration challenge trial involving an equine WNV encephalitis model.. Clin Vaccine Immunol 14: 1465–1471.
    pmc: PMC2168174pubmed: 17687109
  35. Minke JM, Siger L, Cupillard L, Powers B, Bakonyi T. Protection provided by a recombinant ALVAC(R)-WNV vaccine expressing the prM/E genes of a lineage 1 strain of WNV against a virulent challenge with a lineage 2 strain.. Vaccine 29: 4608–4612.
    pubmed: 21549780
  36. Sardelis MR, Turrell MJ, Dohm DJ, O'Guinn ML. Vector competence of selected North American Culex and Coquillettidia mosquitoes for West Nile virus.. Emerg Infect Dis 7: 1018–1022.
    pmc: PMC2631924pubmed: 11747732
  37. Balenghien T, Vazeille M, Grandadam M, Schaffner F, Zeller H. Vector competence of some French Culex and Aedes mosquitoes for West Nile virus.. Vector Borne Zoonotic Dis 8: 589–595.
    pubmed: 18447623
  38. Engler O, Savini G, Papa A, Figuerola J, Groschup MH. European surveillance for West Nile virus in mosquito populations.. Int J Environ Res Public Health 10: 4869–4895.
    pmc: PMC3823308pubmed: 24157510
  39. Ramsdale CD, Alten B, Caglar SS, Ozer N. A revised, annotated checklist of the mosquitoes (Diptera, Culicidae) of Turkey.. Eur Mosq Bull 9: 18–28.
  40. Fall G, Diallo M, Loucoubar C, Faye O, Sall AA. Vector competence of Culex neavei and Culex quinquefasciatus (Diptera: Culicidae) from Senegal for lineages 1, 2, Koutango and a putative new lineage of West Nile virus.. Am J Trop Med Hyg .
    doi: 10.4269/ajtmh.13-0405pmc: PMC3973524pubmed: 24567319google scholar: lookup
  41. Munoz J1, Ruiz S, Soriguer R, Alcaide M, Viana DS. Feeding patterns of potential West Nile virus vectors in south-west Spain.. PLoS One 7: e39549.
    pmc: PMC3382169pubmed: 22745781
  42. Ozer N, Ergunay K, Simsek F, Kaynas S, Alten B. West Nile virus studies in the Sanliurfa Province of Turkey.. J Vector Ecol 32: 202–206.
    pubmed: 18260509
  43. Ocal M, Orsten S, Inkaya AC, Yetim E, Acar NP. Ongoing activity of Toscana virus genotype A and West Nile virus lineage 1 strains in Turkey: a clinical and field survey.. Zoonoses Public Health .
    doi: 10.1111/zph.12096pubmed: 25285941google scholar: lookup
  44. Fyodorova MV, Savage HM, Lopatina JV, Bulgakova TA, Ivanitsky AV. Evaluation of potential West Nile virus vectors in Volgograd region, 2003 (Diptera: Culicidae): Species composition, bloodmeal host utilization, and virus infection rates of mosquitoes.. J Med Entomol 43: 552–563.
    pubmed: 16739415
  45. Orshan L, Bin H, Schnur H, Kaufman A, Valinsky A. Mosquito vectors of West Nile fever in Israel.. J Med Entomol 45: 939–947.
    pubmed: 18826039
  46. Bakonyi T, Ivanics E, Erdelyi K, Ursu K, Ferenczi E. Lineage 1 and 2 strains of encephalitic West Nile virus, central Europe.. Emerg Infect Dis 12: 618–623.
    pmc: PMC3294705pubmed: 16704810
  47. Bakonyi T, Ferenczi E, Erdelyi K, Kutasi O, Csorgo T. Explosive spread of a neuroinvasive lineage 2 West Nile virus in Central Europe, 2008/2009.. Vet Microbiol 165: 61–70.
    pubmed: 23570864

Citations

This article has been cited 33 times.
  1. Barhoumi W, Khedhiri M, M'Ghirbi Y, Bougatef S, Touzi H, Rhim A, Karray H, Trabelsi A, Mastouri M, Hannachi N, Bouattour A, Triki H, Ben Alaya NB, Fares W. Surveillance of West Nile Virus in Tunisia: Evidence from Human and Entomological Investigation. Viruses 2025 Nov 29;17(12).
    doi: 10.3390/v17121562pubmed: 41472233google scholar: lookup
  2. Aytekin S, Sakaci Z, Talay S, Alten B. Effects of High Larval Density on Wing Shape Deformations of Culex pipiens (Culicidae: Diptera) via Geometric Morphometrics. Insects 2025 Nov 21;16(12).
    doi: 10.3390/insects16121185pubmed: 41465625google scholar: lookup
  3. Volkan E, Karanis P. Current Risks and Prevention Strategies Against Vector-Borne Diseases in Cyprus. Microorganisms 2025 Mar 24;13(4).
  4. Sewade W, Polat C, Kasap OE. Molecular evidence of Wolbachia and Orthoflavivirus infection in field-collected mosquitoes in three provinces of Türkiye. Trop Med Int Health 2025 Jun;30(6):498-508.
    doi: 10.1111/tmi.14099pubmed: 40229230google scholar: lookup
  5. Özyörük F, Özgünlük İ, Turgut Sİ, Şengül Y, Yığman AA, Gülyaz V. Environmental and Breed-Related Determinants of Bovine Ephemeral Fever Outbreaks in Southeastern Türkiye. Vet Med Sci 2025 Mar;11(2):e70257.
    doi: 10.1002/vms3.70257pubmed: 40065586google scholar: lookup
  6. Rusenova N, Rusenov A, Chervenkov M, Sirakov I. Seroprevalence of West Nile Virus among Equids in Bulgaria in 2022 and Assessment of Some Risk Factors. Vet Sci 2024 May 9;11(5).
    doi: 10.3390/vetsci11050209pubmed: 38787181google scholar: lookup
  7. Bursali F, Simsek FM. Population Genetics of Culex tritaeniorhynchus (Diptera: Culicidae) in Türkiye. Acta Parasitol 2024 Jun;69(2):1157-1171.
    doi: 10.1007/s11686-024-00844-9pubmed: 38592372google scholar: lookup
  8. Bektore B, Dogan B, Ozkul A, Gozalan A. West Nile virus seropositivity in Alanya, a coastal city in the Mediterranean region of Turkey. Ann Saudi Med 2024 Jan-Feb;44(1):48-54.
    doi: 10.5144/0256-4947.2024.48pubmed: 38311862google scholar: lookup
  9. Natasha JA, Yasmin AR, Sharma RSK, Nur-Fazila SH, Nur-Mahiza MI, Arshad SS, Mohammed HO, Kumar K, Loong SK, Ahmad Khusaini MKS. Circulation of West Nile virus in mosquitoes approximate to the migratory bird stopover in West Coast Malaysia. PLoS Negl Trop Dis 2023 Apr;17(4):e0011255.
    doi: 10.1371/journal.pntd.0011255pubmed: 37023172google scholar: lookup
  10. Hoque MM, Valentine MJ, Kelly PJ, Barua S, Murillo DFB, Wang C. Modification of the Folmer primers for the cytochrome c oxidase gene facilitates identification of mosquitoes. Parasit Vectors 2022 Nov 22;15(1):437.
    doi: 10.1186/s13071-022-05494-2pubmed: 36419198google scholar: lookup
  11. Zhang YM, Guo XX, Jiang SF, Li CX, Xing D, Zhang HD, Dong YD, Zhao TY. The Potential Vector Competence and Overwintering of West Nile Virus in Vector Aedes Albopictus in China. Front Microbiol 2022;13:888751.
    doi: 10.3389/fmicb.2022.888751pubmed: 35722287google scholar: lookup
  12. Bertola M, Mazzucato M, Pombi M, Montarsi F. Updated occurrence and bionomics of potential malaria vectors in Europe: a systematic review (2000-2021). Parasit Vectors 2022 Mar 15;15(1):88.
    doi: 10.1186/s13071-022-05204-ypubmed: 35292106google scholar: lookup
  13. Yıldırım Y, Yılmaz V, Yazıcı K, Öziç C, Ozkul A, Çağırgan AA. Phylogenetic analysis of West Nile virus: first report of lineage 1 in donkey in Turkey. Trop Anim Health Prod 2021 Sep 18;53(5):453.
    doi: 10.1007/s11250-021-02892-zpubmed: 34536145google scholar: lookup
  14. Zhang Y, Lei W, Wang Y, Sui H, Liu B, Li F, He Y, Li Z, Fu S, Wang L, Xu L, Mahe M, Gao Z, Mamutijiang T, Lv Z, Xiang N, Zhou L, Ni D, Liang G, Li Q, Wang H, Feng Z. Surveillance of West Nile virus infection in Kashgar Region, Xinjiang, China, 2013-2016. Sci Rep 2021 Jul 7;11(1):14010.
    doi: 10.1038/s41598-021-93309-2pubmed: 34234184google scholar: lookup
  15. Abdelkrim O, Samia B, Said Z, Souad L. Modeling and mapping the habitat suitability and the potential distribution of Arboviruses vectors in Morocco. Parasite 2021;28:37.
    doi: 10.1051/parasite/2021030pubmed: 33861197google scholar: lookup
  16. Saiz JC, Martín-Acebes MA, Blázquez AB, Escribano-Romero E, Poderoso T, Jiménez de Oya N. Pathogenicity and virulence of West Nile virus revisited eight decades after its first isolation. Virulence 2021 Dec;12(1):1145-1173.
    doi: 10.1080/21505594.2021.1908740pubmed: 33843445google scholar: lookup
  17. Bakhshi H, Mousson L, Vazeille M, Zakeri S, Raz A, de Lamballerie X, Dinparast-Djadid N, Failloux AB. High Transmission Potential of West Nile Virus Lineage 1 for Cx. pipiens s.l. of Iran. Viruses 2020 Apr 3;12(4).
    doi: 10.3390/v12040397pubmed: 32260215google scholar: lookup
  18. Chevalier V, Marsot M, Molia S, Rasamoelina H, Rakotondravao R, Pedrono M, Lowenski S, Durand B, Lecollinet S, Beck C. Serological Evidence of West Nile and Usutu Viruses Circulation in Domestic and Wild Birds in Wetlands of Mali and Madagascar in 2008. Int J Environ Res Public Health 2020 Mar 18;17(6).
    doi: 10.3390/ijerph17061998pubmed: 32197367google scholar: lookup
  19. Barba M, Fairbanks EL, Daly JM. Equine viral encephalitis: prevalence, impact, and management strategies. Vet Med (Auckl) 2019;10:99-110.
    doi: 10.2147/VMRR.S168227pubmed: 31497528google scholar: lookup
  20. Billioud G, Tryfonos C, Richter J. The Prevalence of Antibodies against Sandfly Fever Viruses and West Nile Virus in Cyprus. J Arthropod Borne Dis 2019 Mar;13(1):116-125.
    pubmed: 31346541
  21. Jourdain F, Samy AM, Hamidi A, Bouattour A, Alten B, Faraj C, Roiz D, Petrić D, Pérez-Ramírez E, Velo E, Günay F, Bosevska G, Salem I, Pajovic I, Marić J, Kanani K, Paronyan L, Dente MG, Picard M, Zgomba M, Sarih M, Haddad N, Gaidash O, Sukhiasvili R, Declich S, Shaibi T, Sulesco T, Harrat Z, Robert V. Towards harmonisation of entomological surveillance in the Mediterranean area. PLoS Negl Trop Dis 2019 Jun;13(6):e0007314.
    doi: 10.1371/journal.pntd.0007314pubmed: 31194743google scholar: lookup
  22. Akıner MM, Öztürk M, Başer AB, Günay F, Hacıoğlu S, Brinkmann A, Emanet N, Alten B, Özkul A, Nitsche A, Linton YM, Ergünay K. Arboviral screening of invasive Aedes species in northeastern Turkey: West Nile virus circulation and detection of insect-only viruses. PLoS Negl Trop Dis 2019 May;13(5):e0007334.
    doi: 10.1371/journal.pntd.0007334pubmed: 31059502google scholar: lookup
  23. Ziyaeyan M, Behzadi MA, Leyva-Grado VH, Azizi K, Pouladfar G, Dorzaban H, Ziyaeyan A, Salek S, Jaber Hashemi A, Jamalidoust M. Widespread circulation of West Nile virus, but not Zika virus in southern Iran. PLoS Negl Trop Dis 2018 Dec;12(12):e0007022.
    doi: 10.1371/journal.pntd.0007022pubmed: 30557321google scholar: lookup
  24. Lustig Y, Sofer D, Bucris ED, Mendelson E. Surveillance and Diagnosis of West Nile Virus in the Face of Flavivirus Cross-Reactivity. Front Microbiol 2018;9:2421.
    doi: 10.3389/fmicb.2018.02421pubmed: 30369916google scholar: lookup
  25. Kumar B, Manuja A, Gulati BR, Virmani N, Tripathi BN. Zoonotic Viral Diseases of Equines and Their Impact on Human and Animal Health. Open Virol J 2018;12:80-98.
    doi: 10.2174/1874357901812010080pubmed: 30288197google scholar: lookup
  26. Altinli M, Gunay F, Alten B, Weill M, Sicard M. Wolbachia diversity and cytoplasmic incompatibility patterns in Culex pipiens populations in Turkey. Parasit Vectors 2018 Mar 20;11(1):198.
    doi: 10.1186/s13071-018-2777-9pubmed: 29558974google scholar: lookup
  27. Martínez-de la Puente J, Ferraguti M, Ruiz S, Roiz D, Llorente F, Pérez-Ramírez E, Jiménez-Clavero MÁ, Soriguer R, Figuerola J. Mosquito community influences West Nile virus seroprevalence in wild birds: implications for the risk of spillover into human populations. Sci Rep 2018 Feb 8;8(1):2599.
    doi: 10.1038/s41598-018-20825-zpubmed: 29422507google scholar: lookup
  28. Failloux AB, Bouattour A, Faraj C, Gunay F, Haddad N, Harrat Z, Jancheska E, Kanani K, Kenawy MA, Kota M, Pajovic I, Paronyan L, Petric D, Sarih M, Sawalha S, Shaibi T, Sherifi K, Sulesco T, Velo E, Gaayeb L, Victoir K, Robert V. Surveillance of Arthropod-Borne Viruses and Their Vectors in the Mediterranean and Black Sea Regions Within the MediLabSecure Network. Curr Trop Med Rep 2017;4(1):27-39.
    doi: 10.1007/s40475-017-0101-ypubmed: 28386524google scholar: lookup
  29. Ergünay K, Litzba N, Brinkmann A, Günay F, Sarıkaya Y, Kar S, Örsten S, Öter K, Domingo C, Erisoz Kasap Ö, Özkul A, Mitchell L, Nitsche A, Alten B, Linton YM. Co-circulation of West Nile virus and distinct insect-specific flaviviruses in Turkey. Parasit Vectors 2017 Mar 20;10(1):149.
    doi: 10.1186/s13071-017-2087-7pubmed: 28320443google scholar: lookup
  30. Khan SA, Chowdhury P, Choudhury P, Dutta P. Detection of West Nile virus in six mosquito species in synchrony with seroconversion among sentinel chickens in India. Parasit Vectors 2017 Jan 7;10(1):13.
    doi: 10.1186/s13071-016-1948-9pubmed: 28061903google scholar: lookup
  31. Khan J, Khan I, Amin I. A Comprehensive Entomological, Serological and Molecular Study of 2013 Dengue Outbreak of Swat, Khyber Pakhtunkhwa, Pakistan. PLoS One 2016;11(2):e0147416.
    doi: 10.1371/journal.pone.0147416pubmed: 26848847google scholar: lookup
  32. Conte A, Candeloro L, Ippoliti C, Monaco F, De Massis F, Bruno R, Di Sabatino D, Danzetta ML, Benjelloun A, Belkadi B, El Harrak M, Declich S, Rizzo C, Hammami S, Ben Hassine T, Calistri P, Savini G. Spatio-Temporal Identification of Areas Suitable for West Nile Disease in the Mediterranean Basin and Central Europe. PLoS One 2015;10(12):e0146024.
    doi: 10.1371/journal.pone.0146024pubmed: 26717483google scholar: lookup
  33. Ergunay K, Bakonyi T, Nowotny N, Ozkul A. Close relationship between West Nile virus from Turkey and lineage 1 strain from Central African Republic. Emerg Infect Dis 2015 Feb;21(2):352-5.
    doi: 10.3201/eid2102.141135pubmed: 25625703google scholar: lookup